##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936972_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 520696 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.13558967228479 33.0 31.0 34.0 31.0 34.0 2 32.23852881527801 33.0 31.0 34.0 30.0 34.0 3 32.32851798362192 34.0 31.0 34.0 30.0 34.0 4 36.03496858051531 37.0 35.0 37.0 35.0 37.0 5 35.88451802971407 37.0 35.0 37.0 35.0 37.0 6 36.00434034446203 37.0 35.0 37.0 35.0 37.0 7 35.96561525343002 37.0 35.0 37.0 35.0 37.0 8 35.93194493524052 37.0 35.0 37.0 35.0 37.0 9 37.59715457771905 39.0 37.0 39.0 35.0 39.0 10-11 37.52636855286002 39.0 37.0 39.0 35.0 39.0 12-13 37.40679110267796 39.0 37.0 39.0 34.0 39.0 14-15 38.46361792677493 40.0 38.0 41.0 34.0 41.0 16-17 38.490722993839015 40.0 38.0 41.0 34.0 41.0 18-19 38.71281611535329 40.0 38.0 41.0 34.5 41.0 20-21 38.754233756356875 40.0 38.0 41.0 35.0 41.0 22-23 38.660211524574805 40.0 38.0 41.0 34.0 41.0 24-25 38.542201207614426 40.0 38.0 41.0 34.0 41.0 26-27 38.28982073993271 40.0 38.0 41.0 34.0 41.0 28-29 38.096357567563416 40.0 37.0 41.0 33.5 41.0 30-31 37.93416600089112 40.0 37.0 41.0 33.5 41.0 32-33 37.651913016424174 40.0 36.0 41.0 33.0 41.0 34-35 37.449203565996285 40.0 35.0 41.0 33.0 41.0 36-37 37.22181080707361 40.0 35.0 41.0 33.0 41.0 38-39 36.973314563584125 39.0 35.0 41.0 32.5 41.0 40-41 36.49852793184507 39.0 35.0 41.0 30.5 41.0 42-43 36.204564659609446 39.0 35.0 41.0 30.0 41.0 44-45 35.78274655461152 39.0 35.0 40.5 29.5 41.0 46-47 35.081982577165945 39.0 35.0 40.0 25.5 41.0 48-49 34.441537864704166 38.0 34.5 40.0 22.5 41.0 50-51 34.05754125247745 38.0 34.0 40.0 19.5 41.0 52-53 33.724118295512156 38.0 34.0 40.0 16.5 41.0 54-55 33.36030524528708 38.0 33.0 40.0 12.5 41.0 56-57 33.12931345737244 38.0 33.0 40.0 9.0 41.0 58-59 32.811535905787636 37.0 33.0 40.0 7.5 41.0 60-61 32.40036604851967 36.5 32.0 39.5 6.0 41.0 62-63 32.00797106180958 36.0 31.5 39.0 2.0 40.0 64-65 31.66742494660992 35.5 31.0 39.0 2.0 40.0 66-67 31.354309424309 35.0 31.0 39.0 2.0 40.0 68-69 30.907496888779633 35.0 31.0 38.0 2.0 40.0 70-71 30.46483744833838 35.0 30.0 37.5 2.0 39.5 72-73 30.041440687080367 34.5 29.5 37.0 2.0 39.0 74-75 29.55011561448523 34.0 29.0 36.0 2.0 39.0 76-77 28.13829182478836 32.5 27.0 35.0 2.0 37.0 78-79 28.745309163120133 34.0 29.0 35.0 2.0 37.0 80-81 28.616404773610704 34.0 29.0 35.0 2.0 37.0 82-83 28.289610444482 34.0 28.5 35.0 2.0 36.5 84-85 27.86437671885323 34.0 27.0 35.0 2.0 36.0 86-87 27.462335988753516 33.0 26.5 35.0 2.0 36.0 88-89 27.064023921827708 33.0 26.0 35.0 2.0 35.0 90-91 26.59434583711033 33.0 25.0 35.0 2.0 35.0 92-93 26.113219613747752 32.5 24.5 34.5 2.0 35.0 94-95 25.601376811037532 32.0 23.0 34.0 2.0 35.0 96-97 24.864836680135816 32.0 18.5 34.0 2.0 35.0 98-99 24.05030190360594 31.5 2.0 34.0 2.0 35.0 100-101 21.213130118149554 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.07898625148448701 1101 2 0.1756404105956122 1101 3 0.17495114217327767 1101 4 0.13232914510212623 1101 5 -0.010367165131157208 1101 6 0.07799809893582221 1101 7 0.043923986180018915 1101 8 0.2268051779067406 1101 9 0.2663649690408434 1101 10-11 0.23373960606613053 1101 12-13 0.21299057871544846 1101 14-15 0.28447314847860383 1101 16-17 0.18767652747808938 1101 18-19 0.11219051682365944 1101 20-21 -0.025532944246421607 1101 22-23 0.05286059615207961 1101 24-25 0.06483117952059558 1101 26-27 0.13809849501506477 1101 28-29 0.23052839807137815 1101 30-31 0.13901901484529589 1101 32-33 0.2023999754391852 1101 34-35 -0.11539778388189603 1101 36-37 -0.1614268187142187 1101 38-39 0.05657543121054687 1101 40-41 0.12229381692672092 1101 42-43 0.05361745681973673 1101 44-45 -0.28044722800098043 1101 46-47 -0.6089946939163156 1101 48-49 -0.21148193161452156 1101 50-51 -0.20662027738837452 1101 52-53 -0.045084541403831224 1101 54-55 -0.21823858143295638 1101 56-57 -0.27447075019411926 1101 58-59 -0.2729231206063787 1101 60-61 0.04929964667942244 1101 62-63 -0.020931181951606703 1101 64-65 -0.11089674993850807 1101 66-67 0.10459871017327416 1101 68-69 -0.18967836033866448 1101 70-71 -0.1768299070053665 1101 72-73 -0.08003206153609455 1101 74-75 0.102883722113571 1101 76-77 0.03012630122627158 1101 78-79 0.022130607769611288 1101 80-81 0.10386707290853536 1101 82-83 0.4439418360363163 1101 84-85 0.20902955087327513 1101 86-87 -0.06792655335843278 1101 88-89 0.08431585660984098 1101 90-91 0.2958518677471318 1101 92-93 0.15976117399964096 1101 94-95 0.05344578920432497 1101 96-97 0.01791623551715915 1101 98-99 0.26453605685349046 1101 100-101 0.4507893701727994 1102 1 -0.10433125726735426 1102 2 -0.09761810025086959 1102 3 0.014074943551825925 1102 4 -0.023912021519841176 1102 5 -0.13255950989925935 1102 6 -0.11501166398537066 1102 7 -0.06620604114456086 1102 8 0.08110064579491905 1102 9 0.08380124188078497 1102 10-11 0.16467436771893773 1102 12-13 0.05806671790412565 1102 14-15 0.172052784778991 1102 16-17 0.32262336413216985 1102 18-19 0.13425674813314004 1102 20-21 0.014984628286931923 1102 22-23 -0.1488774502489818 1102 24-25 -0.11908790492468313 1102 26-27 -0.03790316475401312 1102 28-29 -0.09659559900046588 1102 30-31 0.007338263093124908 1102 32-33 -0.2077459972652207 1102 34-35 -0.011217376404964341 1102 36-37 -0.05034912847496287 1102 38-39 0.3645377928662583 1102 40-41 0.21495821741667953 1102 42-43 0.1396436869695492 1102 44-45 0.35595281258726175 1102 46-47 0.10713949370281739 1102 48-49 0.2725364490596789 1102 50-51 0.29560120393741585 1102 52-53 0.40281302402659236 1102 54-55 0.5312277919353576 1102 56-57 0.24756467754069433 1102 58-59 0.2741030660560426 1102 60-61 0.2653396115995861 1102 62-63 0.07176311612552766 1102 64-65 0.2591121649783972 1102 66-67 0.35088325547627264 1102 68-69 0.07647449062276834 1102 70-71 -0.016619609189159945 1102 72-73 0.1700096138884497 1102 74-75 0.10473012461844533 1102 76-77 0.2692653231946558 1102 78-79 -0.20468426637060588 1102 80-81 0.2177623842420715 1102 82-83 0.4241740949892865 1102 84-85 0.12888770947182238 1102 86-87 0.06768980805085079 1102 88-89 0.13771657125277414 1102 90-91 -0.036872038001178 1102 92-93 -0.07470114054471821 1102 94-95 -0.131752549577012 1102 96-97 0.07662241290226035 1102 98-99 0.10357016547051856 1102 100-101 0.1497251972668998 1103 1 0.12902779599982495 1103 2 0.24509029850380415 1103 3 0.24633716126808025 1103 4 0.07863873796587484 1103 5 0.0017748073710777135 1103 6 0.043719954486327595 1103 7 0.15376219772782918 1103 8 0.16646764831607186 1103 9 0.17829059651979406 1103 10-11 0.09455764108894016 1103 12-13 0.14033274875009738 1103 14-15 0.2523819700253185 1103 16-17 0.20371427811693366 1103 18-19 0.1747914386415843 1103 20-21 0.19529183196189592 1103 22-23 0.11357241831019138 1103 24-25 0.20951191383949208 1103 26-27 0.26470344586712 1103 28-29 0.21075947963972652 1103 30-31 0.12667127941331557 1103 32-33 0.3060104289798815 1103 34-35 0.18354831798838944 1103 36-37 0.015086420660573197 1103 38-39 -0.023444792345983956 1103 40-41 0.2704078527020428 1103 42-43 0.2505306209440903 1103 44-45 0.25100314589965933 1103 46-47 0.4704141197918048 1103 48-49 0.5402484366423295 1103 50-51 0.6977136778062558 1103 52-53 0.5081081154071185 1103 54-55 0.5108813803147285 1103 56-57 0.30646070919415536 1103 58-59 -0.00873919598222983 1103 60-61 0.05786722314484649 1103 62-63 0.19351298378749604 1103 64-65 0.4950731185299908 1103 66-67 0.5402870165679943 1103 68-69 0.44814360965522226 1103 70-71 0.6084319802130302 1103 72-73 0.5828459543605611 1103 74-75 0.3882318337977715 1103 76-77 0.7213937224876581 1103 78-79 0.7198864200824673 1103 80-81 0.5616954705444144 1103 82-83 0.8758617413461742 1103 84-85 0.6900895631014698 1103 86-87 0.5953316340465236 1103 88-89 0.9431134956271983 1103 90-91 0.6539773478606357 1103 92-93 0.5646653864567241 1103 94-95 0.6150904033106457 1103 96-97 0.8863337814120875 1103 98-99 0.8907863116075951 1103 100-101 1.1089583544485926 1104 1 0.012108490208206035 1104 2 0.10759275552046432 1104 3 0.17568203390789705 1104 4 -0.1231256890578365 1104 5 -0.061043882142953976 1104 6 -0.05510125563773727 1104 7 0.028864814306039932 1104 8 0.06352443141641828 1104 9 -0.13388348744658174 1104 10-11 -0.0646743822209217 1104 12-13 -0.006566496223129548 1104 14-15 0.002416657319365356 1104 16-17 0.02292699216644678 1104 18-19 0.1425423723079149 1104 20-21 0.07625949184141945 1104 22-23 -0.097749803526618 1104 24-25 -0.02160085455344074 1104 26-27 -0.11290677300970486 1104 28-29 -0.07522136141152913 1104 30-31 0.04582097821197806 1104 32-33 0.03478758624050471 1104 34-35 0.14605713434229273 1104 36-37 0.17548624953658987 1104 38-39 0.10365957015936544 1104 40-41 0.290020626876192 1104 42-43 0.4795346909199196 1104 44-45 0.4275860427512015 1104 46-47 -0.06022343267272845 1104 48-49 0.0011515203427734377 1104 50-51 -0.042839815782365065 1104 52-53 -0.015081785965342931 1104 54-55 0.22395675580469288 1104 56-57 0.14153274314145392 1104 58-59 0.09414331815283816 1104 60-61 0.15975536818398695 1104 62-63 0.33555600759453696 1104 64-65 0.23271577002674704 1104 66-67 0.14820352425122962 1104 68-69 0.14995862231608115 1104 70-71 0.38600693367424554 1104 72-73 0.43861618572481476 1104 74-75 0.2779638166391223 1104 76-77 0.5354597092833586 1104 78-79 0.6372367155025884 1104 80-81 0.2595633582038346 1104 82-83 0.39736208243790117 1104 84-85 0.3123608293033264 1104 86-87 0.1803863243467987 1104 88-89 0.43435928979279126 1104 90-91 0.41503392283722107 1104 92-93 0.5441868752353827 1104 94-95 0.45082438645262 1104 96-97 0.6407130522851681 1104 98-99 0.3735264922127719 1104 100-101 1.1094757732514218 1105 1 -0.019568018978148416 1105 2 0.036066344759312585 1105 3 -0.01882666031782776 1105 4 0.0015462269526622663 1105 5 -0.029695456946100762 1105 6 -0.028485441989445803 1105 7 -0.02845145865983767 1105 8 0.04908053377862842 1105 9 0.1295433498237628 1105 10-11 0.013813560692469196 1105 12-13 0.15679062319944137 1105 14-15 0.13702541716188676 1105 16-17 -0.13251920205927803 1105 18-19 -0.010137663781321749 1105 20-21 0.10963998724823654 1105 22-23 0.004503723376281243 1105 24-25 0.09943137969852955 1105 26-27 -0.18557532615930938 1105 28-29 -0.19484693555850896 1105 30-31 0.11502824527234878 1105 32-33 -0.08010947740778818 1105 34-35 -0.2292545454477306 1105 36-37 -0.02325677867863618 1105 38-39 0.07799725060556284 1105 40-41 -0.0789795371658002 1105 42-43 0.11089336874144351 1105 44-45 0.19278453356485414 1105 46-47 0.2961988114217604 1105 48-49 0.5964640203427791 1105 50-51 0.7145707813304867 1105 52-53 0.412819706816812 1105 54-55 0.8649631534424884 1105 56-57 0.6955025594144217 1105 58-59 0.8484125110662291 1105 60-61 0.6604484456118058 1105 62-63 0.7988475102192147 1105 64-65 0.6107669511291114 1105 66-67 0.5822955518102866 1105 68-69 0.4574635435759262 1105 70-71 0.6310159559839548 1105 72-73 0.6408615105279658 1105 74-75 0.371970132255921 1105 76-77 0.35997783592377885 1105 78-79 0.574591128232246 1105 80-81 0.1828511698836195 1105 82-83 0.0899104748263504 1105 84-85 -0.15993452830821653 1105 86-87 0.09127051069850722 1105 88-89 0.17751258703689032 1105 90-91 0.3860292476403693 1105 92-93 0.10832934570782626 1105 94-95 0.27214574472033704 1105 96-97 0.18389710740327914 1105 98-99 0.2372896155723545 1105 100-101 0.6750720659023379 1106 1 -0.10592554585986846 1106 2 0.1586637909958668 1106 3 0.02050465688647307 1106 4 0.11982579684513439 1106 5 0.013920134451744559 1106 6 -0.03967495811847499 1106 7 -0.024822426401776454 1106 8 0.05879155528400304 1106 9 0.1589452315441946 1106 10-11 0.06941671929460824 1106 12-13 0.11819855330696782 1106 14-15 0.3344884547962934 1106 16-17 0.17097542159663703 1106 18-19 0.05003538729394563 1106 20-21 -0.17791881651520214 1106 22-23 -0.002309112645228595 1106 24-25 0.035041258730785785 1106 26-27 0.24627111201273522 1106 28-29 0.09818901874256625 1106 30-31 -0.16446604236205786 1106 32-33 0.08925376183952238 1106 34-35 0.07390405670280842 1106 36-37 0.209496917557928 1106 38-39 0.017807398455019552 1106 40-41 0.3348813499309742 1106 42-43 0.18806910798875265 1106 44-45 0.29342297442507004 1106 46-47 0.4360173598088579 1106 48-49 0.05222678915997392 1106 50-51 0.28206070068532796 1106 52-53 0.008997462193157446 1106 54-55 0.631377803980115 1106 56-57 0.270636968016575 1106 58-59 0.2760838820339657 1106 60-61 0.31352304238599515 1106 62-63 0.11482533279985674 1106 64-65 0.0840196393011503 1106 66-67 0.42058520234791885 1106 68-69 0.3960724145436707 1106 70-71 0.25576393985492274 1106 72-73 0.5905338895602235 1106 74-75 0.05109479623441615 1106 76-77 0.31679067194528443 1106 78-79 0.2411821900602007 1106 80-81 0.005994651066416168 1106 82-83 0.1155993189123734 1106 84-85 0.04780236685307315 1106 86-87 0.36872346768775444 1106 88-89 0.8187340252089292 1106 90-91 0.6615752825866075 1106 92-93 0.21571341828847324 1106 94-95 0.6503883522472229 1106 96-97 0.8139710321344644 1106 98-99 0.6072492929917068 1106 100-101 1.0138657486980343 1107 1 0.30858309522798066 1107 2 0.30077299517714096 1107 3 0.37197678675737933 1107 4 0.28566878962675446 1107 5 0.2601896405469333 1107 6 0.2161611457542847 1107 7 0.2484635831228914 1107 8 0.12306207973962557 1107 9 0.19793645567334295 1107 10-11 0.20169874523285358 1107 12-13 0.33564246860333924 1107 14-15 0.4498866636799832 1107 16-17 0.3974947255173191 1107 18-19 0.3481514204805194 1107 20-21 0.11516531802818264 1107 22-23 0.1024472296436798 1107 24-25 0.3173217870801466 1107 26-27 0.34415352133372323 1107 28-29 0.20653058881475062 1107 30-31 -0.025268150967207248 1107 32-33 -0.37435782350806335 1107 34-35 -0.054097860210966076 1107 36-37 0.30007408831301063 1107 38-39 0.217675610633421 1107 40-41 0.10679257286205512 1107 42-43 -0.19281267234491395 1107 44-45 -0.2954379594713572 1107 46-47 -0.15126811058381406 1107 48-49 -0.07475377213633294 1107 50-51 -0.47385627298705657 1107 52-53 -0.44499061699934117 1107 54-55 -0.5103719788694931 1107 56-57 -0.5745183319504861 1107 58-59 -0.7516005460368405 1107 60-61 -0.8159225502099225 1107 62-63 -1.2186098365774463 1107 64-65 -0.9392678678123936 1107 66-67 -0.8080726584961191 1107 68-69 -0.9012498965354965 1107 70-71 -1.1611019061330374 1107 72-73 -0.9202217937895867 1107 74-75 -0.8965165494989513 1107 76-77 -1.1158208235469722 1107 78-79 -0.3853910270602334 1107 80-81 -0.823570748640055 1107 82-83 -0.9103898385441234 1107 84-85 -0.7530512588096094 1107 86-87 -0.7294554642318545 1107 88-89 -0.46054233984333237 1107 90-91 -0.5217984856465385 1107 92-93 -0.42357133326153473 1107 94-95 -0.40486069678383885 1107 96-97 -0.506518978947696 1107 98-99 -0.48494227857806393 1107 100-101 0.3871847051781039 1108 1 -0.007691498296878763 1108 2 0.07678638733838739 1108 3 0.08100226303983504 1108 4 0.14996471843684844 1108 5 -0.1355500798171505 1108 6 0.03542118818086948 1108 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12 2125.0 13 2924.0 14 3634.0 15 5369.0 16 7705.0 17 11137.0 18 9819.0 19 5037.0 20 3819.0 21 3700.0 22 3695.0 23 3972.0 24 4523.0 25 5377.0 26 6159.0 27 7277.0 28 8972.0 29 10688.0 30 13176.0 31 16355.0 32 20542.0 33 26698.0 34 36819.0 35 52324.0 36 75402.0 37 103587.0 38 63644.0 39 3255.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.95630325708622 19.66641566843646 15.537430751381132 18.83985032309619 2 15.725516030707357 22.25457450874568 40.38877802468804 21.631131435858926 3 19.39476696396334 28.75577116606362 31.18542247624315 20.664039393729883 4 12.382080907093583 18.0418132653218 37.876995406148694 31.699110421435922 5 12.2057504575668 40.00564636472059 32.4592991209724 15.329304056740204 6 28.10987601210687 38.2557192680566 17.593182970485657 16.04122174935087 7 25.95867430520479 36.86836581248451 20.245326900880173 16.927632981430516 8 27.130225697912024 33.005438874122326 19.423425568853993 20.44090985911165 9 28.488462535649468 16.609915593581658 20.630119359701936 34.271502511066934 10-11 24.476469955597892 28.121879177101416 25.959388971684056 21.442261895616635 12-13 27.709706012563107 26.395871326597543 27.093170767778446 18.801251893060904 14-15 24.152709610322766 25.9273307988004 28.721590936371953 21.19836865450488 16-17 20.125274632415074 29.651946625286158 28.8327546207384 21.390024121560373 18-19 19.897021952453795 26.546327157915357 34.45414624918498 19.102504640445865 20-21 21.84777358570286 24.968239350940046 34.39301390554676 18.790973157810335 22-23 20.980166587604455 25.67482960012445 34.17187292941825 19.173130882852846 24-25 20.643357234287933 25.598220055868122 34.80532101123412 18.953101698609824 26-27 20.69178760290343 25.51755395918336 34.92047640218976 18.87018203572344 28-29 19.88546066839577 25.67081081132987 35.058753261269324 19.384975259005042 30-31 19.930727334183477 26.16392290319111 34.83337686481172 19.071972897813698 32-33 19.808141042260825 26.221396402884604 34.41506064010601 19.555401914748558 34-35 20.28131577734417 25.762537065773504 34.46915282621721 19.486994330665112 36-37 20.71343999339336 25.755898200171508 34.2772118070593 19.253449999375828 38-39 20.926869041525293 25.70478906027165 34.28883410530804 19.079507792895015 40-41 20.318767182613186 26.00570201087588 34.2383763192742 19.437154487236732 42-43 20.150337240923687 26.720581682978167 33.53511453900164 19.593966537096502 44-45 20.43839778448467 27.10366887110698 32.72590391815499 19.73202942625336 46-47 20.82382279820509 27.161896275071086 31.64504330274278 20.36923762398105 48-49 21.17686824641273 27.465380438279187 30.932089868262736 20.425661447045346 50-51 21.55771424620597 27.94611339655762 30.334404996485404 20.16176736075101 52-53 21.105718724853226 28.29287123122454 30.522784058936843 20.078625984985393 54-55 20.57428902853104 28.139835911933258 30.419669058337302 20.866206001198396 56-57 21.122962815012272 28.909397842110724 29.543935593650012 20.42370374922699 58-59 21.122305529522023 28.067624871326068 29.948568838631374 20.861500760520535 60-61 21.212386015318312 27.663995820881766 30.61108080908958 20.512537354710343 62-63 21.76769170494876 27.695814832455024 30.38980518383087 20.146688278765346 64-65 21.46196769513084 27.934944703766405 30.484515422161323 20.118572178941434 66-67 21.035594665601426 28.114293176824866 30.2522009003334 20.59791125724031 68-69 21.204852365446534 28.565694471518327 29.951055753421635 20.278397409613508 70-71 21.21378857366735 28.53222250389866 29.592446206202517 20.661542716231477 72-73 21.151502755459788 28.37542623443542 29.1841553620316 21.288915648073196 74-75 21.325401283916474 28.684057636998304 29.380272628187587 20.610268450897635 76-77 21.342031955336658 28.34468513747478 29.529638723591813 20.783644183596746 78-79 21.09488805773081 28.227696769206396 29.5524709881552 21.124944184907598 80-81 20.89737273983508 29.224440174350434 29.27514353949821 20.603043546316275 82-83 21.026780527198717 28.945900243040317 28.867351455889285 21.159967773871678 84-85 20.96514451816236 28.56935709847824 28.682958870012875 21.782539513346528 86-87 21.11793428943186 28.291938140073075 28.872030997181636 21.718096573313424 88-89 21.435423790825574 27.799560378777183 29.13887824566357 21.62613758473368 90-91 20.805825419856784 28.072811986552292 29.51149786770658 21.609864725884343 92-93 20.51360102631862 28.454414860110315 29.13955551799899 21.89242859557208 94-95 20.328752285402615 28.73778557930155 28.77763608708344 22.155826048212393 96-97 20.447518120047476 28.488421222445425 28.585023065370446 22.47903759213665 98-99 20.421401155962794 28.67456065635794 28.396757004867567 22.507281182811703 100-101 20.64110763486768 29.22675670329206 28.25360060075632 21.878535061083934 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1231.0 1 843.0 2 548.0 3 631.0 4 533.5 5 496.0 6 859.0 7 1891.5 8 3044.0 9 3382.0 10 2702.5 11 1961.5 12 1867.0 13 1953.5 14 2021.5 15 2057.0 16 2097.0 17 2126.0 18 2124.0 19 2181.0 20 2342.0 21 2568.0 22 2848.5 23 3264.5 24 3557.5 25 3935.0 26 4618.0 27 5314.5 28 5974.0 29 6638.0 30 7368.5 31 8164.0 32 9095.5 33 10113.0 34 11129.5 35 11860.5 36 12800.0 37 13868.5 38 14230.0 39 14044.5 40 13964.5 41 14415.0 42 14934.5 43 15387.5 44 16069.5 45 16786.0 46 17002.5 47 17569.5 48 24201.0 49 24148.5 50 16982.5 51 15017.5 52 13884.5 53 13503.0 54 13017.5 55 12190.5 56 11163.0 57 10008.0 58 9319.0 59 8867.5 60 7935.0 61 6766.5 62 5631.0 63 4151.5 64 2771.0 65 1986.0 66 1415.0 67 1089.5 68 814.0 69 506.5 70 356.5 71 274.0 72 232.0 73 203.0 74 146.0 75 116.0 76 92.0 77 36.5 78 19.5 79 17.0 80 12.0 81 8.0 82 6.5 83 5.0 84 1.0 85 0.0 86 0.0 87 0.5 88 2.0 89 1.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020741469110575075 2 0.007489974956596556 3 7.682025596509287E-4 4 0.0 5 0.0013443544793891253 6 0.0 7 5.761519197381966E-4 8 0.0 9 1.9205063991273218E-4 10-11 0.0 12-13 0.008546253476116582 14-15 0.004129088758123742 16-17 0.0 18-19 4.801265997818304E-4 20-21 8.642278796072948E-4 22-23 0.0017284557592145897 24-25 0.001056278519520027 26-27 2.880759598690983E-4 28-29 2.880759598690983E-4 30-31 0.0 32-33 1.9205063991273218E-4 34-35 0.0 36-37 0.0014403797993454914 38-39 0.011811114354633029 40-41 4.801265997818304E-4 42-43 0.0 44-45 8.642278796072948E-4 46-47 2.880759598690983E-4 48-49 9.602531995636609E-5 50-51 0.0019205063991273217 52-53 5.761519197381966E-4 54-55 0.0 56-57 3.8410127982546437E-4 58-59 0.0 60-61 0.0023046076789527865 62-63 0.0 64-65 9.602531995636609E-5 66-67 0.0 68-69 2.880759598690983E-4 70-71 7.682025596509287E-4 72-73 4.801265997818304E-4 74-75 0.0045131900379492065 76-77 9.602531995636609E-5 78-79 6.721772396945626E-4 80-81 0.00393703811821101 82-83 9.602531995636609E-5 84-85 0.003360886198472813 86-87 6.721772396945626E-4 88-89 0.00393703811821101 90-91 0.002400632998909152 92-93 0.0 94-95 0.0 96-97 3.8410127982546437E-4 98-99 8.642278796072948E-4 100-101 0.0036489621583419116 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 520696.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.63755796095078 #Duplication Level Percentage of deduplicated Percentage of total 1 81.34407316822465 48.51161878349819 2 11.243450413375223 13.410638514174819 3 3.2275194945433046 5.774441427777747 4 1.3865169826912107 3.307539476763587 5 0.7323008226108987 2.1836316376654707 6 0.43107927449769723 1.542510913117262 7 0.2996566994091146 1.2509555645578796 8 0.208976943222529 0.9970299651148715 9 0.15789606419796018 0.8474882112370672 >10 0.870729486868159 9.499134078170297 >50 0.0635865618752352 2.648150748734697 >100 0.027113051251484895 2.930348060421227 >500 0.003873293035926406 1.4554530437393989 >1k 0.0025821953572842705 2.9390103255935913 >5k 6.455488393210676E-4 2.70204924943393 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7963 1.5292992456250865 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6074 1.1665155868299353 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4398 0.8446387143361961 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2744 0.5269869559205371 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1870 0.3591346966368092 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1432 0.2750165163550325 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1312 0.25197043956550463 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1197 0.22988461597554044 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1175 0.2256595018974603 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1140 0.21893772950051468 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 847 0.16266689200608417 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 773 0.148455144652542 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 742 0.14250157481524728 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 700 0.13443544793891252 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 681 0.13078648578057062 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 658 0.12636932106257778 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 608 0.11676678906694117 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 521 0.10005838339453346 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 9.602531995636609E-5 0.0 0.0 0.0 0.0 20-21 1.9205063991273218E-4 0.0 0.0 0.0 0.0 22-23 1.9205063991273218E-4 0.0 0.0 2.8807595986909826E-4 0.0 24-25 1.9205063991273218E-4 0.0 0.0 9.60253199563661E-4 0.0 26-27 1.9205063991273218E-4 0.0 0.0 0.0014403797993454914 0.0 28-29 2.8807595986909826E-4 0.0 0.0 0.0027847342787346166 0.0 30-31 3.8410127982546437E-4 0.0 0.0 0.006337671117120162 0.0 32-33 3.8410127982546437E-4 0.0 0.0 0.009410481355723877 0.0 34-35 5.761519197381965E-4 0.0 0.0 0.014403797993454915 0.0 36-37 5.761519197381965E-4 0.0 0.0 0.019589165271098684 0.0 38-39 5.761519197381965E-4 0.0 0.0 0.024486456588873357 0.0 40-41 5.761519197381965E-4 0.0 0.0 0.028999646626822563 0.0 42-43 6.721772396945626E-4 0.0 0.0 0.045419976339361165 0.0 44-45 7.682025596509287E-4 0.0 0.0 0.06462504033063438 0.0 46-47 9.602531995636608E-4 0.0 0.0 0.07941293960391477 0.0 48-49 0.001152303839476393 0.0 0.0 0.10293914299322446 0.0 50-51 0.001152303839476393 0.0 0.0 0.13328314409943615 0.0 52-53 0.001152303839476393 0.0 0.0 0.1951234501513359 0.0 54-55 0.001152303839476393 0.0 0.0 0.23747061625209337 0.0 56-57 0.0013443544793891253 0.0 0.0 0.3118902392182771 0.0 58-59 0.0013443544793891253 0.0 0.0 0.4021540399772612 0.0 60-61 0.0013443544793891253 0.0 0.0 0.49597077757463087 0.0 62-63 0.0013443544793891253 0.0 0.0 0.6403928587890055 0.0 64-65 0.0013443544793891253 0.0 0.0 0.701657012921167 0.0 66-67 0.0013443544793891253 9.602531995636609E-5 0.0 0.8549134235715273 0.0 68-69 0.0013443544793891253 1.9205063991273218E-4 0.0 1.052053405441947 0.0 70-71 0.0013443544793891253 1.9205063991273218E-4 0.0 1.179286954384132 0.0 72-73 0.0013443544793891253 1.9205063991273218E-4 0.0 1.4370189131470186 0.0 74-75 0.0013443544793891253 1.9205063991273218E-4 0.0 1.6782345168774104 0.0 76-77 0.0013443544793891253 1.9205063991273218E-4 0.0 1.7990343693825188 0.0 78-79 0.0013443544793891253 1.9205063991273218E-4 0.0 1.9279963740839183 0.0 80-81 0.0013443544793891253 1.9205063991273218E-4 0.0 2.210406840075591 0.0 82-83 0.0013443544793891253 1.9205063991273218E-4 0.0 2.4231989490988983 0.0 84-85 0.0014403797993454914 1.9205063991273218E-4 0.0 2.8059558744449733 0.0 86-87 0.0015364051193018575 1.9205063991273218E-4 0.0 3.3557008311951693 0.0 88-89 0.0015364051193018575 1.9205063991273218E-4 0.0 4.113244580330942 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCGTAA 30 0.003952113 31.66449 60-61 CTTAACG 65 0.005837654 29.234373 9 GTATCAA 8805 0.0 26.927856 1 GGTATCA 6265 0.0 24.345268 1 AGTACTT 5600 0.0 23.794205 12-13 GAGTACT 5560 0.0 23.153723 12-13 GTACATG 4640 0.0 22.93827 1 CGGGTCG 125 2.2531857E-4 22.800621 6 GCACTTA 125 2.2531857E-4 22.800621 6 CGGTTAC 85 3.1503932E-6 22.351404 28-29 ATCAACG 10650 0.0 22.209742 3 TACCTGG 365 0.0 22.123892 2 TACATGG 4820 0.0 22.075293 2 TATCAAC 10785 0.0 21.977882 2 TCAACGC 10770 0.0 21.918177 4 CAACGCA 10915 0.0 21.627007 5 GTGTAGC 200 5.3581425E-7 21.381744 1 GTACTTT 6040 0.0 21.31267 14-15 AACGCAG 11185 0.0 21.10697 6 ACATGGG 4875 0.0 20.947277 3 >>END_MODULE