FastQCFastQC Report
Thu 26 May 2016
SRR936971_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936971_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1258633
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79680.633067780679515No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55640.44206690909899865No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39740.3157393775628003No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13210.10495513783604911No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA86050.033.8645741
GGTATCA55350.032.206961
ATCAACG105500.027.4817453
TCAACGC105900.027.3779434
TATCAAC105800.027.364342
CAACGCA106700.027.084665
AACGCAG109300.026.6143266
GTACATG71400.026.0990031
TACATGG72650.024.80032
ACGCAGA119550.024.2131847
CGCAGAG120600.023.9235488
ACATGGG72450.023.7485493
GAGTACT58800.023.71934312-13
GCAGAGT124850.022.5381039
GTACTTT61800.022.10656514-15
AGTACTT61000.021.81223112-13
GTGTAGC5900.020.9487151
ATACCGT1150.003709062520.6659986
CATGGGA45150.020.4225944
CATGGGG28050.019.9947554
Produced by FastQC (version 0.11.5)