FastQCFastQC Report
Thu 26 May 2016
SRR936971_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936971_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1258633
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84080.6680263428656328No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65460.5200880637961979No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46420.3688128310635428No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16460.13077680308715883No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA13100.10408117378139616No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA92850.031.9824281
GGTATCA60850.028.890461
ATCAACG114500.025.7619443
TCAACGC115250.025.635514
TATCAAC115500.025.5409262
CAACGCA116750.025.2654615
AACGCAG118950.024.7572566
GTACATG64700.023.1323411
GAGTACT67800.022.41793312-13
TACATGG67250.022.3920572
AGTACTT70550.022.3856612-13
ACGCAGA131700.022.3244197
CGCAGAG131100.022.245448
ACATGGG65700.022.0509343
GCAGAGT135750.021.2385149
GTACTTT71550.021.20979714-15
GACAACG1150.003724383720.65137
AGAGTAC119800.019.58598510-11
TACTTTT79750.019.56500414-15
CATTCCG1707.957201E-519.5579999
Produced by FastQC (version 0.11.5)