##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936971_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1258633 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.10923041108886 31.0 31.0 34.0 31.0 34.0 2 32.10404621521921 33.0 31.0 34.0 30.0 34.0 3 32.27668510201147 33.0 31.0 34.0 30.0 34.0 4 36.02222649493538 37.0 35.0 37.0 35.0 37.0 5 35.95651313766602 37.0 35.0 37.0 35.0 37.0 6 36.00338065186595 37.0 35.0 37.0 35.0 37.0 7 35.94459782955 37.0 35.0 37.0 35.0 37.0 8 35.919499965438696 37.0 35.0 37.0 35.0 37.0 9 37.600276649348935 39.0 37.0 39.0 35.0 39.0 10-11 37.52273975018929 39.0 37.0 39.0 35.0 39.0 12-13 37.42335374966332 39.0 37.0 39.0 34.0 39.0 14-15 38.59510516568372 40.0 38.0 41.0 34.0 41.0 16-17 38.51134246440384 40.0 38.0 41.0 34.0 41.0 18-19 38.588931006893986 40.0 38.0 41.0 34.0 41.0 20-21 38.68163475770936 40.0 38.0 41.0 34.0 41.0 22-23 38.614536961926156 40.0 38.0 41.0 34.0 41.0 24-25 38.510151887007574 40.0 38.0 41.0 34.0 41.0 26-27 38.343206876031374 40.0 38.0 41.0 34.0 41.0 28-29 38.157685361817144 40.0 38.0 41.0 33.5 41.0 30-31 38.004206547897596 40.0 37.0 41.0 33.0 41.0 32-33 37.96479752239136 40.0 37.0 41.0 33.0 41.0 34-35 37.87379482343145 40.0 37.0 41.0 33.0 41.0 36-37 37.69491384700703 40.0 37.0 41.0 33.0 41.0 38-39 37.529648038784934 40.0 37.0 41.0 33.0 41.0 40-41 37.33660010503459 40.0 36.0 41.0 32.5 41.0 42-43 37.08953006952781 40.0 36.0 41.0 31.5 41.0 44-45 36.71733261403443 39.0 36.0 41.0 31.0 41.0 46-47 36.39109732543164 39.0 35.5 41.0 30.0 41.0 48-49 36.0161468831661 39.0 35.0 41.0 29.5 41.0 50-51 35.736644041591155 39.0 35.0 40.0 28.5 41.0 52-53 35.47280104685004 39.0 35.0 40.0 28.0 41.0 54-55 35.22444390064459 38.0 35.0 40.0 27.0 41.0 56-57 34.96226262937647 38.0 34.0 40.0 26.5 41.0 58-59 34.63731564324152 38.0 34.0 40.0 26.0 41.0 60-61 34.31564602231151 37.5 34.0 40.0 26.0 41.0 62-63 33.94244549443722 37.0 33.0 40.0 25.0 41.0 64-65 33.537566153120096 36.5 33.0 39.0 23.0 41.0 66-67 33.16153000914484 36.0 32.5 39.0 22.5 40.0 68-69 32.73171289804097 35.5 32.0 39.0 22.0 40.0 70-71 32.2614713741019 35.0 31.5 38.0 21.0 40.0 72-73 31.923330311536407 35.0 31.0 37.5 20.0 39.5 74-75 31.479989003943167 35.0 31.0 37.0 18.5 39.0 76-77 30.075152963572386 33.5 29.5 35.5 18.0 38.0 78-79 30.790608541171256 34.0 31.0 36.0 18.0 38.0 80-81 30.624181155269248 34.0 31.0 36.0 16.0 37.0 82-83 30.29776193695859 34.0 30.5 35.0 11.5 37.0 84-85 29.91159217976964 34.0 30.0 35.0 7.0 36.5 86-87 29.554691478771016 34.0 30.0 35.0 4.5 36.0 88-89 29.185799990942556 34.0 29.0 35.0 2.0 36.0 90-91 28.752796883603082 34.0 29.0 35.0 2.0 35.0 92-93 28.303722371811322 34.0 29.0 35.0 2.0 35.0 94-95 27.832392365367824 33.0 28.0 35.0 2.0 35.0 96-97 27.195646387787384 33.0 26.5 35.0 2.0 35.0 98-99 26.467044007268203 33.0 25.0 34.5 2.0 35.0 100-101 23.46603974311813 29.5 12.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2305331740176726 1101 2 0.21912206159521475 1101 3 0.1539485992226588 1101 4 0.17402935718895662 1101 5 0.2624148277043403 1101 6 0.18842706449760271 1101 7 0.2843257576758944 1101 8 0.2273706266412603 1101 9 0.3184392736141888 1101 10-11 0.3379480109842561 1101 12-13 0.33718726751269656 1101 14-15 0.646275371168862 1101 16-17 0.5398229994714967 1101 18-19 0.5674454755740399 1101 20-21 0.5845822311651574 1101 22-23 0.4016511077778162 1101 24-25 0.4485876803692008 1101 26-27 0.44050018002886304 1101 28-29 0.4619981925221879 1101 30-31 0.459960876958057 1101 32-33 0.5915674144402843 1101 34-35 0.5686579784953167 1101 36-37 0.5264863929443848 1101 38-39 0.47726150160182357 1101 40-41 0.3616765890361293 1101 42-43 0.4006471008678929 1101 44-45 0.40674906661345034 1101 46-47 0.4141374510030147 1101 48-49 0.2357581500393522 1101 50-51 0.3025870012608962 1101 52-53 0.27170168394356153 1101 54-55 0.3822384668525274 1101 56-57 0.5020946136256796 1101 58-59 0.44462623660771783 1101 60-61 0.23074302734298868 1101 62-63 0.3391893336559306 1101 64-65 0.3218108853057089 1101 66-67 0.40636221697161545 1101 68-69 0.31878775292443606 1101 70-71 0.2278039024298124 1101 72-73 0.32581778036335507 1101 74-75 0.35197152162276524 1101 76-77 0.3422188135609936 1101 78-79 0.38522090877366466 1101 80-81 0.5000509012320045 1101 82-83 0.6150073954427455 1101 84-85 0.7080371620628831 1101 86-87 0.451635898484092 1101 88-89 0.5574493540480923 1101 90-91 0.7436585238294633 1101 92-93 0.8311974062054652 1101 94-95 0.8364288206099495 1101 96-97 0.8682638652768233 1101 98-99 0.8062728563126171 1101 100-101 1.1241760726639747 1102 1 0.21342530096305268 1102 2 0.06089171880200439 1102 3 0.2063720556360451 1102 4 0.17498933074200806 1102 5 0.09384728625382621 1102 6 0.1633914628294093 1102 7 0.19954292785187278 1102 8 0.2528067470766189 1102 9 0.3115795950463962 1102 10-11 0.24124497975650172 1102 12-13 0.28858145426887205 1102 14-15 0.3770282193023107 1102 16-17 0.46761836616867924 1102 18-19 0.40011445634913656 1102 20-21 0.263528294637851 1102 22-23 0.3336392066487406 1102 24-25 0.42828519333837534 1102 26-27 0.41417719660906727 1102 28-29 0.39510520096486346 1102 30-31 0.32500830346604204 1102 32-33 0.49488663425514545 1102 34-35 0.48649123553936846 1102 36-37 0.5356061416983167 1102 38-39 0.5580677522375694 1102 40-41 0.4175394208945349 1102 42-43 0.5483717728239483 1102 44-45 0.7557820998755815 1102 46-47 0.6909690296827051 1102 48-49 0.540451212800015 1102 50-51 0.6864174864607762 1102 52-53 0.763224036705239 1102 54-55 0.6674046503536957 1102 56-57 0.7775084830184227 1102 58-59 0.7549233833883093 1102 60-61 0.6369993847837421 1102 62-63 0.8079329762151772 1102 64-65 0.7547716726111702 1102 66-67 0.6216791989673425 1102 68-69 0.6648264315939514 1102 70-71 0.6002768197322226 1102 72-73 0.7593698096584482 1102 74-75 0.6770370032624768 1102 76-77 0.7112714277406305 1102 78-79 0.6831560120185038 1102 80-81 0.8173793774806022 1102 82-83 0.7247749674089725 1102 84-85 0.6097002685627295 1102 86-87 0.47146475617914163 1102 88-89 0.663033730001704 1102 90-91 0.8722472991320807 1102 92-93 0.6274414302111637 1102 94-95 0.8134689233461962 1102 96-97 0.8622242965656035 1102 98-99 0.7934212898392161 1102 100-101 0.8471569494822013 1103 1 -0.05035998046500367 1103 2 -0.28410671679394994 1103 3 -0.10729507211812717 1103 4 -0.03542918850839527 1103 5 0.09945238127078682 1103 6 0.09558278121033226 1103 7 0.21490702230894243 1103 8 0.2344599928033233 1103 9 0.10843555460243692 1103 10-11 0.18679342687964606 1103 12-13 0.1543957265739877 1103 14-15 0.1278958460190438 1103 16-17 0.3133753318835346 1103 18-19 0.35507299964196903 1103 20-21 0.22183383921512956 1103 22-23 0.3399091733845694 1103 24-25 0.2655161570112696 1103 26-27 0.25513597044564307 1103 28-29 0.13888051960927328 1103 30-31 0.1593368859513049 1103 32-33 0.2246224558142842 1103 34-35 0.08682408308407474 1103 36-37 0.0958470187526288 1103 38-39 0.1215485924048707 1103 40-41 0.01704992710275377 1103 42-43 -0.056267302695601984 1103 44-45 0.09180476726681519 1103 46-47 0.11084992913269076 1103 48-49 0.0019812822924833995 1103 50-51 -0.16757215553770521 1103 52-53 -0.032581096186774516 1103 54-55 0.038041815653564015 1103 56-57 -0.011892885423428368 1103 58-59 0.09942059216214716 1103 60-61 0.06798343609619195 1103 62-63 0.03096038622077657 1103 64-65 -0.04198136562615673 1103 66-67 -0.0335683473884103 1103 68-69 0.060459068355797285 1103 70-71 -0.011863161494929386 1103 72-73 -0.044960296062338756 1103 74-75 -0.08245131310942355 1103 76-77 -0.006379372575540998 1103 78-79 0.05529068250561053 1103 80-81 0.1402301745032979 1103 82-83 0.21425640863678197 1103 84-85 0.12678520746863597 1103 86-87 0.020086845564247113 1103 88-89 0.3611531516662332 1103 90-91 0.45958941782917506 1103 92-93 0.5251417562658993 1103 94-95 0.439134824278252 1103 96-97 0.5701047760966098 1103 98-99 0.41408933153470073 1103 100-101 0.5157626537933702 1104 1 0.30510973260840046 1104 2 0.11178838272601155 1104 3 0.17038394882177244 1104 4 0.14944671972646972 1104 5 0.17366969880026772 1104 6 0.18398948811177718 1104 7 0.20477892156867483 1104 8 0.3000787320169067 1104 9 0.19883587200553166 1104 10-11 0.1879422520531122 1104 12-13 0.18941469410231804 1104 14-15 0.33402372507437406 1104 16-17 0.42069310997899123 1104 18-19 0.3037480178222012 1104 20-21 0.291971739672384 1104 22-23 0.28261320121328026 1104 24-25 0.31250973643559377 1104 26-27 0.22278058878499962 1104 28-29 0.20958584025476767 1104 30-31 0.16439653370016316 1104 32-33 0.2656926967878732 1104 34-35 0.21550752321982003 1104 36-37 0.07431431608184624 1104 38-39 0.15485494025960378 1104 40-41 0.3148188858258507 1104 42-43 0.28683622416660626 1104 44-45 0.529319917463539 1104 46-47 0.40266757981110857 1104 48-49 0.4178619515737907 1104 50-51 0.5004134846655788 1104 52-53 0.47408732116929286 1104 54-55 0.22856058946657498 1104 56-57 0.16562106311666014 1104 58-59 0.07347672195763977 1104 60-61 -0.054501631248378146 1104 62-63 0.20532697850904214 1104 64-65 0.07172850059630775 1104 66-67 0.1739261571043329 1104 68-69 0.2026557070321502 1104 70-71 0.14943204824594147 1104 72-73 0.2878592254711485 1104 74-75 0.19415761188441394 1104 76-77 0.11041543626782158 1104 78-79 0.026612266537664908 1104 80-81 0.20895612842183553 1104 82-83 0.228689496044872 1104 84-85 0.22780309350600447 1104 86-87 0.3765826283710254 1104 88-89 0.30562729222276275 1104 90-91 0.20687015175132117 1104 92-93 0.15930930863637727 1104 94-95 0.32162756772248713 1104 96-97 0.7090590136972743 1104 98-99 0.641835375908979 1104 100-101 0.9162346665889451 1105 1 0.17779812908387527 1105 2 -0.021614629767896076 1105 3 0.09918981826636042 1105 4 0.11755983328143316 1105 5 0.014414982057090242 1105 6 0.09627204288821645 1105 7 0.18661189336911121 1105 8 0.12595229639498484 1105 9 0.26096645684805253 1105 10-11 0.27144663038468053 1105 12-13 0.29606190735628246 1105 14-15 0.33971366437048545 1105 16-17 0.3831656797212091 1105 18-19 0.2907478097305898 1105 20-21 0.3168145148998107 1105 22-23 0.4906075974533337 1105 24-25 0.34354849192164494 1105 26-27 0.26743752138853694 1105 28-29 0.2721100125125844 1105 30-31 0.41920103268282816 1105 32-33 0.282426730486101 1105 34-35 0.32968991236374023 1105 36-37 0.25334955923374025 1105 38-39 0.15328794737255436 1105 40-41 0.13253457593329898 1105 42-43 0.2313693002230366 1105 44-45 0.43103604267162865 1105 46-47 0.540812821449606 1105 48-49 0.4934425586968345 1105 50-51 0.426009173175558 1105 52-53 0.4629157969297779 1105 54-55 0.3453567353577043 1105 56-57 0.42577682704408204 1105 58-59 0.42776319894564807 1105 60-61 0.34185771143436483 1105 62-63 0.5067810449133887 1105 64-65 0.19413865336497338 1105 66-67 0.3494424975506121 1105 68-69 0.41648875450226797 1105 70-71 0.3823913947121724 1105 72-73 0.44714547124625525 1105 74-75 0.4426494731903077 1105 76-77 0.44699967553534137 1105 78-79 0.43170252364641115 1105 80-81 0.4963046314233992 1105 82-83 0.6526351652170455 1105 84-85 0.7256869699794635 1105 86-87 0.6335245329767893 1105 88-89 0.7009706118514778 1105 90-91 0.9153298639543195 1105 92-93 1.051672083779728 1105 94-95 1.077481720726574 1105 96-97 1.304953513352558 1105 98-99 1.0915242369121927 1105 100-101 1.0989779866380438 1106 1 0.30769986405681493 1106 2 0.135795493978641 1106 3 0.16189893148085588 1106 4 0.14405538674652973 1106 5 0.049191418816405985 1106 6 0.14411654024065967 1106 7 0.1933883737694444 1106 8 0.20904722045960966 1106 9 0.33007261605048654 1106 10-11 0.29999664541658433 1106 12-13 0.31253730109525435 1106 14-15 0.46736121950363696 1106 16-17 0.48385945069314573 1106 18-19 0.33514981867215 1106 20-21 0.34091647971545314 1106 22-23 0.381428072849495 1106 24-25 0.39722512652024733 1106 26-27 0.349364745140889 1106 28-29 0.37771642871212663 1106 30-31 0.4677353005804932 1106 32-33 0.4342605018459622 1106 34-35 0.2644356791849347 1106 36-37 0.3155104321510933 1106 38-39 0.3331895611204416 1106 40-41 0.5011681392709662 1106 42-43 0.34678645987158063 1106 44-45 0.46326432884033153 1106 46-47 0.34335206199889967 1106 48-49 0.2333987600855849 1106 50-51 0.12349448069927149 1106 52-53 0.18778311611048792 1106 54-55 0.31814898471279207 1106 56-57 0.2195084614487257 1106 58-59 0.029174016099482003 1106 60-61 0.06951169149527203 1106 62-63 0.1304248396454355 1106 64-65 0.2611473161071842 1106 66-67 0.18017292697823706 1106 68-69 0.2844031502196316 1106 70-71 0.3276852563604393 1106 72-73 0.32380928508336737 1106 74-75 0.17701992725086058 1106 76-77 0.2977434518411748 1106 78-79 0.4154329094330187 1106 80-81 0.4540464875244119 1106 82-83 0.5361791068738526 1106 84-85 0.6023689675743569 1106 86-87 0.5723504319817714 1106 88-89 0.7078795306209962 1106 90-91 0.9615066076554655 1106 92-93 0.7294840806957623 1106 94-95 0.7659475813274632 1106 96-97 1.0560687838298932 1106 98-99 1.1391574996117306 1106 100-101 1.280310055998168 1107 1 0.19829581751200465 1107 2 0.11577504521109461 1107 3 0.11455651762192076 1107 4 0.15609478704999447 1107 5 0.15394110982396825 1107 6 0.15704711994061427 1107 7 0.17155165240525605 1107 8 0.16530155389008172 1107 9 0.32997213070627396 1107 10-11 0.18305965223954246 1107 12-13 0.21416309116127508 1107 14-15 0.3413510277775984 1107 16-17 0.2358204849887926 1107 18-19 0.2902093185565917 1107 20-21 0.23701274969947406 1107 22-23 0.3637250673328509 1107 24-25 0.3304758525268525 1107 26-27 0.32671095232156944 1107 28-29 0.20348960063250132 1107 30-31 0.29182816913561993 1107 32-33 0.26606185236898483 1107 34-35 0.4394020165946202 1107 36-37 0.29649452280896327 1107 38-39 0.21739904291752765 1107 40-41 0.22840789479011647 1107 42-43 0.2330241755382545 1107 44-45 0.24569000736762092 1107 46-47 0.08331860037927896 1107 48-49 0.2763430560350244 1107 50-51 0.3032944771822841 1107 52-53 0.4022326573948902 1107 54-55 0.34771208699682177 1107 56-57 0.3370483666910431 1107 58-59 0.44830466714402206 1107 60-61 0.29365618439597796 1107 62-63 0.24502617660294135 1107 64-65 0.4786239783859969 1107 66-67 0.494878894300399 1107 68-69 0.355074492804313 1107 70-71 0.39188722687804045 1107 72-73 0.497334599853712 1107 74-75 0.3080091677826289 1107 76-77 0.3535517559500221 1107 78-79 0.4711831204522383 1107 80-81 0.4749882612917098 1107 82-83 0.633240726999059 1107 84-85 0.7733981635911178 1107 86-87 0.6581944335091485 1107 88-89 0.8579167101983565 1107 90-91 1.1020111822507594 1107 92-93 1.229448596808588 1107 94-95 1.3749346136937923 1107 96-97 1.443008640839249 1107 98-99 1.597456395780224 1107 100-101 1.171722452875084 1108 1 0.010993034953195036 1108 2 -0.08091349466268838 1108 3 0.03788375514343301 1108 4 0.03505706473215042 1108 5 0.06193020907812752 1108 6 0.08521514920050777 1108 7 0.04524284575006021 1108 8 0.050682362840163364 1108 9 0.14645233724672124 1108 10-11 0.13683457335256577 1108 12-13 0.1567952268468744 1108 14-15 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-1.0287343940331084 2316 70-71 -0.784621976471712 2316 72-73 -0.6289285646102449 2316 74-75 -0.669275950014601 2316 76-77 -1.0265162094220202 2316 78-79 -0.7894788589744586 2316 80-81 -0.7953195139845306 2316 82-83 -0.8896148666028694 2316 84-85 -0.7372729414702519 2316 86-87 -0.6340937575663332 2316 88-89 -0.6842967095478691 2316 90-91 -0.6761390457134802 2316 92-93 -1.1234246828620904 2316 94-95 -0.7368720735712948 2316 96-97 -0.4466556653299172 2316 98-99 -0.4391123773576773 2316 100-101 -0.36486663215934456 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 4.0 4 9.0 5 25.0 6 63.0 7 165.0 8 367.0 9 686.0 10 1236.0 11 1995.0 12 3174.0 13 4263.0 14 5618.0 15 7744.0 16 10416.0 17 14552.0 18 12989.0 19 7721.0 20 6373.0 21 6533.0 22 6802.0 23 7461.0 24 8411.0 25 10307.0 26 12492.0 27 15555.0 28 19502.0 29 24193.0 30 30852.0 31 38207.0 32 48941.0 33 63456.0 34 86633.0 35 123386.0 36 185039.0 37 274720.0 38 202593.0 39 16149.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.20152505792868 19.883046047341 15.215377084717318 21.700051810013 2 15.481792879554362 21.48766765638991 39.94609888280718 23.084440581248543 3 19.097682112628046 26.190387278593807 30.399339585238554 24.31259102353959 4 12.832901118039269 17.79686198984294 37.578180119367694 31.7920567727501 5 13.333073787131328 38.26341344041468 33.88849736256036 14.515015409893635 6 30.49561825159102 37.3174006658033 17.62575180950716 14.561229273098528 7 27.592563018967464 33.86692981013468 20.99514630602028 17.545360864877576 8 26.496603855134897 34.613266933252184 19.788294125451976 19.101835086160936 9 26.857551009706565 16.77232362412236 20.675288189647024 35.694837176524054 10-11 24.604640353717226 27.400412749551595 27.18084905641639 20.814097840314787 12-13 26.13965727651785 24.868825002542458 27.981271610902063 21.010246110037627 14-15 22.954467267265358 25.744081078439862 27.008985144994607 24.29246650930017 16-17 21.478739235345014 29.23918250991353 27.41259763568888 21.869480619052574 18-19 21.378431997254165 27.13233325361722 30.728854241069474 20.76038050805914 20-21 22.359769505342257 26.215013139625654 30.81787465810158 20.607342696930512 22-23 22.000456050333973 26.25975959632395 30.70740239609163 21.032381957250447 24-25 21.809910601736956 26.444811082953308 30.836660160665 20.908618154644735 26-27 21.72138311311685 26.62870150404017 30.741413146248643 20.908502236594337 28-29 21.298362843987494 26.55362967141671 31.082525216810943 21.065482267784855 30-31 21.509204716031313 26.52211364786786 30.921798652448963 21.046882983651866 32-33 21.35719535207071 26.705293475022344 30.800516436587543 21.136994736319394 34-35 21.824699875218645 26.600278165884912 30.535677893280756 21.039344065615687 36-37 21.968494020871827 26.35945165469358 30.493295454861464 21.178758869573127 38-39 21.70536630479734 26.644133756182942 30.622431925542877 21.02806801347684 40-41 21.724510685328564 26.560151727573682 30.67950450173073 21.03583308536702 42-43 21.607332906668955 26.709507901832236 30.2711225682812 21.412036623217613 44-45 21.771241019969395 26.96416568543793 29.903640954501178 21.360952340091497 46-47 21.91176610796484 27.145980715570555 29.333196677027125 21.609056499437486 48-49 22.17880639219087 27.29147060354139 28.958482222131295 21.571240782136442 50-51 22.202346121439135 27.390167328695753 28.785850970558872 21.62163557930624 52-53 22.32577503720353 27.568513693999048 28.669325140213424 21.436386128583994 54-55 21.99054817562151 27.485851264070277 28.737328111629413 21.786272448678805 56-57 22.100369768302897 27.928391754182734 28.39791169100273 21.57332678651164 58-59 22.230984057873137 27.5033270698347 28.57184285901566 21.6938460132765 60-61 22.215830721999403 27.475894156756308 28.83099372644238 21.47728139480191 62-63 22.241868757612426 27.492168090301146 28.85920677433374 21.40675637775269 64-65 22.10517701581203 27.387148174146425 28.785175633357053 21.722499176684497 66-67 22.05214786683166 27.754328606642524 28.453500128115238 21.740023398410578 68-69 21.965576939425553 27.720828867509432 28.616920103000638 21.69667409006438 70-71 22.073731785247368 27.626755927776998 28.47184179299786 21.827670493977774 72-73 22.28596421673355 27.5654221683366 28.271267319385395 21.877346295544452 74-75 22.107666357600213 27.986245351092336 28.08194471921432 21.824143572093128 76-77 22.168309602334617 27.783502738314393 28.19899715641166 21.84919050293933 78-79 22.117858306253623 27.773610989726926 28.138769932386804 21.96976077163265 80-81 22.072138822201097 28.22445025443771 27.94655318242801 21.756857740933185 82-83 22.230317572560203 28.016422879208015 27.914763111686703 21.83849643654508 84-85 22.14651091466428 27.944464484634295 27.715913623427003 22.193110977274426 86-87 22.15656783549484 27.79373452162741 28.003410108444942 22.0462875344328 88-89 22.29365404424924 27.645108275290287 27.928315167280367 22.132922513180105 90-91 22.088898246506123 27.80733813749871 28.05391576555461 22.049847850440557 92-93 22.10865531333039 27.758839660377166 27.929342305511977 22.20316272078047 94-95 21.857297219993164 27.674971993357854 27.950469955427724 22.517260831221247 96-97 22.02618128425905 27.54270786241001 27.963887116650206 22.467223736680733 98-99 21.84569289460794 27.675859019601535 27.741487469614068 22.73696061617646 100-101 21.822669433198623 27.901690389161743 27.68303743279818 22.592602744841457 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 957.0 1 689.0 2 449.0 3 493.5 4 439.5 5 423.0 6 730.0 7 1537.0 8 2516.5 9 2937.5 10 2525.0 11 2020.0 12 2008.0 13 2099.5 14 2280.0 15 2435.0 16 2466.5 17 2567.5 18 2649.0 19 2773.0 20 3018.0 21 3492.5 22 4062.0 23 4714.0 24 5532.0 25 6660.0 26 8262.0 27 9981.5 28 11341.5 29 13068.0 30 15437.5 31 17732.0 32 20403.0 33 23267.0 34 26960.0 35 29655.5 36 32849.5 37 36368.0 38 37478.0 39 37992.5 40 38453.0 41 39968.5 42 41054.5 43 42064.5 44 43781.0 45 44704.0 46 44394.5 47 44968.0 48 50291.0 49 50538.0 50 45138.0 51 42429.5 52 40235.5 53 39082.0 54 37868.0 55 35224.0 56 31802.0 57 27977.5 58 25051.0 59 22913.5 60 20182.5 61 16498.5 62 13080.5 63 10247.5 64 7607.5 65 5700.5 66 4133.5 67 3199.5 68 2302.5 69 1408.0 70 972.0 71 697.5 72 534.0 73 431.5 74 325.0 75 232.0 76 146.0 77 78.0 78 43.0 79 22.5 80 18.5 81 13.0 82 10.5 83 9.5 84 4.0 85 1.0 86 1.5 87 0.5 88 1.0 89 1.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.015016291484491509 2 0.0025424408862631124 3 0.002383538330871668 4 7.945127769572226E-5 5 0.002065733220088779 6 2.383538330871668E-4 7 3.1780511078288904E-4 8 0.0 9 0.0 10-11 3.972563884786113E-5 12-13 7.150614992615003E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0028205203581981404 22-23 0.0 24-25 0.004091740801329696 26-27 0.001827379387001612 28-29 0.003813661329394669 30-31 0.016049158094535897 32-33 6.356102215657781E-4 34-35 0.002502715247415251 36-37 0.0036944844128510854 38-39 0.0023438126920238066 40-41 0.004886253578286919 42-43 0.0025821665251109737 44-45 5.561589438700559E-4 46-47 7.945127769572226E-5 48-49 2.7807947193502793E-4 50-51 1.9862819423930567E-4 52-53 0.0012712204431315562 54-55 0.0031780511078288907 56-57 5.561589438700559E-4 58-59 0.0014301229985230006 60-61 0.0013506717208272784 62-63 0.0 64-65 1.9862819423930567E-4 66-67 3.972563884786113E-5 68-69 0.0 70-71 2.7807947193502793E-4 72-73 0.0 74-75 3.1780511078288904E-4 76-77 9.534153323486672E-4 78-79 2.383538330871668E-4 80-81 0.005839668910635586 82-83 0.001827379387001612 84-85 0.004052015162481836 86-87 0.0017479281093058898 88-89 9.931409711965284E-4 90-91 2.383538330871668E-4 92-93 1.191769165435834E-4 94-95 2.383538330871668E-4 96-97 0.0019068306646973345 98-99 0.0025821665251109737 100-101 0.0013903973596751397 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1258633.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.745156540027075 #Duplication Level Percentage of deduplicated Percentage of total 1 78.92425509658368 45.57493465357255 2 12.437292631540362 14.36386819884847 3 3.667042861202008 6.352618921772961 4 1.6322624866592153 3.7702101122620095 5 0.8332196663208465 2.4057200031963206 6 0.5025007601087204 1.7410191032376392 7 0.34570834089493113 1.3974087583519588 8 0.22774964057679067 1.0521150917633344 9 0.18688007258305547 0.9712277140968143 >10 1.1030030237210182 11.942021047866543 >50 0.09665979460995626 3.777549045881395 >100 0.04094416978189393 4.0774640359923575 >500 0.0013785863431255343 0.499452336593802 >1k 8.271518058753206E-4 0.8839526212205258 >5k 2.7571726862510683E-4 1.1904383553432765 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8408 0.6680263428656328 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6546 0.5200880637961979 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4642 0.3688128310635428 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1646 0.13077680308715883 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1310 0.10408117378139616 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 7.945127769572226E-5 0.0 5 0.0 0.0 0.0 7.945127769572226E-5 0.0 6 0.0 0.0 0.0 7.945127769572226E-5 0.0 7 0.0 0.0 0.0 7.945127769572226E-5 0.0 8 0.0 0.0 0.0 7.945127769572226E-5 0.0 9 0.0 0.0 0.0 7.945127769572226E-5 0.0 10-11 0.0 0.0 0.0 7.945127769572226E-5 0.0 12-13 0.0 0.0 0.0 7.945127769572226E-5 0.0 14-15 0.0 0.0 0.0 7.945127769572226E-5 0.0 16-17 0.0 0.0 0.0 1.5890255539144452E-4 0.0 18-19 0.0 0.0 0.0 1.5890255539144452E-4 0.0 20-21 0.0 0.0 0.0 1.9862819423930564E-4 0.0 22-23 0.0 0.0 0.0 2.7807947193502793E-4 0.0 24-25 0.0 0.0 0.0 5.95884582717917E-4 0.0 26-27 0.0 0.0 0.0 8.739640546529449E-4 0.0 28-29 0.0 0.0 0.0 0.0015095742762187229 0.0 30-31 0.0 0.0 0.0 0.003615033135155363 0.0 32-33 0.0 0.0 0.0 0.005720491994092003 0.0 34-35 0.0 0.0 0.0 0.007587597019941476 0.0 36-37 0.0 0.0 0.0 0.010408117378139617 0.0 38-39 0.0 0.0 0.0 0.012871106986707006 0.0 40-41 0.0 0.0 0.0 0.01581080426144873 0.0 42-43 0.0 0.0 0.0 0.02041897836780062 0.0 44-45 0.0 0.0 0.0 0.027649044638111346 0.0 46-47 0.0 0.0 0.0 0.04330094634416863 0.0 48-49 0.0 0.0 0.0 0.07742527011448135 0.0 50-51 0.0 0.0 0.0 0.10153873289513304 0.0 52-53 0.0 0.0 0.0 0.12473850598228396 0.0 54-55 0.0 0.0 0.0 0.15560532736707205 0.0 56-57 0.0 0.0 0.0 0.1791626312038537 0.0 58-59 0.0 0.0 0.0 0.20816234756279234 0.0 60-61 0.0 0.0 0.0 0.2556742116248343 0.0 62-63 0.0 0.0 0.0 0.28618350225999156 0.0 64-65 0.0 0.0 0.0 0.3203475516691522 0.0 66-67 0.0 0.0 0.0 0.38819894282129896 0.0 68-69 0.0 0.0 0.0 0.463161223327213 0.0 70-71 0.0 0.0 0.0 0.5285496248707924 0.0 72-73 0.0 0.0 0.0 0.6072461154284052 0.0 74-75 0.0 0.0 0.0 0.6772426910783366 0.0 76-77 0.0 0.0 0.0 0.7329777623818857 0.0 78-79 0.0 0.0 0.0 0.802140099616012 0.0 80-81 0.0 0.0 0.0 0.9186553983567887 0.0 82-83 0.0 0.0 0.0 1.0321912741839756 0.0 84-85 0.0 0.0 0.0 1.1623721926884167 0.0 86-87 0.0 0.0 0.0 1.4056917306315662 0.0 88-89 0.0 0.0 0.0 1.6967217608309968 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 9285 0.0 31.982428 1 GGTATCA 6085 0.0 28.89046 1 ATCAACG 11450 0.0 25.761944 3 TCAACGC 11525 0.0 25.63551 4 TATCAAC 11550 0.0 25.540926 2 CAACGCA 11675 0.0 25.265461 5 AACGCAG 11895 0.0 24.757256 6 GTACATG 6470 0.0 23.132341 1 GAGTACT 6780 0.0 22.417933 12-13 TACATGG 6725 0.0 22.392057 2 AGTACTT 7055 0.0 22.38566 12-13 ACGCAGA 13170 0.0 22.324419 7 CGCAGAG 13110 0.0 22.24544 8 ACATGGG 6570 0.0 22.050934 3 GCAGAGT 13575 0.0 21.238514 9 GTACTTT 7155 0.0 21.209797 14-15 GACAACG 115 0.0037243837 20.6513 7 AGAGTAC 11980 0.0 19.585985 10-11 TACTTTT 7975 0.0 19.565004 14-15 CATTCCG 170 7.957201E-5 19.557999 9 >>END_MODULE