FastQCFastQC Report
Thu 26 May 2016
SRR936970_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936970_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1115950
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71150.6375733679824365No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51810.4642681123706259No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35410.31730812312379586No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11300.10125901698104754No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48250.033.2884181
GTATCAA73600.032.7343671
TATCAAC90200.026.6047172
ATCAACG91050.026.4085393
TCAACGC91300.026.2841784
CAACGCA92500.025.9980645
GTACATG57650.025.9648021
AACGCAG94100.025.706386
TACATGG58950.024.7473322
CGTCCGA500.001649065523.7625210-11
GAGTACT52850.023.55913412-13
ACATGGG58950.023.5381773
ACGCAGA103100.023.4173247
CGCAGAG104050.023.1588828
GTATAGG3500.023.0810011
GCAGAGT106800.021.7615229
GTACTTT56700.021.6222414-15
AGTACTT55450.020.82608812-13
CATGGGA39450.020.2365284
TAGGACA5200.020.1044944
Produced by FastQC (version 0.11.5)