##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936970_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1115950 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.772318652269366 33.0 31.0 34.0 30.0 34.0 2 31.89000224024374 33.0 31.0 34.0 30.0 34.0 3 31.894276625296833 33.0 31.0 34.0 30.0 34.0 4 35.493596487297815 37.0 35.0 37.0 33.0 37.0 5 35.49924010932389 37.0 35.0 37.0 33.0 37.0 6 35.562932031004976 37.0 35.0 37.0 33.0 37.0 7 35.502506384694655 37.0 35.0 37.0 33.0 37.0 8 35.44886240422958 37.0 35.0 37.0 33.0 37.0 9 37.088047851606255 39.0 37.0 39.0 33.0 39.0 10-11 36.91118016040145 39.0 37.0 39.0 33.0 39.0 12-13 36.84504682109414 39.0 37.0 39.0 33.0 39.0 14-15 37.9802235763251 40.0 38.0 41.0 33.0 41.0 16-17 37.91819391549801 40.0 38.0 41.0 33.0 41.0 18-19 37.90984900757202 40.0 38.0 41.0 33.0 41.0 20-21 37.84503382768045 40.0 38.0 41.0 32.5 41.0 22-23 37.734922711591025 40.0 37.0 41.0 32.0 41.0 24-25 37.58446301357588 40.0 37.0 41.0 32.0 41.0 26-27 37.66151037232851 40.0 37.0 41.0 32.0 41.0 28-29 37.60622698149558 40.0 37.0 41.0 32.0 41.0 30-31 37.43423630090954 40.0 37.0 41.0 32.0 41.0 32-33 37.2198346700121 40.0 36.0 41.0 31.5 41.0 34-35 37.04210986155294 39.5 36.0 41.0 31.0 41.0 36-37 36.84892557910301 39.0 36.0 41.0 30.5 41.0 38-39 36.682302074465696 39.0 35.0 41.0 30.0 41.0 40-41 36.49595546395447 39.0 35.0 41.0 30.0 41.0 42-43 36.26455979210538 39.0 35.0 41.0 30.0 41.0 44-45 35.988289797930015 39.0 35.0 40.5 29.5 41.0 46-47 35.59435368968144 39.0 35.0 40.0 27.5 41.0 48-49 35.243614409247726 38.5 35.0 40.0 26.0 41.0 50-51 34.573509117792014 38.0 34.0 39.5 25.0 40.5 52-53 34.8020870110668 38.0 34.5 40.0 26.0 40.5 54-55 35.181942739370044 39.0 35.0 40.5 26.0 41.0 56-57 35.07716116313455 39.0 35.0 41.0 26.0 41.0 58-59 34.84060755410189 38.0 34.5 40.0 26.0 41.0 60-61 34.5649159908598 38.0 34.0 40.0 25.0 41.0 62-63 34.24335454097405 38.0 34.0 40.0 23.5 41.0 64-65 33.92745239482056 37.0 34.0 40.0 23.0 41.0 66-67 33.55175366279852 37.0 33.0 39.5 22.0 41.0 68-69 33.18059993727317 36.0 33.0 39.0 22.0 41.0 70-71 32.76587974371611 36.0 32.5 39.0 20.5 40.5 72-73 32.37206102423944 35.0 32.0 38.5 20.0 40.0 74-75 31.936174559792107 35.0 32.0 37.5 18.0 39.5 76-77 31.495706348850753 35.0 31.5 37.0 16.5 39.0 78-79 31.05755768627627 35.0 31.0 36.5 12.0 39.0 80-81 30.623290918051886 35.0 31.0 36.0 7.5 37.5 82-83 30.213979568977102 34.0 30.5 35.5 7.0 37.0 84-85 29.852244724225997 34.0 30.0 35.0 2.0 37.0 86-87 29.530631748734265 34.0 30.0 35.0 2.0 36.0 88-89 29.166692056095705 34.0 29.5 35.0 2.0 36.0 90-91 28.8052296249832 34.0 29.0 35.0 2.0 36.0 92-93 28.433587974371612 34.0 29.0 35.0 2.0 35.0 94-95 28.027904924055736 34.0 29.0 35.0 2.0 35.0 96-97 27.554771271114298 34.0 27.0 35.0 2.0 35.0 98-99 26.952155562525203 34.0 26.5 35.0 2.0 35.0 100-101 24.763134997087683 31.5 21.0 34.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.32446062962717903 1101 2 0.3354029758177255 1101 3 0.27294882714256374 1101 4 0.20718492530369303 1101 5 0.26886630333939365 1101 6 0.14422395944769306 1101 7 0.1995881139817186 1101 8 0.23270512439182767 1101 9 0.18728618800131613 1101 10-11 0.28548454040343785 1101 12-13 0.3428131593016488 1101 14-15 0.43018746161076393 1101 16-17 0.5329689652320369 1101 18-19 0.3075404570441762 1101 20-21 0.3929491951768824 1101 22-23 0.4618851288522663 1101 24-25 0.5367590228750743 1101 26-27 0.5823933095335008 1101 28-29 0.5044683853208127 1101 30-31 0.412877530707803 1101 32-33 0.3677639239998314 1101 34-35 0.2743595034702224 1101 36-37 0.3978449494774381 1101 38-39 0.30716792631292833 1101 40-41 0.461699949587036 1101 42-43 0.3509647464164871 1101 44-45 0.41601984781434 1101 46-47 0.30120772292922027 1101 48-49 0.4098850602991746 1101 50-51 0.26300415040679326 1101 52-53 0.16491923685209287 1101 54-55 0.2713000607057907 1101 56-57 0.32757617320664423 1101 58-59 0.19800916567728422 1101 60-61 0.327097171720375 1101 62-63 0.2047857517870284 1101 64-65 0.27126896317135873 1101 66-67 0.3506444614941344 1101 68-69 0.37086429260706666 1101 70-71 0.21633049389598114 1101 72-73 0.2913487976384701 1101 74-75 0.091692483065831 1101 76-77 0.22003008196428908 1101 78-79 0.17876713300001157 1101 80-81 0.016120720353033136 1101 82-83 0.16247145262008544 1101 84-85 0.33968383796347723 1101 86-87 0.2173438539138388 1101 88-89 0.32816327731363515 1101 90-91 0.3567682874428293 1101 92-93 0.3365406858406921 1101 94-95 0.21997343664275704 1101 96-97 0.32715187772147303 1101 98-99 0.5748059309988349 1101 100-101 0.825500830135649 1102 1 0.39620497544957445 1102 2 0.37409088781251043 1102 3 0.3047572963530456 1102 4 0.3813087774688171 1102 5 0.22528741926390694 1102 6 0.2386011575098621 1102 7 0.2345946816855644 1102 8 0.2518382278387108 1102 9 0.3243151242350635 1102 10-11 0.37490165920926444 1102 12-13 0.4674007861914973 1102 14-15 0.5707928202921337 1102 16-17 0.722237377502239 1102 18-19 0.4512384879436553 1102 20-21 0.4717702166717359 1102 22-23 0.4023349207236464 1102 24-25 0.5214817971700327 1102 26-27 0.4391406920361689 1102 28-29 0.4117593436809628 1102 30-31 0.42088387444386655 1102 32-33 0.6249904159581732 1102 34-35 0.5895598537477582 1102 36-37 0.4527685078613999 1102 38-39 0.5805412846391818 1102 40-41 0.5076647967340691 1102 42-43 0.5769225113390988 1102 44-45 0.8318553787894842 1102 46-47 1.001524707959696 1102 48-49 0.898754769564782 1102 50-51 0.772322017184301 1102 52-53 0.6432992839458578 1102 54-55 0.7049707917487424 1102 56-57 0.8250656154461637 1102 58-59 0.8128082907905352 1102 60-61 0.7709251442873466 1102 62-63 0.7897083820162223 1102 64-65 0.9404508956299438 1102 66-67 0.6848956928124963 1102 68-69 0.4516354479781626 1102 70-71 0.7748082797222509 1102 72-73 0.7066012052921096 1102 74-75 0.7023754435567007 1102 76-77 0.78562540245008 1102 78-79 0.7931097472084438 1102 80-81 0.5934506410361529 1102 82-83 0.5647276757969202 1102 84-85 0.7280984487337037 1102 86-87 0.7198967839571999 1102 88-89 0.7213477964991988 1102 90-91 0.927576407586411 1102 92-93 1.042532025753161 1102 94-95 0.9622379728579418 1102 96-97 0.9653475650301679 1102 98-99 1.0206753396230042 1102 100-101 0.9445165018271311 1103 1 0.19526088266038144 1103 2 0.2764803570662586 1103 3 0.27782469161343215 1103 4 0.2260024926311388 1103 5 0.1759583406120342 1103 6 0.16831186716164837 1103 7 0.09631360849814996 1103 8 0.2985292947485618 1103 9 0.1273080796377144 1103 10-11 0.36786781462460993 1103 12-13 0.2871245322910667 1103 14-15 0.43609216191568123 1103 16-17 0.3865188930891392 1103 18-19 0.3875391065860825 1103 20-21 0.44608083640991936 1103 22-23 0.4763456919222193 1103 24-25 0.4538568884405194 1103 26-27 0.44332633391992715 1103 28-29 0.4929201917361965 1103 30-31 0.5055864785587971 1103 32-33 0.41243743298748115 1103 34-35 0.48153398976307216 1103 36-37 0.343648934039571 1103 38-39 0.3921259554970362 1103 40-41 0.3879560533022115 1103 42-43 0.4640389576423445 1103 44-45 0.40309987311766093 1103 46-47 0.4259225275037224 1103 48-49 0.5876071218089791 1103 50-51 0.478985044978657 1103 52-53 0.5383617677527042 1103 54-55 0.517090540687029 1103 56-57 0.4151320572946986 1103 58-59 0.5914295917112824 1103 60-61 0.4879420609472689 1103 62-63 0.589554361454887 1103 64-65 0.5626995958254355 1103 66-67 0.6555334751621942 1103 68-69 0.5916446441526304 1103 70-71 0.5467147347608403 1103 72-73 0.4191820942488178 1103 74-75 0.3443383147703223 1103 76-77 0.35709980114008744 1103 78-79 0.3937991530720808 1103 80-81 0.41282133800991616 1103 82-83 0.5346434867653578 1103 84-85 0.5150070594457112 1103 86-87 0.609044222802126 1103 88-89 0.7834240223400393 1103 90-91 0.7941176935966503 1103 92-93 0.6287336165479722 1103 94-95 0.8293747572486119 1103 96-97 1.0229078854830504 1103 98-99 1.2086469891893614 1103 100-101 1.2969688491557854 1104 1 0.4043611175627042 1104 2 0.196035869200049 1104 3 0.23318794065662019 1104 4 0.24561821706905107 1104 5 0.18232470327913575 1104 6 0.29361027381877136 1104 7 0.30960481091769054 1104 8 0.2907133766118122 1104 9 0.16886306504658677 1104 10-11 0.4372038226444275 1104 12-13 0.4427672322660001 1104 14-15 0.4259949195754942 1104 16-17 0.595528597146199 1104 18-19 0.6042495077040968 1104 20-21 0.48419910102123254 1104 22-23 0.4846697509323974 1104 24-25 0.6403119341678902 1104 26-27 0.4760021283612801 1104 28-29 0.4084599573436094 1104 30-31 0.6104825247236931 1104 32-33 0.6130890957815964 1104 34-35 0.5084864294764344 1104 36-37 0.511267988738247 1104 38-39 0.5639298715268879 1104 40-41 0.5715032270407292 1104 42-43 0.4049682448221148 1104 44-45 0.5466958644861606 1104 46-47 0.5592423387323038 1104 48-49 0.6836468088324423 1104 50-51 0.5938686876212884 1104 52-53 0.7608701252629899 1104 54-55 0.723062549930205 1104 56-57 0.7455231824046038 1104 58-59 0.9377650305283609 1104 60-61 0.9697440336170402 1104 62-63 0.8065371515677526 1104 64-65 0.7903129903551402 1104 66-67 0.7868780679486136 1104 68-69 0.7896236558637639 1104 70-71 0.8810939958167552 1104 72-73 0.9452812608340722 1104 74-75 0.8992618653210478 1104 76-77 0.9435142805532166 1104 78-79 1.133964211245452 1104 80-81 1.0499487544666728 1104 82-83 1.051877267673273 1104 84-85 0.9055462409831527 1104 86-87 0.874697828956311 1104 88-89 1.02941889526765 1104 90-91 1.1105235287742339 1104 92-93 0.807237877072918 1104 94-95 0.8627620551073072 1104 96-97 0.8621322724219702 1104 98-99 0.9271698656171488 1104 100-101 0.9706757713406731 1105 1 0.2809459115785593 1105 2 0.1947363269425928 1105 3 0.2169738064599045 1105 4 0.2632117193577628 1105 5 0.27039548590724394 1105 6 0.31002257704530223 1105 7 0.22820757970188055 1105 8 0.2614089219953115 1105 9 0.29604069148400924 1105 10-11 0.3577983297338463 1105 12-13 0.35896378245021054 1105 14-15 0.49971296133053755 1105 16-17 0.3386314775749142 1105 18-19 0.36985824947031887 1105 20-21 0.575504504616184 1105 22-23 0.3903407345206489 1105 24-25 0.44718281727607945 1105 26-27 0.30089801984280484 1105 28-29 0.2953143728845262 1105 30-31 0.08375901238696315 1105 32-33 0.14246249089156038 1105 34-35 0.31442675547843635 1105 36-37 0.2716972619323954 1105 38-39 0.1742421970823287 1105 40-41 0.02883213180795252 1105 42-43 0.25750927418462055 1105 44-45 0.4965309097021944 1105 46-47 0.41631166998160296 1105 48-49 0.4024438390881002 1105 50-51 0.31336042592652547 1105 52-53 0.07984411208894215 1105 54-55 0.18482625429550836 1105 56-57 0.4362703633803804 1105 58-59 0.2956761614873855 1105 60-61 0.25410765983117756 1105 62-63 0.26013015145610296 1105 64-65 0.3701798542604706 1105 66-67 0.41861302384902643 1105 68-69 0.41733996712869725 1105 70-71 0.6008425165268108 1105 72-73 0.5251453336262983 1105 74-75 0.6612081084828212 1105 76-77 0.6280082517489234 1105 78-79 0.47923522832705956 1105 80-81 0.7125718425542011 1105 82-83 0.7872970510828985 1105 84-85 0.5295327878180309 1105 86-87 0.6203029422755044 1105 88-89 0.5318600027813325 1105 90-91 0.6604975714224395 1105 92-93 0.6090367494632254 1105 94-95 0.8537649020426663 1105 96-97 0.8879871903632477 1105 98-99 0.8928369416470687 1105 100-101 0.8081132504073274 1106 1 0.23188930780496975 1106 2 0.10266085524447988 1106 3 0.10904927815801457 1106 4 0.04736266275399004 1106 5 0.012282278360075338 1106 6 0.05568295440379245 1106 7 0.1414151268716921 1106 8 0.31574854463681845 1106 9 0.3239652197858973 1106 10-11 0.24081719765838017 1106 12-13 0.14764302773323124 1106 14-15 0.17933560283997707 1106 16-17 0.18693336436736274 1106 18-19 0.17740541928163367 1106 20-21 0.2570139385784529 1106 22-23 0.14166148923763444 1106 24-25 0.3441639493515467 1106 26-27 0.2771244349371429 1106 28-29 0.22965399552603571 1106 30-31 0.3350797671039487 1106 32-33 0.4360473965519347 1106 34-35 0.5457475101954259 1106 36-37 0.35698028080032174 1106 38-39 0.42669502727100195 1106 40-41 0.43524722614757394 1106 42-43 0.33901870814688806 1106 44-45 0.4003044946078589 1106 46-47 0.35329280205707647 1106 48-49 0.367726857956022 1106 50-51 0.22505853913407492 1106 52-53 0.4424856215228985 1106 54-55 0.436524367503047 1106 56-57 0.35136470300302136 1106 58-59 0.46209125582700494 1106 60-61 0.46203218813306535 1106 62-63 0.3906961891919565 1106 64-65 0.5267836278453757 1106 66-67 0.5789903823395903 1106 68-69 0.33583053316643685 1106 70-71 0.31103119577209526 1106 72-73 0.35231514494704896 1106 74-75 0.2774345235771598 1106 76-77 0.3570648555225091 1106 78-79 0.2562163604025365 1106 80-81 0.303892597271183 1106 82-83 0.2767310133470495 1106 84-85 0.2963252406482191 1106 86-87 0.26105765925663604 1106 88-89 0.1058222669322717 1106 90-91 0.23861077896961191 1106 92-93 0.2746971268217173 1106 94-95 0.2948969775143624 1106 96-97 0.25930794508022714 1106 98-99 0.44566713032631355 1106 100-101 0.5752830617280829 1107 1 0.08254326800382827 1107 2 0.14244370446335708 1107 3 0.22677922637817716 1107 4 0.30442375116327014 1107 5 0.33387963478534743 1107 6 0.1399740426655498 1107 7 0.14242712032176286 1107 8 0.37580666787578565 1107 9 0.38512738952345416 1107 10-11 0.45863270800696654 1107 12-13 0.25431303820722206 1107 14-15 0.13655807912599016 1107 16-17 0.2605772783921765 1107 18-19 0.306838496420319 1107 20-21 0.4469597984040803 1107 22-23 0.2730613151629555 1107 24-25 0.4847965190182961 1107 26-27 0.13774756326558446 1107 28-29 0.3601147033313694 1107 30-31 0.36188461169066954 1107 32-33 0.3943314333141501 1107 34-35 0.2112223062477625 1107 36-37 0.09294410867292413 1107 38-39 -0.024609795187579664 1107 40-41 0.3647340008978901 1107 42-43 0.2139779186149724 1107 44-45 0.33471876283038426 1107 46-47 0.28146546053141464 1107 48-49 0.3974319636547605 1107 50-51 0.29894320728578094 1107 52-53 0.2624558222988611 1107 54-55 0.3981152330082338 1107 56-57 0.4783011362424361 1107 58-59 0.4277153430413634 1107 60-61 0.3616176543729068 1107 62-63 0.4524926988472018 1107 64-65 0.5156372428453011 1107 66-67 0.30596310572313 1107 68-69 0.3967345413095842 1107 70-71 0.23242969400921965 1107 72-73 0.30997162775349096 1107 74-75 0.26047433947106313 1107 76-77 0.11338597904176595 1107 78-79 0.291721693283467 1107 80-81 0.33849067654892195 1107 82-83 0.2114052134149098 1107 84-85 0.3627639383336181 1107 86-87 0.2672977937957093 1107 88-89 0.3387206102724427 1107 90-91 0.28391533589055484 1107 92-93 0.2844479640028865 1107 94-95 0.2243654596717093 1107 96-97 0.16692238442700713 1107 98-99 0.09863333328558355 1107 100-101 0.1401409253302468 1108 1 0.1146596564646778 1108 2 0.2538765846210431 1108 3 0.15212859671452605 1108 4 0.025527161746481397 1108 5 0.055316411595711656 1108 6 0.06927762986327934 1108 7 0.06434667721668319 1108 8 -0.1094752448240115 1108 9 -0.07711556424047927 1108 10-11 -0.023875053669705437 1108 12-13 0.09871862155383582 1108 14-15 0.19899213334007015 1108 16-17 0.22027127729403162 1108 18-19 0.23079462365361536 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2316 56-57 -1.988174474023019 2316 58-59 -1.7958532544380645 2316 60-61 -2.0704234713601366 2316 62-63 -1.925575848437795 2316 64-65 -2.427926987351526 2316 66-67 -2.4566252566411535 2316 68-69 -2.3849790015898016 2316 70-71 -2.370197783379467 2316 72-73 -2.588938236956004 2316 74-75 -2.816297505918339 2316 76-77 -3.0102315671755875 2316 78-79 -3.1705771534168505 2316 80-81 -3.2392649065749026 2316 82-83 -2.945679200442271 2316 84-85 -3.1466971639069605 2316 86-87 -3.6161858908132096 2316 88-89 -3.5592227366927744 2316 90-91 -3.176680197317282 2316 92-93 -3.3129398734081654 2316 94-95 -4.1573896310336735 2316 96-97 -4.010721232122652 2316 98-99 -3.7989216479446277 2316 100-101 -3.7968507643757903 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2258.0 3 799.0 4 810.0 5 866.0 6 973.0 7 1200.0 8 1436.0 9 1806.0 10 2371.0 11 2994.0 12 3979.0 13 4918.0 14 5677.0 15 6823.0 16 9014.0 17 11890.0 18 10433.0 19 6554.0 20 6348.0 21 6607.0 22 7117.0 23 7686.0 24 8737.0 25 10036.0 26 11825.0 27 13768.0 28 16415.0 29 19828.0 30 24415.0 31 30754.0 32 39082.0 33 51120.0 34 70009.0 35 101045.0 36 151406.0 37 223567.0 38 204357.0 39 37004.0 40 23.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.98856843698118 19.124829225787714 14.689002678600243 22.197599658630864 2 14.958597004569254 20.504097269473124 41.06762982306573 23.46967590289189 3 18.705184282231464 26.103452602896887 30.103255413738705 25.088107701132937 4 12.19615183936054 17.048309100786952 38.35941268125102 32.39612637860149 5 13.201710457768929 37.7308548612562 34.24591820540019 14.82151647557469 6 30.128595970862822 36.94314232530782 18.264716536534454 14.663545167294911 7 27.35323074384849 33.64955427194164 21.395843458082194 17.60137152612768 8 26.084285979446335 34.24621175778563 20.30160675808602 19.367895504682007 9 26.746506986027946 16.661107255390387 20.830576044676146 35.76180971390552 10-11 24.244498935364593 27.08514079237211 27.705135672078846 20.965224600184456 12-13 25.83757307929083 24.664986461359387 28.114728041239612 21.382712418110167 14-15 22.815540074622916 25.448100666513035 27.201452286436513 24.53490697242754 16-17 21.328680576818677 29.018729526698284 27.50311680591613 22.149473090566914 18-19 21.243787160490285 27.158401408602227 30.602672327851447 20.995139103056033 20-21 22.32845233676366 26.16044707202967 30.510218353766454 21.000882237440223 22-23 21.88290056376496 26.20223023069142 30.422243326495607 21.492625879048013 24-25 21.583636822347906 26.45021592644027 30.60585324888967 21.360294002322156 26-27 21.45146286248931 26.602534803813498 30.504935357304813 21.441066976392374 28-29 21.099422196370778 26.571797052636864 30.87328381925779 21.455496931734565 30-31 21.28691353510477 26.503539558906162 30.749576319414384 21.45997058657468 32-33 21.207427219246714 26.618145417672423 30.479084229010855 21.695343134070008 34-35 21.653955228194256 26.557668378203868 30.32019740854239 21.468178985059485 36-37 21.697413204432664 26.36007054408199 30.279604408288215 21.66291184319714 38-39 21.428632239846326 26.613222304321 30.417268404900415 21.54087705093226 40-41 21.54071987009216 26.436953116318417 30.497200385708705 21.525126627880717 42-43 21.419133233832763 26.613654459401392 30.092171700765252 21.875040606000592 44-45 21.453083169980395 26.848143377205265 29.85055166619994 21.848221786614396 46-47 21.62347077399536 26.988217835623214 29.297111631258044 22.09119975912338 48-49 21.765074602924976 27.127990132099217 28.990005300457057 22.11692996451875 50-51 21.831193859248607 27.259867576493907 28.86002601512067 22.04891254913681 52-53 21.943456944348608 27.35432519067688 28.756654983945594 21.945562881028916 54-55 21.606996111578606 27.310671337432353 28.8282221069168 22.254110444072243 56-57 21.7049875908503 27.73653345092672 28.566319712398126 21.99215924582485 58-59 21.84113339672201 27.25217252593723 28.73984982081198 22.16684425652878 60-61 21.870594319321494 27.23529617377416 28.973369410807287 21.920740096097056 62-63 21.889815576598714 27.19792579687077 29.030777862022116 21.8814807645084 64-65 21.734429414600832 27.227755602435444 28.97538566873444 22.06242931422928 66-67 21.591955529565176 27.601827392515222 28.527757052691012 22.27846002522859 68-69 21.5243754873753 27.51306389882313 28.880089990767882 22.082470623033693 70-71 21.685477094746748 27.4622391019393 28.62420596242396 22.228077840889995 72-73 21.75976547095812 27.323198012490053 28.525777760251238 22.39125875630059 74-75 21.630999412068373 27.666977959733853 28.390196598600436 22.31182602959734 76-77 21.75490944324164 27.530213595905284 28.428767529084244 22.286109431768832 78-79 21.72163073362216 27.5364014245926 28.412473880526772 22.329493961258468 80-81 21.558761337835453 27.909428184969293 28.25215148845085 22.279658988744405 82-83 21.76028841258345 27.78597448602755 28.198210149656155 22.255526951732843 84-85 21.639905533123905 27.654236893169077 27.97259205815001 22.73326551555701 86-87 21.696737911189405 27.592348021867657 28.288316468907958 22.422597598034976 88-89 21.881406813589535 27.302262906165375 28.279358694624406 22.536971585620684 90-91 21.63961431067466 27.453499538658782 28.477860774882963 22.429025375783596 92-93 21.6656298628192 27.450905694473327 28.376902998185745 22.50656144452173 94-95 21.461317769079848 27.345639896784046 28.366461447730117 22.826580886405985 96-97 21.512376569341463 27.431131844179873 28.264976507219945 22.79151507925872 98-99 21.43110098208154 27.421000442896005 28.067099640841825 23.08079893418063 100-101 21.479459694770313 27.348906105624838 28.26582357363786 22.905810625966986 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 475.0 1 348.0 2 286.5 3 368.5 4 323.5 5 332.5 6 621.0 7 1308.5 8 2258.0 9 2652.5 10 2157.0 11 1661.0 12 1629.5 13 1751.5 14 1867.0 15 1957.5 16 2047.5 17 2130.5 18 2177.0 19 2246.5 20 2488.0 21 2880.5 22 3366.5 23 4046.0 24 4776.5 25 5751.0 26 7263.5 27 8829.5 28 10027.0 29 11478.5 30 13749.5 31 15937.5 32 18249.5 33 20907.0 34 23992.0 35 26462.5 36 29230.5 37 32501.5 38 33775.5 39 34005.0 40 34176.5 41 35318.0 42 36457.5 43 37462.5 44 38736.0 45 39604.5 46 39599.5 47 39995.0 48 44530.5 49 44892.5 50 40339.5 51 37875.5 52 35830.0 53 35037.0 54 34028.0 55 31350.0 56 28301.5 57 24994.0 58 22287.0 59 20074.0 60 17448.5 61 14555.0 62 11511.5 63 8811.0 64 6665.5 65 5012.0 66 3712.5 67 2930.5 68 2062.0 69 1222.0 70 866.0 71 633.5 72 423.5 73 339.0 74 261.5 75 185.5 76 133.0 77 71.5 78 37.5 79 20.0 80 15.0 81 10.0 82 10.5 83 11.5 84 7.5 85 3.5 86 2.5 87 2.5 88 3.5 89 3.5 90 1.5 91 1.0 92 0.5 93 0.5 94 1.0 95 2.0 96 1.5 97 1.0 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03960750929701151 2 0.040413997042878264 3 0.02419463237600251 4 0.04552175276670102 5 0.062099556431739776 6 0.060396971190465526 7 0.06236838568036202 8 0.06478784891796227 9 0.06487745866750302 10-11 0.06985079976701465 12-13 0.0724494825036964 14-15 0.06658004390877728 16-17 0.05605089833773915 18-19 0.04641785026210852 20-21 0.05412428872261302 22-23 0.02916797347551414 24-25 0.01581612079394238 26-27 0.011111608943053004 28-29 0.015502486670549757 30-31 0.011918096688919754 32-33 0.007303194587571128 34-35 0.008199292082978628 36-37 0.004659706976119002 38-39 0.007661633585734127 40-41 0.007347999462341504 42-43 0.0038980241050226264 44-45 0.0011201218692593754 46-47 0.002329853488059501 48-49 7.616828710963753E-4 50-51 0.005421389847215377 52-53 0.005107755723822752 54-55 0.006541511716474752 56-57 0.021595949639320756 58-59 0.0074376092118822535 60-61 0.018190779156772256 62-63 0.013262242932031007 64-65 0.008602535955912004 66-67 0.01366548680496438 68-69 0.02509072987141001 70-71 0.019042071777409384 72-73 0.016308974416416506 74-75 0.015592096420090506 76-77 0.024060217751691385 78-79 0.03611272906492226 80-81 0.02930238809982526 82-83 0.02921277835028451 84-85 0.019266096151261257 86-87 0.021371925265468883 88-89 0.017429096285675884 90-91 0.01626416954164613 92-93 0.03064653434293651 94-95 0.020699852143913258 96-97 0.03535104619382589 98-99 0.050539898740983025 100-101 0.061965141807428646 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1115950.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.90550744308303 #Duplication Level Percentage of deduplicated Percentage of total 1 81.03138213705728 49.35257447871847 2 11.349484118384996 13.824921788949002 3 3.2531158937723412 5.943980228440891 4 1.386072431590594 3.3767777919557247 5 0.7413652860042275 2.2576614472386924 6 0.431989739591558 1.5786332580017486 7 0.31581162334140106 1.3464267023222287 8 0.2213066012358174 1.0783032679017188 9 0.16705372335685978 0.9157042612175361 >10 0.986765104160775 11.424963495269 >50 0.07997260895802363 3.3215187646393285 >100 0.033911441885638376 3.518800602978326 >500 0.0010320862185788878 0.44127533877027036 >1k 4.423226651052377E-4 0.5142824308378791 >5k 2.9488177673682507E-4 1.1041761427593089 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7115 0.6375733679824365 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5181 0.4642681123706259 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3541 0.31730812312379586 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1130 0.10125901698104754 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.3441462431112506E-4 0.0 20-21 0.0 0.0 0.0 1.7921949908150008E-4 0.0 22-23 0.0 0.0 0.0 4.928536224741252E-4 0.0 24-25 0.0 0.0 0.0 7.616828710963753E-4 0.0 26-27 0.0 0.0 0.0 0.0011649267440297505 0.0 28-29 0.0 0.0 0.0 0.0020162193646668758 0.0 30-31 0.0 0.0 0.0 0.002957121734844751 0.0 32-33 0.0 0.0 0.0 0.004838926475200502 0.0 34-35 0.0 0.0 0.0 0.007796048210045254 0.0 36-37 0.0 0.0 0.0 0.010125901698104754 0.0 38-39 0.0 0.0 0.0 0.012186925937542004 0.0 40-41 0.0 0.0 0.0 0.014606389175142256 0.0 42-43 0.0 0.0 0.0 0.018997266902639007 0.0 44-45 0.0 0.0 0.0 0.02634526636498051 0.0 46-47 0.0 0.0 0.0 0.04390877727496752 0.0 48-49 0.0 0.0 0.0 0.08369550607106054 0.0 50-51 0.0 0.0 0.0 0.10766611407321117 0.0 52-53 0.0 0.0 0.0 0.1318607464492137 4.480487477037502E-5 54-55 0.0 0.0 0.0 0.16326896366324656 1.7921949908150008E-4 56-57 0.0 0.0 0.0 0.18965903490299746 1.7921949908150008E-4 58-59 0.0 0.0 0.0 0.21784130113356334 1.7921949908150008E-4 60-61 0.0 0.0 0.0 0.2660961512612572 1.7921949908150008E-4 62-63 0.0 0.0 0.0 0.30288095344773514 1.7921949908150008E-4 64-65 0.0 0.0 0.0 0.3381423898920203 1.7921949908150008E-4 66-67 0.0 0.0 0.0 0.40835162865719793 1.7921949908150008E-4 68-69 0.0 0.0 0.0 0.49128545185716205 1.7921949908150008E-4 70-71 0.0 0.0 0.0 0.558537568887495 1.7921949908150008E-4 72-73 0.0 0.0 0.0 0.6425019042071778 1.7921949908150008E-4 74-75 0.0 0.0 0.0 0.7178637035709485 1.7921949908150008E-4 76-77 0.0 0.0 0.0 0.7738697970339172 1.7921949908150008E-4 78-79 0.0 0.0 0.0 0.8462296697880729 1.7921949908150008E-4 80-81 0.0 0.0 0.0 0.9704735875263228 1.7921949908150008E-4 82-83 0.0 0.0 0.0 1.087100676553609 1.7921949908150008E-4 84-85 0.0 0.0 0.0 1.231910031811461 1.7921949908150008E-4 86-87 0.0 0.0 0.0 1.5068327434024822 1.7921949908150008E-4 88-89 0.0 0.0 0.0 1.8317576952372419 1.7921949908150008E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4825 0.0 33.288418 1 GTATCAA 7360 0.0 32.734367 1 TATCAAC 9020 0.0 26.604717 2 ATCAACG 9105 0.0 26.408539 3 TCAACGC 9130 0.0 26.284178 4 CAACGCA 9250 0.0 25.998064 5 GTACATG 5765 0.0 25.964802 1 AACGCAG 9410 0.0 25.70638 6 TACATGG 5895 0.0 24.747332 2 CGTCCGA 50 0.0016490655 23.76252 10-11 GAGTACT 5285 0.0 23.559134 12-13 ACATGGG 5895 0.0 23.538177 3 ACGCAGA 10310 0.0 23.417324 7 CGCAGAG 10405 0.0 23.158882 8 GTATAGG 350 0.0 23.081001 1 GCAGAGT 10680 0.0 21.761522 9 GTACTTT 5670 0.0 21.62224 14-15 AGTACTT 5545 0.0 20.826088 12-13 CATGGGA 3945 0.0 20.236528 4 TAGGACA 520 0.0 20.104494 4 >>END_MODULE