FastQCFastQC Report
Thu 26 May 2016
SRR936970_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936970_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1115950
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76590.6863210717326045No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59410.532371522021596No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41350.3705363143510014No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15350.1375509655450513No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA11950.1070836507011963No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA85650.030.6728461
GGTATCA57800.030.4931561
GTACACG1354.2196007E-728.1522121
GTACATG63200.025.181641
TATCAAC104650.025.006442
ATCAACG104550.024.9849323
TCAACGC104750.024.8907744
GTGTACG1351.3411811E-524.6331861
CAACGCA106100.024.5740665
AACGCAG108300.024.1614916
GTTGCGA500.001653820723.75076978-79
TGTACGT1401.7811362E-523.7470422
TACATGG66200.023.0296082
TTACGCC1050.002197771322.6162323
ACATGGG65550.022.605883
GAGTACT60400.022.52722712-13
ACGCAGA121700.021.3450587
AGTACTT62650.021.33916312-13
CGCAGAG122500.021.0505928
GTACTTT64650.020.90080514-15
Produced by FastQC (version 0.11.5)