##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936970_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1115950 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.20885523544962 33.0 31.0 34.0 31.0 34.0 2 32.28354137730185 33.0 31.0 34.0 31.0 34.0 3 32.41097271383126 34.0 31.0 34.0 31.0 34.0 4 36.08860163985842 37.0 35.0 37.0 35.0 37.0 5 36.00810072135848 37.0 35.0 37.0 35.0 37.0 6 36.04740086921457 37.0 35.0 37.0 35.0 37.0 7 36.00404588019177 37.0 35.0 37.0 35.0 37.0 8 35.98409426945652 37.0 35.0 37.0 35.0 37.0 9 37.676343922218734 39.0 37.0 39.0 35.0 39.0 10-11 37.57802455307137 39.0 37.0 39.0 35.0 39.0 12-13 37.500778260674764 39.0 37.0 39.0 35.0 39.0 14-15 38.69993503293158 40.0 38.0 41.0 34.5 41.0 16-17 38.62526188449303 40.0 38.0 41.0 34.0 41.0 18-19 38.67867915229177 40.0 38.0 41.0 34.5 41.0 20-21 38.78256194273937 40.0 38.0 41.0 35.0 41.0 22-23 38.7108687665218 40.0 38.0 41.0 34.5 41.0 24-25 38.62508938572517 40.0 38.0 41.0 34.0 41.0 26-27 38.448324745732336 40.0 38.0 41.0 34.0 41.0 28-29 38.288213629642904 40.0 38.0 41.0 34.0 41.0 30-31 38.223192347327384 40.0 38.0 41.0 34.0 41.0 32-33 38.12068551458398 40.0 37.0 41.0 34.0 41.0 34-35 37.99274340248219 40.0 37.0 41.0 33.0 41.0 36-37 37.816063443702674 40.0 37.0 41.0 33.0 41.0 38-39 37.66405887360545 40.0 37.0 41.0 33.0 41.0 40-41 37.43552578520543 40.0 37.0 41.0 33.0 41.0 42-43 37.18844123840674 40.0 36.0 41.0 32.0 41.0 44-45 36.90632913661006 40.0 36.0 41.0 31.0 41.0 46-47 36.4917169228012 39.0 36.0 41.0 30.5 41.0 48-49 36.08294412832116 39.0 35.0 41.0 29.5 41.0 50-51 35.82954612661858 39.0 35.0 40.5 29.0 41.0 52-53 35.594182535059815 39.0 35.0 40.0 28.0 41.0 54-55 35.319514763206236 38.5 35.0 40.0 27.5 41.0 56-57 35.07349074779336 38.0 34.5 40.0 27.0 41.0 58-59 34.74195573278372 38.0 34.0 40.0 26.0 41.0 60-61 34.41656794659259 38.0 34.0 40.0 26.0 41.0 62-63 34.07468479770599 37.0 34.0 40.0 25.5 41.0 64-65 33.66513508669743 36.5 33.0 39.5 24.5 41.0 66-67 33.240354854608185 36.0 33.0 39.0 23.0 40.0 68-69 32.809803754648506 35.5 32.0 39.0 22.0 40.0 70-71 32.40855414669116 35.0 32.0 38.0 22.0 40.0 72-73 32.02420359335096 35.0 31.5 37.5 20.5 39.5 74-75 31.545304449124068 35.0 31.0 37.0 18.5 39.0 76-77 30.084690174290962 33.5 29.5 35.5 17.5 38.0 78-79 30.786711322191856 34.0 31.0 36.0 17.5 38.0 80-81 30.484894036471168 34.0 31.0 36.0 11.0 37.0 82-83 30.29959182759084 34.0 30.0 35.0 12.0 37.0 84-85 29.964959003539583 34.0 30.0 35.0 7.5 36.5 86-87 29.590359783144407 34.0 30.0 35.0 4.5 36.0 88-89 29.21849007572024 34.0 29.0 35.0 2.0 36.0 90-91 28.77172140328868 34.0 29.0 35.0 2.0 35.0 92-93 28.3547609659931 34.0 29.0 35.0 2.0 35.0 94-95 27.8717366369461 33.0 28.0 35.0 2.0 35.0 96-97 27.239850351718268 33.0 27.0 35.0 2.0 35.0 98-99 26.46883731349971 33.0 25.5 34.5 2.0 35.0 100-101 23.29644518123572 29.0 11.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.25128297349309037 1101 2 0.17265392508254251 1101 3 0.14459723291832915 1101 4 0.148393571770292 1101 5 0.13417248041537277 1101 6 0.16265151614479123 1101 7 0.04348113868230996 1101 8 0.07439093052553858 1101 9 0.13307296685912462 1101 10-11 0.1528018370493811 1101 12-13 0.1637387546454434 1101 14-15 0.20388565009236714 1101 16-17 0.2735425474776676 1101 18-19 0.09751123794465144 1101 20-21 0.06524545297825313 1101 22-23 0.2749851548814206 1101 24-25 0.2561041690603503 1101 26-27 0.1825353061819186 1101 28-29 0.24055776619812264 1101 30-31 0.15447686937107363 1101 32-33 0.23704661384102366 1101 34-35 0.25970526080727296 1101 36-37 0.28589396257593336 1101 38-39 0.28610915240612655 1101 40-41 0.5347020521636239 1101 42-43 0.46626227942236653 1101 44-45 0.3996756165092208 1101 46-47 0.47036378590831873 1101 48-49 0.28989360817831056 1101 50-51 0.14471005081257005 1101 52-53 0.1114673293176267 1101 54-55 0.3404639984386293 1101 56-57 0.21381341915498098 1101 58-59 0.25219698839564586 1101 60-61 0.203070089248925 1101 62-63 0.504065708883978 1101 64-65 0.502124206367256 1101 66-67 0.4659995953899596 1101 68-69 0.41173885298136526 1101 70-71 0.2755214285127181 1101 72-73 0.40422884735904674 1101 74-75 0.3508977072112458 1101 76-77 0.2290613101067187 1101 78-79 0.4408667247340716 1101 80-81 0.6359072308473941 1101 82-83 0.4392123064423714 1101 84-85 0.4092616649372012 1101 86-87 0.26797391691728834 1101 88-89 0.0734371295558276 1101 90-91 0.014094669015921824 1101 92-93 0.24420967436420327 1101 94-95 0.22775659314459062 1101 96-97 0.307406482773807 1101 98-99 0.6278256508893172 1101 100-101 1.0792310513895451 1102 1 0.38129627031900526 1102 2 0.2648277644350969 1102 3 0.2190917416722371 1102 4 0.18384202185271903 1102 5 0.16379272387440835 1102 6 0.24998079115503202 1102 7 0.17790216179134433 1102 8 0.13118694026712774 1102 9 0.3559360418358679 1102 10-11 0.3701060720581708 1102 12-13 0.2984119937239882 1102 14-15 0.31558604980423155 1102 16-17 0.30474922938490323 1102 18-19 0.31114673946097327 1102 20-21 0.24606563913381763 1102 22-23 0.4280560356397629 1102 24-25 0.35236340268699706 1102 26-27 0.4282027402577029 1102 28-29 0.29312512299494387 1102 30-31 0.25917121388194886 1102 32-33 0.39185072274182886 1102 34-35 0.2787788412853516 1102 36-37 0.3356904747317273 1102 38-39 0.4539977213730708 1102 40-41 0.5714736272220904 1102 42-43 0.5099813955769008 1102 44-45 0.4986295255540938 1102 46-47 0.49540580508883636 1102 48-49 0.48157875386834803 1102 50-51 0.5180678726350934 1102 52-53 0.43722039575938254 1102 54-55 0.443163314630695 1102 56-57 0.35728849113741745 1102 58-59 0.45505429267100794 1102 60-61 0.4301345778457417 1102 62-63 0.20508940306101664 1102 64-65 0.25216809194595413 1102 66-67 0.16707625492059464 1102 68-69 0.32017083638757526 1102 70-71 0.2466780102397692 1102 72-73 0.2751956617906366 1102 74-75 0.3925315017298878 1102 76-77 0.5586601656515349 1102 78-79 0.5283619578522476 1102 80-81 0.49491446843685694 1102 82-83 0.43598394203266366 1102 84-85 0.4549813581618736 1102 86-87 0.3880302316989592 1102 88-89 0.3562685867386328 1102 90-91 0.35340989257006683 1102 92-93 0.5493188637017248 1102 94-95 0.8110488993468792 1102 96-97 0.6616458296756846 1102 98-99 0.7406156235894592 1102 100-101 0.8819527643596992 1103 1 0.17751242343018703 1103 2 0.16701753947700837 1103 3 0.20868357694396877 1103 4 0.11947955330455784 1103 5 0.09230117196364063 1103 6 0.16068624424072198 1103 7 0.17829950206925815 1103 8 0.1527103920481423 1103 9 0.18658664557803917 1103 10-11 0.16116934644927738 1103 12-13 0.17606523855011602 1103 14-15 0.30550584441618156 1103 16-17 0.2623728375418466 1103 18-19 0.22046867008413074 1103 20-21 0.18765559420774025 1103 22-23 0.25510676718622705 1103 24-25 0.32020828227138054 1103 26-27 0.20658056785093493 1103 28-29 0.14999711286071005 1103 30-31 0.2876373677611923 1103 32-33 0.2837529176372371 1103 34-35 0.3223736238388355 1103 36-37 0.4434142372164587 1103 38-39 0.47745129451696044 1103 40-41 0.5324364810264655 1103 42-43 0.5206519789809789 1103 44-45 0.6478151827989436 1103 46-47 0.4785767033953263 1103 48-49 0.5763613073531104 1103 50-51 0.6168266450994153 1103 52-53 0.574845038229931 1103 54-55 0.5025047538422385 1103 56-57 0.400562226842645 1103 58-59 0.5522403452079985 1103 60-61 0.4209291867164566 1103 62-63 0.4215722657133867 1103 64-65 0.37199690792312623 1103 66-67 0.3962519888992304 1103 68-69 0.47829511377614864 1103 70-71 0.6575980065940783 1103 72-73 0.7082791554767205 1103 74-75 0.532985657571249 1103 76-77 0.48851629365245586 1103 78-79 0.4662038061809106 1103 80-81 0.8138140918852592 1103 82-83 0.5919970931766088 1103 84-85 0.45461217988819413 1103 86-87 0.6090960765130013 1103 88-89 0.6486718604948187 1103 90-91 0.3864994794897889 1103 92-93 0.5219989033947883 1103 94-95 0.6329846428698751 1103 96-97 0.681689496143342 1103 98-99 0.7837697987858014 1103 100-101 0.8663650950164445 1104 1 0.15385174896742626 1104 2 0.08834172167285459 1104 3 0.12705289606705605 1104 4 0.12690202067097545 1104 5 0.019448000782290364 1104 6 0.15494749130444063 1104 7 0.14730343976279414 1104 8 0.12856600145612873 1104 9 0.20026662620815472 1104 10-11 0.10268801332550481 1104 12-13 0.2165208449846503 1104 14-15 0.19115423641844842 1104 16-17 0.2387551718677443 1104 18-19 0.18670331966338694 1104 20-21 0.1434391081425801 1104 22-23 0.17140608924028555 1104 24-25 0.14813064981493085 1104 26-27 0.2841376261772268 1104 28-29 0.248716353612231 1104 30-31 0.4030870273015026 1104 32-33 0.48619555134678905 1104 34-35 0.43068355897521116 1104 36-37 0.4469215773830584 1104 38-39 0.4597128240581583 1104 40-41 0.5469883188291718 1104 42-43 0.4405128004169825 1104 44-45 0.3732006158815224 1104 46-47 0.36414783886982605 1104 48-49 0.4654384555624631 1104 50-51 0.4872122268543464 1104 52-53 0.47042686749460216 1104 54-55 0.4389005961136121 1104 56-57 0.4325281107847516 1104 58-59 0.45924631758892076 1104 60-61 0.5417260068271545 1104 62-63 0.5711751512314223 1104 64-65 0.5424850736035935 1104 66-67 0.5320984337184242 1104 68-69 0.4819768784745335 1104 70-71 0.30663673361888044 1104 72-73 0.29087328631689147 1104 74-75 0.4049519450526091 1104 76-77 0.3721857504036663 1104 78-79 0.5678659631194485 1104 80-81 0.5185715010783056 1104 82-83 0.48416901576503335 1104 84-85 0.37869821658053127 1104 86-87 0.4604526586629767 1104 88-89 0.2939648406109576 1104 90-91 0.2663528354496414 1104 92-93 0.4830109055016898 1104 94-95 0.5879082465178698 1104 96-97 0.8655857844317865 1104 98-99 0.9667159114284765 1104 100-101 1.157373444031176 1105 1 0.10875461823169275 1105 2 0.12644432299025965 1105 3 0.114991938157047 1105 4 0.032178899090091306 1105 5 0.1457419608135453 1105 6 0.17930386062452186 1105 7 0.08680103940553607 1105 8 0.13450995590516612 1105 9 0.14523704040933438 1105 10-11 0.09863755000073127 1105 12-13 0.22180525940913043 1105 14-15 0.3326751139444113 1105 16-17 0.3441342050261724 1105 18-19 0.30194586739141727 1105 20-21 0.2695776031744046 1105 22-23 0.024474359866609063 1105 24-25 0.13198685166823054 1105 26-27 0.24032719857869722 1105 28-29 0.30016466219769455 1105 30-31 0.3078762982627552 1105 32-33 0.29510617990545285 1105 34-35 0.3358035768383374 1105 36-37 0.3081901382851484 1105 38-39 0.46587643910784493 1105 40-41 0.6392167435249405 1105 42-43 0.6236952275689376 1105 44-45 0.5389064883834749 1105 46-47 0.3797155512735344 1105 48-49 0.4866916653425193 1105 50-51 0.4622158553013733 1105 52-53 0.482902305699362 1105 54-55 0.4131442044646221 1105 56-57 0.38639408153388644 1105 58-59 0.5232113728529626 1105 60-61 0.5998721495088617 1105 62-63 0.6255647347973579 1105 64-65 0.5346384173570868 1105 66-67 0.41857465343485245 1105 68-69 0.44323734514864555 1105 70-71 0.33520545082330244 1105 72-73 0.2590085436575187 1105 74-75 0.24819214824564284 1105 76-77 0.18828684451999678 1105 78-79 0.5499849761818574 1105 80-81 0.569001890717697 1105 82-83 0.48211615208299463 1105 84-85 0.2506963744458872 1105 86-87 0.26331271225234687 1105 88-89 0.14737697637069758 1105 90-91 0.3278100755970108 1105 92-93 0.311720015694835 1105 94-95 0.2926966801452018 1105 96-97 0.7589962455236687 1105 98-99 0.9278072366489702 1105 100-101 0.9538228690618773 1106 1 0.1676225427599931 1106 2 0.0864443229902605 1106 3 0.05989759853440546 1106 4 0.0314241821089567 1106 5 0.010647621190905454 1106 6 0.08119065307735696 1106 7 -0.004519715311445793 1106 8 -0.0783202327740824 1106 9 0.09165213474896206 1106 10-11 0.04618471981205374 1106 12-13 -0.036307948138045276 1106 14-15 0.13607134035950708 1106 16-17 0.07017194087521972 1106 18-19 0.10571945229708746 1106 20-21 0.09599269751402772 1106 22-23 0.19617247307414942 1106 24-25 0.10972270072483781 1106 26-27 0.14447814197492903 1106 28-29 0.23374956785807655 1106 30-31 -0.0313689847561065 1106 32-33 -0.01772400877378999 1106 34-35 -0.008347366557885039 1106 36-37 -0.09067778624315537 1106 38-39 -0.086953749571407 1106 40-41 -0.04493419987128533 1106 42-43 -0.38347458375181986 1106 44-45 -0.22184822859765774 1106 46-47 -0.03764293929251039 1106 48-49 -0.07972342899709872 1106 50-51 -0.3751426352646732 1106 52-53 -0.48917316599875704 1106 54-55 -0.289874663459905 1106 56-57 -0.275870069409514 1106 58-59 -0.0884867403545897 1106 60-61 -0.07258068067982038 1106 62-63 -0.26235979350453675 1106 64-65 -0.25290875245423194 1106 66-67 -0.18218006354628358 1106 68-69 -0.12053623975701555 1106 70-71 -0.15347379445971399 1106 72-73 -0.11193485256889701 1106 74-75 -0.15105313477322468 1106 76-77 -0.22341126868754557 1106 78-79 -0.05114709928983885 1106 80-81 0.3157943435478856 1106 82-83 -0.06958196112454829 1106 84-85 -0.2911904180069449 1106 86-87 -0.18687596699293607 1106 88-89 -0.13790604249722804 1106 90-91 -0.19935973572374266 1106 92-93 -0.155072437135356 1106 94-95 0.24439479335274683 1106 96-97 0.4186188870331051 1106 98-99 0.48214685929048073 1106 100-101 0.6236341898165918 1107 1 -0.046305201497759185 1107 2 -0.094889561087804 1107 3 -0.045031665188730585 1107 4 -0.03975288724062409 1107 5 -0.03378272681091943 1107 6 0.0011198430400298776 1107 7 -0.012691193618501018 1107 8 -0.045964766386717315 1107 9 0.009818744865945916 1107 10-11 -0.0032333101936998787 1107 12-13 0.07607938276611037 1107 14-15 0.05087122824361501 1107 16-17 0.061136285071015095 1107 18-19 0.08026412900500901 1107 20-21 0.04852253361828929 1107 22-23 -0.046703299566623 1107 24-25 -0.002595000309284501 1107 26-27 0.0035201411783134517 1107 28-29 0.012053962506008986 1107 30-31 0.04631936256712521 1107 32-33 -0.019092707786874996 1107 34-35 -0.04769266875447187 1107 36-37 -0.1049070632431679 1107 38-39 -0.0775363096492967 1107 40-41 -0.1526672460305747 1107 42-43 -0.04385518770242669 1107 44-45 0.14938283340538305 1107 46-47 0.161900335966763 1107 48-49 0.23876300645511606 1107 50-51 0.15382752125501753 1107 52-53 0.2808889403048127 1107 54-55 0.20721533904794853 1107 56-57 0.2989076903379342 1107 58-59 0.16995838924253093 1107 60-61 0.09478208799263399 1107 62-63 0.4077179500156163 1107 64-65 0.12492888603102159 1107 66-67 0.06919084828046351 1107 68-69 0.2892747859558824 1107 70-71 0.054698273930981145 1107 72-73 0.10375901203640936 1107 74-75 0.10552556025679749 1107 76-77 0.08117073081088222 1107 78-79 0.06900868279226913 1107 80-81 0.2511860853272836 1107 82-83 0.12398996269565288 1107 84-85 0.169319414261782 1107 86-87 0.2030507151142551 1107 88-89 -0.001164779423188378 1107 90-91 -0.056586047553444985 1107 92-93 0.08702413005304166 1107 94-95 -0.009457715240344555 1107 96-97 -0.17422752894636417 1107 98-99 0.0476691570761929 1107 100-101 -0.1018258541446393 1108 1 0.020943053231995634 1108 2 -0.0558698981735688 1108 3 0.03257940838968665 1108 4 -0.053846624510079266 1108 5 -0.033579038148410234 1108 6 -0.0022928909172748035 1108 7 0.0061006669319425555 1108 8 0.04309272442198875 1108 9 0.01918526047406033 1108 10-11 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-1.119048136493646 2316 52-53 -1.1937566393237162 2316 54-55 -1.123016075636528 2316 56-57 -1.1210614895435427 2316 58-59 -1.092393656235835 2316 60-61 -1.0631739504716862 2316 62-63 -1.1213387682353115 2316 64-65 -1.0877031857975865 2316 66-67 -1.2090559808598584 2316 68-69 -1.4899942511096 2316 70-71 -0.995655541908917 2316 72-73 -1.098689817114174 2316 74-75 -1.114067051414839 2316 76-77 -1.244160680722704 2316 78-79 -1.162038153382607 2316 80-81 -1.4049497635412926 2316 82-83 -1.321298464811484 2316 84-85 -1.4698107783896042 2316 86-87 -1.5006002159010023 2316 88-89 -1.708091538774923 2316 90-91 -1.8329983981700302 2316 92-93 -1.6160457178907812 2316 94-95 -1.5121912970788962 2316 96-97 -1.6783544644549337 2316 98-99 -1.853614135210055 2316 100-101 -2.52501004282297 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 5.0 4 8.0 5 30.0 6 66.0 7 117.0 8 290.0 9 606.0 10 996.0 11 1766.0 12 2633.0 13 3730.0 14 4679.0 15 6760.0 16 9180.0 17 13306.0 18 11798.0 19 6784.0 20 5542.0 21 5684.0 22 5904.0 23 6312.0 24 7458.0 25 8727.0 26 10502.0 27 13165.0 28 16474.0 29 20434.0 30 25881.0 31 32496.0 32 41722.0 33 55041.0 34 75656.0 35 108797.0 36 166038.0 37 249583.0 38 183888.0 39 13890.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.43792603667243 19.866518593013467 15.175039729810399 21.520515640503703 2 15.561241178447407 21.503125350061612 39.95384787722639 22.98178559426459 3 19.219590885667557 26.347769587524926 30.3340577599534 24.09858176685412 4 12.89664671055756 17.78862654117706 37.59589371915742 31.718833029107962 5 13.251862180705013 38.31978965781758 33.85225863342104 14.576089528056377 6 30.46928625834491 37.47210896545544 17.49943993906537 14.559164837134281 7 27.533850082889018 34.02482190062279 20.94475558940813 17.496572427080068 8 26.402041679510802 34.696991963754456 19.752156022165988 19.14881033456876 9 27.038845826425916 16.764191944083517 20.60567229714593 35.59128993234464 10-11 24.61046641874636 27.47273623370223 27.15023074510507 20.766566602446346 12-13 26.154008141063983 24.951263388286634 27.895891159754377 20.998837310895002 14-15 22.985079976701464 25.814552623325415 27.055692459339575 24.14467494063354 16-17 21.446086942106252 29.34886390468386 27.42919818504332 21.775850968166573 18-19 21.367627449152966 27.216021923189977 30.761692845928295 20.654657781728762 20-21 22.353656482092163 26.31952011584894 30.90078642502796 20.42603697703094 22-23 22.004256463103186 26.323670415341187 30.76584972445002 20.906223397105606 24-25 21.846472043666957 26.43133217014771 30.919454324353147 20.802741461832188 26-27 21.694295696108608 26.639650752698195 30.82767004320784 20.838383507985363 28-29 21.34433523693449 26.645547074361424 31.028459920196617 20.98165776850746 30-31 21.438133700603117 26.547357768486478 31.00398478154962 21.010523749360782 32-33 21.353849297097348 26.7727144014395 30.81297090176568 21.060465399697474 34-35 21.811051035281245 26.644349942132145 30.547596142859895 20.997002879726715 36-37 21.871499117217397 26.463178555104456 30.552657758180302 21.112664569497845 38-39 21.670630681655243 26.726721344383584 30.66207414470477 20.9405738292564 40-41 21.72306545198564 26.614634513556272 30.665722093993942 20.99657794046414 42-43 21.635140372848927 26.846511794635113 30.306734446518067 21.21161338599789 44-45 21.73223809402193 27.03817162602533 29.93896070553413 21.290629574418606 46-47 21.95496133270008 27.25036516627387 29.281764985258935 21.51290851576712 48-49 22.199331499287588 27.349027268735494 28.993619671484772 21.458021560492146 50-51 22.15967287948319 27.553119894902768 28.748547161577136 21.538660064036904 52-53 22.33953465239621 27.68396616778017 28.610781163678173 21.365718016145443 54-55 21.948205509398218 27.587271418131703 28.74787340956575 21.716649662904334 56-57 22.10491745105489 28.02223946721341 28.361190167248534 21.511652914483165 58-59 22.18236545765657 27.621633949121836 28.551925483482314 21.644075109739276 60-61 22.275594784712577 27.491419866481476 28.782875576862764 21.450109771943186 62-63 22.225010081096823 27.52695013217438 28.909628567588154 21.338411219140642 64-65 22.0646821789068 27.528035262124718 28.74408029956854 21.663202259399938 66-67 21.97944786581746 27.825358385400683 28.457738637967456 21.737455110814402 68-69 22.01399716830654 27.769324515654965 28.608168897969428 21.608509418069072 70-71 22.094612850281678 27.784521180332924 28.39770034290538 21.72316562648002 72-73 22.17354891585139 27.685367701785978 28.275825294097352 21.86525808826528 74-75 22.16495376579058 28.0063213861167 28.092216602913627 21.736508245179092 76-77 22.12377722894719 27.952393924947216 28.16043078820118 21.763398057904414 78-79 22.159129863163965 27.85630715224482 28.14022276800698 21.84434021658424 80-81 22.25576616852562 28.26903379993172 27.841774131255104 21.63342590028756 82-83 22.210602298862938 28.267196105025587 27.777994344472777 21.7442072516387 84-85 22.17123875050518 27.996691575435573 27.687086838975965 22.14498283508328 86-87 22.178905864958107 27.9302836148573 27.910972713831267 21.97983780635333 88-89 22.314536522769266 27.745861314378807 27.900574765174284 22.03902739767764 90-91 22.099510637211107 27.89314130406536 28.054798595983122 21.952549462740414 92-93 22.06367235017776 27.82778759753483 28.012249680204487 22.096290372082915 94-95 21.906072347716375 27.70873807287821 27.9272078168797 22.45798176252572 96-97 22.050653861990696 27.66790343443884 27.92029008559102 22.361152617979446 98-99 21.91580058533298 27.770205726800402 27.62629195319487 22.68770173467175 100-101 21.855137243164723 28.074742436638562 27.571802759828266 22.498317560368445 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 821.0 1 562.5 2 360.5 3 438.5 4 400.0 5 384.0 6 633.0 7 1345.0 8 2204.5 9 2557.0 10 2258.0 11 1850.5 12 1818.5 13 1981.0 14 2115.5 15 2207.5 16 2260.0 17 2321.0 18 2384.0 19 2509.0 20 2775.5 21 3146.5 22 3624.5 23 4315.0 24 5003.0 25 5957.0 26 7445.5 27 8971.0 28 10209.5 29 11563.0 30 13803.0 31 16076.5 32 18292.5 33 21022.5 34 24108.5 35 26413.0 36 29253.0 37 32201.5 38 33266.5 39 33697.5 40 34014.5 41 35034.5 42 36077.5 43 37374.5 44 38921.5 45 39836.5 46 39490.5 47 39880.5 48 44813.5 49 44874.5 50 40214.0 51 37883.5 52 35594.5 53 34275.0 54 33127.5 55 31020.5 56 28017.5 57 24511.0 58 21977.5 59 20178.0 60 17655.5 61 14428.5 62 11520.5 63 9057.0 64 6605.5 65 4923.0 66 3621.0 67 2777.5 68 1986.5 69 1173.5 70 834.0 71 604.0 72 443.0 73 345.0 74 264.5 75 179.5 76 118.5 77 71.0 78 34.5 79 14.5 80 7.5 81 6.5 82 4.0 83 1.5 84 1.5 85 2.5 86 1.5 87 1.0 88 1.0 89 1.0 90 1.0 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02589721761727676 2 0.006720731215556253 3 0.011201218692593754 4 8.960974954075004E-5 5 0.0048389264752005025 6 0.0 7 0.0 8 5.376584972445002E-4 9 0.0 10-11 0.0 12-13 2.2402437385187507E-4 14-15 0.0 16-17 4.480487477037502E-5 18-19 0.0048389264752005025 20-21 0.0012545364935705005 22-23 0.0 24-25 0.004390877727496752 26-27 0.005779828845378377 28-29 0.007392804337111878 30-31 0.029616022223217884 32-33 5.376584972445002E-4 34-35 0.0040772436041041265 36-37 0.014337559926520005 38-39 0.006989560464178503 40-41 0.009095389578386128 42-43 0.007392804337111878 44-45 0.0026434876114521263 46-47 0.0017921949908150006 48-49 0.0017921949908150006 50-51 0.002777902235763251 52-53 0.0028227071105336263 54-55 0.001836999865585376 56-57 5.376584972445002E-4 58-59 8.512926206371254E-4 60-61 0.0 62-63 0.0 64-65 0.007034365338948878 66-67 6.720731215556253E-4 68-69 8.960974954075003E-4 70-71 0.003987633854563377 72-73 0.010797974819660378 74-75 0.004973341099511627 76-77 0.0037636094807115016 78-79 0.011246023567364129 80-81 8.960974954075004E-5 82-83 0.004525292351807877 84-85 9.857072449482504E-4 86-87 0.0 88-89 0.0014337559926520006 90-91 8.064877458667502E-4 92-93 2.2402437385187507E-4 94-95 5.376584972445002E-4 96-97 3.1363412339262514E-4 98-99 3.5843899816300016E-4 100-101 9.857072449482504E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1115950.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.12899635236442 #Duplication Level Percentage of deduplicated Percentage of total 1 79.94222210440053 47.26903359211005 2 11.916544781020736 14.092266657795246 3 3.51914577830693 6.242506736668473 4 1.4482206757096416 3.425273402058165 5 0.7985257283876714 2.360801244055188 6 0.47135969489671925 1.6722615408119832 7 0.3293435474813221 1.3631627392388497 8 0.22572174963330485 1.0677360408573597 9 0.16107714530134246 0.8571896943275117 >10 1.065088899095666 11.930521416779833 >50 0.08291164324738032 3.3613799818588688 >100 0.037869062760959395 3.955250460428112 >500 9.088568113507266E-4 0.3758202723083628 >1k 7.573806761256055E-4 0.8086942894833671 >5k 3.029522704502422E-4 1.2181019312187165 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7659 0.6863210717326045 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5941 0.532371522021596 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4135 0.3705363143510014 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1535 0.1375509655450513 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1195 0.1070836507011963 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.3441462431112506E-4 0.0 20-21 0.0 0.0 0.0 1.7921949908150008E-4 0.0 22-23 0.0 0.0 0.0 4.032438729333752E-4 0.0 24-25 0.0 0.0 0.0 6.720731215556253E-4 0.0 26-27 0.0 0.0 0.0 0.0010753169944890005 0.0 28-29 0.0 0.0 0.0 0.0020610242394372507 0.0 30-31 0.0 0.0 0.0 0.003046731484385501 0.0 32-33 0.0 0.0 0.0 0.005018145974282002 0.0 34-35 0.0 0.0 0.0 0.007885657959586003 0.0 36-37 0.0 0.0 0.0 0.010394730946727004 0.0 38-39 0.0 0.0 0.0 0.012455755186164255 0.0 40-41 0.0 0.0 0.0 0.014830413548994132 0.0 42-43 0.0 0.0 0.0 0.01904207177740938 0.0 44-45 0.0 0.0 0.0 0.026748510237913886 0.0 46-47 0.0 0.0 0.0 0.04435682602267127 0.0 48-49 0.0 0.0 0.0 0.08432277431784578 0.0 50-51 0.0 0.0 0.0 0.10878623594247054 0.0 52-53 0.0 0.0 0.0 0.13333930731663607 0.0 54-55 0.0 0.0 0.0 0.16515076840360232 0.0 56-57 0.0 0.0 0.0 0.19145122989381247 0.0 58-59 0.0 0.0 0.0 0.21954388637483757 0.0 60-61 0.0 0.0 0.0 0.267977956001613 0.0 62-63 0.0 0.0 0.0 0.30458353868900934 0.0 64-65 0.0 0.0 0.0 0.33921770688650926 0.0 66-67 0.0 0.0 0.0 0.4097853846498499 0.0 68-69 0.0 0.0 0.0 0.4929880370984363 0.0 70-71 0.0 0.0 0.0 0.5601953492539988 0.0 72-73 0.0 0.0 0.0 0.6439804650746002 0.0 74-75 0.0 0.0 0.0 0.7183117523186522 0.0 76-77 0.0 0.0 0.0 0.7741386262825396 0.0 78-79 0.0 0.0 0.0 0.8458264259151396 0.0 80-81 0.0 0.0 0.0 0.9693982705318338 0.0 82-83 0.0 0.0 0.0 1.0862941888077424 0.0 84-85 0.0 0.0 0.0 1.2318204220619204 0.0 86-87 0.0 0.0 0.0 1.504906133787356 0.0 88-89 0.0 0.0 0.0 1.8294726466239526 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 8565 0.0 30.672846 1 GGTATCA 5780 0.0 30.493156 1 GTACACG 135 4.2196007E-7 28.152212 1 GTACATG 6320 0.0 25.18164 1 TATCAAC 10465 0.0 25.00644 2 ATCAACG 10455 0.0 24.984932 3 TCAACGC 10475 0.0 24.890774 4 GTGTACG 135 1.3411811E-5 24.633186 1 CAACGCA 10610 0.0 24.574066 5 AACGCAG 10830 0.0 24.161491 6 GTTGCGA 50 0.0016538207 23.750769 78-79 TGTACGT 140 1.7811362E-5 23.747042 2 TACATGG 6620 0.0 23.029608 2 TTACGCC 105 0.0021977713 22.616232 3 ACATGGG 6555 0.0 22.60588 3 GAGTACT 6040 0.0 22.527227 12-13 ACGCAGA 12170 0.0 21.345058 7 AGTACTT 6265 0.0 21.339163 12-13 CGCAGAG 12250 0.0 21.050592 8 GTACTTT 6465 0.0 20.900805 14-15 >>END_MODULE