FastQCFastQC Report
Thu 26 May 2016
SRR936969_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936969_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1177010
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75230.6391619442485621No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53370.4534370990900672No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36240.30789882838718446No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12370.10509681311118853No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA77250.035.000161
GGTATCA48350.032.923391
ATCAACG95050.028.2969073
TCAACGC95700.028.1047154
TATCAAC96400.027.9992222
CAACGCA97700.027.480755
AACGCAG100250.026.7806026
GTACATG64950.025.3867191
ACGCAGA109950.024.2862457
TACATGG65850.024.247012
CGCAGAG110500.024.1653658
ACATGGG66000.023.2559073
TCGCGGA1453.6379788E-1222.93523882-83
AGTACTT57250.022.90278612-13
GCAGAGT113850.022.8680929
GAGTACT54850.022.64903812-13
CATGGGA42300.022.1309764
GTATAGG3750.021.5413991
TCGTATG1353.790783E-421.1154259
CGCGGAA1351.0004442E-921.11497982-83
Produced by FastQC (version 0.11.5)