FastQCFastQC Report
Thu 26 May 2016
SRR936969_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936969_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1177010
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76450.6495271917825677No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60030.5100211553002948No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41530.3528432213829959No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14600.1240431262266251No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA12500.10620130670087764No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63050.031.8759461
GTATCAA91450.028.8348121
GTACATG61400.024.0657751
TATCAAC110850.023.6527732
ATCAACG110950.023.5000253
TCAACGC111250.023.4366574
GAGTACT63050.023.16238812-13
CAACGCA112900.023.1029685
TACATGG63150.022.9406192
AACGCAG115750.022.6162176
ACATGGG62600.022.3046953
GTACTTT65900.021.80126814-15
AGTACTT65750.021.0194112-13
ACGCAGA125600.020.653447
CGCAGAG126100.020.4585158
CATGGGG23700.020.038954
GTCCGCG1456.137037E-419.6595068
GGTCCGC1707.940596E-519.5631377
GCAGAGT131250.019.4023749
TGTACGG1250.00602354919.004195
Produced by FastQC (version 0.11.5)