##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936969_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1177010 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.20122938632637 33.0 31.0 34.0 31.0 34.0 2 32.327578355324086 33.0 31.0 34.0 31.0 34.0 3 32.42416801896331 34.0 31.0 34.0 31.0 34.0 4 36.07801717912337 37.0 35.0 37.0 35.0 37.0 5 35.99305358493131 37.0 35.0 37.0 35.0 37.0 6 36.04023415264101 37.0 35.0 37.0 35.0 37.0 7 35.98612841012396 37.0 35.0 37.0 35.0 37.0 8 35.94975743621549 37.0 35.0 37.0 35.0 37.0 9 37.63116286182785 39.0 37.0 39.0 35.0 39.0 10-11 37.54671413157068 39.0 37.0 39.0 35.0 39.0 12-13 37.44737640291926 39.0 37.0 39.0 35.0 39.0 14-15 38.62963823586885 40.0 38.0 41.0 34.0 41.0 16-17 38.53615942090552 40.0 38.0 41.0 34.0 41.0 18-19 38.71708566622204 40.0 38.0 41.0 34.5 41.0 20-21 38.74898046745567 40.0 38.0 41.0 34.0 41.0 22-23 38.66913874988318 40.0 38.0 41.0 34.0 41.0 24-25 38.55479562620539 40.0 38.0 41.0 34.0 41.0 26-27 38.369501958352096 40.0 38.0 41.0 34.0 41.0 28-29 38.28001546291026 40.0 38.0 41.0 34.0 41.0 30-31 38.25508449375961 40.0 38.0 41.0 34.0 41.0 32-33 38.07279632288596 40.0 37.0 41.0 33.5 41.0 34-35 37.915670640011555 40.0 37.0 41.0 33.0 41.0 36-37 37.74581821734735 40.0 37.0 41.0 33.0 41.0 38-39 37.58026354916271 40.0 37.0 41.0 33.0 41.0 40-41 37.37941903637183 40.0 36.5 41.0 32.5 41.0 42-43 37.11184399452851 40.0 36.0 41.0 31.5 41.0 44-45 36.80541966508356 39.0 36.0 41.0 31.0 41.0 46-47 36.39362919601363 39.0 35.5 41.0 30.5 41.0 48-49 35.99281272036771 39.0 35.0 40.5 29.5 41.0 50-51 35.68709569162539 39.0 35.0 40.0 28.0 41.0 52-53 35.43082981453004 39.0 35.0 40.0 27.5 41.0 54-55 35.14081953424355 38.0 35.0 40.0 27.0 41.0 56-57 34.85316607335537 38.0 34.0 40.0 26.0 41.0 58-59 34.50248171213499 38.0 34.0 40.0 26.0 41.0 60-61 34.1634922388085 37.5 34.0 40.0 25.0 41.0 62-63 33.79492952481287 37.0 33.0 39.5 24.0 41.0 64-65 33.40191077391016 36.0 33.0 39.0 23.0 40.0 66-67 33.00726544379402 36.0 32.0 39.0 22.0 40.0 68-69 32.70075955174552 35.5 32.0 39.0 22.0 40.0 70-71 32.22863442111792 35.0 31.5 38.0 20.5 40.0 72-73 31.72195096048462 35.0 31.0 37.0 18.5 39.5 74-75 31.203819848599416 34.5 31.0 37.0 18.0 39.0 76-77 29.79756331721906 33.5 28.5 35.0 16.0 37.5 78-79 30.548909100177568 34.0 30.0 36.0 16.0 38.0 80-81 30.320898717937823 34.0 30.0 35.5 10.5 37.0 82-83 29.986329342996235 34.0 30.0 35.0 7.5 37.0 84-85 29.622581796246422 34.0 30.0 35.0 7.0 36.0 86-87 29.24986958479537 34.0 29.0 35.0 2.0 36.0 88-89 28.84297117271731 34.0 29.0 35.0 2.0 35.5 90-91 28.376040985208284 33.5 29.0 35.0 2.0 35.0 92-93 27.878376989150475 33.0 27.5 35.0 2.0 35.0 94-95 27.269046567148962 33.0 26.5 35.0 2.0 35.0 96-97 26.57318926772075 32.5 25.0 34.0 2.0 35.0 98-99 25.714535985250762 32.0 24.0 34.0 2.0 35.0 100-101 22.17472918666791 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.16681488822098345 1101 2 0.1395158346814469 1101 3 0.09934173959756265 1101 4 0.11016088686053394 1101 5 0.09196471207321366 1101 6 0.12227625180981505 1101 7 0.1727492303874456 1101 8 0.16010218697744705 1101 9 0.229146292996532 1101 10-11 0.27807733199091444 1101 12-13 0.2767350070862946 1101 14-15 0.31691476010080777 1101 16-17 0.28968500941710573 1101 18-19 0.09858016790953172 1101 20-21 0.1663978705202993 1101 22-23 0.1744554579422939 1101 24-25 0.19660758228480546 1101 26-27 0.12116980094538121 1101 28-29 0.14277991595012907 1101 30-31 0.2224593766281231 1101 32-33 0.34881342303746266 1101 34-35 0.23930881058588227 1101 36-37 0.13342142740327745 1101 38-39 0.12448650014840723 1101 40-41 0.2546594395073143 1101 42-43 0.3369364829822743 1101 44-45 0.2782881901696257 1101 46-47 0.3817953714796971 1101 48-49 0.6236599815240211 1101 50-51 0.26363322855436877 1101 52-53 0.1957868470042996 1101 54-55 0.30240858876317134 1101 56-57 0.3359567118109865 1101 58-59 0.2253069975126749 1101 60-61 0.1824215114864316 1101 62-63 0.06035140394077132 1101 64-65 0.21080775558278475 1101 66-67 0.1535916802783177 1101 68-69 0.19704272786099608 1101 70-71 0.13689486445371557 1101 72-73 0.19329474613518016 1101 74-75 0.2768110612723582 1101 76-77 0.056824987579730646 1101 78-79 0.2153570683110324 1101 80-81 0.40032356054966556 1101 82-83 0.49657762010355455 1101 84-85 0.34780860489488674 1101 86-87 -0.07523651133898923 1101 88-89 -0.15260973603481176 1101 90-91 0.09900373291489473 1101 92-93 0.18672492550580344 1101 94-95 -0.05745695623747693 1101 96-97 0.0020641384361539394 1101 98-99 0.24846668490150492 1101 100-101 0.6640100563754565 1102 1 0.3356595609037569 1102 2 0.3280694239677615 1102 3 0.2508175350021773 1102 4 0.035128529902145544 1102 5 0.17248035574307607 1102 6 0.1596930233508047 1102 7 0.14446596832888758 1102 8 0.17344408918885534 1102 9 0.21213975400814178 1102 10-11 0.2054130968179564 1102 12-13 0.15997236302079187 1102 14-15 0.2352904805525995 1102 16-17 0.2880872914027819 1102 18-19 0.08942454038334802 1102 20-21 0.2785448019275236 1102 22-23 0.2605667181638083 1102 24-25 0.2679956808885393 1102 26-27 0.16269829196793495 1102 28-29 0.31230627171164826 1102 30-31 0.3357666355215656 1102 32-33 0.4058257872724411 1102 34-35 0.32732296812201866 1102 36-37 0.29893845282958154 1102 38-39 0.34567116245310103 1102 40-41 0.2617670355890951 1102 42-43 0.45506845762157155 1102 44-45 0.47458234921675313 1102 46-47 0.4530639332402302 1102 48-49 0.511194699397187 1102 50-51 0.5024844919171443 1102 52-53 0.45504734885028597 1102 54-55 0.5468239069747227 1102 56-57 0.4462698337256441 1102 58-59 0.3054329659847639 1102 60-61 0.34629534022418795 1102 62-63 0.43043200308626695 1102 64-65 0.37950431192764 1102 66-67 0.36984153365308003 1102 68-69 0.3271435463777195 1102 70-71 0.20985707192395608 1102 72-73 0.42341460108058016 1102 74-75 0.21551773724351975 1102 76-77 0.2085473121911008 1102 78-79 0.23761179660204945 1102 80-81 0.5037922662514482 1102 82-83 0.3388536015979575 1102 84-85 0.27772745901122775 1102 86-87 0.26485251751486416 1102 88-89 0.26698595606611164 1102 90-91 0.3080816242153972 1102 92-93 0.21214551626135503 1102 94-95 0.0488276995063579 1102 96-97 0.29359088983729364 1102 98-99 0.5148260847221735 1102 100-101 0.8534617873484684 1103 1 0.12583923480436 1103 2 0.15230311448052447 1103 3 0.07121139609371596 1103 4 0.061709184389776794 1103 5 -0.0179812068115055 1103 6 -0.06434220068442187 1103 7 -0.03791237684015414 1103 8 -0.008806261666144621 1103 9 0.07808603855907847 1103 10-11 0.08892547160940012 1103 12-13 0.13944583958728884 1103 14-15 0.15359170990033277 1103 16-17 0.09376255928734878 1103 18-19 0.1496247837231266 1103 20-21 0.014198898046743125 1103 22-23 0.12338994145214599 1103 24-25 0.07874178233230822 1103 26-27 0.0788326528697425 1103 28-29 -0.06859501907335641 1103 30-31 0.009151497859917868 1103 32-33 2.8096591030646323E-5 1103 34-35 0.0988749518833032 1103 36-37 -0.07921246601901544 1103 38-39 -0.0018748100928718259 1103 40-41 -0.019690955171220992 1103 42-43 -0.06387997121057509 1103 44-45 0.006468744475242261 1103 46-47 -0.01889100673796662 1103 48-49 0.050912840859517416 1103 50-51 0.16372947002259508 1103 52-53 0.04390777782699473 1103 54-55 0.2861326172136671 1103 56-57 0.25218753789916803 1103 58-59 0.22032427622491468 1103 60-61 0.20261795817471295 1103 62-63 0.43838120289593974 1103 64-65 0.12335907903821663 1103 66-67 -3.75243540439385E-4 1103 68-69 -0.11889706245359122 1103 70-71 -0.22351812307915253 1103 72-73 -0.17853738749931836 1103 74-75 0.1052288013731868 1103 76-77 -0.03504996047594133 1103 78-79 0.09540710207177128 1103 80-81 0.2763358294926874 1103 82-83 0.2623130018015445 1103 84-85 0.23688748503171908 1103 86-87 0.23092348358583337 1103 88-89 0.08434861063806665 1103 90-91 -0.26140950050828593 1103 92-93 -0.16133059849383002 1103 94-95 -0.4726806156066097 1103 96-97 -0.3604118688166267 1103 98-99 -0.48881648287387947 1103 100-101 -0.3698928080991486 1104 1 0.22575657125393178 1104 2 0.16963421173507953 1104 3 0.17357890396608155 1104 4 0.1317962784686273 1104 5 0.13540725537883702 1104 6 0.0735557663274733 1104 7 0.07544076006317368 1104 8 0.10879155934645723 1104 9 0.21729680979810695 1104 10-11 0.24412257065401377 1104 12-13 0.16851486365190027 1104 14-15 0.03457552509080131 1104 16-17 0.15545989769943702 1104 18-19 0.12923394569908453 1104 20-21 0.14132384192176062 1104 22-23 0.13371396472877706 1104 24-25 0.07146857929814843 1104 26-27 -0.016279493022501867 1104 28-29 -0.06441575216227591 1104 30-31 0.009352953095223882 1104 32-33 -0.02187625751820832 1104 34-35 0.12739239002688407 1104 36-37 3.341035923227764E-4 1104 38-39 -0.0015871230963284688 1104 40-41 0.19202957661112663 1104 42-43 0.06451614776546677 1104 44-45 -0.055589131904092426 1104 46-47 -0.006531616545188967 1104 48-49 -0.3720457708685956 1104 50-51 -0.23734114189583266 1104 52-53 -0.32574622466481173 1104 54-55 -0.2159785741821878 1104 56-57 -0.279175548365302 1104 58-59 -0.22897144816648307 1104 60-61 -0.24699147141705424 1104 62-63 -0.3030870563821537 1104 64-65 -0.3652665056279929 1104 66-67 -0.250907501652911 1104 68-69 -0.3787059252945326 1104 70-71 -0.5799117621486793 1104 72-73 -0.39262185894322243 1104 74-75 -0.44165525853707166 1104 76-77 -0.4640247243049913 1104 78-79 -0.3213517433741515 1104 80-81 0.15673683691753837 1104 82-83 0.02532805073063571 1104 84-85 0.17017309354547194 1104 86-87 0.027177159586344146 1104 88-89 -0.13969116896544165 1104 90-91 -0.20944966072095283 1104 92-93 -0.10357538964942492 1104 94-95 -0.1464777568750506 1104 96-97 -0.1159983488658689 1104 98-99 0.027412185915871135 1104 100-101 -0.08685340613127934 1105 1 0.23570433094852916 1105 2 0.19629083218917032 1105 3 0.1269815561662 1105 4 0.15465844443205867 1105 5 0.10934034760307298 1105 6 0.09596082961861185 1105 7 0.13980784867077034 1105 8 0.11232280306757048 1105 9 0.29089636776475203 1105 10-11 0.1635408949457542 1105 12-13 0.20232991287834068 1105 14-15 0.14515820542031577 1105 16-17 0.20853906185455173 1105 18-19 0.12912946508827616 1105 20-21 0.22213658051930452 1105 22-23 0.21316745076454424 1105 24-25 0.18600544889830672 1105 26-27 0.08877073804038815 1105 28-29 0.24642511583049043 1105 30-31 0.23523651749144392 1105 32-33 0.1271261535728101 1105 34-35 0.21756920336825658 1105 36-37 0.2435996249907575 1105 38-39 0.33925073103267067 1105 40-41 0.2552458715679293 1105 42-43 0.3911185186716395 1105 44-45 0.32091629305069347 1105 46-47 0.2606504208267211 1105 48-49 0.29567373887623205 1105 50-51 0.37244989688255004 1105 52-53 0.35160107290401044 1105 54-55 0.4931659908168058 1105 56-57 0.4079894104452251 1105 58-59 0.3035556766074734 1105 60-61 0.3067959507247977 1105 62-63 0.2732596384139043 1105 64-65 0.1397128996362298 1105 66-67 0.24602488733643924 1105 68-69 0.20265433188850324 1105 70-71 -0.23517447060759267 1105 72-73 -0.04564828048230396 1105 74-75 0.2528986896244696 1105 76-77 0.055435794079578216 1105 78-79 0.07742864641889824 1105 80-81 0.2548657298249104 1105 82-83 0.2359700037143604 1105 84-85 0.31009925388302406 1105 86-87 0.0707611459234947 1105 88-89 0.12568355476371096 1105 90-91 0.25553074916452445 1105 92-93 0.3135669651828046 1105 94-95 0.06306966124831703 1105 96-97 0.2112394449858499 1105 98-99 0.1705963329924245 1105 100-101 0.5220932484799299 1106 1 0.0832627725069699 1106 2 0.051122001020338814 1106 3 -0.011680781496139048 1106 4 0.01874935352297058 1106 5 -0.04397134070861597 1106 6 0.08886992052769926 1106 7 0.0015351213980423495 1106 8 0.1298942316389997 1106 9 0.11150675737514604 1106 10-11 0.03622920663406859 1106 12-13 0.16192082196925384 1106 14-15 0.22004132230343743 1106 16-17 0.07478581510130056 1106 18-19 -0.13793546997666084 1106 20-21 0.03142229480501868 1106 22-23 -0.03259878300169561 1106 24-25 0.07003142292428066 1106 26-27 0.013251257520906279 1106 28-29 0.13916537557075515 1106 30-31 0.12730794606287077 1106 32-33 0.14706121850787213 1106 34-35 0.21214063193968968 1106 36-37 0.17216456005569114 1106 38-39 0.20054943233137124 1106 40-41 0.3008108066328603 1106 42-43 0.19272890828202804 1106 44-45 0.18570200733640974 1106 46-47 0.33206600524229657 1106 48-49 0.28489451809701194 1106 50-51 0.24450184493449711 1106 52-53 0.3219712027741366 1106 54-55 0.24300365315446726 1106 56-57 0.16305434551016162 1106 58-59 0.2878478843996817 1106 60-61 0.33817257410142076 1106 62-63 0.358668729323 1106 64-65 0.21568043210375976 1106 66-67 0.37594047175202405 1106 68-69 0.25836861760279106 1106 70-71 0.22044241250929275 1106 72-73 0.20833223899821718 1106 74-75 0.28036622209200246 1106 76-77 0.47757865122243714 1106 78-79 0.47171436070461326 1106 80-81 0.4978072882664719 1106 82-83 0.610476497220855 1106 84-85 0.5294174357012054 1106 86-87 0.46910530176765164 1106 88-89 0.5060991391792946 1106 90-91 0.37870607383984733 1106 92-93 0.4793656664815025 1106 94-95 0.24977745345610813 1106 96-97 0.12323295147935553 1106 98-99 -0.030851718955631213 1106 100-101 -0.03564051775383703 1107 1 0.1342135575709591 1107 2 0.07285521120638094 1107 3 0.12896890843656905 1107 4 0.10931011351769371 1107 5 0.06139612201733513 1107 6 0.04506097475648829 1107 7 0.0451271520089378 1107 8 0.12830352046338334 1107 9 0.05117109303947842 1107 10-11 0.19613367951966865 1107 12-13 0.11131757615845572 1107 14-15 0.12828489005644172 1107 16-17 0.09495311949502394 1107 18-19 0.12394182254214314 1107 20-21 0.08092882600966789 1107 22-23 0.02266154157938871 1107 24-25 0.02916323450892122 1107 26-27 -0.018354495216918565 1107 28-29 0.11653811603783026 1107 30-31 0.12617006507593942 1107 32-33 0.25802704513407804 1107 34-35 0.20431189463924682 1107 36-37 0.15573574358913334 1107 38-39 0.2439469781817465 1107 40-41 0.2990728464798096 1107 42-43 0.2147241583150148 1107 44-45 0.23048865465136004 1107 46-47 0.2108919717286426 1107 48-49 0.20423480045615605 1107 50-51 0.09713992383672831 1107 52-53 0.1677751346912828 1107 54-55 0.5261148815487218 1107 56-57 0.3406081124978826 1107 58-59 0.3197048576151502 1107 60-61 0.3652035808305456 1107 62-63 0.41699331040229737 1107 64-65 0.33969890419769655 1107 66-67 0.3644123018087555 1107 68-69 0.4850885014924913 1107 70-71 0.23559216841376696 1107 72-73 0.1066713338656129 1107 74-75 0.194956537437033 1107 76-77 0.28887037836322094 1107 78-79 0.39714251745352414 1107 80-81 0.6333412637249722 1107 82-83 0.6612478390865348 1107 84-85 0.6039853206464478 1107 86-87 0.4158093524339641 1107 88-89 0.27398751495446305 1107 90-91 0.44617091088958816 1107 92-93 0.1373524721286863 1107 94-95 0.34788564714920867 1107 96-97 0.48905667492572036 1107 98-99 0.005943108858069479 1107 100-101 0.19184806913097674 1108 1 0.06524551968679759 1108 2 -0.01621177469299795 1108 3 0.011658302777256324 1108 4 0.019731437132790575 1108 5 0.10177517554884474 1108 6 0.09734769093248019 1108 7 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-1.3368906729750094 2316 58-59 -1.2613165861662452 2316 60-61 -1.3802739137173248 2316 62-63 -1.433979673369194 2316 64-65 -1.2335170273710716 2316 66-67 -1.1184980532266522 2316 68-69 -1.5781694428768738 2316 70-71 -1.2896609148203098 2316 72-73 -1.1712608977920027 2316 74-75 -1.2288687764860633 2316 76-77 -1.1899083143667184 2316 78-79 -1.124510873914783 2316 80-81 -1.2083381283606975 2316 82-83 -1.1026170661500814 2316 84-85 -1.2082922998187478 2316 86-87 -1.0905761846291675 2316 88-89 -1.2036595109297181 2316 90-91 -1.1035959453088857 2316 92-93 -1.1565816271008238 2316 94-95 -1.2843990561656575 2316 96-97 -0.9301969470892288 2316 98-99 -1.1558296315539778 2316 100-101 -1.560797783845409 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 2.0 4 6.0 5 28.0 6 53.0 7 132.0 8 319.0 9 652.0 10 1130.0 11 1863.0 12 2857.0 13 3952.0 14 5163.0 15 7283.0 16 9946.0 17 13754.0 18 12337.0 19 7275.0 20 6286.0 21 6484.0 22 6706.0 23 7225.0 24 8268.0 25 10036.0 26 12503.0 27 15227.0 28 19066.0 29 23702.0 30 29031.0 31 36596.0 32 46876.0 33 61045.0 34 83779.0 35 119786.0 36 180451.0 37 259312.0 38 168129.0 39 9749.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.21390541118202 19.91046092736168 15.223511995057335 21.65212166639897 2 15.514266123680805 21.47450739156922 39.84318776769427 23.16803871705571 3 19.17383879491253 26.2271348586673 30.261085292393435 24.33794105402673 4 12.865535830349065 17.781147913560265 37.565515864418586 31.787800391672082 5 13.243229236140081 38.39486359067772 33.822001361077106 14.539905812105088 6 30.43313140924886 37.43765983296659 17.565951011461244 14.56325774632331 7 27.584217636213797 33.872439486495445 21.028793298272742 17.51454957901802 8 26.45601991486903 34.693418067815905 19.793629620818855 19.056932396496208 9 26.949133822142546 16.735711676196463 20.695236234186627 35.61991826747437 10-11 24.580996092596582 27.442998091970345 27.186639798754065 20.789366016679008 12-13 26.10253948564583 24.89702721302283 27.978776730868898 21.021656570462444 14-15 22.992880264398774 25.788438500947315 27.01922668456513 24.199454550088785 16-17 21.45844130466181 29.327108520743238 27.42007289657692 21.79437727801803 18-19 21.38493771484597 27.259971486976276 30.671593857635386 20.68349694054236 20-21 22.384353081163656 26.335547581411532 30.762910912800656 20.517188424624162 22-23 21.983028706761967 26.317186886011957 30.66527894306439 21.034505464161686 24-25 21.888193026201137 26.493816470972103 30.78109725192066 20.836893250906105 26-27 21.785170958090063 26.596416726249117 30.675127587163498 20.943284728497318 28-29 21.320000084961226 26.627485381359083 31.047168348544936 21.00534618513476 30-31 21.495149235819316 26.59026771494321 30.86816720257235 21.046415846665127 32-33 21.420539244486758 26.790765883166102 30.672456150859652 21.11623872148749 34-35 21.88260932362512 26.625219836704872 30.50844937596112 20.98372146370889 36-37 21.91903502749587 26.46545512633146 30.462892470413898 21.15261737575877 38-39 21.723663548733565 26.70671998422906 30.566734191671312 21.002882275366062 40-41 21.707965996500413 26.595533631578345 30.616021373045715 21.080478998875527 42-43 21.675474295035727 26.767359665593325 30.202929456844036 21.354236582526912 44-45 21.810647318204605 26.985114824852808 29.820349869584795 21.383887987357795 46-47 21.999529314609003 27.21874211453288 29.202290215776117 21.579438355082 48-49 22.159150769941853 27.38871442990997 28.890279462892916 21.561855337255263 50-51 22.21301745987644 27.50616648271303 28.67633251680011 21.60448354061042 52-53 22.352104060288358 27.661617148537392 28.619935259683437 21.366343531490813 54-55 22.036472420954965 27.53350442215466 28.638628887785305 21.791394269105073 56-57 22.158178270549918 28.03755747281472 28.313350515879975 21.49091374075539 58-59 22.297554477114854 27.628444326631595 28.46514212307818 21.608859073175374 60-61 22.274743200876813 27.510938920466614 28.729258532357964 21.48505934629861 62-63 22.262868610726173 27.57756681872598 28.827616735757505 21.331947834790345 64-65 22.118045776181074 27.511417707333713 28.69148464816979 21.679051868315423 66-67 22.04172785769724 27.816235316807752 28.36458624950987 21.777450575985135 68-69 21.97846825824666 27.789821041769947 28.524268982371805 21.707441717611587 70-71 22.124492672478613 27.74662043617635 28.412847567674117 21.716039323670923 72-73 22.23039736280915 27.703333021809502 28.17278527795006 21.89348433743129 74-75 22.124281017153635 28.052522918241984 27.99878505705134 21.82441100755304 76-77 22.163150695406156 27.908981232105077 28.11658354644396 21.81128452604481 78-79 22.202614726154696 27.79158657165008 28.09345893647978 21.912339765715437 80-81 22.140652958159087 28.3033215461239 27.812150058369628 21.743875437347384 82-83 22.30094729243963 28.173973483289082 27.76488352045938 21.7601957038119 84-85 22.144344683024066 27.99173095174774 27.671360560922203 22.19256380430599 86-87 22.183634108210985 27.91377786840674 27.881023994018488 22.021564029363784 88-89 22.338666203345177 27.70910320528696 27.785568243559837 22.16666234780802 90-91 22.07611195553452 27.881704634567626 28.05260479370061 21.98957861619724 92-93 22.16402855204529 27.828048820378104 27.886544574656853 22.121378052919756 94-95 21.97260173881423 27.78449442612588 27.805309899924303 22.437593935135585 96-97 22.021016374862253 27.702302574285014 27.88314569319797 22.393535357654756 98-99 21.894800250979298 27.76120772530256 27.569659712342375 22.774332311375762 100-101 21.841874414564053 28.02114684910674 27.544769255930824 22.592209480398385 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 794.0 1 563.0 2 384.5 3 437.0 4 397.0 5 370.0 6 629.0 7 1307.5 8 2165.5 9 2628.0 10 2333.0 11 1877.0 12 1871.0 13 1989.5 14 2092.0 15 2249.0 16 2337.0 17 2459.0 18 2565.5 19 2628.0 20 2816.5 21 3216.0 22 3754.5 23 4375.0 24 5232.5 25 6276.5 26 7661.0 27 9305.5 28 10677.0 29 12333.5 30 14537.5 31 16637.5 32 19087.0 33 22066.0 34 25394.0 35 27955.0 36 30905.5 37 33890.0 38 35111.0 39 35764.5 40 35946.0 41 37134.0 42 38418.5 43 39518.5 44 41180.5 45 41887.0 46 41578.5 47 42156.0 48 46903.5 49 47171.0 50 42504.5 51 39867.5 52 37617.5 53 36495.5 54 35281.5 55 32876.5 56 29568.0 57 25931.0 58 23367.0 59 21417.0 60 18654.0 61 15283.0 62 12192.0 63 9601.0 64 7160.5 65 5281.0 66 3876.5 67 3030.5 68 2097.5 69 1215.5 70 873.0 71 643.5 72 477.0 73 369.0 74 273.0 75 187.5 76 120.5 77 68.5 78 41.5 79 27.5 80 14.0 81 10.0 82 6.5 83 2.0 84 1.5 85 1.0 86 1.5 87 2.0 88 1.5 89 1.5 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.026932651379342573 2 0.005182623767002829 3 0.0 4 4.2480522680351065E-4 5 8.496104536070212E-5 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.01202198791853935 12-13 0.0 14-15 0.0 16-17 0.0 18-19 3.398441814428085E-4 20-21 9.770520216480743E-4 22-23 0.0019116235206157977 24-25 0.011342299555653733 26-27 0.0059047926525687975 28-29 2.1240261340175532E-4 30-31 0.0025913118835014146 32-33 0.0041206106999940526 34-35 0.0 36-37 0.009005870808234425 38-39 0.013763689348433744 40-41 0.0011469741123694786 42-43 0.0 44-45 0.0 46-47 2.548831360821064E-4 48-49 0.003228519723706681 50-51 0.007051766764938276 52-53 0.0 54-55 0.003271000246387032 56-57 0.003440922337108436 58-59 1.6992209072140424E-4 60-61 0.0016992209072140426 62-63 0.004842779585560021 64-65 4.248052268035106E-5 66-67 0.002251467702058606 68-69 2.9736365876245743E-4 70-71 7.646494082463192E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0019116235206157977 80-81 0.0023789092700996595 82-83 0.011002455374210924 84-85 0.007731455127823893 86-87 0.004248052268035106 88-89 1.274415680410532E-4 90-91 5.947273175249149E-4 92-93 1.274415680410532E-4 94-95 8.496104536070212E-5 96-97 0.0019541040432961487 98-99 0.0017417014298943935 100-101 2.1240261340175532E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1177010.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.575859983149236 #Duplication Level Percentage of deduplicated Percentage of total 1 79.47324022600395 46.55213391885591 2 12.22644531767196 14.323490980391664 3 3.549814693655249 6.238003453850269 4 1.512489109075998 3.5438140111709515 5 0.7790193139329543 2.2815863128552856 6 0.4993290152059108 1.7549175888135131 7 0.3114005996270029 1.2768390545694026 8 0.26150849913866897 1.2254468183960139 9 0.17988275356086636 0.9483108287368182 >10 1.0752859488301454 11.866945390187073 >50 0.09012175121963598 3.594451256098514 >100 0.03943312888643859 4.0601172334785804 >500 0.001014821595635595 0.398444959684907 >1k 7.248725683111394E-4 0.7765091030176449 >5k 2.899490273244557E-4 1.1589890898933526 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7645 0.6495271917825677 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6003 0.5100211553002948 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4153 0.3528432213829959 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1460 0.1240431262266251 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1250 0.10620130670087764 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 2.9736365876245743E-4 0.0 24-25 0.0 0.0 0.0 5.097662721642128E-4 0.0 26-27 0.0 0.0 0.0 0.0012319351577301809 0.0 28-29 0.0 0.0 0.0 0.002251467702058606 0.0 30-31 0.0 0.0 0.0 0.004248052268035106 0.0 32-33 0.0 0.0 0.0 0.005947273175249148 0.0 34-35 0.0 0.0 0.0 0.00760401355978284 0.0 36-37 0.0 0.0 0.0 0.01049268910204671 0.0 38-39 0.0 0.0 0.0 0.012361832099982158 0.0 40-41 0.0 0.0 0.0 0.01563283234636919 0.0 42-43 0.0 0.0 0.0 0.019583520955641837 0.0 44-45 0.0 0.0 0.0 0.027102573470063975 0.0 46-47 0.0 0.0 0.0 0.043627496792720544 0.0 48-49 0.0 0.0 0.0 0.08202988929575789 0.0 50-51 0.0 0.0 0.0 0.10781556656273099 0.0 52-53 0.0 0.0 0.0 0.13181706187712933 0.0 54-55 0.0 0.0 0.0 0.1642297006822372 0.0 56-57 0.0 0.0 0.0 0.18831615704199622 0.0 58-59 0.0 0.0 0.0 0.21529128894401917 0.0 60-61 0.0 0.0 0.0 0.26537582518415304 0.0 62-63 0.0 0.0 0.0 0.30156923050781215 0.0 64-65 0.0 0.0 0.0 0.3351288434252895 0.0 66-67 0.0 0.0 0.0 0.4028852771004494 0.0 68-69 0.0 0.0 0.0 0.48334338705703433 0.0 70-71 0.0 0.0 0.0 0.5478288204858073 0.0 72-73 0.0 0.0 0.0 0.6289241382825974 0.0 74-75 0.0 0.0 0.0 0.7050917154484668 0.0 76-77 0.0 0.0 0.0 0.7602314338875626 0.0 78-79 0.0 0.0 0.0 0.8301543742194204 0.0 80-81 0.0 0.0 0.0 0.9522433964027494 0.0 82-83 0.0 0.0 0.0 1.0622254696221782 0.0 84-85 0.0 0.0 0.0 1.1953594277023984 0.0 86-87 0.0 0.0 0.0 1.447736212946364 0.0 88-89 0.0 0.0 0.0 1.7480310277737656 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6305 0.0 31.875946 1 GTATCAA 9145 0.0 28.834812 1 GTACATG 6140 0.0 24.065775 1 TATCAAC 11085 0.0 23.652773 2 ATCAACG 11095 0.0 23.500025 3 TCAACGC 11125 0.0 23.436657 4 GAGTACT 6305 0.0 23.162388 12-13 CAACGCA 11290 0.0 23.102968 5 TACATGG 6315 0.0 22.940619 2 AACGCAG 11575 0.0 22.616217 6 ACATGGG 6260 0.0 22.304695 3 GTACTTT 6590 0.0 21.801268 14-15 AGTACTT 6575 0.0 21.01941 12-13 ACGCAGA 12560 0.0 20.65344 7 CGCAGAG 12610 0.0 20.458515 8 CATGGGG 2370 0.0 20.03895 4 GTCCGCG 145 6.137037E-4 19.659506 8 GGTCCGC 170 7.940596E-5 19.563137 7 GCAGAGT 13125 0.0 19.402374 9 TGTACGG 125 0.006023549 19.00419 5 >>END_MODULE