FastQCFastQC Report
Thu 26 May 2016
SRR936968_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936968_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1187220
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75350.6346759657013865No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52860.4452418254409461No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36350.30617745657923556No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12970.10924681187985377No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA82900.033.88671
GGTATCA53550.031.1561621
ATCAACG99500.027.995563
TATCAAC100200.027.8474222
TCAACGC100150.027.813864
CAACGCA100750.027.6447245
AACGCAG102600.027.0976476
GTACATG63100.025.386151
ACGCAGA112000.024.6887957
CGCAGAG112200.024.4754058
TACATGG65400.024.2036632
ACATGGG63100.023.8052273
GAGTACT54950.023.56199512-13
GCAGAGT114700.023.280179
CATGGGG24200.022.5890054
AGTACTT57700.021.57439812-13
GTACTTT60050.021.28351614-15
TACACTG9450.020.1181415
CTCGCGC1452.4501787E-919.65023660-61
AGAGTAC103100.019.56285710-11
Produced by FastQC (version 0.11.5)