##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936968_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1187220 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.684278398274962 33.0 31.0 34.0 30.0 34.0 2 31.765666009669648 33.0 31.0 34.0 30.0 34.0 3 31.78190057445124 33.0 31.0 34.0 30.0 34.0 4 35.39399521571402 37.0 35.0 37.0 33.0 37.0 5 35.39607149475245 37.0 35.0 37.0 33.0 37.0 6 35.465375414834654 37.0 35.0 37.0 33.0 37.0 7 35.40742743552164 37.0 35.0 37.0 33.0 37.0 8 35.36552113340409 37.0 35.0 37.0 33.0 37.0 9 36.96971917588989 39.0 37.0 39.0 33.0 39.0 10-11 36.83737723421101 39.0 37.0 39.0 33.0 39.0 12-13 36.75067805461498 39.0 37.0 39.0 32.0 39.0 14-15 37.85729308805445 40.0 37.5 41.0 32.5 41.0 16-17 37.790390576304304 40.0 37.0 41.0 32.5 41.0 18-19 37.76805351998787 40.0 37.5 41.0 32.0 41.0 20-21 37.71460386449015 40.0 37.0 41.0 32.0 41.0 22-23 37.60230538569094 40.0 37.0 41.0 32.0 41.0 24-25 37.55626674078941 40.0 37.0 41.0 32.0 41.0 26-27 37.57237622344637 40.0 37.0 41.0 32.0 41.0 28-29 37.48020754367346 40.0 37.0 41.0 32.0 41.0 30-31 37.30264314954263 40.0 37.0 41.0 31.5 41.0 32-33 37.08881588922019 39.0 36.0 41.0 31.0 41.0 34-35 36.90625284277556 39.0 36.0 41.0 30.5 41.0 36-37 36.71537962635401 39.0 35.5 41.0 30.0 41.0 38-39 36.54371346506966 39.0 35.0 41.0 30.0 41.0 40-41 36.34947524468927 39.0 35.0 41.0 30.0 41.0 42-43 36.1127440575462 39.0 35.0 40.5 30.0 41.0 44-45 35.83421185626927 39.0 35.0 40.0 28.5 41.0 46-47 35.45210239045838 38.5 35.0 40.0 26.5 41.0 48-49 35.17254299961254 38.5 35.0 40.0 26.0 41.0 50-51 34.463572463401896 38.0 34.0 39.5 25.0 40.5 52-53 34.64001364532269 38.0 34.0 40.0 25.5 40.5 54-55 35.025690689173025 39.0 35.0 40.0 26.0 41.0 56-57 34.91884317986557 38.5 34.5 40.0 25.5 41.0 58-59 34.69769545661293 38.0 34.0 40.0 25.0 41.0 60-61 34.41585215882482 38.0 34.0 40.0 24.0 41.0 62-63 34.10181263792726 37.5 34.0 40.0 23.0 41.0 64-65 33.77030415592729 37.0 33.5 40.0 22.5 41.0 66-67 33.3994525024848 36.5 33.0 39.5 22.0 41.0 68-69 33.006433095803644 36.0 33.0 39.0 20.5 41.0 70-71 32.58977948484695 35.5 32.0 39.0 20.0 40.5 72-73 32.20108572968785 35.0 32.0 38.0 18.5 40.0 74-75 31.812280369266016 35.0 31.5 37.0 18.0 39.5 76-77 31.360471100554236 35.0 31.0 37.0 14.0 39.0 78-79 30.898648944593255 35.0 31.0 36.0 9.5 39.0 80-81 30.469785717895586 35.0 30.5 36.0 7.0 37.5 82-83 30.0640508919998 34.0 30.0 35.5 4.5 37.0 84-85 29.69508599922508 34.0 30.0 35.0 2.0 37.0 86-87 29.33740924175806 34.0 29.5 35.0 2.0 36.0 88-89 28.96713372416233 34.0 29.0 35.0 2.0 36.0 90-91 28.586145364801805 34.0 29.0 35.0 2.0 35.5 92-93 28.195045568639344 34.0 29.0 35.0 2.0 35.0 94-95 27.810530904128974 34.0 27.5 35.0 2.0 35.0 96-97 27.31430063509712 34.0 27.0 35.0 2.0 35.0 98-99 26.73442748605987 34.0 25.5 35.0 2.0 35.0 100-101 24.572089840130726 31.0 20.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.31494848181039714 1101 2 0.21805534952825667 1101 3 0.23401492603479923 1101 4 0.25062625837288977 1101 5 0.32946399460811904 1101 6 0.18319889292928337 1101 7 0.19733821930085327 1101 8 0.24136646327302458 1101 9 0.24065306816763155 1101 10-11 0.35008790349411356 1101 12-13 0.4350648752976909 1101 14-15 0.522944301511636 1101 16-17 0.5313279378951066 1101 18-19 0.4048050098278182 1101 20-21 0.5492812348433844 1101 22-23 0.5030345734845696 1101 24-25 0.49678014324972963 1101 26-27 0.5224600664919308 1101 28-29 0.4855718403160054 1101 30-31 0.49414380626683396 1101 32-33 0.38741589776256546 1101 34-35 0.5609875650417351 1101 36-37 0.5066204855439693 1101 38-39 0.42470576934932325 1101 40-41 0.3374785181769582 1101 42-43 0.3665335115150796 1101 44-45 0.7187175905798071 1101 46-47 0.5226691395593974 1101 48-49 0.49508568837176625 1101 50-51 0.49098015695668806 1101 52-53 0.5035846978685043 1101 54-55 0.6124891769811569 1101 56-57 0.5733658355020239 1101 58-59 0.6339379226366404 1101 60-61 0.6687368327678129 1101 62-63 0.7580129854277331 1101 64-65 0.574589734556092 1101 66-67 0.7633329518679588 1101 68-69 0.5919776938095467 1101 70-71 0.4225611519758843 1101 72-73 0.6596967939550211 1101 74-75 0.6851715670286467 1101 76-77 0.5670237777515013 1101 78-79 0.6484725956470747 1101 80-81 0.6387207223637219 1101 82-83 0.6010028806325067 1101 84-85 0.4259728907688185 1101 86-87 0.6073512965953505 1101 88-89 0.5834817180607885 1101 90-91 0.8132235052495993 1101 92-93 0.7917646248206829 1101 94-95 0.6785647897278189 1101 96-97 0.8604458176604375 1101 98-99 0.7013926064510407 1101 100-101 0.7266487397037302 1102 1 0.13336580170587808 1102 2 -0.0012802234467343965 1102 3 -0.04882195339782314 1102 4 0.011484787674866936 1102 5 0.09463010136437333 1102 6 -0.006274791281242642 1102 7 0.027625639980215055 1102 8 -0.11464697457691386 1102 9 0.07123910773463393 1102 10-11 0.02793784750306827 1102 12-13 -0.014614109398102926 1102 14-15 -0.14292355963058156 1102 16-17 0.07480311520007632 1102 18-19 -0.2275242174958052 1102 20-21 -0.14681059046456113 1102 22-23 -0.07231817007646413 1102 24-25 -0.20482493327136098 1102 26-27 -0.05314277038750248 1102 28-29 -0.3263318550927252 1102 30-31 -0.07327314035503463 1102 32-33 -0.11512982825460227 1102 34-35 -0.25143871342037016 1102 36-37 -0.14672404599765798 1102 38-39 -0.1758354773845312 1102 40-41 -0.22566071287941014 1102 42-43 -0.016855812859681407 1102 44-45 -0.08722492528432468 1102 46-47 -0.2054383632401553 1102 48-49 -0.05208116493170678 1102 50-51 -0.09330875681710182 1102 52-53 -0.11872213303855261 1102 54-55 -0.3283842832652084 1102 56-57 -0.2876569341881563 1102 58-59 -0.5417806290617548 1102 60-61 -0.5649511104945972 1102 62-63 -0.7094935468604291 1102 64-65 -0.3561194853020666 1102 66-67 -0.6537928413384648 1102 68-69 -0.45075466901240446 1102 70-71 -0.5629558319733476 1102 72-73 -0.5528750612148201 1102 74-75 -0.2401401164353274 1102 76-77 -0.2346708844358787 1102 78-79 -0.3179365122290001 1102 80-81 -0.23755773974900762 1102 82-83 -0.13000122537719605 1102 84-85 -0.23767399239653741 1102 86-87 -0.08836352236695078 1102 88-89 0.08791247580621686 1102 90-91 -0.034294225246853216 1102 92-93 -0.057115554350652076 1102 94-95 -0.022166826546911267 1102 96-97 -0.07272700802078802 1102 98-99 -0.0065133285993503875 1102 100-101 -0.01603883028507269 1103 1 0.2354182633328108 1103 2 0.19831577189394167 1103 3 0.02677999879987425 1103 4 0.1116292856917056 1103 5 -0.022484066813625248 1103 6 0.09684371183725915 1103 7 0.20340452852505564 1103 8 0.20697612151426625 1103 9 0.14810173140576666 1103 10-11 0.21036364482248615 1103 12-13 0.1230460548578165 1103 14-15 0.32477965913647466 1103 16-17 0.3324644590316268 1103 18-19 0.29205452655101993 1103 20-21 0.12966056153850047 1103 22-23 0.30670528873422853 1103 24-25 0.24031152888637308 1103 26-27 0.34894655297841837 1103 28-29 0.3754824797003309 1103 30-31 0.29592445331064 1103 32-33 0.3784119950744511 1103 34-35 0.4254541368767306 1103 36-37 0.4995927290425328 1103 38-39 0.5003230870719051 1103 40-41 0.4654976304065954 1103 42-43 0.6356913333044858 1103 44-45 0.6390604435542429 1103 46-47 0.6713579093008022 1103 48-49 0.8487976189258006 1103 50-51 0.8363833739388582 1103 52-53 0.7973776000824557 1103 54-55 0.9376159231605357 1103 56-57 0.9818554587284964 1103 58-59 0.932354963158943 1103 60-61 0.9723404363714181 1103 62-63 0.9773622942507316 1103 64-65 1.0147560547224046 1103 66-67 1.0367056768196292 1103 68-69 1.0603097505626522 1103 70-71 0.9211022031485143 1103 72-73 0.7019501404188944 1103 74-75 0.7347400829129498 1103 76-77 0.7308958279393423 1103 78-79 0.8309841760533914 1103 80-81 0.6890070410710933 1103 82-83 0.7864214165773618 1103 84-85 0.7684472679800436 1103 86-87 0.6890903635975754 1103 88-89 0.5509128739129956 1103 90-91 0.7464875385136338 1103 92-93 0.790243670668147 1103 94-95 0.9629292351448946 1103 96-97 0.8438334968375898 1103 98-99 1.0220279786653599 1103 100-101 0.9672082465790233 1104 1 0.1679527767801865 1104 2 0.05122853144570527 1104 3 0.08264563142899206 1104 4 0.21235880550836583 1104 5 0.13870473130462813 1104 6 0.15734899848362716 1104 7 -0.11994211847304115 1104 8 -0.00844645075478212 1104 9 0.21853906947795565 1104 10-11 0.3041157116036004 1104 12-13 0.09997933988238117 1104 14-15 -0.08582253258605732 1104 16-17 0.2501246183930377 1104 18-19 0.189395531775844 1104 20-21 0.2577772674560421 1104 22-23 0.1532049163541913 1104 24-25 -0.020115831126041428 1104 26-27 0.07407832375334067 1104 28-29 0.20133801924882277 1104 30-31 0.15312102108811843 1104 32-33 -0.07114201153349597 1104 34-35 -0.045631056200157616 1104 36-37 -0.0490488745783324 1104 38-39 -0.0023561801301781315 1104 40-41 -0.16982657027030967 1104 42-43 -0.03269848964965405 1104 44-45 -0.19260293064025547 1104 46-47 -0.1139254589701224 1104 48-49 -0.06486772212542746 1104 50-51 -0.10653967998005243 1104 52-53 -0.022048992165714765 1104 54-55 0.20148240694403086 1104 56-57 0.08364173271393582 1104 58-59 -0.03171323846112983 1104 60-61 0.03367459210391388 1104 62-63 -0.15771461157306277 1104 64-65 -0.14877126129453444 1104 66-67 -0.4387373691628298 1104 68-69 -0.35106955085814917 1104 70-71 -0.3144993415816586 1104 72-73 -0.2571656942156153 1104 74-75 -0.2734278360433464 1104 76-77 -0.10514409005078207 1104 78-79 -0.29544747423717865 1104 80-81 -0.21600010459995644 1104 82-83 -0.12658902444137965 1104 84-85 -0.1209572977730744 1104 86-87 0.16713072441801913 1104 88-89 -0.12035390834963522 1104 90-91 -0.05302822365772997 1104 92-93 0.014578921974351289 1104 94-95 -0.004599656657855888 1104 96-97 -0.13520898324223296 1104 98-99 0.025186739085530263 1104 100-101 0.062101895414581776 1105 1 0.2575752389781556 1105 2 0.19444346569605386 1105 3 0.24576968290583778 1105 4 0.08538609665782104 1105 5 0.14690845141610254 1105 6 -0.008811789167076256 1105 7 0.04235732096622513 1105 8 0.09493124202752767 1105 9 -0.032179541898763375 1105 10-11 0.24523051689865838 1105 12-13 0.3082721005489759 1105 14-15 0.3673068993381321 1105 16-17 0.3370502775709312 1105 18-19 0.27553532398530933 1105 20-21 0.19868518870266172 1105 22-23 0.17259134066679138 1105 24-25 0.0776080057177424 1105 26-27 0.3261669022917886 1105 28-29 0.43855726370534853 1105 30-31 0.39067435038611364 1105 32-33 0.2127606550045087 1105 34-35 0.11432905830979223 1105 36-37 0.13503041099183122 1105 38-39 0.42949416740577817 1105 40-41 0.29737243902558674 1105 42-43 0.40555283572412293 1105 44-45 0.21001316566975703 1105 46-47 0.18204008277367478 1105 48-49 0.3912787079185165 1105 50-51 0.1046947817851489 1105 52-53 0.28760086937549545 1105 54-55 0.2012470123730168 1105 56-57 0.2764547416998724 1105 58-59 0.3913690268241723 1105 60-61 0.41137249168254186 1105 62-63 0.20891243277761617 1105 64-65 0.07901538642359895 1105 66-67 0.11100106506851404 1105 68-69 0.2645632803975744 1105 70-71 0.13670380247104674 1105 72-73 0.2012820467275418 1105 74-75 0.24847263152922494 1105 76-77 0.04295345395046013 1105 78-79 0.15308518420091488 1105 80-81 0.14152477265859176 1105 82-83 0.23371160805359636 1105 84-85 0.18326898512734147 1105 86-87 0.3744526652389446 1105 88-89 0.4865817514423405 1105 90-91 0.3753094812890616 1105 92-93 0.38072274951931817 1105 94-95 0.5065333368885341 1105 96-97 0.4742516853953127 1105 98-99 0.4268619881505309 1105 100-101 0.585791946558075 1106 1 0.31525887317341983 1106 2 0.3376504912286613 1106 3 0.19965595167582606 1106 4 0.3001809142433345 1106 5 0.19359354926398709 1106 6 0.27295597794952897 1106 7 0.3282693933899239 1106 8 0.3043219288600696 1106 9 0.3512063745104115 1106 10-11 0.30180855126739914 1106 12-13 0.29014584695760703 1106 14-15 0.25401042836724486 1106 16-17 0.26402024558741743 1106 18-19 0.3173421218386139 1106 20-21 0.3030666599445979 1106 22-23 0.16619246822141065 1106 24-25 0.16188810546295684 1106 26-27 0.16643442546629217 1106 28-29 0.1829807471985987 1106 30-31 0.26684960923579837 1106 32-33 0.2636372202996782 1106 34-35 0.3361090225316232 1106 36-37 0.1980577325075359 1106 38-39 0.18801885976768062 1106 40-41 0.29154059644955765 1106 42-43 0.2298735924867472 1106 44-45 0.06077562026941763 1106 46-47 0.23631286425575837 1106 48-49 0.30671861684679413 1106 50-51 0.42388164143712714 1106 52-53 0.3958114185162742 1106 54-55 0.49356879911341167 1106 56-57 0.5202754171484543 1106 58-59 0.36853306433704347 1106 60-61 0.398993243024222 1106 62-63 0.41272958461802034 1106 64-65 0.1947179396842884 1106 66-67 0.13533700045095287 1106 68-69 0.4130505683034684 1106 70-71 0.29436277140908373 1106 72-73 0.2869640004327536 1106 74-75 0.5339569921298661 1106 76-77 0.2831168951604113 1106 78-79 0.2458005308697473 1106 80-81 0.4122294942935447 1106 82-83 0.4456452558011996 1106 84-85 0.5560876006203728 1106 86-87 0.8258263303335376 1106 88-89 0.6996827976829216 1106 90-91 0.7650183770444734 1106 92-93 0.7279117233362022 1106 94-95 0.8075931298087902 1106 96-97 1.0667683547724458 1106 98-99 1.1813453729827543 1106 100-101 1.2050495494203268 1107 1 0.4523556869741405 1107 2 0.41160346562856276 1107 3 0.37354139584060064 1107 4 0.3304565603849028 1107 5 0.3257440802971985 1107 6 0.3566940834194767 1107 7 0.4306611915359113 1107 8 0.4464540425564323 1107 9 0.45644058164405976 1107 10-11 0.5809561355267121 1107 12-13 0.7041445577440228 1107 14-15 0.48249345372178 1107 16-17 0.7087740671848124 1107 18-19 0.7788947033353324 1107 20-21 0.7821522391698394 1107 22-23 0.7347958201767213 1107 24-25 0.7547602891451888 1107 26-27 0.7761339159199707 1107 28-29 0.7940643005167871 1107 30-31 0.7620896485261142 1107 32-33 0.5833781497612804 1107 34-35 0.7268497064287303 1107 36-37 0.6530362456145298 1107 38-39 0.7916510655507238 1107 40-41 0.8058729679886341 1107 42-43 0.9075508593539539 1107 44-45 0.9385271703952682 1107 46-47 0.9441040116558455 1107 48-49 0.8587512769241528 1107 50-51 0.9525525236275527 1107 52-53 0.9840660688938314 1107 54-55 0.9752116255607035 1107 56-57 1.073033720297822 1107 58-59 1.0637988878989617 1107 60-61 1.04285228900617 1107 62-63 1.1474950705287554 1107 64-65 1.2475293461549128 1107 66-67 1.2559720916167763 1107 68-69 1.136752759966555 1107 70-71 0.964376584411724 1107 72-73 1.0170563940726325 1107 74-75 1.1665182297749013 1107 76-77 1.1519139361082864 1107 78-79 1.18755017787079 1107 80-81 1.2623412261371207 1107 82-83 1.3013159538182677 1107 84-85 1.1198732842105272 1107 86-87 1.1894072745290103 1107 88-89 1.1881397318102458 1107 90-91 1.308727628686686 1107 92-93 1.2674006422385276 1107 94-95 1.2491457113055056 1107 96-97 1.0664009289022296 1107 98-99 1.2729357100200183 1107 100-101 1.3912356259136693 1108 1 0.009555600944533893 1108 2 -0.01399247604905085 1108 3 0.02602680428275761 1108 4 0.1034954022794281 1108 5 0.09516399070079729 1108 6 -0.09298600137775281 1108 7 -0.036464773705041864 1108 8 -0.04392717170528471 1108 9 -0.031762045339974065 1108 10-11 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-1.9035386864583117 2316 64-65 -2.485192735053637 2316 66-67 -2.1030101130880965 2316 68-69 -2.13638173643594 2316 70-71 -1.758164173814599 2316 72-73 -2.378350483547756 2316 74-75 -2.5212947426996024 2316 76-77 -2.6650087507867326 2316 78-79 -3.0530891782694844 2316 80-81 -2.7757167629041533 2316 82-83 -3.200318128223607 2316 84-85 -3.0101260417832876 2316 86-87 -3.4207276339363553 2316 88-89 -3.3949097019479666 2316 90-91 -3.8152473840658168 2316 92-93 -3.8152899598174983 2316 94-95 -4.0652992369415415 2316 96-97 -4.3235158619705025 2316 98-99 -4.698411750891449 2316 100-101 -4.656017186147366 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2287.0 3 874.0 4 874.0 5 879.0 6 1127.0 7 1268.0 8 1555.0 9 2011.0 10 2503.0 11 3326.0 12 4395.0 13 5471.0 14 6213.0 15 7771.0 16 9740.0 17 12948.0 18 11291.0 19 7046.0 20 6912.0 21 7143.0 22 7859.0 23 8695.0 24 9958.0 25 11343.0 26 13306.0 27 15881.0 28 18621.0 29 22505.0 30 27618.0 31 34071.0 32 42815.0 33 56574.0 34 76442.0 35 107836.0 36 159578.0 37 232709.0 38 208719.0 39 37035.0 40 21.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.06325917199852 19.089823608769255 14.738143329145379 22.10877389008685 2 14.990285143262541 20.589261249601883 41.05340053688696 23.36705307024861 3 18.77339597254367 26.13313809131833 30.07255015784778 25.02091577829022 4 12.29217318023807 17.07750974402191 38.247213736437374 32.38310333930264 5 13.211058059957828 37.900219802085374 34.14368665513837 14.745035482818425 6 30.096428315434853 37.00106674087473 18.320536659203697 14.58196828448672 7 27.452100837503153 33.55625733773956 21.37870700706193 17.61293481769536 8 26.10357223550518 34.16765867328529 20.32048277280744 19.408286318402087 9 26.702395512933442 16.65167662303398 20.880217609915512 35.76571025411706 10-11 24.186886503390696 27.09858513826196 27.706423565013257 21.00810479333409 12-13 25.795124039982415 24.677135281823613 28.16478649394393 21.362954184250043 14-15 22.822484157257108 25.490201036473813 27.166368923822926 24.520945882446153 16-17 21.32495745130023 29.044064819820637 27.518126652067306 22.112851076811832 18-19 21.272521468985122 27.142958282447477 30.613942598225506 20.970577650341895 20-21 22.340857698392163 26.184301572438624 30.55509434884589 20.91974638032332 22-23 21.890852866023337 26.224424235913187 30.400006740858238 21.48471615720524 24-25 21.638517334173482 26.437641917240583 30.582857345031954 21.34098340355398 26-27 21.488259768855315 26.642286732787024 30.444705661364317 21.42474783699334 28-29 21.114574163848257 26.536072140068317 30.83398265572155 21.51537104036188 30-31 21.348728683280616 26.434037678314965 30.716081724795835 21.501151913608584 32-33 21.186157272629252 26.68300009434471 30.488461305191656 21.642381327834386 34-35 21.636717047170386 26.596272951839833 30.281792470511924 21.485217530477854 36-37 21.74778291428841 26.377676170708863 30.233568968886672 21.640971946116057 38-39 21.475961702473555 26.65163663257042 30.39357602921549 21.478825635740538 40-41 21.56429752845826 26.460713980356886 30.518513713916757 21.4564747772681 42-43 21.36419306961551 26.67843406801676 30.07956683833427 21.877806024033465 44-45 21.496429601770565 26.886204217092 29.770487936876282 21.846878244261145 46-47 21.67231089246434 27.012769104486473 29.290015216456517 22.02490478659267 48-49 21.791578698222857 27.203106765368208 29.016804447044365 21.988510089364567 50-51 21.789915060360716 27.24078416706256 28.907484526565113 22.061816246011613 52-53 21.93475971865392 27.390178157772816 28.733858400370636 21.94120372320263 54-55 21.621999565319214 27.274703094489155 28.879357548535147 22.223939791656488 56-57 21.782944691633407 27.714833977185236 28.561879699374792 21.940341631806564 58-59 21.902647226548584 27.353438901182393 28.644622028745747 22.09929184352328 60-61 21.896327855292455 27.285293184899356 28.93359091538918 21.884788044419007 62-63 21.853241914010297 27.322564789633493 28.94959736945517 21.874595926901044 64-65 21.74589650599167 27.245144941056164 28.92406814581834 22.084890407133823 66-67 21.657501153148676 27.57080543485816 28.56287135073201 22.208822061261156 68-69 21.52130320851176 27.610341969619274 28.792460732631326 22.07589408923764 70-71 21.682476538038507 27.4792982692721 28.621317409681456 22.216907783007937 72-73 21.851954180224787 27.401355994450682 28.461647965376567 22.28504185994796 74-75 21.589104104494115 27.78790323090175 28.355906752228734 22.2670859123754 76-77 21.799763172427912 27.50986886863231 28.35544692884515 22.334921030094627 78-79 21.731206943835364 27.600052068654797 28.302684021199866 22.36605696630998 80-81 21.553802844921073 28.00926755231333 28.233791161432347 22.20313844133325 82-83 21.735873855732816 27.87428542541674 28.18282156382831 22.207019155022138 84-85 21.696391867212085 27.672319677599788 27.940410885050575 22.690877570137545 86-87 21.71644680328487 27.6736750796551 28.240687104489208 22.369191012570823 88-89 21.87126972817392 27.404407077177574 28.211125432358653 22.513197762289856 90-91 21.622052061187187 27.510050820792454 28.46474186391218 22.403155254108174 92-93 21.71020310826514 27.45269051248539 28.36841781501247 22.468688564237002 94-95 21.510859213725787 27.420320206018218 28.29380846017474 22.775012120081257 96-97 21.559090461062542 27.43170075626422 28.207130635651158 22.80207814702208 98-99 21.408955933208848 27.395082680005125 28.17275671922504 23.02320466756099 100-101 21.386348932726694 27.425878822490606 28.269624278492056 22.918147966290643 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 524.0 1 367.0 2 295.0 3 393.5 4 355.0 5 348.5 6 600.5 7 1332.5 8 2369.0 9 2747.0 10 2241.0 11 1779.5 12 1794.0 13 1893.5 14 1973.5 15 2090.5 16 2130.5 17 2182.5 18 2288.0 19 2430.5 20 2672.5 21 3035.5 22 3583.0 23 4310.0 24 5112.5 25 6104.0 26 7676.0 27 9405.0 28 10615.0 29 12114.5 30 14604.5 31 17277.0 32 19609.0 33 22280.5 34 25576.5 35 28250.5 36 31372.5 37 34503.0 38 35801.0 39 36445.5 40 36745.5 41 37735.0 42 38787.0 43 39987.5 44 41520.5 45 42206.0 46 41826.0 47 42316.5 48 47418.5 49 47732.5 50 42809.0 51 40287.0 52 38090.5 53 36845.5 54 35746.0 55 33435.0 56 30169.0 57 26574.0 58 23820.0 59 21345.0 60 18414.0 61 15354.5 62 12243.0 63 9457.5 64 7145.5 65 5394.5 66 3931.5 67 2987.0 68 2114.5 69 1297.5 70 906.5 71 667.5 72 506.0 73 370.0 74 260.0 75 190.0 76 139.0 77 72.5 78 35.5 79 22.0 80 17.0 81 14.0 82 10.5 83 10.0 84 7.5 85 4.5 86 8.0 87 6.0 88 2.5 89 4.0 90 3.0 91 1.5 92 1.5 93 1.5 94 0.5 95 0.0 96 0.0 97 1.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04068327689897407 2 0.03183908626876232 3 0.02670103266454406 4 0.05011708023786661 5 0.0971176361584205 6 0.11497447819275282 7 0.060393187446303134 8 0.09029497481511431 9 0.07387004935900676 10-11 0.07454389245464194 12-13 0.07146948333080642 14-15 0.06683681204831456 16-17 0.053781102070382905 18-19 0.06241471673320867 20-21 0.06056164822021193 22-23 0.036008490423005 24-25 0.0108236047236401 26-27 0.0034955610586075033 28-29 0.007159582891123803 30-31 0.005474975152035848 32-33 0.007075352504169404 34-35 0.007370158858509795 36-37 0.006401509408534222 38-39 0.0034955610586075033 40-41 0.007285928471555398 42-43 0.003537676252084702 44-45 0.002316335641245936 46-47 0.001558262158656357 48-49 3.790367412947895E-4 50-51 0.002021529286905544 52-53 0.005896127086807837 54-55 0.01196071494752447 56-57 0.007917656373713382 58-59 0.004464210508583076 60-61 0.0021478748673371406 62-63 0.007538619632418591 64-65 0.002569026802109129 66-67 0.02126817270598541 68-69 0.04881150924007345 70-71 0.061067030541938315 72-73 0.003958828186856691 74-75 0.01410858981486161 76-77 0.023626623540708547 78-79 0.027416990953656437 80-81 0.07837637506106704 82-83 0.027374875760179242 84-85 0.010444567982345311 86-87 0.0323865837839659 88-89 0.0472111318879399 90-91 0.042199423864153235 92-93 0.043210188507606 94-95 0.011834369367092873 96-97 0.01693030777783393 98-99 0.04009366419029329 100-101 0.07601792422634389 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1187220.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.39206937897047 #Duplication Level Percentage of deduplicated Percentage of total 1 80.6110791224033 48.68269883073857 2 11.603172470139677 14.014791936656712 3 3.3436896201507236 6.057970065656577 4 1.410499187400735 3.4073185913794655 5 0.7472109655388341 2.2562808235774394 6 0.4642832410893473 1.6823415424416681 7 0.3209207550821417 1.3566747954250602 8 0.22074698010077481 1.0665093549955365 9 0.15898894503904742 0.8641504259358727 >10 0.9993467440994472 11.456327877769263 >50 0.08393267357874702 3.475455877689297 >100 0.03445535446571836 3.6619212065404416 >500 9.764655317265314E-4 0.4323400183960751 >1k 4.184852278827992E-4 0.5051268024752272 >5k 2.7899015192186616E-4 1.0800918503226964 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7535 0.6346759657013865 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5286 0.4452418254409461 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3635 0.30617745657923556 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1297 0.10924681187985377 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 4.2115193477198834E-5 0.0 22-23 0.0 0.0 0.0 2.948063543403919E-4 0.0 24-25 0.0 0.0 0.0 7.159582891123801E-4 0.0 26-27 0.0 0.0 0.0 8.844190630211755E-4 0.0 28-29 0.0 0.0 0.0 0.002063644480382743 0.0 30-31 0.0 0.0 0.0 0.003790367412947895 0.0 32-33 0.0 0.0 0.0 0.006106703054193831 0.0 34-35 0.0 0.0 0.0 0.007833425986758983 0.0 36-37 0.0 0.0 0.0 0.00972860969323293 0.0 38-39 0.0 0.0 0.0 0.01120264146493489 0.0 40-41 0.0 0.0 0.0 0.014319165782247604 0.0 42-43 0.0 0.0 0.0 0.0180253028082411 0.0 44-45 0.0 0.0 0.0 0.024342581829820926 0.0 46-47 0.0 0.0 0.0 0.0397146274489985 0.0 48-49 0.0 0.0 0.0 0.0791765637371338 0.0 50-51 0.0 0.0 0.0 0.10301376324522835 0.0 52-53 0.0 0.0 0.0 0.12899883762066003 0.0 54-55 0.0 0.0 0.0 0.16248041643503308 0.0 56-57 0.0 0.0 0.0 0.18560165765401526 0.0 58-59 0.0 0.0 0.0 0.21234480551203652 0.0 60-61 0.0 0.0 0.0 0.25719748656525326 0.0 62-63 0.0 0.0 0.0 0.288489075318812 0.0 64-65 0.0 0.0 0.0 0.32424487458095386 0.0 66-67 0.0 0.0 0.0 0.39508263000960225 0.0 68-69 0.0 0.0 0.0 0.4740907329728273 0.0 70-71 0.0 0.0 0.0 0.5362106433516955 0.0 72-73 0.0 0.0 0.0 0.611933761223699 0.0 74-75 0.0 0.0 0.0 0.684161318037095 0.0 76-77 0.0 0.0 0.0 0.7378160745270463 0.0 78-79 0.0 0.0 0.0 0.8067586462492209 0.0 80-81 0.0 0.0 0.0 0.9216488940550193 0.0 82-83 0.0 0.0 0.0 1.0278212968110374 0.0 84-85 0.0 0.0 0.0 1.1583362813968767 0.0 86-87 0.0 0.0 0.0 1.4175131820555582 0.0 88-89 0.0 0.0 0.0 1.7263017806303802 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 8290 0.0 33.8867 1 GGTATCA 5355 0.0 31.156162 1 ATCAACG 9950 0.0 27.99556 3 TATCAAC 10020 0.0 27.847422 2 TCAACGC 10015 0.0 27.81386 4 CAACGCA 10075 0.0 27.644724 5 AACGCAG 10260 0.0 27.097647 6 GTACATG 6310 0.0 25.38615 1 ACGCAGA 11200 0.0 24.688795 7 CGCAGAG 11220 0.0 24.475405 8 TACATGG 6540 0.0 24.203663 2 ACATGGG 6310 0.0 23.805227 3 GAGTACT 5495 0.0 23.561995 12-13 GCAGAGT 11470 0.0 23.28017 9 CATGGGG 2420 0.0 22.589005 4 AGTACTT 5770 0.0 21.574398 12-13 GTACTTT 6005 0.0 21.283516 14-15 TACACTG 945 0.0 20.118141 5 CTCGCGC 145 2.4501787E-9 19.650236 60-61 AGAGTAC 10310 0.0 19.562857 10-11 >>END_MODULE