FastQCFastQC Report
Thu 26 May 2016
SRR936968_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936968_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1187220
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77780.6551439497313051No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59740.5031923316655718No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43590.3671602567342194No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15020.1265140412055053No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA12370.10419298866258993No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59250.032.9624631
GTATCAA90350.031.9772851
ATCAACG109550.025.9704423
TCAACGC110050.025.809294
TATCAAC111550.025.5473942
CAACGCA112150.025.3260125
AACGCAG115300.024.7175356
GTACATG64400.023.6118641
GAGTACT65050.022.74781412-13
TACATGG66650.022.661672
ACGCAGA125100.022.5913857
CGCAGAG124900.022.4402488
ACATGGG65650.021.849283
AGTACTT68200.021.766812-13
GTACTTT68950.021.28814-15
GCAGAGT129450.021.2111329
CATGGGG27900.019.407324
AGAGTAC114850.019.1508510-11
TACTTTT76600.019.00694714-15
CATGGGA40100.018.9513664
Produced by FastQC (version 0.11.5)