##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936968_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1187220 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.11961557251394 31.0 31.0 34.0 31.0 34.0 2 32.24643368541635 33.0 31.0 34.0 30.0 34.0 3 32.367204898839304 34.0 31.0 34.0 31.0 34.0 4 36.04790855949192 37.0 35.0 37.0 35.0 37.0 5 35.88675898317077 37.0 35.0 37.0 35.0 37.0 6 35.98262242886744 37.0 35.0 37.0 35.0 37.0 7 35.938792304711846 37.0 35.0 37.0 35.0 37.0 8 35.88975000421152 37.0 35.0 37.0 35.0 37.0 9 37.57937197823487 39.0 37.0 39.0 35.0 39.0 10-11 37.48905636697495 39.0 37.0 39.0 35.0 39.0 12-13 37.38379154663836 39.0 37.0 39.0 34.0 39.0 14-15 38.5483697208605 40.0 38.0 41.0 34.0 41.0 16-17 38.54572109634272 40.0 38.0 41.0 34.0 41.0 18-19 38.68906689577332 40.0 38.0 41.0 34.0 41.0 20-21 38.697686191270364 40.0 38.0 41.0 34.0 41.0 22-23 38.60316453563787 40.0 38.0 41.0 34.0 41.0 24-25 38.50277918161756 40.0 38.0 41.0 34.0 41.0 26-27 38.29339844342245 40.0 38.0 41.0 34.0 41.0 28-29 38.13298756759488 40.0 38.0 41.0 33.5 41.0 30-31 38.09739054261215 40.0 37.0 41.0 34.0 41.0 32-33 37.94817220060309 40.0 37.0 41.0 33.0 41.0 34-35 37.814359175216055 40.0 37.0 41.0 33.0 41.0 36-37 37.650286383315645 40.0 37.0 41.0 33.0 41.0 38-39 37.47519162413032 40.0 36.5 41.0 33.0 41.0 40-41 37.128335944475324 39.5 36.0 41.0 31.5 41.0 42-43 36.91715899327842 39.0 36.0 41.0 31.0 41.0 44-45 36.65743164704099 39.0 36.0 41.0 31.0 41.0 46-47 36.23037474099156 39.0 35.0 40.5 30.0 41.0 48-49 35.822248193258204 39.0 35.0 40.0 29.0 41.0 50-51 35.5292245750577 39.0 35.0 40.0 28.0 41.0 52-53 35.25195414497734 38.5 35.0 40.0 27.0 41.0 54-55 34.85641035359916 38.0 34.0 40.0 26.0 41.0 56-57 34.671432421960546 38.0 34.0 40.0 26.0 41.0 58-59 34.35110594498071 38.0 34.0 40.0 25.5 41.0 60-61 33.971745759000015 37.0 33.0 40.0 24.5 41.0 62-63 33.59146830410539 37.0 33.0 39.0 23.5 40.5 64-65 33.26458575495696 36.0 33.0 39.0 23.0 40.0 66-67 32.93227287276158 36.0 32.0 39.0 22.0 40.0 68-69 32.480778625697006 35.0 31.5 38.5 21.5 40.0 70-71 32.03891191186132 35.0 31.0 38.0 20.0 40.0 72-73 31.617828624854702 35.0 31.0 37.0 18.5 39.0 74-75 31.130129630565524 34.5 30.5 36.5 17.5 39.0 76-77 29.69890837418507 33.0 28.5 35.0 14.0 37.5 78-79 30.379314701571737 34.0 30.0 36.0 14.0 38.0 80-81 30.24730504876939 34.0 30.0 35.0 11.5 37.0 82-83 29.902358871986657 34.0 30.0 35.0 7.5 37.0 84-85 29.453215916173917 34.0 29.0 35.0 6.0 36.0 86-87 29.0611925338185 34.0 29.0 35.0 2.0 36.0 88-89 28.667209531510586 34.0 29.0 35.0 2.0 35.5 90-91 28.198171779451155 33.0 28.5 35.0 2.0 35.0 92-93 27.69250349556106 33.0 27.0 35.0 2.0 35.0 94-95 27.122495830595845 33.0 26.0 34.0 2.0 35.0 96-97 26.320035882144843 32.0 25.0 34.0 2.0 35.0 98-99 25.434411061134416 32.0 22.0 34.0 2.0 35.0 100-101 22.261507134313774 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.134344907955942 1101 2 0.24858204783441806 1101 3 0.13254413341887528 1101 4 0.08665567184254996 1101 5 0.03600520644814509 1101 6 0.08959451482387237 1101 7 0.04149036543268636 1101 8 0.11260496469593306 1101 9 0.0014425198151641894 1101 10-11 0.051108930400651786 1101 12-13 0.17995283572782483 1101 14-15 0.3460749719837466 1101 16-17 0.3207856042555193 1101 18-19 0.1512466729787718 1101 20-21 0.05500511612837755 1101 22-23 0.18218492244183437 1101 24-25 0.15890356009191464 1101 26-27 0.12471759789110592 1101 28-29 0.2568699425089207 1101 30-31 0.07600244968560332 1101 32-33 0.04653240898795019 1101 34-35 0.10411928010113058 1101 36-37 0.13965511474751224 1101 38-39 0.24731531654099825 1101 40-41 0.25812171710878573 1101 42-43 0.1654670997804999 1101 44-45 -0.004413969130609985 1101 46-47 -0.13183037594777858 1101 48-49 0.08967863391588082 1101 50-51 0.26861026384770526 1101 52-53 0.25936072958847944 1101 54-55 0.1583045066771831 1101 56-57 0.19161028453494566 1101 58-59 0.4017608560157342 1101 60-61 0.4337363788106643 1101 62-63 0.21525758108640503 1101 64-65 0.12842821766391666 1101 66-67 0.42473828464427044 1101 68-69 0.21404810780645533 1101 70-71 -0.08919019189452726 1101 72-73 0.008784955474105516 1101 74-75 0.1617518171093444 1101 76-77 0.31615105639057717 1101 78-79 0.18327441393469357 1101 80-81 0.0012213000061116475 1101 82-83 0.31770644808104365 1101 84-85 0.20717501975579822 1101 86-87 -0.10488363043987192 1101 88-89 0.07142018425648544 1101 90-91 0.2322420936045333 1101 92-93 -0.016792053853333044 1101 94-95 -0.16185441577661663 1101 96-97 0.07146870768515257 1101 98-99 0.10935463203565376 1101 100-101 0.40689774722916283 1102 1 -0.07964538693020984 1102 2 0.0031098567183320824 1102 3 -0.009889610515429581 1102 4 0.08152689244725764 1102 5 -0.07221427843427364 1102 6 0.02836271191234374 1102 7 0.011314926836192285 1102 8 0.07964490497215593 1102 9 0.1328124339622363 1102 10-11 0.1530238240176729 1102 12-13 0.14295768828474564 1102 14-15 0.20193910038987184 1102 16-17 0.16391736983969452 1102 18-19 0.19698762109374002 1102 20-21 0.05125744908843188 1102 22-23 -0.25632571585603614 1102 24-25 -0.17381013904955012 1102 26-27 0.04365003536778289 1102 28-29 -0.2606813826123968 1102 30-31 -0.03356828566341363 1102 32-33 -0.09446423154956562 1102 34-35 0.11218199006753338 1102 36-37 0.2343471640196313 1102 38-39 0.28335488354360905 1102 40-41 0.43775964170752246 1102 42-43 0.3417492946293237 1102 44-45 0.46133817719265124 1102 46-47 0.5674716024023567 1102 48-49 0.41646101540150937 1102 50-51 0.4453179906114144 1102 52-53 0.5340490461245011 1102 54-55 0.7123918527018205 1102 56-57 0.44011345736062424 1102 58-59 0.3051800422792681 1102 60-61 0.47538624816489516 1102 62-63 0.30682159751044935 1102 64-65 0.36466188694350166 1102 66-67 0.22737359632773746 1102 68-69 0.44230865278593257 1102 70-71 0.01578181258475908 1102 72-73 0.01749342878503768 1102 74-75 0.3252941836640275 1102 76-77 0.3635792012207375 1102 78-79 -0.038569557696511936 1102 80-81 0.43238964640402244 1102 82-83 0.558374607842147 1102 84-85 0.46830603879275046 1102 86-87 0.4320219313369087 1102 88-89 0.21255866876562735 1102 90-91 0.13922604283932216 1102 92-93 0.15890036869239665 1102 94-95 0.3441515566011333 1102 96-97 0.47372328028686894 1102 98-99 0.49629752117339265 1102 100-101 0.6266775157995248 1103 1 0.05608543390699339 1103 2 0.16732005709874187 1103 3 0.10440684633422137 1103 4 0.12122108746059723 1103 5 0.01168598844471802 1103 6 -0.02599508287098473 1103 7 0.08560460112587265 1103 8 0.10121786909831343 1103 9 0.027168164488180935 1103 10-11 0.12613318542490504 1103 12-13 0.2302617681420145 1103 14-15 0.24744565440706623 1103 16-17 0.09190206484565522 1103 18-19 0.19012838870258975 1103 20-21 0.2138000243969671 1103 22-23 0.22449954475645484 1103 24-25 0.20065794605683607 1103 26-27 0.17715440717002195 1103 28-29 0.10523920245712048 1103 30-31 0.23615053299158006 1103 32-33 0.05690407620172522 1103 34-35 0.17969647673096034 1103 36-37 0.37477711934320723 1103 38-39 0.19583995453931635 1103 40-41 0.39336262603390537 1103 42-43 0.30776129681154174 1103 44-45 0.326180187779336 1103 46-47 0.41250853786482367 1103 48-49 0.46860047073245426 1103 50-51 0.24761963759391392 1103 52-53 0.21218634872988673 1103 54-55 0.4143573206247382 1103 56-57 0.2188678254766998 1103 58-59 0.12609539298184558 1103 60-61 -0.08128204041828724 1103 62-63 0.14198917097589003 1103 64-65 0.150252633172542 1103 66-67 0.129201938581609 1103 68-69 0.116947039126444 1103 70-71 0.10442544803690268 1103 72-73 0.493237667295233 1103 74-75 0.1640292362288669 1103 76-77 0.2193746038246509 1103 78-79 0.3075710182313003 1103 80-81 0.5015422687481355 1103 82-83 0.5489534845912338 1103 84-85 0.16806643748931904 1103 86-87 -0.03845550190647984 1103 88-89 0.14314137913557445 1103 90-91 -0.14053465075116023 1103 92-93 -0.346709987981793 1103 94-95 -0.18684547971504983 1103 96-97 -0.09423797065310424 1103 98-99 -0.16334624698101408 1103 100-101 0.359311213326837 1104 1 0.036431244128323215 1104 2 0.15063053003577664 1104 3 -0.07356637105808517 1104 4 -0.07065815213648108 1104 5 -0.25152918970821503 1104 6 -0.020314490053465306 1104 7 -0.06309724467141109 1104 8 -0.09371373662167315 1104 9 -0.07914130489183435 1104 10-11 0.025327464287236978 1104 12-13 0.030940678779430186 1104 14-15 0.2251373582398415 1104 16-17 0.090327716798285 1104 18-19 0.13963351144422376 1104 20-21 0.09615165467391051 1104 22-23 0.16269667901168106 1104 24-25 0.09104522129887727 1104 26-27 0.10952214036763053 1104 28-29 0.11796697970772385 1104 30-31 0.03671657940824247 1104 32-33 -0.04293690745668499 1104 34-35 0.06607239941393317 1104 36-37 0.05153471000120646 1104 38-39 -0.018412280459799035 1104 40-41 0.07872665268423873 1104 42-43 -0.08334413993704715 1104 44-45 0.05769202198826662 1104 46-47 -0.027419660362134834 1104 48-49 -0.0988887388729296 1104 50-51 -0.006922967571100003 1104 52-53 0.009129237996425843 1104 54-55 -0.013734512031412294 1104 56-57 0.12041715648661011 1104 58-59 -0.2685361520646339 1104 60-61 -0.2188340514142766 1104 62-63 -0.26423575807061894 1104 64-65 -0.14192120360710447 1104 66-67 -0.2977389665750607 1104 68-69 -0.24749152966897014 1104 70-71 -0.24943551474522252 1104 72-73 -0.16001900463363228 1104 74-75 -0.2471919115068033 1104 76-77 0.011224944430178851 1104 78-79 0.046635409785316995 1104 80-81 -0.13614129735866598 1104 82-83 0.037084769403019635 1104 84-85 -0.10691393707611851 1104 86-87 -0.18977407231218635 1104 88-89 0.06864883680070832 1104 90-91 0.14995556816229794 1104 92-93 -0.07724630150565659 1104 94-95 0.08872940893037651 1104 96-97 0.0880312759314954 1104 98-99 -0.09806256240272404 1104 100-101 0.7731181738142965 1105 1 -0.02329464547308646 1105 2 0.13353883727101135 1105 3 0.1373837165203966 1105 4 0.05544169609981253 1105 5 0.033442986945907194 1105 6 -0.012403185558156338 1105 7 0.05754466608919273 1105 8 0.139955582254629 1105 9 0.15598278167488644 1105 10-11 -0.004860061847409725 1105 12-13 0.10111896458060698 1105 14-15 0.251365576931633 1105 16-17 -0.01858978983224091 1105 18-19 0.1042402563028304 1105 20-21 0.18593275234365336 1105 22-23 0.07787202603591226 1105 24-25 0.0019264449997464794 1105 26-27 0.06395501490086275 1105 28-29 0.07970366529368533 1105 30-31 0.13435488468430634 1105 32-33 0.04701013607522242 1105 34-35 0.11232743261772526 1105 36-37 0.139236732640029 1105 38-39 -0.014188336884316755 1105 40-41 -0.057159615186009205 1105 42-43 0.12193162260977175 1105 44-45 0.14497589029597435 1105 46-47 0.14794856400252598 1105 48-49 0.16325528166633774 1105 50-51 0.27760473734645785 1105 52-53 0.287214295850859 1105 54-55 0.49128918825427803 1105 56-57 0.19200196140153025 1105 58-59 0.3421614346292827 1105 60-61 0.4315450654320756 1105 62-63 0.29294757774825086 1105 64-65 0.4197148910068975 1105 66-67 0.37786465236060707 1105 68-69 0.30604172822113185 1105 70-71 0.13752172531922824 1105 72-73 0.1739093944785921 1105 74-75 0.19468902270260813 1105 76-77 0.16678493495590985 1105 78-79 0.3540848438148885 1105 80-81 0.09859899371183545 1105 82-83 0.024497739205255442 1105 84-85 -0.34393287683557716 1105 86-87 -0.043773879317878794 1105 88-89 0.16127367633763967 1105 90-91 0.2034464257880586 1105 92-93 0.2018059209992309 1105 94-95 0.43059616098240383 1105 96-97 0.5861773609250172 1105 98-99 0.7251656182750388 1105 100-101 1.1130681415023709 1106 1 -0.011593013771459937 1106 2 0.05501929479798662 1106 3 0.08157247349984686 1106 4 0.03183785807736683 1106 5 -0.0557221889634576 1106 6 0.0730762962003908 1106 7 0.08721236273363075 1106 8 0.12803006591050803 1106 9 0.12343982075983462 1106 10-11 0.0323909816827026 1106 12-13 0.1644197723000218 1106 14-15 0.27869305565446467 1106 16-17 0.10088681883041772 1106 18-19 0.16582831940252873 1106 20-21 0.1298499204468655 1106 22-23 0.060518255775164675 1106 24-25 0.13399127939015898 1106 26-27 0.2635300135456262 1106 28-29 0.28515604237396275 1106 30-31 0.15654900839005137 1106 32-33 0.12944738874502804 1106 34-35 -0.0030062259717382744 1106 36-37 0.11949991906600133 1106 38-39 -0.005532186832816421 1106 40-41 0.19933629200757252 1106 42-43 0.11365873270897708 1106 44-45 0.053345814944968595 1106 46-47 0.015193915550206327 1106 48-49 0.1255963127282982 1106 50-51 0.18462240959668463 1106 52-53 0.09827435981789989 1106 54-55 0.260376031643446 1106 56-57 0.09346588507475673 1106 58-59 0.06625478314123967 1106 60-61 -0.027505186641427315 1106 62-63 -0.03291380892709128 1106 64-65 0.01938638230629408 1106 66-67 0.03509590947557939 1106 68-69 0.009992777172179501 1106 70-71 0.24190292551437764 1106 72-73 0.10223974629731458 1106 74-75 -0.0064870492874185 1106 76-77 -0.09145353200348438 1106 78-79 -0.2421102014499219 1106 80-81 -0.1810391588332898 1106 82-83 -0.05804928741153503 1106 84-85 0.17137205079493256 1106 86-87 0.2033012548502775 1106 88-89 0.34632571731991035 1106 90-91 0.06690471168820267 1106 92-93 0.2935048422330375 1106 94-95 0.4689687960992259 1106 96-97 0.3460773446622092 1106 98-99 0.6190307453959782 1106 100-101 0.8931123221279478 1107 1 0.3598343298346549 1107 2 0.35308240487226783 1107 3 0.27077070543550974 1107 4 0.21377290626939782 1107 5 0.08849845073203966 1107 6 0.13556754796538684 1107 7 0.2599448816114034 1107 8 0.22558854095501601 1107 9 0.27629389406083504 1107 10-11 0.2357005771152032 1107 12-13 0.3294839260233928 1107 14-15 0.482476590555315 1107 16-17 0.45863161593275237 1107 18-19 0.34162149270966324 1107 20-21 0.31587852871010824 1107 22-23 0.18013118474563328 1107 24-25 0.4326345355753176 1107 26-27 0.3456271343019708 1107 28-29 0.3185061559475386 1107 30-31 0.3473461721201758 1107 32-33 0.42423896588298504 1107 34-35 0.5502947747035591 1107 36-37 0.4467695488775334 1107 38-39 0.4604473085321459 1107 40-41 0.5635108883609448 1107 42-43 0.5432781077473408 1107 44-45 0.5685155613996287 1107 46-47 0.6432542630239553 1107 48-49 0.37309247578312466 1107 50-51 0.23366255939102132 1107 52-53 0.4671505953142443 1107 54-55 0.7738811578022009 1107 56-57 0.604698311865846 1107 58-59 0.6392825933198836 1107 60-61 0.5631817800231858 1107 62-63 0.571288720387173 1107 64-65 0.7115295464047691 1107 66-67 0.7815680686013664 1107 68-69 0.5523959326758998 1107 70-71 0.4903687613545209 1107 72-73 0.6691456337059947 1107 74-75 0.6050768895670267 1107 76-77 0.2655404516664781 1107 78-79 0.4873925304550468 1107 80-81 0.3286726098449577 1107 82-83 0.4440880445302646 1107 84-85 0.6067756974276293 1107 86-87 0.7243175848889543 1107 88-89 0.7994545158956363 1107 90-91 0.7971395327330804 1107 92-93 0.8421824774469862 1107 94-95 1.0490031751280178 1107 96-97 1.052834972509224 1107 98-99 0.940394242234337 1107 100-101 1.1494616990080502 1108 1 -0.03713010474284317 1108 2 0.027199638581890895 1108 3 -0.05581830943658872 1108 4 0.010334802854934821 1108 5 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-0.7230459512569354 2316 64-65 -0.643467596728108 2316 66-67 -0.9261480001656395 2316 68-69 -1.0326862424646102 2316 70-71 -0.8505125476880409 2316 72-73 -0.9678089765729041 2316 74-75 -0.7787290395428421 2316 76-77 -0.9273792665376668 2316 78-79 -0.6734050486372851 2316 80-81 -0.8756921929393009 2316 82-83 -0.7818120217980713 2316 84-85 -0.6541439686942141 2316 86-87 -0.6217991437913888 2316 88-89 -1.4693977434803251 2316 90-91 -1.30994971028138 2316 92-93 -1.0546244948406382 2316 94-95 -1.1482968173660026 2316 96-97 -0.776929447010609 2316 98-99 -1.449169461307111 2316 100-101 -2.263468956478281 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 5.0 4 6.0 5 25.0 6 70.0 7 138.0 8 352.0 9 707.0 10 1227.0 11 1999.0 12 3060.0 13 4372.0 14 5495.0 15 7494.0 16 10051.0 17 14042.0 18 12504.0 19 7463.0 20 6352.0 21 6697.0 22 6803.0 23 7480.0 24 8727.0 25 10396.0 26 12885.0 27 15926.0 28 19760.0 29 24685.0 30 30783.0 31 38778.0 32 48830.0 33 64156.0 34 87759.0 35 123424.0 36 185229.0 37 259280.0 38 152932.0 39 7328.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.348279127448706 19.869397012189165 15.18944257411395 21.592881286248183 2 15.55922438046463 21.52681143550262 39.85149682440742 23.06246735962533 3 19.160472197537935 26.285466406674445 30.37495314664993 24.179108249137688 4 12.887249204022844 17.772780108151817 37.61122622597328 31.72874446185206 5 13.356345540247776 38.329728199892855 33.800737199249326 14.513189060610044 6 30.43285995855865 37.456326544364146 17.56220414076582 14.548609356311381 7 27.505877180200116 34.042918787519405 20.94466143146294 17.50654260081754 8 26.434527720220345 34.665858054951904 19.81839928572632 19.08121493910143 9 26.943975795535618 16.6742614462875 20.724061862217503 35.65770089595938 10-11 24.634440120617914 27.516761847003924 27.09784201748623 20.750956014891933 12-13 26.111352120862414 24.956807776706103 27.959751839512776 20.97208826291871 14-15 22.95780822979372 25.765159896949637 27.0912362969087 24.185795576347946 16-17 21.363732079985176 29.276460976061724 27.527416990953657 21.832389952999446 18-19 21.332424761721057 27.223371741260934 30.757451389242775 20.686752107775234 20-21 22.34959867992692 26.31411371395216 30.850896263246426 20.485391342874497 22-23 22.047974326091488 26.32161034705683 30.68651172193338 20.943903604918304 24-25 21.878906210516742 26.53296125713015 30.827392243339858 20.760740289013253 26-27 21.73331485874733 26.677526803241026 30.753068206178064 20.83609013183358 28-29 21.3293840479305 26.650558995433865 31.035891440416634 20.984165516218997 30-31 21.447541315004802 26.614864978689713 30.912509897070468 21.02508380923502 32-33 21.36590082769234 26.760873016842336 30.76401818876338 21.109207966701945 34-35 21.840770876501406 26.664560906992808 30.580726402857096 20.91394181364869 36-37 21.91987190016164 26.395995953500634 30.506764240872847 21.17736790546488 38-39 21.631713197349512 26.757178405899406 30.641343372347112 20.96976502440397 40-41 21.73455266165829 26.55703156389218 30.62168392981534 21.086731844634187 42-43 21.629394720439347 26.798571452637255 30.254165192634897 21.317868634288505 44-45 21.74457066952828 27.088448621935985 29.894609963367863 21.27237074516787 46-47 21.964284510990417 27.23211277476045 29.28995229174809 21.513650422501044 48-49 22.22245619591347 27.308463988922 28.934702809425062 21.53437700573947 50-51 22.150320986489632 27.518753166590788 28.73266034899779 21.59826549792179 52-53 22.302845148389423 27.638319628980117 28.608827052241637 21.450008170388827 54-55 21.956292852209362 27.629251528781523 28.72138272603224 21.69307289297687 56-57 22.109877225374323 27.987451314672306 28.37992412501179 21.522747334941577 58-59 22.1501770735547 27.63665660533423 28.525541001930144 21.687625319180924 60-61 22.213607165780267 27.520135647128786 28.78904782521534 21.47720936187561 62-63 22.211604509528357 27.578387989752528 28.82630381323757 21.38370368748155 64-65 22.139184994169145 27.547119590875646 28.700782543398706 21.6129128715565 66-67 22.045165194810227 27.821771795358817 28.453078811458198 21.679984198372754 68-69 21.985824862209558 27.760746854309588 28.63665901714047 21.616769266340384 70-71 22.02567939377527 27.78355927499323 28.473534523821055 21.71722680741044 72-73 22.23038322801003 27.64889331567299 28.243266292823343 21.87745716349363 74-75 22.11769413003422 27.99861016056857 28.09901553040274 21.784680178994474 76-77 22.193747659973575 27.90958201074022 28.163578849572467 21.733091479713735 78-79 22.116718938662984 27.88544999471454 28.102216986833522 21.895614079788952 80-81 22.010435474041664 28.334304104487828 27.9499545359019 21.705305885568613 82-83 22.183083169084078 28.203660652617042 27.839574805006656 21.773681373292227 84-85 22.08898109494978 28.035471817627418 27.714589671798084 22.160957415624715 86-87 22.123840881658534 27.854596084793258 27.999262139945774 22.022300893602434 88-89 22.328818470505986 27.67838340956376 27.850259857310117 22.142538262620135 90-91 22.095711315353576 27.81447693764896 28.096946990221994 21.99286475677547 92-93 22.095399336264553 27.76355688078031 28.00993076262192 22.131113020333217 94-95 21.93334007176429 27.743804855039507 27.925026532571888 22.397828540624314 96-97 22.070284450587653 27.616062249703827 27.919502602090517 22.394150697618002 98-99 21.8763147886799 27.73296002284337 27.668944686116383 22.721780502360357 100-101 21.835349076585047 28.02303578219781 27.609035235531778 22.532579905685367 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 811.0 1 570.5 2 371.5 3 441.5 4 394.5 5 370.5 6 647.5 7 1398.5 8 2289.5 9 2651.5 10 2317.5 11 1911.0 12 1914.0 13 2043.0 14 2190.5 15 2292.0 16 2332.5 17 2458.0 18 2576.0 19 2741.0 20 3005.5 21 3353.5 22 3801.5 23 4418.0 24 5314.0 25 6367.5 26 7897.0 27 9472.0 28 10727.0 29 12421.0 30 14752.5 31 17067.0 32 19456.0 33 22273.0 34 25681.0 35 28309.5 36 31310.0 37 34461.5 38 35523.0 39 36132.5 40 36448.0 41 37280.0 42 38530.0 43 39955.5 44 41412.5 45 42267.0 46 42074.0 47 42506.0 48 47541.5 49 47524.0 50 42525.5 51 40011.5 52 37932.0 53 36776.0 54 35265.5 55 32860.5 56 29707.0 57 26128.0 58 23421.5 59 21310.0 60 18690.0 61 15446.5 62 12145.0 63 9432.0 64 7147.5 65 5370.5 66 3882.0 67 2974.5 68 2128.0 69 1275.0 70 896.5 71 663.0 72 481.0 73 364.5 74 271.5 75 191.5 76 143.0 77 82.0 78 37.0 79 24.0 80 12.0 81 9.0 82 8.0 83 3.0 84 2.0 85 1.5 86 1.5 87 0.5 88 0.5 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022236822155960984 2 0.003369215478175907 3 4.2115193477198836E-4 4 0.0 5 0.002021529286905544 6 0.0 7 8.423038695439767E-5 8 0.0 9 8.423038695439767E-5 10-11 0.0 12-13 0.007159582891123803 14-15 0.002821717962972322 16-17 0.0 18-19 7.159582891123802E-4 20-21 0.0010949950304071697 22-23 0.0018951837064739474 24-25 6.738430956351813E-4 26-27 2.948063543403918E-4 28-29 8.423038695439767E-5 30-31 0.0 32-33 2.948063543403918E-4 34-35 0.0 36-37 0.001768838126042351 38-39 0.009939185660618924 40-41 7.58073482589579E-4 42-43 0.0 44-45 9.265342564983743E-4 46-47 3.3692154781759067E-4 48-49 1.6846077390879533E-4 50-51 0.0017267229325651522 52-53 5.05382321726386E-4 54-55 0.0 56-57 3.3692154781759067E-4 58-59 1.263455804315965E-4 60-61 0.0018530685129967486 62-63 4.2115193477198834E-5 64-65 1.263455804315965E-4 66-67 4.2115193477198834E-5 68-69 4.2115193477198834E-5 70-71 2.948063543403918E-4 72-73 2.1057596738599418E-4 74-75 0.002737487576017924 76-77 4.2115193477198834E-5 78-79 4.2115193477198834E-5 80-81 0.0016424925456107544 82-83 0.0 84-85 0.002021529286905544 86-87 2.52691160863193E-4 88-89 0.00252691160863193 90-91 0.001221340610838766 92-93 0.0 94-95 0.0 96-97 1.263455804315965E-4 98-99 3.790367412947895E-4 100-101 0.0019794140934283454 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1187220.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.70603956953387 #Duplication Level Percentage of deduplicated Percentage of total 1 79.5768961987521 46.716444170646305 2 11.983407435965049 14.069967822272215 3 3.693640237891707 6.5051696988387935 4 1.51396507569423 3.555155745623913 5 0.7953501293601303 2.3345928082924847 6 0.5013295499239625 1.7658643437127652 7 0.3311607237782448 1.3608794187800783 8 0.24496802585983632 1.150488209551852 9 0.1713997615557688 0.9055981065691473 >10 1.0589011786451619 11.721796861163938 >50 0.08712202855143762 3.504504718884549 >100 0.03970194387650901 4.000347403385323 >500 0.0011507787445381383 0.45348080961605897 >1k 7.192367153363365E-4 0.7943936640704407 >5k 2.8769468613453457E-4 1.1613162185921866 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7778 0.6551439497313051 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5974 0.5031923316655718 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4359 0.3671602567342194 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1502 0.1265140412055053 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1237 0.10419298866258993 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 4.2115193477198834E-5 18-19 0.0 0.0 0.0 0.0 8.423038695439767E-5 20-21 0.0 0.0 0.0 4.2115193477198834E-5 8.423038695439767E-5 22-23 0.0 0.0 0.0 2.948063543403919E-4 8.423038695439767E-5 24-25 0.0 0.0 0.0 7.159582891123801E-4 8.423038695439767E-5 26-27 0.0 0.0 0.0 8.844190630211755E-4 8.423038695439767E-5 28-29 0.0 0.0 0.0 0.002021529286905544 8.423038695439767E-5 30-31 0.0 0.0 0.0 0.0038745977999022924 8.423038695439767E-5 32-33 0.0 0.0 0.0 0.006275163828102627 8.423038695439767E-5 34-35 0.0 0.0 0.0 0.008001886760667778 8.423038695439767E-5 36-37 0.0 0.0 0.0 0.009939185660618924 8.423038695439767E-5 38-39 0.0 0.0 0.0 0.011455332625798082 8.423038695439767E-5 40-41 0.0 0.0 0.0 0.0144455113626792 8.423038695439767E-5 42-43 0.0 0.0 0.0 0.018109533195195496 8.423038695439767E-5 44-45 0.0 0.0 0.0 0.024637388184161318 8.423038695439767E-5 46-47 0.0 0.0 0.0 0.04022000977072489 8.423038695439767E-5 48-49 0.0 0.0 0.0 0.08018732838058658 8.423038695439767E-5 50-51 0.0 0.0 0.0 0.10410875827563551 8.423038695439767E-5 52-53 0.0 0.0 0.0 0.1299253718771584 8.423038695439767E-5 54-55 0.0 0.0 0.0 0.16290156836980507 8.423038695439767E-5 56-57 0.0 0.0 0.0 0.18635973113660484 8.423038695439767E-5 58-59 0.0 0.0 0.0 0.2133134549620121 8.423038695439767E-5 60-61 0.0 0.0 0.0 0.2589663246912956 8.423038695439767E-5 62-63 0.0 0.0 0.0 0.2907211805731036 8.423038695439767E-5 64-65 0.0 0.0 0.0 0.32660332541567694 8.423038695439767E-5 66-67 0.0 0.0 0.0 0.39765165681171144 8.423038695439767E-5 68-69 0.0 0.0 0.0 0.4764070686140732 8.423038695439767E-5 70-71 0.0 0.0 0.0 0.5380215966712152 8.423038695439767E-5 72-73 0.0 0.0 0.0 0.6137447145432187 8.423038695439767E-5 74-75 0.0 0.0 0.0 0.6859301561631375 8.423038695439767E-5 76-77 0.0 0.0 0.0 0.7400060645878608 8.423038695439767E-5 78-79 0.0 0.0 0.0 0.8093697882448072 8.423038695439767E-5 80-81 0.0 0.0 0.0 0.924133690470174 8.423038695439767E-5 82-83 0.0 0.0 0.0 1.0304324388066237 8.423038695439767E-5 84-85 0.0 0.0 0.0 1.1597260827816243 8.423038695439767E-5 86-87 0.0 0.0 0.0 1.4182712555381478 8.423038695439767E-5 88-89 0.0 0.0 0.0 1.7272283148868786 8.423038695439767E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5925 0.0 32.962463 1 GTATCAA 9035 0.0 31.977285 1 ATCAACG 10955 0.0 25.970442 3 TCAACGC 11005 0.0 25.80929 4 TATCAAC 11155 0.0 25.547394 2 CAACGCA 11215 0.0 25.326012 5 AACGCAG 11530 0.0 24.717535 6 GTACATG 6440 0.0 23.611864 1 GAGTACT 6505 0.0 22.747814 12-13 TACATGG 6665 0.0 22.66167 2 ACGCAGA 12510 0.0 22.591385 7 CGCAGAG 12490 0.0 22.440248 8 ACATGGG 6565 0.0 21.84928 3 AGTACTT 6820 0.0 21.7668 12-13 GTACTTT 6895 0.0 21.288 14-15 GCAGAGT 12945 0.0 21.211132 9 CATGGGG 2790 0.0 19.40732 4 AGAGTAC 11485 0.0 19.15085 10-11 TACTTTT 7660 0.0 19.006947 14-15 CATGGGA 4010 0.0 18.951366 4 >>END_MODULE