FastQCFastQC Report
Thu 26 May 2016
SRR936967_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936967_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1244196
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182521.466971441798559No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126651.0179264360277642No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90370.7263325070969525No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26720.21475716044738932No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23090.18558169291655013No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19150.153914656533215No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17650.14185867821468642No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12880.1035206671617655No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67700.050.68821
GTATCAA124350.043.6494331
TATCAAC161450.033.502722
ATCAACG162250.033.33353
TCAACGC164050.032.9690864
CAACGCA166100.032.501035
AACGCAG170050.031.8845256
GTCGTAT650.00582686929.2485921
ACGCAGA189550.028.378777
CGCAGAG189700.028.2834598
AGGATCG856.3906895E-427.9537125
GCAGAGT194550.027.042069
GTACATG108200.026.4879531
TACATGG110000.025.6234652
GAGTACT115600.024.91018512-13
ACATGGG111500.024.0822433
AGTACTT117800.024.0214212-13
GTACTTT123450.023.20977614-15
ATGGGAT29650.022.2781185
CATGGGA81400.022.2454624
Produced by FastQC (version 0.11.5)