##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936967_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1244196 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.867397098206393 33.0 31.0 34.0 30.0 34.0 2 31.928865709261242 33.0 31.0 34.0 30.0 34.0 3 31.90410353352687 33.0 31.0 34.0 30.0 34.0 4 35.53927435870233 37.0 35.0 37.0 33.0 37.0 5 35.583539892428526 37.0 35.0 37.0 33.0 37.0 6 35.67810136023585 37.0 35.0 37.0 33.0 37.0 7 35.62466685313247 37.0 35.0 37.0 33.0 37.0 8 35.5956063192616 37.0 35.0 37.0 33.0 37.0 9 37.20182672183482 39.0 37.0 39.0 34.0 39.0 10-11 37.08122393899353 39.0 37.0 39.0 33.0 39.0 12-13 36.977945596995966 39.0 37.0 39.0 33.0 39.0 14-15 38.02855257531772 40.0 38.0 41.0 33.0 41.0 16-17 37.99738787136432 40.0 38.0 41.0 33.0 41.0 18-19 38.070752919957954 40.0 38.0 41.0 33.0 41.0 20-21 38.079714932374 40.0 38.0 41.0 33.0 41.0 22-23 37.95153094850008 40.0 38.0 41.0 33.0 41.0 24-25 37.7823039939045 40.0 37.0 41.0 33.0 41.0 26-27 37.69589397490428 40.0 37.0 41.0 33.0 41.0 28-29 37.712823783391045 40.0 37.0 41.0 33.0 41.0 30-31 37.445830881951075 39.5 36.5 41.0 32.5 41.0 32-33 37.123181958469566 39.0 35.5 41.0 32.0 41.0 34-35 36.87804976064865 39.0 35.0 41.0 31.0 41.0 36-37 36.66823394384808 39.0 35.0 41.0 31.0 41.0 38-39 36.44818903131018 39.0 35.0 41.0 30.5 41.0 40-41 36.16306032168565 39.0 35.0 41.0 30.0 41.0 42-43 35.8338971512527 39.0 35.0 40.0 29.5 41.0 44-45 35.37557627576362 38.0 35.0 40.0 27.5 41.0 46-47 34.73939033721375 38.0 34.5 40.0 24.0 41.0 48-49 34.16460027198287 38.0 34.0 40.0 21.0 41.0 50-51 33.43044182749342 37.5 33.5 39.5 18.0 40.5 52-53 33.66050405241618 38.0 33.5 40.0 18.0 40.5 54-55 33.91977509974313 38.0 34.0 40.0 16.0 41.0 56-57 33.80154252223926 38.0 34.0 40.0 13.5 41.0 58-59 33.56730209709724 38.0 34.0 40.0 11.5 41.0 60-61 33.29053983455983 37.5 33.5 40.0 8.0 41.0 62-63 32.9826574751888 37.0 33.0 40.0 7.0 41.0 64-65 32.669320589360524 36.5 33.0 40.0 4.0 41.0 66-67 32.304405817089915 36.0 32.5 39.0 2.0 41.0 68-69 31.87492605666631 35.5 32.0 39.0 2.0 41.0 70-71 31.521255493507454 35.0 31.5 39.0 2.0 40.0 72-73 31.159544396542024 35.0 31.0 38.0 2.0 40.0 74-75 30.750917058084095 35.0 31.0 37.0 2.0 39.5 76-77 30.331794588633947 35.0 31.0 37.0 2.0 39.0 78-79 29.91411521978852 35.0 30.0 36.0 2.0 39.0 80-81 29.511859867737883 35.0 30.0 36.0 2.0 37.5 82-83 29.11000718536308 34.0 29.0 35.0 2.0 37.0 84-85 28.768631710759397 34.0 29.0 35.0 2.0 37.0 86-87 28.436693254117518 34.0 29.0 35.0 2.0 36.0 88-89 28.06884405672418 34.0 28.5 35.0 2.0 36.0 90-91 27.729502425662837 34.0 27.5 35.0 2.0 35.5 92-93 27.386435095435125 34.0 27.0 35.0 2.0 35.0 94-95 27.006688656771118 34.0 26.0 35.0 2.0 35.0 96-97 26.582574208565212 34.0 25.0 35.0 2.0 35.0 98-99 26.017513719703324 33.5 24.5 35.0 2.0 35.0 100-101 24.043176075152147 31.0 10.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4687539241077552 1101 2 0.2872168110084594 1101 3 0.4968056198631352 1101 4 0.301745418776747 1101 5 0.37879153034059243 1101 6 0.44888840549174347 1101 7 0.30968969525213197 1101 8 0.3282736978797729 1101 9 0.5166647591406601 1101 10-11 0.41962616620393334 1101 12-13 0.5708328517145205 1101 14-15 0.7858963303026627 1101 16-17 0.7614150373436033 1101 18-19 0.7507516371575989 1101 20-21 0.6313817787179588 1101 22-23 0.7338397138668284 1101 24-25 0.879282708844741 1101 26-27 0.8249949194630091 1101 28-29 0.8186033656524927 1101 30-31 0.7872097180409767 1101 32-33 0.6490852003118377 1101 34-35 0.5877462110350749 1101 36-37 0.5559405959490178 1101 38-39 0.7167388505766468 1101 40-41 0.7726485590041747 1101 42-43 0.6706557751266402 1101 44-45 0.7977838450205255 1101 46-47 0.7309083127282037 1101 48-49 0.763276307100611 1101 50-51 0.7801218991570735 1101 52-53 0.7191467530741633 1101 54-55 0.746559395070669 1101 56-57 0.5391157324937197 1101 58-59 0.4783022851562677 1101 60-61 0.6541974824036743 1101 62-63 0.8305967978016255 1101 64-65 0.735812988358262 1101 66-67 0.4576063744626566 1101 68-69 0.34521595139649364 1101 70-71 0.41828664452498643 1101 72-73 0.4876130992199812 1101 74-75 0.4829171286739111 1101 76-77 0.4506615133223839 1101 78-79 0.7153357840787429 1101 80-81 0.6685173070000303 1101 82-83 0.35107051498555464 1101 84-85 0.38342124226359076 1101 86-87 0.6321692459305268 1101 88-89 0.7404607934043987 1101 90-91 0.7648364181772216 1101 92-93 0.5229544719465693 1101 94-95 0.7519319291898405 1101 96-97 0.8735467025398336 1101 98-99 0.9522651314497566 1101 100-101 1.2717368966641551 1102 1 0.5433181942602623 1102 2 0.6348202968472414 1102 3 0.5646160773794726 1102 4 0.5158612502945346 1102 5 0.45942878524255804 1102 6 0.47667526097468027 1102 7 0.5663799639522011 1102 8 0.460944358736711 1102 9 0.5554265601573576 1102 10-11 0.7278687224857094 1102 12-13 0.6002809272410303 1102 14-15 0.7757066062648974 1102 16-17 0.7928420525941462 1102 18-19 0.7306853699099634 1102 20-21 0.780877966081789 1102 22-23 0.7481007886671165 1102 24-25 0.8324009005658795 1102 26-27 0.7218676500367209 1102 28-29 0.7900358275261397 1102 30-31 0.7915942496313946 1102 32-33 0.9183934791789454 1102 34-35 0.8284733352180851 1102 36-37 0.8453242197689192 1102 38-39 0.8286351831837635 1102 40-41 0.8749406795560972 1102 42-43 1.0011659421564119 1102 44-45 1.068158754243477 1102 46-47 0.9389330040862305 1102 48-49 0.9286811727505793 1102 50-51 0.8409833733763961 1102 52-53 1.0535240225004543 1102 54-55 1.0694533674744449 1102 56-57 1.0962598864515982 1102 58-59 1.1225742532027425 1102 60-61 1.1893508956208123 1102 62-63 1.2343912785568918 1102 64-65 1.2142715940227546 1102 66-67 0.9854703904394171 1102 68-69 0.976869909275937 1102 70-71 1.2201112632613729 1102 72-73 0.9864693083703138 1102 74-75 1.0972780582309198 1102 76-77 0.9906079548619644 1102 78-79 1.130422930048244 1102 80-81 1.1685808509361202 1102 82-83 1.1468039935621697 1102 84-85 1.2961209517770236 1102 86-87 1.3925042132507883 1102 88-89 1.2587478304414326 1102 90-91 1.25604919958608 1102 92-93 1.276099896783169 1102 94-95 1.3465306946219364 1102 96-97 1.2664706313706233 1102 98-99 1.2170027857707417 1102 100-101 1.3298614427788955 1103 1 0.2244356482742127 1103 2 0.32503843754937023 1103 3 0.30048932963396524 1103 4 0.2513576054235571 1103 5 0.20775343155677461 1103 6 0.13782176364513532 1103 7 0.13341249660896182 1103 8 0.16945120263694946 1103 9 0.3940226643280482 1103 10-11 0.30544977834922094 1103 12-13 0.20399658134139997 1103 14-15 0.24134691771061512 1103 16-17 0.41548759463656637 1103 18-19 0.4833719110055412 1103 20-21 0.418098395744515 1103 22-23 0.28346017386516564 1103 24-25 0.4768053571445705 1103 26-27 0.48074541586410646 1103 28-29 0.41583903207096284 1103 30-31 0.5659523754414906 1103 32-33 0.5438243829047025 1103 34-35 0.3996862085530708 1103 36-37 0.33875839606484703 1103 38-39 0.4331092278414843 1103 40-41 0.6125007762623866 1103 42-43 0.7541535413217204 1103 44-45 0.8104415557841591 1103 46-47 0.7107171983077549 1103 48-49 0.6667583539277828 1103 50-51 0.5276600599910282 1103 52-53 0.6013807648223164 1103 54-55 0.5644112193446844 1103 56-57 0.5291101479814344 1103 58-59 0.5179761082720091 1103 60-61 0.594968041682236 1103 62-63 0.5837634231015372 1103 64-65 0.660336236476077 1103 66-67 0.50500699082734 1103 68-69 0.7537468109977148 1103 70-71 0.7481234526659648 1103 72-73 0.5182596616424213 1103 74-75 0.7134313174717946 1103 76-77 0.6139935022360952 1103 78-79 0.4527664947596399 1103 80-81 0.5029230735260519 1103 82-83 0.5696297108157857 1103 84-85 0.3878195627732275 1103 86-87 0.343091621209755 1103 88-89 0.2647735881425426 1103 90-91 0.43720342405130097 1103 92-93 0.4158781709620385 1103 94-95 0.30960856524510305 1103 96-97 0.4771492300487239 1103 98-99 0.6261590877664602 1103 100-101 0.6651068395780335 1104 1 0.4886196752862766 1104 2 0.5131545031584928 1104 3 0.45692552148231513 1104 4 0.3362530022979584 1104 5 0.3541066563910107 1104 6 0.329260920351345 1104 7 0.33996997809697405 1104 8 0.34670216682832944 1104 9 0.39660429593098456 1104 10-11 0.5042849077493372 1104 12-13 0.506731601458327 1104 14-15 0.6056198904584278 1104 16-17 0.6226417231398713 1104 18-19 0.6402026004697561 1104 20-21 0.6006672368036234 1104 22-23 0.6142378487305251 1104 24-25 0.5421554799043875 1104 26-27 0.5883903386062741 1104 28-29 0.612339274650239 1104 30-31 0.7145437004144171 1104 32-33 0.7282740709820601 1104 34-35 0.7566166250543418 1104 36-37 0.6925093088000196 1104 38-39 0.715922853446088 1104 40-41 0.7368920002831274 1104 42-43 0.7840371032676501 1104 44-45 0.8171717831225962 1104 46-47 0.5527877962289196 1104 48-49 0.7211032183529156 1104 50-51 1.0545621020675142 1104 52-53 0.969436259120485 1104 54-55 0.8810080525824517 1104 56-57 0.8962406556337115 1104 58-59 0.8207571939490563 1104 60-61 0.8086335861134444 1104 62-63 0.6751704809334171 1104 64-65 0.6854466222996436 1104 66-67 0.6101440358686858 1104 68-69 0.5830131434440382 1104 70-71 0.46882722661147724 1104 72-73 0.5623568991789902 1104 74-75 0.611739633285513 1104 76-77 0.735739141934804 1104 78-79 0.6870564235540435 1104 80-81 0.6950416972439335 1104 82-83 0.7030637513044731 1104 84-85 0.7913967495396719 1104 86-87 0.8794983605031845 1104 88-89 0.8356890676409208 1104 90-91 0.6670499865313921 1104 92-93 0.6160511618420372 1104 94-95 0.7107631446028329 1104 96-97 0.6315798548251372 1104 98-99 0.8590403415337917 1104 100-101 1.0851335968076263 1105 1 0.3216013209501618 1105 2 0.3130031171216565 1105 3 0.37759728481222865 1105 4 0.32359761796055153 1105 5 0.30106831421461777 1105 6 0.27870775088933186 1105 7 0.2359433536881781 1105 8 0.27336881721692663 1105 9 0.37593863687675366 1105 10-11 0.4061528161070669 1105 12-13 0.10813241064110457 1105 14-15 0.16373161823703697 1105 16-17 0.2914420376321587 1105 18-19 0.4068671509875159 1105 20-21 0.3038690514826996 1105 22-23 0.23399274985347063 1105 24-25 0.3357826160956634 1105 26-27 0.24158592692415937 1105 28-29 0.3880987075874458 1105 30-31 0.20897122923269507 1105 32-33 0.06330724161610135 1105 34-35 0.016524963250752478 1105 36-37 0.17866798578372567 1105 38-39 0.10529335612098123 1105 40-41 0.2984480045707656 1105 42-43 0.27430957371684883 1105 44-45 -0.01626377648076982 1105 46-47 0.32636360767028805 1105 48-49 0.39005760476333506 1105 50-51 0.2215052517272582 1105 52-53 0.23354604818113245 1105 54-55 0.4112972243299211 1105 56-57 0.5527833403918194 1105 58-59 0.5477564052766368 1105 60-61 0.512980562909874 1105 62-63 0.33293304414318925 1105 64-65 0.5288428947847379 1105 66-67 0.5925957083181288 1105 68-69 0.605398254871492 1105 70-71 0.5726638859611555 1105 72-73 0.44960555562791527 1105 74-75 0.6343176398244132 1105 76-77 0.2938503813713602 1105 78-79 0.526726401993745 1105 80-81 0.6260943917933055 1105 82-83 0.5821837100884046 1105 84-85 0.6025878402272333 1105 86-87 0.6349205444176889 1105 88-89 0.7344689450182322 1105 90-91 0.84158319257023 1105 92-93 0.8009108489612835 1105 94-95 0.7801106463669676 1105 96-97 0.8767524715327824 1105 98-99 0.9284566995275831 1105 100-101 1.1269251773022688 1106 1 0.4264706234807498 1106 2 0.35622796306895665 1106 3 0.38212007925144675 1106 4 0.2733764674533319 1106 5 0.2884783871826002 1106 6 0.40038472004685843 1106 7 0.39083708911610415 1106 8 0.3111836437946778 1106 9 0.5285287805832439 1106 10-11 0.5864547690852859 1106 12-13 0.6359000991179613 1106 14-15 0.8569054601547634 1106 16-17 0.7964494986464317 1106 18-19 0.7365456231816339 1106 20-21 0.6985732423660664 1106 22-23 0.7993873853357414 1106 24-25 0.7260672180104919 1106 26-27 0.8567672235840007 1106 28-29 0.6933802047852211 1106 30-31 0.4614827999344371 1106 32-33 0.6507741631223496 1106 34-35 0.5213821776267622 1106 36-37 0.4714125841747432 1106 38-39 0.4772259156223555 1106 40-41 0.5811887997546634 1106 42-43 0.4365468630508147 1106 44-45 0.39290571598209567 1106 46-47 0.32840747512709356 1106 48-49 0.5306200346648708 1106 50-51 0.516415454414819 1106 52-53 0.39165369319764665 1106 54-55 0.41059160681623297 1106 56-57 0.2931691235816487 1106 58-59 0.4603159978830149 1106 60-61 0.5268237806853904 1106 62-63 0.5564966207088204 1106 64-65 0.4211519774933734 1106 66-67 0.42609791469143943 1106 68-69 0.4721885020115302 1106 70-71 0.6028825618178644 1106 72-73 0.7022459906190157 1106 74-75 0.6834519968115629 1106 76-77 0.7479946512154605 1106 78-79 0.6808589258008517 1106 80-81 0.8424290238429926 1106 82-83 1.05302930501092 1106 84-85 1.0849642231662848 1106 86-87 1.0517335138671555 1106 88-89 0.9393434933517462 1106 90-91 0.8638695871811919 1106 92-93 0.9377276615315964 1106 94-95 1.0054672041623185 1106 96-97 0.8904725269786411 1106 98-99 0.8883092012338949 1106 100-101 1.237710548126369 1107 1 0.15189806518199944 1107 2 0.1632933966028709 1107 3 0.2186131335999839 1107 4 0.12035884894243765 1107 5 0.17045092038191 1107 6 0.16420605681005895 1107 7 0.17196820187563588 1107 8 0.1755470931176646 1107 9 0.26441255485561044 1107 10-11 0.34110037500403934 1107 12-13 0.15442743364577893 1107 14-15 0.06742053484327926 1107 16-17 0.12838164295760635 1107 18-19 0.1596302736954911 1107 20-21 0.16758662814505243 1107 22-23 0.19072890328625647 1107 24-25 0.07467900096042968 1107 26-27 0.2384578299711677 1107 28-29 0.1463246460545946 1107 30-31 0.29642245816961577 1107 32-33 0.4006116943272602 1107 34-35 0.5907962706679157 1107 36-37 0.5173115654084981 1107 38-39 0.5766317776968108 1107 40-41 0.7833603726941689 1107 42-43 0.8493354403308828 1107 44-45 0.8667846223770894 1107 46-47 0.7807208952557829 1107 48-49 1.2660242093027634 1107 50-51 1.0189271660140022 1107 52-53 1.0600202675777197 1107 54-55 1.0523533636356532 1107 56-57 1.2640779295376419 1107 58-59 1.318141808945505 1107 60-61 1.0839385587762962 1107 62-63 1.1641157738207042 1107 64-65 1.3651560688199922 1107 66-67 1.2108646165719534 1107 68-69 1.1995004638980937 1107 70-71 1.319609747987954 1107 72-73 1.2122127073470885 1107 74-75 1.4191089334189826 1107 76-77 1.2712148740584261 1107 78-79 1.336159356085389 1107 80-81 1.2808543815836657 1107 82-83 1.283460726682108 1107 84-85 1.129536056762884 1107 86-87 1.107532633501343 1107 88-89 0.9365380711656108 1107 90-91 1.1505926902733137 1107 92-93 1.1187591424751702 1107 94-95 1.0492660221318637 1107 96-97 0.8932119600258801 1107 98-99 1.1196382519506685 1107 100-101 1.1046281306378312 1108 1 -0.023840800614873814 1108 2 0.18197293409826543 1108 3 0.11919938163830679 1108 4 0.18467141835807155 1108 5 0.11740101327967523 1108 6 0.06554373310321893 1108 7 0.19500736324459922 1108 8 0.0681077987799199 1108 9 -0.012710371694595324 1108 10-11 0.011577181485499466 1108 12-13 -0.1663138931044017 1108 14-15 0.20488706716680127 1108 16-17 0.26484292095256734 1108 18-19 0.30941162250405796 1108 20-21 0.16295101673344448 1108 22-23 0.19819757444878405 1108 24-25 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2316 62-63 -1.5091869965108025 2316 64-65 -1.6146350054087826 2316 66-67 -1.5941101233947634 2316 68-69 -1.1843516372283638 2316 70-71 -1.7434342358801587 2316 72-73 -1.5623771617850828 2316 74-75 -1.5116387731570171 2316 76-77 -1.7370551793149325 2316 78-79 -2.1325362455278025 2316 80-81 -2.1045787194900356 2316 82-83 -2.2593794265804767 2316 84-85 -1.776088886291145 2316 86-87 -1.74227806610687 2316 88-89 -2.179535797947377 2316 90-91 -2.1999960711098865 2316 92-93 -2.385199501563367 2316 94-95 -2.35073655542255 2316 96-97 -2.2798935974212142 2316 98-99 -2.4933011911057577 2316 100-101 -2.062734275910838 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2513.0 3 649.0 4 696.0 5 734.0 6 869.0 7 1032.0 8 1358.0 9 1909.0 10 2392.0 11 3565.0 12 5460.0 13 7615.0 14 8900.0 15 12238.0 16 16621.0 17 23603.0 18 19168.0 19 10017.0 20 8334.0 21 8307.0 22 8407.0 23 8900.0 24 9975.0 25 11027.0 26 12967.0 27 15215.0 28 18127.0 29 21892.0 30 26490.0 31 33539.0 32 43239.0 33 56880.0 34 78754.0 35 116173.0 36 165891.0 37 232239.0 38 208975.0 39 39509.0 40 17.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.524034235707845 19.283279332398873 15.172292333636511 20.020394098256773 2 15.216940104355213 21.606162846261892 40.95065250164513 22.226244547737764 3 18.098584582818273 28.553160379900255 31.293113362860396 22.055141674421076 4 11.668113781008012 17.78469996451284 38.5754279180142 31.97175833646495 5 11.963629459429887 39.12222522574707 33.22568914805017 15.688456166772863 6 27.849929243025066 38.170149808485526 18.408318027461267 15.571602921028136 7 25.760647781618296 36.395717071852005 21.130624802878454 16.713010343651238 8 26.79068330626302 32.8780854327912 20.24738970555702 20.083841555388762 9 28.532611756678687 15.699683143525201 20.838824814898853 34.928880284897254 10-11 23.898583472084386 28.017267215019952 26.760442131457555 21.323707181438106 12-13 26.494868084141054 26.236200861875485 27.82581169862835 19.44311935535511 14-15 23.28298835965216 25.855690255733695 28.63626125220217 22.225060132411972 16-17 19.753789166385406 29.76924874800721 28.68112430541504 21.795837780192347 18-19 19.74973916918649 26.384896990933466 34.20583955345443 19.65952428642562 20-21 21.583367984705674 25.147928518173266 34.20134685929881 19.067356637822247 22-23 20.825598715704945 25.583997143151283 34.13672820044268 19.45367594070109 24-25 20.048550638388217 25.808158630384643 34.56267431424334 19.580616416983794 26-27 19.896640930770413 26.055202572678244 34.29389262333247 19.754263873218882 28-29 19.674489827103383 25.91973472778715 34.57723246383711 19.82854298127235 30-31 19.811268032603323 25.956173753061133 34.4995297983983 19.73302841593724 32-33 19.513724909968786 26.215778188504068 34.12702592842614 20.143470973101003 34-35 19.918675326074634 26.155603996921982 34.00797004318715 19.917750633816237 36-37 20.24363737681533 25.920615819824615 33.627771717913284 20.20797508544677 38-39 19.934134266866785 26.205652101716765 33.74977534807479 20.110438283341665 40-41 19.89422036071074 26.25676151689595 33.7334751985228 20.115542923870507 42-43 19.97686296554536 26.47525736633477 33.14506848224432 20.402811185875553 44-45 20.000972974233473 26.818667757041098 32.72976766420649 20.45059160451894 46-47 20.445428192281607 27.02711394675621 31.772647584620785 20.754810276341395 48-49 20.53302746429015 27.398356235318143 31.00168493317557 21.066931367216128 50-51 20.769825069722085 27.85752013679919 30.369806418210306 21.002848375268417 52-53 20.796774500900327 28.14347665802722 30.145704661318977 20.91404417975347 54-55 20.521668580397606 28.131482964578286 30.079267057132103 21.267581397892002 56-57 20.627899328324784 28.565696397656172 29.600187197402395 21.20621707661665 58-59 20.75621000758333 27.869623012793554 30.075101506453066 21.29906547317005 60-61 20.7232197136617 27.899328060316957 30.427424724421098 20.95002750160024 62-63 20.898514699206384 27.858587816263235 30.508156307411703 20.73474117711867 64-65 20.799591866106997 28.000389158400736 30.362435922536573 20.837583052955694 66-67 20.688092503771756 28.321237972997753 29.935413670974032 21.05525585225646 68-69 20.788640133936866 28.265977575868206 30.001182079672166 20.944200210522762 70-71 20.664945455451704 28.411722405048994 29.904014940209816 21.01931719928949 72-73 20.682519942537837 28.474881204936857 29.47311344757703 21.369485404948275 74-75 20.546165469630026 28.876929465351324 29.324215300174338 21.252689764844305 76-77 20.601455212689572 28.73836002724202 29.476459534586024 21.183725225482384 78-79 20.50007479166901 28.702589721855414 29.411140259309953 21.386195227165622 80-81 20.28256394031286 29.05866560640531 29.253444408843322 21.405326044438514 82-83 20.425210578715504 28.941324253424668 29.011128463873103 21.622336703986722 84-85 20.426959831274868 28.662081278386413 28.997762260820263 21.913196629518453 86-87 20.52528107169756 28.753818630291445 28.832030934273114 21.888869363737882 88-89 20.577444395286168 28.548807464907693 28.817238466798052 22.056509673008083 90-91 20.20414490395724 28.73670864595971 29.111679341949547 21.947467108133505 92-93 20.166756681869384 28.710734302870122 29.065030923539894 22.057478091720597 94-95 20.066013847866515 28.572163986538225 29.040462539333216 22.32135962626204 96-97 20.343849106993826 28.51630859944535 28.75662891928254 22.383213374278284 98-99 20.17469251783336 28.510647714151222 28.731246303575524 22.583413464439893 100-101 20.216207822135253 28.43638560963992 28.943252185331563 22.404154382893264 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1698.0 1 1224.0 2 955.0 3 1191.5 4 1078.0 5 1129.5 6 1920.5 7 3988.0 8 6626.5 9 7444.0 10 5966.5 11 4638.0 12 4483.5 13 4529.5 14 4623.0 15 4752.5 16 4801.0 17 4864.0 18 4957.5 19 5075.0 20 5349.0 21 5866.0 22 6640.0 23 7507.0 24 8437.5 25 9748.0 26 11464.0 27 13160.0 28 14396.5 29 15849.5 30 18284.5 31 20787.5 32 23814.0 33 26466.0 34 29261.5 35 31503.0 36 34285.5 37 38049.0 38 38444.5 39 37072.5 40 36382.5 41 36787.0 42 36896.0 43 37720.5 44 39011.5 45 39257.0 46 38605.5 47 38305.0 48 41310.5 49 41180.0 50 37230.0 51 34714.5 52 32830.5 53 32534.5 54 32164.5 55 29991.5 56 27355.5 57 25072.5 58 24035.5 59 22613.5 60 20034.5 61 17376.0 62 14380.0 63 10566.0 64 7174.0 65 5363.0 66 3837.0 67 2892.0 68 2077.5 69 1295.0 70 929.0 71 663.0 72 535.0 73 472.5 74 362.0 75 263.0 76 187.0 77 102.0 78 61.0 79 37.0 80 23.0 81 18.5 82 17.0 83 13.0 84 6.5 85 4.0 86 5.5 87 6.0 88 4.5 89 3.5 90 2.5 91 0.5 92 1.5 93 1.5 94 0.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05618085896434324 2 0.09114319608807615 3 0.06566489524158574 4 0.11999717086375458 5 0.09741230481371102 6 0.09717118524734045 7 0.10512813093756933 8 0.09138431565444673 9 0.10946828313223962 10-11 0.10147115084761565 12-13 0.08784789534767834 14-15 0.08889274680195082 16-17 0.10267674867946851 18-19 0.08463296779607071 20-21 0.06904056917077374 22-23 0.06976392786988544 24-25 0.060440637970223345 26-27 0.05360891692305714 28-29 0.06381631189941135 30-31 0.04665663609270565 32-33 0.04818372667971927 34-35 0.043321148757912736 36-37 0.0470585020366566 38-39 0.04866596581246042 40-41 0.061244369858125244 42-43 0.059074293760790104 44-45 0.0471388752254468 46-47 0.034279165019016296 48-49 0.042316483898035356 50-51 0.02644277911197271 52-53 0.03869969040247678 54-55 0.044366000212185217 56-57 0.04733980819742227 58-59 0.054573395188539424 60-61 0.05079585531540047 62-63 0.05710515063543044 64-65 0.039101556346427736 66-67 0.05979765245990182 68-69 0.05023324299386913 70-71 0.02447363598661304 72-73 0.01985217763117708 74-75 0.02897453455886372 76-77 0.042517416870010837 78-79 0.05963690608232143 80-81 0.038378197647316016 82-83 0.057707949551356864 84-85 0.060561197753408624 86-87 0.06313313979469473 88-89 0.08407035547453938 90-91 0.06152567601889091 92-93 0.07133120505129417 94-95 0.07812273950406527 96-97 0.08547688627836772 98-99 0.11581776504666468 100-101 0.10962902950982 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1244196.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.570612135406684 #Duplication Level Percentage of deduplicated Percentage of total 1 74.39882081615575 36.13596269193122 2 13.61469367295557 13.225480114630006 3 4.837503155834789 7.04881468457572 4 2.2790045378360415 4.427705818482646 5 1.2228011308240567 2.9696099721995974 6 0.7310360761321297 2.1304121826481985 7 0.5268880273418572 1.7913891810367628 8 0.3541817462314009 1.3762259377317128 9 0.25756792214419955 1.1259208480489686 >10 1.5664353759636231 14.21779247213487 >50 0.1384230554901546 4.616639535386942 >100 0.06882982160389967 5.914235449949865 >500 0.0019902581669225634 0.7238826370580358 >1k 0.0013268387779483755 1.077365500557998 >5k 1.6585484724354694E-4 0.7279910295007199 >10k+ 3.317096944870939E-4 2.4905719441267853 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18252 1.466971441798559 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12665 1.0179264360277642 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9037 0.7263325070969525 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2672 0.21475716044738932 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2309 0.18558169291655013 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1915 0.153914656533215 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1765 0.14185867821468642 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1288 0.1035206671617655 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 8.037318879019062E-5 0.0 0.0 0.0 0.0 9 8.037318879019062E-5 0.0 0.0 0.0 0.0 10-11 8.037318879019062E-5 0.0 0.0 0.0 0.0 12-13 8.037318879019062E-5 0.0 0.0 1.6074637758038124E-4 0.0 14-15 8.037318879019062E-5 0.0 0.0 1.6074637758038124E-4 0.0 16-17 8.037318879019062E-5 0.0 0.0 1.6074637758038124E-4 0.0 18-19 8.037318879019062E-5 0.0 0.0 1.6074637758038124E-4 0.0 20-21 8.037318879019062E-5 0.0 0.0 2.8130616076566716E-4 0.0 22-23 8.037318879019062E-5 0.0 0.0 6.831721047166203E-4 0.0 24-25 8.037318879019062E-5 0.0 0.0 0.0012859710206430499 0.0 26-27 8.037318879019062E-5 0.0 0.0 0.0018083967477792889 0.0 28-29 8.037318879019062E-5 0.0 0.0 0.003415860523583101 0.0 30-31 8.037318879019062E-5 0.0 0.0 0.007836385907043586 0.0 32-33 8.037318879019062E-5 0.0 0.0 0.012216724696108973 0.0 34-35 8.037318879019062E-5 0.0 0.0 0.017240048995495888 0.0 36-37 8.037318879019062E-5 0.0 0.0 0.02346897112673566 0.0 38-39 8.037318879019062E-5 0.0 0.0 0.033274500159138916 0.0 40-41 8.037318879019062E-5 0.0 0.0 0.05497526113249038 0.0 42-43 8.037318879019062E-5 0.0 0.0 0.07350128114862932 0.0 44-45 8.037318879019062E-5 0.0 0.0 0.0976534243800816 0.0 46-47 8.037318879019062E-5 0.0 0.0 0.140411960816463 0.0 48-49 1.6074637758038124E-4 0.0 0.0 0.17360608778681172 0.0 50-51 1.6074637758038124E-4 0.0 0.0 0.21909731264205962 0.0 52-53 1.6074637758038124E-4 0.0 0.0 0.26880812990879255 0.0 54-55 1.6074637758038124E-4 0.0 0.0 0.3298113802005472 0.0 56-57 2.4111956637057184E-4 8.037318879019062E-5 0.0 0.4326890618519912 0.0 58-59 2.4111956637057184E-4 8.037318879019062E-5 0.0 0.5276901710019964 0.0 60-61 2.4111956637057184E-4 8.037318879019062E-5 0.0 0.6190343000620481 0.0 62-63 2.4111956637057184E-4 8.037318879019062E-5 0.0 0.7211886230143804 0.0 64-65 2.4111956637057184E-4 8.037318879019062E-5 0.0 0.8211728698693774 0.0 66-67 2.4111956637057184E-4 8.037318879019062E-5 0.0 0.9559587074705271 0.0 68-69 2.8130616076566716E-4 8.037318879019062E-5 0.0 1.1279171448871401 0.0 70-71 3.2149275516076247E-4 8.037318879019062E-5 0.0 1.2601712270413987 0.0 72-73 3.2149275516076247E-4 8.037318879019062E-5 0.0 1.410388716890265 0.0 74-75 4.0186594395095305E-4 8.037318879019062E-5 0.0 1.6024002649100302 0.0 76-77 4.822391327411437E-4 8.037318879019062E-5 0.0 1.8208144054473732 0.0 78-79 4.822391327411437E-4 8.037318879019062E-5 0.0 2.059000350427103 0.0 80-81 4.822391327411437E-4 8.037318879019062E-5 0.0 2.303093724782912 0.0 82-83 4.822391327411437E-4 8.037318879019062E-5 0.0 2.5798186137875385 0.0 84-85 4.822391327411437E-4 8.037318879019062E-5 0.0 2.925423325585358 0.0 86-87 4.822391327411437E-4 8.037318879019062E-5 0.0 3.2494478361930117 0.0 88-89 4.822391327411437E-4 8.037318879019062E-5 0.0 3.6369671659449154 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6770 0.0 50.6882 1 GTATCAA 12435 0.0 43.649433 1 TATCAAC 16145 0.0 33.50272 2 ATCAACG 16225 0.0 33.3335 3 TCAACGC 16405 0.0 32.969086 4 CAACGCA 16610 0.0 32.50103 5 AACGCAG 17005 0.0 31.884525 6 GTCGTAT 65 0.005826869 29.248592 1 ACGCAGA 18955 0.0 28.37877 7 CGCAGAG 18970 0.0 28.283459 8 AGGATCG 85 6.3906895E-4 27.953712 5 GCAGAGT 19455 0.0 27.04206 9 GTACATG 10820 0.0 26.487953 1 TACATGG 11000 0.0 25.623465 2 GAGTACT 11560 0.0 24.910185 12-13 ACATGGG 11150 0.0 24.082243 3 AGTACTT 11780 0.0 24.02142 12-13 GTACTTT 12345 0.0 23.209776 14-15 ATGGGAT 2965 0.0 22.278118 5 CATGGGA 8140 0.0 22.245462 4 >>END_MODULE