FastQCFastQC Report
Thu 26 May 2016
SRR936967_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936967_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1244196
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184831.485537648409093No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143311.1518281685522218No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107960.8677089461788979No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31460.25285405193393967No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29740.2390298634620269No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26330.21162260608457187No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22200.17842847911422316No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14910.1198364244861742No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14610.11742522882246847No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13680.10995052226498077No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72500.053.33881
GTATCAA133350.042.7865071
ATCAACG182300.031.21333
TATCAAC183150.031.1487452
TCAACGC184350.030.840444
CAACGCA187750.030.3072435
AACGCAG191600.029.7490256
ACGCAGA211150.026.8146517
CGCAGAG212250.026.5861688
GCAGAGT218050.025.5958029
AGTACTT140100.024.56283812-13
CTACACG1551.4114739E-624.514774
GAGTACT138750.024.13428112-13
GTACATG103950.023.673481
CATGGGG27950.023.1114224
TACATGG105850.023.0662362
GTACTTT147650.022.48831414-15
AGAGTAC209450.022.3039110-11
ACATGGG105750.021.8734933
TACTTTT157100.021.74031814-15
Produced by FastQC (version 0.11.5)