FastQCFastQC Report
Thu 26 May 2016
SRR936966_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936966_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1071476
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160431.4972803870548663No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112191.047060316796643No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81230.7581131075264402No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23130.21587044413500628No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20250.18899163397033625No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15600.1455935550586294No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15020.14018046134491113No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11830.11040844591946064No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10740.1002355629057487No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57800.050.5634961
GTATCAA105500.042.701751
TATCAAC139900.031.9980132
ATCAACG140200.031.8956493
TCAACGC142050.031.4467984
CAACGCA145300.030.7121415
AACGCAG147200.030.3465886
CGCGTGC650.00582967929.2453429
ACGCAGA166050.026.7299297
CGCAGAG166450.026.5527368
GCAGAGT171000.025.3181729
GTACATG91600.024.5908031
GAGTACT100800.023.96952212-13
TACATGG93900.023.8872532
AGTACTT102600.023.5721612-13
ATGGGAG25000.022.8113695
ACATGGG92250.022.6660633
GTACTTT108750.021.97901514-15
AGAGTAC164700.021.17687210-11
TGGGACG1805.148022E-621.1206516
Produced by FastQC (version 0.11.5)