FastQCFastQC Report
Thu 26 May 2016
SRR936966_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936966_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1071476
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163231.5234125636038511No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128041.194987101904289No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91190.8510689926792573No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26600.24825567721535527No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25420.23724283138399743No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23070.21531046892324232No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20290.1893649507781789No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13260.12375452179983498No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12670.11824809888415606No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12140.11330165118024109No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA11250.10499535220574235No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67950.048.9523281
GTATCAA118250.039.582191
ATAGCGC550.002581649234.539726
ATCAACG156400.029.8247853
TCAACGC157850.029.4564134
TATCAAC158250.029.4188352
CAACGCA161000.028.7915945
GTAAGGT4000.028.49664
AACGCAG164950.028.1296126
ACGCAGA182850.025.2719867
CGCAGAG183350.025.1253628
CGTCACT1901.1857992E-825.0098551
TAAGGTG4500.024.274885
GAGTACT113950.024.15410612-13
GCAGAGT189500.024.0092079
AGTACTT116250.023.51279612-13
GTACATG91250.023.4338911
TACATGG94500.022.5210862
GTACTTT123050.022.29270714-15
ACATGGG91750.022.2102663
Produced by FastQC (version 0.11.5)