##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936966_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1071476 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.07645341566214 31.0 31.0 34.0 31.0 34.0 2 32.12326174361348 33.0 31.0 34.0 30.0 34.0 3 32.21676920434989 33.0 31.0 34.0 30.0 34.0 4 35.978607080326576 37.0 35.0 37.0 35.0 37.0 5 35.90093571857886 37.0 35.0 37.0 35.0 37.0 6 35.96507154616622 37.0 35.0 37.0 35.0 37.0 7 35.91485576905129 37.0 35.0 37.0 35.0 37.0 8 35.901433163225306 37.0 35.0 37.0 35.0 37.0 9 37.539432521120396 39.0 37.0 39.0 35.0 39.0 10-11 37.44513456204339 39.0 37.0 39.0 34.5 39.0 12-13 37.35245306474434 39.0 37.0 39.0 34.0 39.0 14-15 38.41763464603967 40.0 38.0 41.0 34.0 41.0 16-17 38.367657325035744 40.0 38.0 41.0 33.5 41.0 18-19 38.5138327876686 40.0 38.0 41.0 34.0 41.0 20-21 38.63914777372521 40.0 38.0 41.0 34.0 41.0 22-23 38.56054498654193 40.0 38.0 41.0 34.0 41.0 24-25 38.449687160515026 40.0 38.0 41.0 34.0 41.0 26-27 38.236097682075936 40.0 37.5 41.0 34.0 41.0 28-29 38.03522990715611 40.0 37.0 41.0 33.0 41.0 30-31 37.807808107694434 40.0 37.0 41.0 33.0 41.0 32-33 37.54108864780919 40.0 35.5 41.0 33.0 41.0 34-35 37.33840188674314 40.0 35.0 41.0 33.0 41.0 36-37 37.07508054310129 39.0 35.0 41.0 32.5 41.0 38-39 36.856170833504436 39.0 35.0 41.0 31.5 41.0 40-41 36.53031799125692 39.0 35.0 41.0 31.0 41.0 42-43 36.14678350238363 39.0 35.0 41.0 30.0 41.0 44-45 35.64608026684685 39.0 35.0 40.5 29.0 41.0 46-47 34.943005722946666 38.5 35.0 40.0 25.0 41.0 48-49 34.302793996319096 38.0 34.0 40.0 21.0 41.0 50-51 33.9585203028346 38.0 34.0 40.0 18.5 41.0 52-53 33.68645214638499 38.0 34.0 40.0 14.5 41.0 54-55 33.38365208366776 38.0 33.0 40.0 11.0 41.0 56-57 33.12025747660237 38.0 33.0 40.0 8.0 41.0 58-59 32.770920673911505 37.0 33.0 40.0 6.5 41.0 60-61 32.42901007582064 36.5 32.0 40.0 2.0 41.0 62-63 32.07492048351993 36.0 32.0 39.0 2.0 41.0 64-65 31.64907426764575 35.5 31.0 39.0 2.0 40.0 66-67 31.21206074611097 35.0 31.0 39.0 2.0 40.0 68-69 30.77265566377595 35.0 30.5 38.0 2.0 40.0 70-71 30.382931115582615 35.0 30.0 37.5 2.0 40.0 72-73 30.016523935207132 35.0 29.5 37.0 2.0 39.0 74-75 29.54334301468255 34.0 29.0 36.5 2.0 39.0 76-77 28.13548693577831 32.5 27.0 35.0 2.0 37.5 78-79 28.807330262180393 34.0 29.0 35.0 2.0 37.5 80-81 28.517929006342655 34.0 29.0 35.0 2.0 37.0 82-83 28.32109258630151 34.0 28.5 35.0 2.0 37.0 84-85 27.987699211181585 34.0 27.5 35.0 2.0 36.0 86-87 27.590695918527338 34.0 27.0 35.0 2.0 36.0 88-89 27.198067432214998 33.0 26.0 35.0 2.0 35.5 90-91 26.73623347606479 33.0 25.0 35.0 2.0 35.0 92-93 26.308485677700666 33.0 25.0 35.0 2.0 35.0 94-95 25.805546741130925 33.0 23.5 35.0 2.0 35.0 96-97 25.15209160074514 32.0 19.0 34.0 2.0 35.0 98-99 24.364499064841397 32.0 6.0 34.0 2.0 35.0 100-101 21.406590068279645 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.28466693917481223 1101 2 0.28373190728077446 1101 3 0.19368643777727357 1101 4 0.14251439918518827 1101 5 0.2448534256906072 1101 6 0.275601189257344 1101 7 0.13727476053997378 1101 8 0.19180702923335247 1101 9 0.2634427300862683 1101 10-11 0.35265389810923153 1101 12-13 0.1673481856146779 1101 14-15 0.32252862974235796 1101 16-17 0.3450774728445296 1101 18-19 0.33256733379479897 1101 20-21 0.2599962813069965 1101 22-23 0.23607325474843321 1101 24-25 0.3117471625556192 1101 26-27 0.2102560667181237 1101 28-29 0.38252481179326026 1101 30-31 0.24996957507022444 1101 32-33 0.33826662462693236 1101 34-35 0.1658110160080426 1101 36-37 0.4271836301842953 1101 38-39 0.45039572963431596 1101 40-41 0.4243134445594805 1101 42-43 0.5189928498323795 1101 44-45 0.5561465397383714 1101 46-47 0.697933239353766 1101 48-49 0.6287559273567425 1101 50-51 0.49374065493696406 1101 52-53 0.41180200047158166 1101 54-55 0.3628089187670227 1101 56-57 0.5715055678974394 1101 58-59 0.5602459788071954 1101 60-61 0.5289705949589987 1101 62-63 0.6946271029741347 1101 64-65 0.9351761825035396 1101 66-67 0.6630780774403213 1101 68-69 0.5848859222739513 1101 70-71 0.7961034629809873 1101 72-73 0.8223584259897336 1101 74-75 0.7296624854988352 1101 76-77 0.5429473002221528 1101 78-79 0.8971054943980796 1101 80-81 1.0771705875097304 1101 82-83 1.0643945057718902 1101 84-85 0.7587499686319781 1101 86-87 0.6705826065157368 1101 88-89 0.3860306142136274 1101 90-91 0.30206696265027233 1101 92-93 0.587204923405455 1101 94-95 0.7791931035765955 1101 96-97 0.6869492209966133 1101 98-99 0.8200791294395557 1101 100-101 0.9790424253393866 1102 1 0.1769075884338065 1102 2 0.14592114006633494 1102 3 0.143858834188201 1102 4 0.1568233012922562 1102 5 0.14591351239289452 1102 6 0.25241034340594837 1102 7 0.04013744831092225 1102 8 0.08636696471072014 1102 9 0.09034161102184868 1102 10-11 0.1346755232730601 1102 12-13 0.19463546255791897 1102 14-15 0.1944174290164824 1102 16-17 0.20506613097538917 1102 18-19 0.16300059319594595 1102 20-21 0.10435407947213804 1102 22-23 0.24792979270588944 1102 24-25 0.3043192464350426 1102 26-27 0.2759746371385319 1102 28-29 0.2020282899664565 1102 30-31 0.3919443205082729 1102 32-33 0.3081789141722453 1102 34-35 0.3700271668296935 1102 36-37 0.41546344669804824 1102 38-39 0.2781959111042269 1102 40-41 0.43209875557873545 1102 42-43 0.5097872847552551 1102 44-45 0.29240321883909104 1102 46-47 0.6104430689736873 1102 48-49 0.8530474385977982 1102 50-51 0.853486849109764 1102 52-53 0.681546934436696 1102 54-55 0.8445134756779993 1102 56-57 0.7946964137488308 1102 58-59 0.8721602342764996 1102 60-61 0.9456741437038332 1102 62-63 0.8505508352052011 1102 64-65 0.8378800841065157 1102 66-67 0.7160703145610015 1102 68-69 0.6886597401991459 1102 70-71 0.8732780773574369 1102 72-73 1.0321681346297176 1102 74-75 0.8090374224884513 1102 76-77 0.8846712643314376 1102 78-79 0.8695384513493849 1102 80-81 0.9167872323327977 1102 82-83 1.0787281079495266 1102 84-85 1.0269239277004338 1102 86-87 1.077851232385278 1102 88-89 1.0634809620309476 1102 90-91 0.924279887340262 1102 92-93 1.1949645261879915 1102 94-95 1.4807363539042484 1102 96-97 1.3313286947338874 1102 98-99 1.399956637252842 1102 100-101 1.629986320893373 1103 1 0.21383124512382068 1103 2 0.10637253860411988 1103 3 0.17692804036731502 1103 4 0.17493851897474855 1103 5 0.21896714483265356 1103 6 0.23896824712864628 1103 7 0.1858198839717815 1103 8 0.11674025464816395 1103 9 0.24051274222710362 1103 10-11 0.1699758325487366 1103 12-13 0.2932435720978859 1103 14-15 0.383793299916384 1103 16-17 0.2593520580327109 1103 18-19 0.39260679150422817 1103 20-21 0.3307328252163515 1103 22-23 0.25780332354649715 1103 24-25 0.1627801451537607 1103 26-27 0.2713699882074039 1103 28-29 0.2583928814007024 1103 30-31 0.4197803804121918 1103 32-33 0.2979145404504848 1103 34-35 0.30187131548193946 1103 36-37 0.4899527519973219 1103 38-39 0.4097947583676316 1103 40-41 0.5544743106056131 1103 42-43 0.6703829752876658 1103 44-45 0.6232732091030044 1103 46-47 1.0153310540037026 1103 48-49 1.0195804923101974 1103 50-51 0.9280901085994486 1103 52-53 0.7478602764732045 1103 54-55 0.8565351429677506 1103 56-57 0.7203198131422752 1103 58-59 0.7545590099686734 1103 60-61 0.5407239336882625 1103 62-63 0.7871878071424838 1103 64-65 0.6334380198163672 1103 66-67 0.7549619301315431 1103 68-69 0.6193830894127608 1103 70-71 0.5889828640331949 1103 72-73 0.7800364915502378 1103 74-75 0.5000004053693345 1103 76-77 0.6888052524682102 1103 78-79 0.8098179589873133 1103 80-81 1.061708224094108 1103 82-83 1.0222860476577615 1103 84-85 0.8186579006433092 1103 86-87 0.8374796117767644 1103 88-89 0.7030763446871227 1103 90-91 0.7710349917882731 1103 92-93 0.7444014025636925 1103 94-95 0.7882349894527572 1103 96-97 1.1175036524009023 1103 98-99 1.221584592814704 1103 100-101 1.1326897341212572 1104 1 0.05096004262308895 1104 2 0.12277952632839373 1104 3 0.08169546897595836 1104 4 0.12154559787155961 1104 5 -0.005840334572120298 1104 6 0.07647557349379497 1104 7 -0.006185830593032904 1104 8 0.011967127755518447 1104 9 0.1479624345197692 1104 10-11 0.07700324129478275 1104 12-13 0.0715559031844677 1104 14-15 0.10497321102315738 1104 16-17 0.09461975527474209 1104 18-19 0.10518432886868823 1104 20-21 0.06572903992768886 1104 22-23 -0.12009669599048323 1104 24-25 0.002044781603231627 1104 26-27 0.0266866906918537 1104 28-29 -0.011381181638597582 1104 30-31 0.021989279503713988 1104 32-33 0.0696972486006544 1104 34-35 0.04015625410328738 1104 36-37 0.15389340029923204 1104 38-39 0.22042857035682317 1104 40-41 0.12891730334437312 1104 42-43 0.10914473817392434 1104 44-45 0.06786452003394317 1104 46-47 0.12086632638168027 1104 48-49 0.23646939205296746 1104 50-51 0.25916142669394304 1104 52-53 0.4248459249379195 1104 54-55 0.3914768169607825 1104 56-57 0.2874415284885714 1104 58-59 0.2861224155888138 1104 60-61 0.2822772452053002 1104 62-63 0.39017513253078207 1104 64-65 0.2575201890716805 1104 66-67 0.10513267514147273 1104 68-69 0.04742902572222718 1104 70-71 0.029438848859477673 1104 72-73 0.31374184142487493 1104 74-75 0.348691631640051 1104 76-77 0.2712991064618926 1104 78-79 0.5623353794555506 1104 80-81 0.553024446294625 1104 82-83 0.5747906469869939 1104 84-85 0.4074137617354232 1104 86-87 0.4129635588966849 1104 88-89 0.3016586930313636 1104 90-91 0.12298034524468804 1104 92-93 0.1486458101049628 1104 94-95 0.4009521347752809 1104 96-97 0.40417007485539713 1104 98-99 0.3645352049732189 1104 100-101 0.41417173568421717 1105 1 0.12115914407458916 1105 2 0.1364209517181436 1105 3 0.09242013170027263 1105 4 0.10121945804615251 1105 5 0.06285798608937654 1105 6 0.0573670180833048 1105 7 -0.008971394937809407 1105 8 0.03916517962045418 1105 9 -0.07552110489093167 1105 10-11 -0.06108059123216236 1105 12-13 0.10576026536863026 1105 14-15 0.29655715874861954 1105 16-17 0.2415354747535332 1105 18-19 0.23750343518412365 1105 20-21 0.2710616116707669 1105 22-23 -0.03367380220352345 1105 24-25 0.09983152993285671 1105 26-27 0.14603796299557104 1105 28-29 -0.055050222674879024 1105 30-31 0.1772176925800295 1105 32-33 0.11114391808328605 1105 34-35 0.1864303817944446 1105 36-37 0.08621666656142679 1105 38-39 0.2911476651524012 1105 40-41 0.5350089053718676 1105 42-43 0.41005128657010204 1105 44-45 0.2308872333432106 1105 46-47 0.20830920711280498 1105 48-49 0.3107500412606541 1105 50-51 0.024798826535224805 1105 52-53 0.11204592877787434 1105 54-55 0.009060111894612533 1105 56-57 -0.02696404712006739 1105 58-59 0.24280537004698743 1105 60-61 0.24667210095115166 1105 62-63 0.2480561516537385 1105 64-65 0.3939968421705231 1105 66-67 0.24488208634901198 1105 68-69 0.29039631574091374 1105 70-71 0.3176375599159762 1105 72-73 0.28930375423238885 1105 74-75 0.21286537021619267 1105 76-77 0.15090784747000896 1105 78-79 -0.005038158163170436 1105 80-81 0.36801081446911255 1105 82-83 0.3135115913589068 1105 84-85 0.046966375461970244 1105 86-87 0.21395650618696038 1105 88-89 0.2394434893859696 1105 90-91 0.1482256221051479 1105 92-93 0.24156302076466574 1105 94-95 0.37949880937784286 1105 96-97 0.41084358943441046 1105 98-99 0.7065204275623707 1105 100-101 1.357485261271087 1106 1 0.13822953577449937 1106 2 0.0860072439996955 1106 3 0.08100372636194919 1106 4 0.12068397304255285 1106 5 0.0854286078500266 1106 6 0.15433159332005175 1106 7 0.08705285725006462 1106 8 0.156863333053181 1106 9 0.01369885726666098 1106 10-11 0.09523448985134308 1106 12-13 -0.11813702083265554 1106 14-15 0.10037473263484031 1106 16-17 0.11676051545880028 1106 18-19 0.05428128831014334 1106 20-21 0.06008128903497578 1106 22-23 0.14318409599374604 1106 24-25 -0.0846212410654914 1106 26-27 0.01473553238383829 1106 28-29 0.18095824080627665 1106 30-31 0.06411673779930283 1106 32-33 0.19771904237919813 1106 34-35 0.005106666261958992 1106 36-37 0.19452134270360943 1106 38-39 0.2625948905970077 1106 40-41 0.38738421073302476 1106 42-43 0.348213428326531 1106 44-45 0.3190920023128925 1106 46-47 0.23383189247807223 1106 48-49 0.28701878891558863 1106 50-51 -0.15257254886078186 1106 52-53 -0.044954457907749656 1106 54-55 0.07610158823047897 1106 56-57 -0.13118155950375154 1106 58-59 0.1149716356851016 1106 60-61 0.029734008516072663 1106 62-63 0.09604739442919552 1106 64-65 -0.1045743142948723 1106 66-67 -0.13183691483169824 1106 68-69 0.13252502582881576 1106 70-71 0.10948279616712142 1106 72-73 -0.01925616884356174 1106 74-75 0.028927223851674455 1106 76-77 -0.09241149862968712 1106 78-79 -0.17505447469001822 1106 80-81 0.201609205306152 1106 82-83 0.06951152939741334 1106 84-85 -0.15357228793680022 1106 86-87 -0.02737003203581878 1106 88-89 -0.042026352004079115 1106 90-91 0.018876412860031166 1106 92-93 0.08940022038050444 1106 94-95 0.4269702066898624 1106 96-97 0.43476330799153473 1106 98-99 0.17137190930266044 1106 100-101 0.23176653662223146 1107 1 0.036048262132013065 1107 2 0.031955920621520306 1107 3 -0.001110671728007162 1107 4 0.01773952425467229 1107 5 0.11089845070450366 1107 6 0.0017657251105944738 1107 7 0.061285321962984085 1107 8 0.0038915206174507944 1107 9 0.10847330262657096 1107 10-11 0.06470086864842273 1107 12-13 0.11151855804380517 1107 14-15 -0.10244913568286762 1107 16-17 0.1937864778473113 1107 18-19 0.14750201973143362 1107 20-21 0.020910678194162813 1107 22-23 0.0036135549151978807 1107 24-25 -0.025816761846826353 1107 26-27 -0.09838067591666544 1107 28-29 0.07338362224351158 1107 30-31 -0.017986233712434796 1107 32-33 -0.0441347094475546 1107 34-35 -0.03567870050112987 1107 36-37 0.10536456403642802 1107 38-39 0.12635459567101037 1107 40-41 0.3214910432254001 1107 42-43 0.14472381147773916 1107 44-45 0.3017996803720564 1107 46-47 0.3786364077806681 1107 48-49 0.3824788289687433 1107 50-51 0.5150983536584803 1107 52-53 0.5566046686205581 1107 54-55 0.5896724540644129 1107 56-57 0.6009830553904862 1107 58-59 0.6084269126593398 1107 60-61 0.5094500279773442 1107 62-63 0.7606123725683496 1107 64-65 0.6765991952884178 1107 66-67 0.542416598841104 1107 68-69 0.5920746382272561 1107 70-71 0.6749778376335733 1107 72-73 0.6843386928046336 1107 74-75 0.6445151814410224 1107 76-77 0.8205793180098162 1107 78-79 0.751337011907804 1107 80-81 0.9646400149694294 1107 82-83 1.1183550394016812 1107 84-85 1.015179929721473 1107 86-87 0.7716373591560881 1107 88-89 0.6448577403948406 1107 90-91 0.6570586246847334 1107 92-93 0.8666754625938928 1107 94-95 0.6466236205304554 1107 96-97 0.6645478869221293 1107 98-99 0.7426500021466147 1107 100-101 0.7089548767012559 1108 1 0.043774958814744025 1108 2 -0.05854889702131061 1108 3 -0.06886207753720441 1108 4 -0.03992890219428347 1108 5 0.05664790780424056 1108 6 0.06400661362021509 1108 7 0.053591103771211124 1108 8 0.07867165397031783 1108 9 0.04420728468060986 1108 10-11 0.03190746369258335 1108 12-13 -0.08101807578349707 1108 14-15 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-0.17637804470911433 2316 82-83 -0.2884170443800862 2316 84-85 -0.45348154099314897 2316 86-87 -0.5146580216504262 2316 88-89 -0.436674552959623 2316 90-91 -0.16623090969015308 2316 92-93 -0.10167298946182157 2316 94-95 -0.21665542732512932 2316 96-97 -0.3289575672506011 2316 98-99 -0.48714984725750554 2316 100-101 -1.1620290032320426 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 3.0 4 10.0 5 29.0 6 89.0 7 209.0 8 442.0 9 959.0 10 1770.0 11 2957.0 12 4603.0 13 6478.0 14 8161.0 15 11742.0 16 16728.0 17 23291.0 18 19715.0 19 10049.0 20 7933.0 21 7455.0 22 7688.0 23 8071.0 24 8943.0 25 10347.0 26 12130.0 27 14756.0 28 18071.0 29 22222.0 30 27001.0 31 33693.0 32 42422.0 33 54778.0 34 75073.0 35 106243.0 36 150792.0 37 208484.0 38 139136.0 39 9003.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.83602348789664 20.119773336196193 15.773485562785314 19.270717613121853 2 15.835375858934128 22.5423890175903 39.866210684104516 21.756024439371057 3 18.61922069582197 28.54001340344943 31.757027943765248 21.083737956963354 4 12.243776103035534 18.58876782006113 37.92332998903381 31.244126087869528 5 12.08735412900209 39.57349620919742 32.945028462519915 15.394121199280574 6 28.059891215482196 38.65480888046022 17.551583049923657 15.733716854133922 7 25.633705281312878 36.84216911998029 20.79327955082522 16.730846047881613 8 27.286098802026366 33.230795650112555 19.738006264256036 19.745099283605047 9 28.51720430508943 15.706744714767293 20.7909463207762 34.985104659367074 10-11 24.339835889931273 28.316126539465188 26.219392688217003 21.12464488238654 12-13 26.79971702533146 26.60419198225995 27.676686508492036 18.919404483916548 14-15 23.48083391508536 26.10585771403186 28.743107638435205 21.67020073244758 16-17 19.70613434178647 29.90085638875719 28.928179445923192 21.464829823533144 18-19 19.68550383226871 26.192427302627898 34.83626524600813 19.28580361909526 20-21 21.4269781523836 24.90468675190183 35.17653833482506 18.49179676088951 22-23 20.733735519974317 25.341631543776995 35.104192721068884 18.820440215179808 24-25 20.128315274025628 25.57443438565609 35.45871450458199 18.838535835736288 26-27 19.98529988239906 25.782372925650073 35.251628679696104 18.980698512254765 28-29 19.597163530979252 25.665844535550363 35.49422369277652 19.242768240693856 30-31 19.720094832789293 25.839927890483906 35.38384266980722 19.05613460691958 32-33 19.523701972613377 26.049864112174653 35.00796101064704 19.418472904564936 34-35 19.87194833133836 25.8420307202673 34.96585211058923 19.320168837805106 36-37 20.270837903304848 25.669673902390077 34.57325411530797 19.48623407899711 38-39 19.998189301102894 25.97914242873523 34.60567598103246 19.416992289129414 40-41 19.92713126126761 26.107580094243072 34.51875317639844 19.446535468090875 42-43 20.039481144953204 26.38371846116871 33.95770477343843 19.61909562043966 44-45 20.112725903298198 26.76591548730527 33.35615290888923 19.765205700507305 46-47 20.644766290419224 27.052156967421965 32.21530848619358 20.087768255965237 48-49 20.819565106814682 27.473112584388478 31.294539172539114 20.41278313625773 50-51 21.007355126958892 27.800895905890215 30.734360030221307 20.45738893692959 52-53 21.07396769323742 28.10977672727782 30.563933370510455 20.25232220897431 54-55 20.726580382329676 28.161499639509735 30.442178061192347 20.669741916968242 56-57 20.88025878490185 28.51048420439602 29.945523388917454 20.663733621784672 58-59 20.947691785044142 27.842727700772723 30.382471127188765 20.827109386994366 60-61 20.962355182176353 27.86358624158928 30.728980737310373 20.44507783892399 62-63 21.0591474369444 27.876385356634547 30.894607900324317 20.169859306096736 64-65 20.941842009593984 27.88952403886315 30.698370963985923 20.470262987556943 66-67 20.956526567926975 28.2955060358638 30.189865236607073 20.558102159602154 68-69 21.125367838180136 28.187534240566404 30.21098255376061 20.47611536749286 70-71 20.962348934438584 28.33678899377981 30.147872400496347 20.552989671285257 72-73 21.0503791456808 28.312857724229858 29.753041939374025 20.883721190715317 74-75 20.960574788061837 28.64196240320098 29.63274940476246 20.76471340397472 76-77 20.88105574743299 28.73602160849444 29.72913315422699 20.653789489845582 78-79 20.778369811299623 28.667541882728827 29.53578857418343 21.018299731788122 80-81 20.78487205475821 29.041115323376953 29.290771404625776 20.883241217239057 82-83 20.690276323329844 28.882162650523696 29.192356728322817 21.23520429782365 84-85 20.629909722241667 28.756961224335516 29.10956059473378 21.503568458689035 86-87 20.731168966920396 28.692989856982333 28.99001004222213 21.585831133875143 88-89 20.72101080013589 28.491518178797687 29.035491493913025 21.751979527153402 90-91 20.397650328660934 28.709370033104086 29.196271660682672 21.69670797755231 92-93 20.287080893161296 28.68256375557059 29.10735201474603 21.923003336522086 94-95 20.15117605224991 28.598107639439796 29.072177745257434 22.178538563052864 96-97 20.526312596625115 28.459873249315077 28.73113520773029 22.28267894632952 98-99 20.28019345313092 28.517397529011433 28.720622245616784 22.48178677224086 100-101 20.20131221592765 28.75106162561714 28.76814096521601 22.279485193239196 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2119.0 1 1512.0 2 1020.5 3 1188.5 4 1035.0 5 975.5 6 1703.0 7 3640.0 8 5939.5 9 6674.5 10 5466.5 11 4275.0 12 4069.0 13 4058.5 14 4204.5 15 4370.5 16 4469.5 17 4599.5 18 4645.0 19 4748.5 20 4983.5 21 5360.0 22 5983.0 23 6824.5 24 7651.0 25 8811.5 26 10281.0 27 11540.5 28 12543.0 29 14043.0 30 16140.5 31 18015.5 32 20541.5 33 22828.5 34 25146.5 35 27161.0 36 29414.5 37 32127.5 38 32607.5 39 31839.0 40 31298.0 41 31332.5 42 31321.0 43 32168.5 44 33795.5 45 34469.5 46 33811.5 47 32964.5 48 35066.0 49 34725.0 50 31229.5 51 29155.5 52 27914.0 53 27766.0 54 26929.0 55 25397.0 56 23297.5 57 21058.5 58 20419.5 59 19424.5 60 16871.5 61 14430.5 62 12122.0 63 9004.5 64 6002.0 65 4374.0 66 3207.0 67 2508.0 68 1814.5 69 1021.0 70 708.5 71 561.0 72 449.5 73 402.5 74 321.5 75 237.5 76 173.0 77 88.0 78 45.5 79 32.0 80 20.5 81 12.0 82 9.0 83 8.0 84 6.0 85 3.0 86 1.0 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.026692151760748725 2 0.008399628176459388 3 0.010266212215672586 4 9.332920196065987E-5 5 0.006253056531364211 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 1.8665840392131973E-4 14-15 0.0 16-17 0.0 18-19 0.004479801694111675 20-21 8.399628176459389E-4 22-23 0.0 24-25 0.004899783102934644 26-27 0.005226435309796953 28-29 0.0068596963441085014 30-31 0.030565313642116107 32-33 3.7331680784263946E-4 34-35 0.0031265282656821056 36-37 0.01595929353527284 38-39 0.006206391930383881 40-41 0.009099597191164337 42-43 0.0073263423539118 44-45 0.0025665530539181465 46-47 0.0016799256352918778 48-49 0.0016332610343115477 50-51 0.004339807891170684 52-53 0.0026598822558788066 54-55 0.0017265902362722076 56-57 6.533044137246191E-4 58-59 8.866274186262688E-4 60-61 4.666460098032993E-5 62-63 9.332920196065987E-5 64-65 0.007046354748029821 66-67 5.133106107836293E-4 68-69 0.0012132796254885785 70-71 0.004246478689210025 72-73 0.011199504235279184 74-75 0.005133106107836293 76-77 0.004246478689210025 78-79 0.011339498038220175 80-81 1.8665840392131973E-4 82-83 0.004666460098032994 84-85 4.6664600980329937E-4 86-87 0.0 88-89 7.466336156852789E-4 90-91 6.533044137246191E-4 92-93 9.332920196065987E-5 94-95 9.332920196065987E-4 96-97 2.3332300490164969E-4 98-99 1.8665840392131973E-4 100-101 5.599752117639593E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1071476.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.775452384736184 #Duplication Level Percentage of deduplicated Percentage of total 1 74.86497505743979 36.51573026198617 2 13.423984600285296 13.095218433692946 3 4.72677661401851 6.9165200301103305 4 2.1878737959133847 4.268581366255413 5 1.1987852928761054 2.923564748610025 6 0.7446931314802072 2.1793646625451757 7 0.48633475746181637 1.6604838463934735 8 0.35614358187550876 1.3896851455918617 9 0.26372298233280506 1.157688699078092 >10 1.5445163428887105 14.112676699705911 >50 0.1343566848614035 4.5395800412978335 >100 0.06323802945995552 5.502682726464361 >500 0.0024911949327059784 0.8109334389304375 >1k 0.0015330430355113714 1.3524904173955083 >5k 1.9163037943892142E-4 0.8523400218541562 >10k+ 3.8326075887784285E-4 2.722459460088388 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16323 1.5234125636038511 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12804 1.194987101904289 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9119 0.8510689926792573 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2660 0.24825567721535527 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2542 0.23724283138399743 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2307 0.21531046892324232 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2029 0.1893649507781789 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1326 0.12375452179983498 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1267 0.11824809888415606 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1214 0.11330165118024109 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1125 0.10499535220574235 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 9.332920196065988E-5 0.0 0.0 0.0 0.0 16-17 9.332920196065988E-5 0.0 0.0 9.332920196065988E-5 0.0 18-19 9.332920196065988E-5 0.0 0.0 9.332920196065988E-5 0.0 20-21 9.332920196065988E-5 0.0 0.0 1.3999380294098983E-4 0.0 22-23 9.332920196065988E-5 0.0 0.0 7.932982166656089E-4 0.0 24-25 9.332920196065988E-5 0.0 0.0 0.001493267231370558 0.0 26-27 9.332920196065988E-5 0.0 0.0 0.003079863664701776 0.0 28-29 9.332920196065988E-5 0.0 0.0 0.006159727329403552 0.0 30-31 9.332920196065988E-5 0.0 0.0 0.010966181230377535 0.0 32-33 9.332920196065988E-5 0.0 0.0 0.01591262893429251 0.0 34-35 9.332920196065988E-5 0.0 0.0 0.02090574123918781 0.0 36-37 9.332920196065988E-5 0.0 0.0 0.02809208979015862 0.0 38-39 9.332920196065988E-5 0.0 0.0 0.03933825862641814 0.0 40-41 9.332920196065988E-5 0.0 0.0 0.06276388831854376 0.0 42-43 9.332920196065988E-5 0.0 0.0 0.08301632514400695 0.0 44-45 9.332920196065988E-5 0.0 0.0 0.10774856366358182 0.0 46-47 1.8665840392131976E-4 0.0 0.0 0.15352653722528548 0.0 48-49 2.799876058819796E-4 0.0 0.0 0.18880497556641493 0.0 50-51 2.799876058819796E-4 0.0 0.0 0.23985604903889587 0.0 52-53 2.799876058819796E-4 0.0 0.0 0.30014671350548217 0.0 54-55 2.799876058819796E-4 0.0 0.0 0.36767039112401956 0.0 56-57 2.799876058819796E-4 0.0 0.0 0.49445811198757605 0.0 58-59 2.799876058819796E-4 0.0 0.0 0.5992668057893971 0.0 60-61 2.799876058819796E-4 0.0 0.0 0.6985690766755392 0.0 62-63 2.799876058819796E-4 0.0 0.0 0.8103774606244096 0.0 64-65 2.799876058819796E-4 0.0 0.0 0.9240057640115131 0.0 66-67 2.799876058819796E-4 0.0 0.0 1.07916556227111 0.0 68-69 2.799876058819796E-4 0.0 0.0 1.271890364319873 0.0 70-71 2.799876058819796E-4 0.0 0.0 1.405164464719695 0.0 72-73 2.799876058819796E-4 0.0 0.0 1.5669973009194793 0.0 74-75 4.1998140882296944E-4 0.0 0.0 1.793693932481922 0.0 76-77 5.599752117639592E-4 0.0 0.0 2.038216441618851 0.0 78-79 5.599752117639592E-4 0.0 0.0 2.3012181327439905 0.0 80-81 5.599752117639592E-4 0.0 0.0 2.575839309513232 0.0 82-83 5.599752117639592E-4 0.0 0.0 2.891478670544184 0.0 84-85 5.599752117639592E-4 0.0 0.0 3.2752016844054372 0.0 86-87 5.599752117639592E-4 0.0 0.0 3.6216396820834067 0.0 88-89 5.599752117639592E-4 0.0 0.0 4.037001295409324 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6795 0.0 48.952328 1 GTATCAA 11825 0.0 39.58219 1 ATAGCGC 55 0.0025816492 34.53972 6 ATCAACG 15640 0.0 29.824785 3 TCAACGC 15785 0.0 29.456413 4 TATCAAC 15825 0.0 29.418835 2 CAACGCA 16100 0.0 28.791594 5 GTAAGGT 400 0.0 28.4966 4 AACGCAG 16495 0.0 28.129612 6 ACGCAGA 18285 0.0 25.271986 7 CGCAGAG 18335 0.0 25.125362 8 CGTCACT 190 1.1857992E-8 25.009855 1 TAAGGTG 450 0.0 24.27488 5 GAGTACT 11395 0.0 24.154106 12-13 GCAGAGT 18950 0.0 24.009207 9 AGTACTT 11625 0.0 23.512796 12-13 GTACATG 9125 0.0 23.433891 1 TACATGG 9450 0.0 22.521086 2 GTACTTT 12305 0.0 22.292707 14-15 ACATGGG 9175 0.0 22.210266 3 >>END_MODULE