FastQCFastQC Report
Thu 26 May 2016
SRR936965_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936965_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1126485
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165491.4690830326191648No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114341.0150157347856386No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80410.7138133219705545No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24170.21456122362925384No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20920.1857104178040542No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15990.14194596465998216No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15250.1353768581028598No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11570.10270886873771067No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56400.056.5363921
GTATCAA110600.044.9429131
ATCAACG142650.034.6818473
TCAACGC143900.034.3475534
TATCAAC144700.034.3218732
CAACGCA147050.033.579465
AACGCAG150050.033.0001836
ACGCAGA168400.029.2913887
CGCAGAG168650.029.0776088
GCAGAGT172350.027.8142999
GTACATG101150.026.6849941
TACATGG102100.026.1132142
GAGTACT103300.025.7547112-13
ACATGGG102950.025.1128373
GTACTTT110550.023.82773614-15
AGTACTT105300.023.61876712-13
CATGGGA73650.023.2302024
CAGAGTA171300.022.52095810-11
TAAGGTA3007.2759576E-1222.178394
AGAGTAC166400.022.08488810-11
Produced by FastQC (version 0.11.5)