##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936965_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1126485 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.91190029161507 33.0 31.0 34.0 30.0 34.0 2 32.00432673315668 33.0 31.0 34.0 30.0 34.0 3 31.973501644495933 33.0 31.0 34.0 30.0 34.0 4 35.60613590061119 37.0 35.0 37.0 33.0 37.0 5 35.6183775194521 37.0 35.0 37.0 33.0 37.0 6 35.7495102020888 37.0 35.0 37.0 35.0 37.0 7 35.70230140658775 37.0 35.0 37.0 33.0 37.0 8 35.66707235338242 37.0 35.0 37.0 33.0 37.0 9 37.272036467418566 39.0 37.0 39.0 34.0 39.0 10-11 37.156002077258016 39.0 37.0 39.0 33.5 39.0 12-13 37.060070040879374 39.0 37.0 39.0 33.0 39.0 14-15 38.13959972835857 40.0 38.0 41.0 33.0 41.0 16-17 38.10669338695145 40.0 38.0 41.0 33.0 41.0 18-19 38.17915462700347 40.0 38.0 41.0 33.5 41.0 20-21 38.14043684558605 40.0 38.0 41.0 33.5 41.0 22-23 38.006139007621044 40.0 38.0 41.0 33.0 41.0 24-25 38.07450787183141 40.0 38.0 41.0 33.0 41.0 26-27 37.99289160530323 40.0 38.0 41.0 33.0 41.0 28-29 37.87262857472581 40.0 37.5 41.0 33.0 41.0 30-31 37.5706112376108 39.5 37.0 41.0 33.0 41.0 32-33 37.227216518639835 39.0 35.5 41.0 32.5 41.0 34-35 36.97363213890998 39.0 35.0 41.0 31.5 41.0 36-37 36.72707137689361 39.0 35.0 41.0 31.0 41.0 38-39 36.5108985028651 39.0 35.0 41.0 31.0 41.0 40-41 36.23680341948628 39.0 35.0 41.0 30.0 41.0 42-43 35.873656994988835 39.0 35.0 40.0 29.5 41.0 44-45 35.39844871436371 38.5 35.0 40.0 27.5 41.0 46-47 34.75193145048536 38.0 34.5 40.0 24.0 41.0 48-49 34.236840259746025 38.0 34.0 40.0 21.0 41.0 50-51 33.42437538005389 37.5 33.5 39.5 16.5 40.5 52-53 33.56848071656525 38.0 33.5 39.5 17.5 40.5 54-55 33.930098936071055 38.0 34.0 40.0 15.0 41.0 56-57 33.82508022743312 38.0 34.0 40.0 13.5 41.0 58-59 33.58440724909786 38.0 34.0 40.0 9.5 41.0 60-61 33.317374399126486 38.0 34.0 40.0 8.0 41.0 62-63 32.989623918649606 37.0 33.0 40.0 6.5 41.0 64-65 32.649676205186935 36.5 33.0 40.0 2.0 41.0 66-67 32.28281157760645 36.0 32.5 39.0 2.0 41.0 68-69 31.894118430338622 35.5 32.0 39.0 2.0 41.0 70-71 31.503404838945926 35.0 31.5 39.0 2.0 40.0 72-73 31.1125270198893 35.0 31.0 38.0 2.0 40.0 74-75 30.69147924739344 35.0 31.0 37.0 2.0 39.5 76-77 30.270103019569724 35.0 31.0 37.0 2.0 39.0 78-79 29.85832789606608 35.0 30.0 36.0 2.0 39.0 80-81 29.43687754386432 34.5 30.0 36.0 2.0 37.5 82-83 29.02939542026747 34.0 29.0 35.0 2.0 37.0 84-85 28.65053551534197 34.0 29.0 35.0 2.0 37.0 86-87 28.303554863136213 34.0 29.0 35.0 2.0 36.0 88-89 27.94105824755767 34.0 28.0 35.0 2.0 36.0 90-91 27.554576403591703 34.0 27.0 35.0 2.0 35.5 92-93 27.212659289737545 34.0 26.0 35.0 2.0 35.0 94-95 26.84217588338948 34.0 25.5 35.0 2.0 35.0 96-97 26.356295467760333 33.5 25.0 35.0 2.0 35.0 98-99 25.744134187317187 33.0 22.0 35.0 2.0 35.0 100-101 23.443772442597993 31.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.31541909395608414 1101 2 0.4737796010910209 1101 3 0.3456851355249384 1101 4 0.2533508643256326 1101 5 0.18398784669646773 1101 6 0.25288094053082943 1101 7 0.06353576261158622 1101 8 0.2378468378573828 1101 9 0.4082823876228403 1101 10-11 0.2513869399111641 1101 12-13 0.3891141450393434 1101 14-15 0.45671498982274983 1101 16-17 0.4543514242460205 1101 18-19 0.41340313277154195 1101 20-21 0.40124353894770337 1101 22-23 0.46183345671604314 1101 24-25 0.484226071390637 1101 26-27 0.5500966277129962 1101 28-29 0.530747819650081 1101 30-31 0.48640809958337883 1101 32-33 0.6244341415829879 1101 34-35 0.5863215139372144 1101 36-37 0.5539059106903466 1101 38-39 0.5605658420957624 1101 40-41 0.6045213114320447 1101 42-43 0.7797994474542165 1101 44-45 0.7686632808980036 1101 46-47 0.7776774959193844 1101 48-49 0.7968422802962891 1101 50-51 0.8898574618005242 1101 52-53 0.721229398905642 1101 54-55 0.9039357092407201 1101 56-57 0.7335473091232529 1101 58-59 0.7817212661854 1101 60-61 1.0612149656697412 1101 62-63 1.0492108105001563 1101 64-65 0.9108492433026285 1101 66-67 0.8596310960835822 1101 68-69 0.9136012823424302 1101 70-71 0.8418657289304292 1101 72-73 0.8952829145750236 1101 74-75 0.862234224567306 1101 76-77 0.8834445710547172 1101 78-79 0.7244334133300647 1101 80-81 0.6057731897057756 1101 82-83 0.6491192412921016 1101 84-85 0.7808743538881728 1101 86-87 0.9485307064806534 1101 88-89 0.9148799790833024 1101 90-91 0.8483500535481809 1101 92-93 0.6204808694498887 1101 94-95 0.8532188281446338 1101 96-97 0.7700049559339703 1101 98-99 0.7865034157019686 1101 100-101 1.0150328196489795 1102 1 0.2608775819470033 1102 2 0.2968819365799895 1102 3 0.24508946466178827 1102 4 0.11651493529592472 1102 5 0.2603332082444467 1102 6 0.30353209679557125 1102 7 0.17137132173861147 1102 8 0.22475738518579647 1102 9 0.28370937010986097 1102 10-11 0.39494106687706676 1102 12-13 0.26079107969412973 1102 14-15 0.4568961145819941 1102 16-17 0.5317733301376748 1102 18-19 0.46927501889254586 1102 20-21 0.6147513643026272 1102 22-23 0.5659240701167221 1102 24-25 0.13147667407689312 1102 26-27 0.37512755922998053 1102 28-29 0.3583935804378413 1102 30-31 0.5293372185772398 1102 32-33 0.6888456774444123 1102 34-35 0.5432878017725145 1102 36-37 0.2811741156419387 1102 38-39 0.42256025528980956 1102 40-41 0.33806000452761964 1102 42-43 0.5475617913616659 1102 44-45 0.4091497666950161 1102 46-47 0.7151524637123501 1102 48-49 0.8101166843065499 1102 50-51 0.6696633266712482 1102 52-53 0.8274769275731018 1102 54-55 0.884580870531039 1102 56-57 0.9262576753269016 1102 58-59 0.8002635945314225 1102 60-61 0.757716196552785 1102 62-63 0.9768042747025021 1102 64-65 1.1272104213788836 1102 66-67 0.8844337083618043 1102 68-69 1.0104966509058038 1102 70-71 0.9448632161432755 1102 72-73 0.7808337506826248 1102 74-75 0.8430936743051909 1102 76-77 0.8406328004964188 1102 78-79 1.0064446634735589 1102 80-81 0.8760776578750118 1102 82-83 1.000334180258161 1102 84-85 1.0439925059054183 1102 86-87 1.0689034153162282 1102 88-89 1.1752736065530414 1102 90-91 0.7937320099506842 1102 92-93 0.9867317144861758 1102 94-95 1.112345857826135 1102 96-97 1.0146509335612386 1102 98-99 1.0650082435696682 1102 100-101 1.0812249904674829 1103 1 0.023195599487060292 1103 2 0.10520810892687749 1103 3 0.1531384134289766 1103 4 0.2654514976737161 1103 5 0.20309413590977243 1103 6 0.10844839420441588 1103 7 0.09045441657996633 1103 8 0.04763368568320203 1103 9 0.21776801796617207 1103 10-11 0.19594759022437813 1103 12-13 0.2733645265073008 1103 14-15 0.2538942290631283 1103 16-17 0.2521192493717166 1103 18-19 0.2605189382810096 1103 20-21 0.29007771847629726 1103 22-23 0.27503583381164276 1103 24-25 0.014657198186370124 1103 26-27 0.09233310569420183 1103 28-29 0.1362906664767891 1103 30-31 0.22364728555425728 1103 32-33 0.39322919746154383 1103 34-35 0.45540344360861695 1103 36-37 0.5501303053495477 1103 38-39 0.4567162925618078 1103 40-41 0.35158592480778594 1103 42-43 0.42990591225406405 1103 44-45 0.6051279076282157 1103 46-47 0.6862641726101515 1103 48-49 0.7494757075230893 1103 50-51 0.861213113585201 1103 52-53 0.7703384826653732 1103 54-55 0.6695368211239696 1103 56-57 0.6572962549857166 1103 58-59 0.7792568719218806 1103 60-61 0.724241137957236 1103 62-63 0.8212220678234701 1103 64-65 0.6011195229303965 1103 66-67 0.2259696714584365 1103 68-69 0.47532836149733626 1103 70-71 0.6807002794165058 1103 72-73 0.6735542032071535 1103 74-75 0.5268510625751155 1103 76-77 0.46409518103248715 1103 78-79 0.40999327474034075 1103 80-81 0.6159510267268367 1103 82-83 0.47815853558256904 1103 84-85 0.49500264088067425 1103 86-87 0.6039665938975247 1103 88-89 0.4391002882640045 1103 90-91 0.4084288449192144 1103 92-93 0.5602114059286087 1103 94-95 0.5350537817378509 1103 96-97 0.3485623764439616 1103 98-99 0.46638639097888657 1103 100-101 0.6621144970692896 1104 1 0.3095417465606012 1104 2 0.3800544784663522 1104 3 0.27935224163368844 1104 4 0.3120557548334233 1104 5 0.3645736509389934 1104 6 0.2841895381009394 1104 7 0.29977576403405237 1104 8 0.40736581388112114 1104 9 0.4171962036312351 1104 10-11 0.4619531089668243 1104 12-13 0.4926799762580245 1104 14-15 0.7576436604977346 1104 16-17 0.4514973367995836 1104 18-19 0.48419293466698576 1104 20-21 0.6181866985109039 1104 22-23 0.5440479908185267 1104 24-25 0.49562210015503183 1104 26-27 0.5222359994378181 1104 28-29 0.5421156529973885 1104 30-31 0.48637558594924 1104 32-33 0.6170405919831836 1104 34-35 0.6234287149451347 1104 36-37 0.46847811508934 1104 38-39 0.4934658296491392 1104 40-41 0.6435038886963795 1104 42-43 0.882163036248194 1104 44-45 0.7489569704588419 1104 46-47 0.7517753060753236 1104 48-49 1.0263976817507654 1104 50-51 0.8418429525912927 1104 52-53 0.9654735966190202 1104 54-55 0.975884317377762 1104 56-57 0.9667610024434836 1104 58-59 1.097172100744345 1104 60-61 1.0980084609108118 1104 62-63 1.0560610267920225 1104 64-65 0.902090883107455 1104 66-67 0.7829286390180954 1104 68-69 0.8840992235689562 1104 70-71 0.8410610164855825 1104 72-73 0.8628327836314398 1104 74-75 0.9187792445200138 1104 76-77 0.9002975560857429 1104 78-79 1.0236927121024202 1104 80-81 1.0177145838802453 1104 82-83 1.0664482462478908 1104 84-85 1.0915238391313018 1104 86-87 1.1198854327691947 1104 88-89 1.0754079192175716 1104 90-91 1.0425849797955742 1104 92-93 0.8431560811060272 1104 94-95 1.0483600719435486 1104 96-97 0.8946391140467576 1104 98-99 0.6994982008198676 1104 100-101 1.0124169961776914 1105 1 0.2562502041447523 1105 2 0.35541347320999606 1105 3 0.30353228779051733 1105 4 0.2890468900010532 1105 5 0.30365343849167203 1105 6 0.28277721627838304 1105 7 0.2664212103368868 1105 8 0.35813558511747345 1105 9 0.3872140632580212 1105 10-11 0.3962121172143327 1105 12-13 0.41976924291677165 1105 14-15 0.35534566770047604 1105 16-17 0.312814898021351 1105 18-19 0.27503604307462126 1105 20-21 0.2828336231319142 1105 22-23 0.23536073938361568 1105 24-25 0.25912546038442485 1105 26-27 0.1909274847922191 1105 28-29 0.3256577886188623 1105 30-31 0.01913312653953625 1105 32-33 0.11462944083428539 1105 34-35 -0.1384272749340738 1105 36-37 0.13839814782352278 1105 38-39 0.12457848744386979 1105 40-41 0.26648528889517564 1105 42-43 0.32417864590721734 1105 44-45 0.21702895318200888 1105 46-47 0.288397440437798 1105 48-49 0.06042565201789074 1105 50-51 0.30366761958895694 1105 52-53 0.31641323229887774 1105 54-55 0.24625434366461008 1105 56-57 0.4220728453356202 1105 58-59 0.4497606297669918 1105 60-61 0.33414389759848717 1105 62-63 0.1755271061927317 1105 64-65 0.29105380635979117 1105 66-67 0.45741917260363607 1105 68-69 0.4860288044515393 1105 70-71 0.41496804083213945 1105 72-73 0.3096610499683301 1105 74-75 0.2886710003578976 1105 76-77 0.23442056109232823 1105 78-79 0.34289673523972297 1105 80-81 0.5400495244784587 1105 82-83 0.5509368202495928 1105 84-85 0.6007143380941429 1105 86-87 0.5924553203621876 1105 88-89 0.5587456184630817 1105 90-91 0.4967989988411041 1105 92-93 0.49833151462587466 1105 94-95 0.5604747167359783 1105 96-97 0.6024885164752192 1105 98-99 0.24709984280897856 1105 100-101 0.5574696561858374 1106 1 0.03413076903738954 1106 2 0.1935427758803847 1106 3 0.20498760549246953 1106 4 0.17989911838698447 1106 5 0.30166440221591984 1106 6 0.23433491628292558 1106 7 0.13858959252754488 1106 8 0.20569630959766982 1106 9 0.2888166974242452 1106 10-11 0.29689283544281864 1106 12-13 0.29010752164796827 1106 14-15 0.34644437395566996 1106 16-17 0.40017224129827866 1106 18-19 0.23564472936025282 1106 20-21 0.22870591084571856 1106 22-23 0.24163832161946885 1106 24-25 0.30939654379933756 1106 26-27 0.18671523714426286 1106 28-29 0.2755004926521636 1106 30-31 0.21123326794221953 1106 32-33 0.22024240932968553 1106 34-35 0.3345132250548417 1106 36-37 0.07493564065570979 1106 38-39 0.1699918236496032 1106 40-41 0.28726931873441686 1106 42-43 0.13055306627764907 1106 44-45 0.27250601035875377 1106 46-47 0.4249009290395165 1106 48-49 0.2131772490022854 1106 50-51 0.16313827298763783 1106 52-53 0.33551949129495284 1106 54-55 0.12895426900315243 1106 56-57 0.42294565578527 1106 58-59 0.16904567498100675 1106 60-61 0.21985747426271018 1106 62-63 0.2627659098774444 1106 64-65 0.30288954903013376 1106 66-67 0.3589465644897203 1106 68-69 0.39628905297810846 1106 70-71 0.31265464432697243 1106 72-73 0.35615285885610604 1106 74-75 0.4000469994961726 1106 76-77 0.4380919091488593 1106 78-79 0.5013844340509443 1106 80-81 0.40245726688268135 1106 82-83 0.5248282770563009 1106 84-85 0.6614897718328869 1106 86-87 0.5879038224335957 1106 88-89 0.6829162373295183 1106 90-91 0.49741123339425997 1106 92-93 0.3228086995824846 1106 94-95 0.4817984845314385 1106 96-97 0.6813862513921798 1106 98-99 0.6972551323148721 1106 100-101 0.513073542806012 1107 1 0.16138516892358368 1107 2 0.23363853091819564 1107 3 0.31381116105394824 1107 4 0.0976515867242469 1107 5 0.2900973359031127 1107 6 0.2174338631553283 1107 7 0.1602956236723827 1107 8 0.145262611292722 1107 9 0.3498410851743614 1107 10-11 0.2300023958320594 1107 12-13 0.1454961319551984 1107 14-15 0.313933115848279 1107 16-17 0.31477399547367213 1107 18-19 0.14584687517996997 1107 20-21 0.2814228600208608 1107 22-23 0.24927671965635767 1107 24-25 0.1716592122264018 1107 26-27 0.0811110224111431 1107 28-29 0.2486402376610357 1107 30-31 0.14712234724463968 1107 32-33 0.10362069729718115 1107 34-35 0.2474584926488248 1107 36-37 0.290859583416065 1107 38-39 0.25733023146649003 1107 40-41 0.21734069046756588 1107 42-43 0.461009358592797 1107 44-45 0.5899601888614399 1107 46-47 0.5789614049696112 1107 48-49 0.49985334002735726 1107 50-51 0.27804709676546935 1107 52-53 0.3154177126695785 1107 54-55 0.4389656493604761 1107 56-57 0.526124876408609 1107 58-59 0.3246323301784102 1107 60-61 0.21284116167630174 1107 62-63 0.40478782373347144 1107 64-65 0.460875081306348 1107 66-67 0.2856696772841687 1107 68-69 0.41436203841036345 1107 70-71 0.4702731489658021 1107 72-73 0.5412556488218279 1107 74-75 0.5812930819415278 1107 76-77 0.4514949471708114 1107 78-79 0.5353610243002898 1107 80-81 0.47786574836070983 1107 82-83 0.41975501600601817 1107 84-85 0.32207478221301855 1107 86-87 0.3084090814034042 1107 88-89 0.4414659530428544 1107 90-91 0.546983303063385 1107 92-93 0.4226496635343153 1107 94-95 0.42902513368302664 1107 96-97 0.3567862865112481 1107 98-99 0.10963082636108012 1107 100-101 0.12321600843155522 1108 1 -0.28296305770009056 1108 2 -0.10207312269765012 1108 3 -0.178141855988887 1108 4 -0.028037036030241325 1108 5 0.10611760306110085 1108 6 -0.13757074301694416 1108 7 -0.23414012022443842 1108 8 -0.03463605291732108 1108 9 -0.0991662847012762 1108 10-11 -0.22556761798645653 1108 12-13 -0.06443362391767948 1108 14-15 -0.2681672987072261 1108 16-17 0.021687860052210794 1108 18-19 -0.1580326734151427 1108 20-21 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-1.082451863066403 2316 62-63 -0.9976090186323141 2316 64-65 -1.1621780374847788 2316 66-67 -1.0068201135131076 2316 68-69 -0.7911901178969991 2316 70-71 -0.8693951035074399 2316 72-73 -1.4328015467754938 2316 74-75 -1.4763846166223082 2316 76-77 -1.80562227647604 2316 78-79 -1.9756078530998984 2316 80-81 -2.2041111417167727 2316 82-83 -1.7225748478025302 2316 84-85 -1.5476440178447284 2316 86-87 -1.7273312920402901 2316 88-89 -1.7543479300978646 2316 90-91 -1.5305958956001398 2316 92-93 -2.1456222312524567 2316 94-95 -2.292648195892042 2316 96-97 -2.12550272329921 2316 98-99 -1.8801324713832734 2316 100-101 -2.048396134676029 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1663.0 3 529.0 4 535.0 5 595.0 6 791.0 7 917.0 8 1181.0 9 1516.0 10 2116.0 11 3227.0 12 5076.0 13 7085.0 14 8071.0 15 11269.0 16 15630.0 17 22087.0 18 18390.0 19 8414.0 20 7560.0 21 7477.0 22 7603.0 23 8386.0 24 9076.0 25 10051.0 26 11653.0 27 13529.0 28 16145.0 29 19886.0 30 24082.0 31 30002.0 32 38302.0 33 51019.0 34 70648.0 35 104930.0 36 151382.0 37 212414.0 38 189504.0 39 33731.0 40 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.622121452399576 19.21349222486923 15.228299291700814 19.93608703103038 2 15.197791826954235 21.491156032568597 41.025213053363885 22.28583908711328 3 18.079258061022102 28.652652026997032 31.28248335768938 21.985606554291486 4 11.597623258820438 17.730197967055215 38.78648397155866 31.885694802565688 5 11.973392855080947 38.98351753277264 33.36862477939145 15.67446483275496 6 27.670900510299234 38.22146596021691 18.485691905726966 15.621941623756888 7 25.6422231357483 36.47635629766231 21.2600207940633 16.62139977252609 8 26.77402709056626 32.809853569547585 20.260727382858747 20.155391957027412 9 28.43633121761152 15.653381543849429 20.986758683398275 34.92352855514078 10-11 23.823646148641508 27.947510817225517 26.87320249366683 21.355640540466148 12-13 26.39822817094527 26.220796612637958 27.965185050991593 19.41579016542518 14-15 23.320549229602 25.764105095720957 28.740082848805592 22.17526282587145 16-17 19.69197207833834 29.72092544220248 28.76100733911394 21.826095140345238 18-19 19.64288964525582 26.250820725203667 34.38673237499562 19.7195572545449 20-21 21.437142229315057 25.01921057946921 34.47556560784671 19.068081583369032 22-23 20.699975052890533 25.489775680208243 34.36921544448025 19.44103382242097 24-25 19.919225150903557 25.755893772062944 34.77730545436629 19.547575622667203 26-27 19.831582087087828 25.920619343292646 34.518037769749846 19.729760799869684 28-29 19.564078777414302 25.754284420732116 34.89140807748132 19.790228724372263 30-31 19.653435243256677 25.77721851600332 34.89451701531756 19.674829225422442 32-33 19.440745613334258 26.14289442707473 34.35434387766516 20.062016081925854 34-35 19.822367807827 26.057692734479375 34.23987003821622 19.8800694194774 36-37 20.16258538728878 25.820805425726927 33.86986954997182 20.146739637012477 38-39 19.84461392944155 26.030451373276776 33.95404020206226 20.170894495219418 40-41 19.82875937096366 26.10451981162643 33.99423871600598 20.072482101403924 42-43 19.885262564525938 26.395114005068866 33.37616568351998 20.34345774688522 44-45 19.978783561254698 26.672747528817514 32.904033342654365 20.444435567273423 46-47 20.33978274889712 26.945954427246892 31.963067401282487 20.751195422573502 48-49 20.4691706976707 27.29904827173569 31.126600898198543 21.10518013239507 50-51 20.707316174626612 27.692505038910912 30.613320124085263 20.986858662377212 52-53 20.708552933521176 28.02182730437052 30.395018460175464 20.874601301932838 54-55 20.44541897569171 27.998233415522417 30.29563658072692 21.260711028058953 56-57 20.523275116690723 28.472270307026655 29.787337924618413 21.21711665166421 58-59 20.703895518227675 27.801307442515316 30.18192164222166 21.312875397035352 60-61 20.686864902749917 27.76613107621589 30.546011079926654 21.00099294110754 62-63 20.8432759502301 27.735684407135956 30.67288435316175 20.74815528947219 64-65 20.678615196758372 27.86957137359567 30.585754353975535 20.866059075670425 66-67 20.615684147258502 28.21893813150706 30.11529040557613 21.05008731565831 68-69 20.712304248175826 28.07484126550777 30.24924744557659 20.963607040739817 70-71 20.59394028187523 28.23143159675282 30.105020611210637 21.069607510161315 72-73 20.581508498533267 28.39448571976504 29.62083330373822 21.403172477963476 74-75 20.45930566542986 28.677104503559043 29.563823430440024 21.29976640057108 76-77 20.527063566414625 28.62011552686377 29.659457707488823 21.19336319923278 78-79 20.410614025391016 28.580941688209123 29.5858566082834 21.422587678116464 80-81 20.20530304779599 28.954195137807126 29.38816725488768 21.452334559509197 82-83 20.408677957311752 28.78750406826425 29.153547948240575 21.650270026183417 84-85 20.339055285269648 28.547918739314902 29.162554665750374 21.95047130966508 86-87 20.412647735666802 28.60141201204614 29.07014425535542 21.915795996931646 88-89 20.49547249984241 28.431049621827885 28.987454043587235 22.08602383474247 90-91 20.13257569871726 28.538315406919313 29.337046033006743 21.992062861356683 92-93 20.117427621153844 28.54914639869537 29.196134940533618 22.13729103961717 94-95 19.95617675965492 28.517467592021255 29.19892075504531 22.327434893278514 96-97 20.28320230994209 28.378609950437824 28.90446450293093 22.433723236689154 98-99 20.070433322100588 28.410637278602767 28.919973556425465 22.59895584287118 100-101 20.181716619051382 28.334631393761672 29.03078845404111 22.452863533145834 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1307.0 1 980.5 2 817.5 3 1055.5 4 955.5 5 938.5 6 1644.5 7 3451.0 8 5969.5 9 6863.0 10 5391.0 11 4091.0 12 3961.0 13 4142.5 14 4286.0 15 4277.5 16 4352.0 17 4477.0 18 4510.5 19 4595.0 20 4890.5 21 5369.0 22 6007.0 23 6828.5 24 7740.5 25 9062.5 26 10616.5 27 11948.5 28 13133.5 29 14580.5 30 16761.0 31 18881.5 32 21770.5 33 24593.0 34 26812.5 35 28579.0 36 31145.5 37 34196.0 38 34697.0 39 33741.0 40 33076.0 41 33481.0 42 33528.5 43 34322.5 44 35656.0 45 35841.5 46 35184.0 47 34699.5 48 37331.0 49 37054.5 50 33449.0 51 31224.5 52 29643.0 53 29511.0 54 28999.5 55 27330.0 56 24936.5 57 22352.5 58 21336.0 59 20126.0 60 17669.0 61 15287.5 62 12691.5 63 9397.5 64 6481.5 65 4845.5 66 3428.5 67 2570.5 68 1829.5 69 1116.5 70 784.5 71 583.0 72 459.0 73 403.5 74 344.5 75 276.0 76 193.0 77 89.0 78 50.0 79 35.0 80 21.0 81 17.0 82 12.5 83 8.5 84 8.0 85 7.5 86 4.5 87 4.0 88 4.5 89 2.5 90 1.5 91 0.5 92 0.5 93 2.5 94 2.5 95 1.5 96 1.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023613274921548 2 0.01100769206869155 3 0.012339267722162301 4 0.02103889532483788 5 0.05521600376392051 6 0.04252164920083268 7 0.017931885466739458 8 0.04953461430911197 9 0.027785545302423026 10-11 0.0241459051829363 12-13 0.0370178031664869 14-15 0.019219075265094518 16-17 0.01695539665419424 18-19 0.017266097640004082 20-21 0.013537685810285978 22-23 0.009054714443601114 24-25 0.001819820059743361 26-27 1.3315756534707518E-4 28-29 0.0017754342046276692 30-31 0.0 32-33 2.6631513069415037E-4 34-35 0.0 36-37 0.0 38-39 4.438585511569173E-5 40-41 0.0 42-43 0.0 44-45 0.0 46-47 4.438585511569173E-5 48-49 7.101736818510677E-4 50-51 1.3315756534707518E-4 52-53 5.326302613883007E-4 54-55 0.0015535049290492104 56-57 2.6631513069415037E-4 58-59 9.76488812545218E-4 60-61 0.002973852292751346 62-63 0.0018642059148590527 64-65 0.0012871897983550602 66-67 0.009942431545914947 68-69 0.013715229230748744 70-71 0.024634149589208912 72-73 0.015357505870029339 74-75 0.017576798625813926 76-77 0.030182381478670375 78-79 0.019574162106020053 80-81 0.04358690972360928 82-83 0.03466535284535525 84-85 0.01850890158324345 86-87 0.013226984824476137 88-89 0.012960669693781986 90-91 0.023125030515275393 92-93 0.030581854174711605 94-95 0.012605582852856453 96-97 0.01979609138159851 98-99 0.027829931157538717 100-101 0.03959218276319702 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1126485.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.674208827571 #Duplication Level Percentage of deduplicated Percentage of total 1 75.30913816371569 37.40921855768809 2 13.192202420296596 13.106244358428013 3 4.643173213871307 6.91937867545282 4 2.1592904313985635 4.290441752346725 5 1.1990297343051655 2.9780426706170884 6 0.710636534418009 2.118018456670892 7 0.46880878630437367 1.63013938857585 8 0.35731470145218086 1.4199460077677457 9 0.2528794225397807 1.1305426719092968 >10 1.507377018096443 14.022813768880024 >50 0.13362648736655122 4.577854884574911 >100 0.06239901427988366 5.386571994433713 >500 0.00233099719224819 0.8317316468452232 >1k 0.0012551523342874868 0.970412967699583 >5k 1.7930747632678384E-4 0.7162084142517069 >10k+ 3.586149526535677E-4 2.492433783858415 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16549 1.4690830326191648 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11434 1.0150157347856386 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8041 0.7138133219705545 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2417 0.21456122362925384 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2092 0.1857104178040542 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1599 0.14194596465998216 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1525 0.1353768581028598 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1157 0.10270886873771067 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 8.877171023138346E-5 0.0 0.0 0.0 0.0 12-13 8.877171023138346E-5 0.0 0.0 0.0 0.0 14-15 8.877171023138346E-5 0.0 0.0 0.0 0.0 16-17 8.877171023138346E-5 0.0 0.0 1.331575653470752E-4 0.0 18-19 8.877171023138346E-5 0.0 0.0 2.6631513069415037E-4 0.0 20-21 1.331575653470752E-4 0.0 0.0 3.5508684092553386E-4 0.0 22-23 1.7754342046276693E-4 0.0 0.0 8.433312471981429E-4 0.0 24-25 2.2192927557845865E-4 0.0 0.0 0.001819820059743361 0.0 26-27 2.6631513069415037E-4 0.0 0.0 0.00230806446601597 0.0 28-29 2.6631513069415037E-4 0.0 0.0 0.004305427946222098 0.0 30-31 2.6631513069415037E-4 0.0 0.0 0.008522084182212812 0.0 32-33 2.6631513069415037E-4 0.0 0.0 0.012516811142625067 0.0 34-35 2.6631513069415037E-4 0.0 0.0 0.015756978566070563 0.0 36-37 2.6631513069415037E-4 0.0 0.0 0.02050626506344958 0.0 38-39 3.107009858098421E-4 0.0 0.0 0.029605365362166382 0.0 40-41 3.5508684092553386E-4 0.0 0.0 0.050733032397235645 0.0 42-43 3.5508684092553386E-4 0.0 0.0 0.06990772180721447 0.0 44-45 3.5508684092553386E-4 0.0 0.0 0.09165679081390343 0.0 46-47 3.5508684092553386E-4 0.0 0.0 0.13226984824476137 0.0 48-49 3.5508684092553386E-4 0.0 0.0 0.1630736316950514 0.0 50-51 3.5508684092553386E-4 0.0 0.0 0.20488510721403302 0.0 52-53 3.5508684092553386E-4 0.0 0.0 0.24998113601157584 0.0 54-55 3.5508684092553386E-4 0.0 0.0 0.30808222035801636 0.0 56-57 3.5508684092553386E-4 0.0 0.0 0.4144751150703294 0.0 58-59 3.5508684092553386E-4 0.0 0.0 0.5105261055406862 0.0 60-61 3.5508684092553386E-4 0.0 0.0 0.5972116805816323 0.0 62-63 3.5508684092553386E-4 0.0 0.0 0.7077324598197047 0.0 64-65 3.5508684092553386E-4 0.0 0.0 0.8111515022392664 0.0 66-67 3.5508684092553386E-4 0.0 0.0 0.9549172869589919 0.0 68-69 3.994726960412256E-4 0.0 0.0 1.1364998202372867 0.0 70-71 4.438585511569173E-4 0.0 0.0 1.2676156362490403 0.0 72-73 4.438585511569173E-4 0.0 0.0 1.4160863216110289 0.0 74-75 4.88244406272609E-4 0.0 0.0 1.6282950949191513 0.0 76-77 5.326302613883007E-4 0.0 0.0 1.8510233158896923 0.0 78-79 5.326302613883007E-4 0.0 0.0 2.0878218529319077 0.0 80-81 5.326302613883007E-4 0.0 0.0 2.338735091900913 0.0 82-83 5.326302613883007E-4 0.0 0.0 2.6293736711984623 0.0 84-85 5.326302613883007E-4 0.0 0.0 2.982640692064253 0.0 86-87 5.326302613883007E-4 0.0 0.0 3.3098532159771326 0.0 88-89 5.326302613883007E-4 0.0 0.0 3.7041771528249376 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5640 0.0 56.536392 1 GTATCAA 11060 0.0 44.942913 1 ATCAACG 14265 0.0 34.681847 3 TCAACGC 14390 0.0 34.347553 4 TATCAAC 14470 0.0 34.321873 2 CAACGCA 14705 0.0 33.57946 5 AACGCAG 15005 0.0 33.000183 6 ACGCAGA 16840 0.0 29.291388 7 CGCAGAG 16865 0.0 29.077608 8 GCAGAGT 17235 0.0 27.814299 9 GTACATG 10115 0.0 26.684994 1 TACATGG 10210 0.0 26.113214 2 GAGTACT 10330 0.0 25.75471 12-13 ACATGGG 10295 0.0 25.112837 3 GTACTTT 11055 0.0 23.827736 14-15 AGTACTT 10530 0.0 23.618767 12-13 CATGGGA 7365 0.0 23.230202 4 CAGAGTA 17130 0.0 22.520958 10-11 TAAGGTA 300 7.2759576E-12 22.17839 4 AGAGTAC 16640 0.0 22.084888 10-11 >>END_MODULE