FastQCFastQC Report
Thu 26 May 2016
SRR936965_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936965_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1126485
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167201.4842629950687314No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130771.1608676546958014No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95050.8437751057492998No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28420.2522892004775918No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26120.23187170712437363No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23760.21092158350976709No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19820.17594552967860203No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13660.12126215617606982No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12530.11123095291992346No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11730.1041292161014128No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA11360.10084466282285162No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69300.051.2044561
GTATCAA121700.040.242831
TATCAAC163800.029.896932
ATCAACG164700.029.7309193
TCAACGC166350.029.4074734
CAACGCA169850.028.8091625
AACGCAG173900.028.1108996
CATATAG3100.026.0453683
CGCAGAG194500.024.9626248
ACGCAGA194800.024.9485687
GTACATG96400.023.8500181
GCAGAGT202450.023.4661149
GAGTACT124700.023.3282912-13
TACATGG98600.023.1231062
AGTACTT126900.022.8677212-13
GTAAGGT4600.022.714764
ACATGGG98150.022.3076553
GTATAGG3900.021.9244291
GTACTTT132000.021.87625314-15
ATGGGAG27050.021.602145
Produced by FastQC (version 0.11.5)