##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936965_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1126485 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.07366897916972 31.0 31.0 34.0 31.0 34.0 2 32.17362681260736 33.0 31.0 34.0 30.0 34.0 3 32.23892461950226 34.0 31.0 34.0 30.0 34.0 4 35.96650465829549 37.0 35.0 37.0 35.0 37.0 5 35.88918272324975 37.0 35.0 37.0 35.0 37.0 6 35.9579142198964 37.0 35.0 37.0 35.0 37.0 7 35.899435855781476 37.0 35.0 37.0 35.0 37.0 8 35.872496304877565 37.0 35.0 37.0 35.0 37.0 9 37.49650017532413 39.0 37.0 39.0 35.0 39.0 10-11 37.416527073152324 39.0 37.0 39.0 34.0 39.0 12-13 37.30625574242001 39.0 37.0 39.0 34.0 39.0 14-15 38.3558613740973 40.0 38.0 41.0 33.5 41.0 16-17 38.29779313528365 40.0 38.0 41.0 33.5 41.0 18-19 38.55275036951224 40.0 38.0 41.0 34.0 41.0 20-21 38.615575440418645 40.0 38.0 41.0 34.0 41.0 22-23 38.519959431328424 40.0 38.0 41.0 34.0 41.0 24-25 38.393999476246904 40.0 38.0 41.0 34.0 41.0 26-27 38.16374962826846 40.0 37.0 41.0 33.5 41.0 28-29 38.02541622835635 40.0 37.0 41.0 33.0 41.0 30-31 37.84048433845102 40.0 37.0 41.0 33.0 41.0 32-33 37.50561125980373 40.0 35.5 41.0 33.0 41.0 34-35 37.269831378136416 39.0 35.0 41.0 33.0 41.0 36-37 37.02211569616995 39.0 35.0 41.0 32.0 41.0 38-39 36.785308725815256 39.0 35.0 41.0 31.5 41.0 40-41 36.49244330816656 39.0 35.0 41.0 31.0 41.0 42-43 36.081056117036624 39.0 35.0 41.0 30.0 41.0 44-45 35.55958046489745 39.0 35.0 40.0 28.0 41.0 46-47 34.84691052255467 38.0 35.0 40.0 24.5 41.0 48-49 34.21135700874845 38.0 34.0 40.0 21.0 41.0 50-51 33.81245200779416 38.0 34.0 40.0 16.5 41.0 52-53 33.51696915626928 38.0 33.0 40.0 14.0 41.0 54-55 33.20412078278894 38.0 33.0 40.0 9.0 41.0 56-57 32.90248116930097 37.5 33.0 40.0 7.5 41.0 58-59 32.540032490445945 37.0 32.5 40.0 6.0 41.0 60-61 32.18853912835058 36.0 32.0 39.5 2.0 41.0 62-63 31.803988069082145 36.0 31.0 39.0 2.0 40.0 64-65 31.40762726534308 35.0 31.0 39.0 2.0 40.0 66-67 31.00609151475608 35.0 31.0 38.5 2.0 40.0 68-69 30.705446588281248 35.0 30.0 38.0 2.0 40.0 70-71 30.24876629515706 35.0 29.5 37.5 2.0 39.5 72-73 29.742514103605462 34.0 29.0 37.0 2.0 39.0 74-75 29.22720409060041 34.0 28.5 36.0 2.0 39.0 76-77 27.881383684647375 32.5 26.5 35.0 2.0 37.0 78-79 28.595857468142054 34.0 28.5 35.0 2.0 37.5 80-81 28.371880673067107 34.0 28.0 35.0 2.0 37.0 82-83 28.043315712148853 34.0 27.5 35.0 2.0 36.5 84-85 27.675648144449326 34.0 27.0 35.0 2.0 36.0 86-87 27.290149447174173 33.0 26.0 35.0 2.0 36.0 88-89 26.869359556496534 33.0 25.5 35.0 2.0 35.0 90-91 26.393664806899338 33.0 25.0 35.0 2.0 35.0 92-93 25.88591104186918 32.5 23.5 34.5 2.0 35.0 94-95 25.270220642085782 32.0 20.0 34.0 2.0 35.0 96-97 24.557217361971087 32.0 14.0 34.0 2.0 35.0 98-99 23.69557339866931 31.0 2.0 34.0 2.0 35.0 100-101 20.395210766233017 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.07085255557736758 1101 2 0.11708648257098275 1101 3 0.1930058466450788 1101 4 0.1543400988755721 1101 5 0.16598340052100014 1101 6 0.23556960332587096 1101 7 0.09880604167644691 1101 8 0.18654275944325605 1101 9 0.20761312047311975 1101 10-11 0.2942392985585869 1101 12-13 0.24578178918972782 1101 14-15 0.44999265426793755 1101 16-17 0.5010326694420613 1101 18-19 0.35354995281739576 1101 20-21 0.35060612094610377 1101 22-23 0.25035128255739636 1101 24-25 0.42171660986172554 1101 26-27 0.18583705624227775 1101 28-29 0.3692532351280775 1101 30-31 0.4197864457177545 1101 32-33 0.49029438403002246 1101 34-35 0.5040557234252745 1101 36-37 0.5389884206211164 1101 38-39 0.5484907203715466 1101 40-41 0.4823788668238933 1101 42-43 0.3515593033989646 1101 44-45 0.3451836695760022 1101 46-47 0.4194103659831967 1101 48-49 0.762132833541159 1101 50-51 0.5568470307394406 1101 52-53 0.44881172692681304 1101 54-55 0.4948327644363033 1101 56-57 0.6000470252406345 1101 58-59 0.6104378110167161 1101 60-61 0.8510355228719604 1101 62-63 0.8363160905254006 1101 64-65 0.5958712118179363 1101 66-67 0.5056491733499442 1101 68-69 0.33654666484847695 1101 70-71 0.42951377289018566 1101 72-73 0.3969822472103921 1101 74-75 0.4435866968669977 1101 76-77 0.43921813158972967 1101 78-79 0.6341768737720344 1101 80-81 0.7830786950724082 1101 82-83 0.6511818466517525 1101 84-85 0.5168323409602884 1101 86-87 0.43610776619307856 1101 88-89 0.4600931378783706 1101 90-91 0.6165899665971857 1101 92-93 0.8936750931609083 1101 94-95 0.7141847804025225 1101 96-97 0.7078355778445307 1101 98-99 0.780532577448799 1101 100-101 1.0611044203623017 1102 1 0.28463844570351426 1102 2 0.2644990771193818 1102 3 0.2210444695867011 1102 4 0.1486384955387976 1102 5 0.22427240930937842 1102 6 0.22251841644848724 1102 7 0.2048227000521905 1102 8 0.2183901044349028 1102 9 0.337908149746454 1102 10-11 0.24579352756661166 1102 12-13 0.2460088329020209 1102 14-15 0.284092579011876 1102 16-17 0.38137935753757546 1102 18-19 0.3178747528786303 1102 20-21 0.345502739525422 1102 22-23 0.30156367047050736 1102 24-25 0.3054013507385207 1102 26-27 0.30527607969920467 1102 28-29 0.4281124042501361 1102 30-31 0.5033984815855632 1102 32-33 0.4476407787544474 1102 34-35 0.5535818653403481 1102 36-37 0.49308477389093497 1102 38-39 0.5737831348156064 1102 40-41 0.7061138634437469 1102 42-43 0.7880635955455517 1102 44-45 0.640272892652824 1102 46-47 0.7708140828673464 1102 48-49 0.8849826147883704 1102 50-51 0.6290265228248018 1102 52-53 0.8015726039788191 1102 54-55 0.6700505605816645 1102 56-57 0.8885978996140267 1102 58-59 0.7488056473411646 1102 60-61 0.8058474464934307 1102 62-63 0.9499272335246687 1102 64-65 0.9444266780866393 1102 66-67 0.7753924098708183 1102 68-69 0.52975065979739 1102 70-71 0.4141921488898781 1102 72-73 0.6992909501275193 1102 74-75 0.956145530525589 1102 76-77 0.7198888034095212 1102 78-79 0.8773202812134571 1102 80-81 1.0388970090815164 1102 82-83 0.8769602366822369 1102 84-85 0.8353274748267943 1102 86-87 0.7678428648550515 1102 88-89 0.8424245451667254 1102 90-91 1.0239395501377935 1102 92-93 0.9885602320019622 1102 94-95 0.899983068645998 1102 96-97 1.2136647338286686 1102 98-99 1.2285842107604452 1102 100-101 1.3350403124017411 1103 1 0.13187654663482107 1103 2 0.129610502068509 1103 3 0.17355679271033608 1103 4 0.17827526539839056 1103 5 0.12980218531372145 1103 6 0.033972507675720465 1103 7 0.05597281674513255 1103 8 0.06878601358128833 1103 9 -0.005027132371502319 1103 10-11 0.1375181670887784 1103 12-13 0.269703353937885 1103 14-15 0.412650094809635 1103 16-17 0.354552932376734 1103 18-19 0.24674486130218298 1103 20-21 0.15470990001371376 1103 22-23 0.26126138966519363 1103 24-25 0.1699329225937234 1103 26-27 0.2722864737153614 1103 28-29 0.16649504253189207 1103 30-31 0.15656762284006476 1103 32-33 0.2941206868945585 1103 34-35 0.32677810625835235 1103 36-37 0.20654991919045784 1103 38-39 0.20939279006703515 1103 40-41 0.13528012535901013 1103 42-43 0.3396483529811434 1103 44-45 0.5337116855222277 1103 46-47 0.3907044388244145 1103 48-49 0.41663143503141953 1103 50-51 0.6724499974101477 1103 52-53 0.5747708375191323 1103 54-55 0.6211996809634499 1103 56-57 0.5325023434108758 1103 58-59 0.621710467245471 1103 60-61 0.4499424347992331 1103 62-63 0.683322587227579 1103 64-65 0.49934683620121945 1103 66-67 0.5246956147547337 1103 68-69 0.5725671318097625 1103 70-71 0.5607649630454681 1103 72-73 0.4287750353021593 1103 74-75 0.7554237030916617 1103 76-77 0.5341305732566646 1103 78-79 0.7686249755825543 1103 80-81 0.8676886580013878 1103 82-83 0.7434216632503698 1103 84-85 0.6782483104329593 1103 86-87 0.6134395431536497 1103 88-89 0.5664184577055522 1103 90-91 0.5526851196666094 1103 92-93 0.6512565987042507 1103 94-95 0.5229121736347757 1103 96-97 0.6624931964458263 1103 98-99 0.4773992231769739 1103 100-101 0.35340606320937695 1104 1 0.2175281634934194 1104 2 0.1578118414275238 1104 3 0.2077421432913482 1104 4 0.11466736892416662 1104 5 0.08327481158732297 1104 6 0.06691681083651702 1104 7 0.08307249090818658 1104 8 0.12599373624934884 1104 9 0.21168494512303226 1104 10-11 0.25981193223915255 1104 12-13 0.14522981944772795 1104 14-15 0.10811382343790399 1104 16-17 0.2758602582766727 1104 18-19 0.2810968753257228 1104 20-21 0.26870199044450516 1104 22-23 0.20001331857132953 1104 24-25 0.2551185266420575 1104 26-27 0.15032454738357615 1104 28-29 0.17863468863992438 1104 30-31 0.28299523659158865 1104 32-33 0.3159898074820049 1104 34-35 0.24019818394533843 1104 36-37 0.29022736789060843 1104 38-39 0.128514394361396 1104 40-41 0.15271728803294593 1104 42-43 0.16873945687348169 1104 44-45 0.0620000767686264 1104 46-47 0.29248907691921033 1104 48-49 0.01698663649330001 1104 50-51 0.1040105971802916 1104 52-53 0.2053717336327452 1104 54-55 0.06611865326507171 1104 56-57 -0.07046590273913012 1104 58-59 0.09631165303601819 1104 60-61 0.1021312069410385 1104 62-63 0.19120189908613128 1104 64-65 0.3065735317165341 1104 66-67 0.4508431247979452 1104 68-69 0.4652460432792367 1104 70-71 0.14668046368806387 1104 72-73 0.11367622024947721 1104 74-75 0.1617935699444324 1104 76-77 0.31845741415607165 1104 78-79 0.2744152901548418 1104 80-81 0.37918620343577913 1104 82-83 0.5058459020671755 1104 84-85 0.36788179470583415 1104 86-87 0.24660611384003062 1104 88-89 0.0560538268625983 1104 90-91 0.09175132820721998 1104 92-93 0.14367217201409233 1104 94-95 0.22751486237244123 1104 96-97 0.28695953861480206 1104 98-99 0.38018483397121017 1104 100-101 0.6056841689404671 1105 1 0.1347763595086633 1105 2 0.13254590153292867 1105 3 0.14790528626075883 1105 4 0.07033965340293946 1105 5 0.08274633314832869 1105 6 0.042696659207372534 1105 7 0.1372204628404461 1105 8 0.0335963123405989 1105 9 0.2832346307265894 1105 10-11 0.16216830642016333 1105 12-13 0.13125001636051792 1105 14-15 0.1521569931713671 1105 16-17 0.117847489506552 1105 18-19 0.23913081369325795 1105 20-21 0.21587064563187397 1105 22-23 0.2743597262886439 1105 24-25 0.11355585498809972 1105 26-27 0.193794564227872 1105 28-29 0.2139467955174581 1105 30-31 0.28912223590014463 1105 32-33 0.2268165589489044 1105 34-35 0.04018026339885239 1105 36-37 0.24347174830167972 1105 38-39 0.3825744388530907 1105 40-41 0.4280324713555643 1105 42-43 0.4996088419804394 1105 44-45 0.45128968193786534 1105 46-47 0.34318645523971725 1105 48-49 0.39841480759238124 1105 50-51 0.32870404414918397 1105 52-53 0.346492991259133 1105 54-55 0.3969715392853246 1105 56-57 0.44613319382165173 1105 58-59 0.288209992263333 1105 60-61 0.37525581921250506 1105 62-63 0.35406235336436964 1105 64-65 0.13539931456012155 1105 66-67 0.08429129177479311 1105 68-69 0.013323366629862221 1105 70-71 -0.09186790097323083 1105 72-73 -0.12010314685571188 1105 74-75 0.07726240291580311 1105 76-77 0.025133096939047306 1105 78-79 0.16005275902233151 1105 80-81 0.17423327913764908 1105 82-83 0.2307721130747602 1105 84-85 0.47623485079359185 1105 86-87 0.47477985277675927 1105 88-89 0.4147622478949238 1105 90-91 0.37304425756168413 1105 92-93 0.12149409965569546 1105 94-95 -0.0343350355362837 1105 96-97 0.15362991123017622 1105 98-99 0.29944921838266936 1105 100-101 0.5551555889686277 1106 1 0.08432073450140365 1106 2 0.07723147328449897 1106 3 -0.027587695878573015 1106 4 0.114301737629944 1106 5 0.13243950305014351 1106 6 0.11634380301042313 1106 7 0.091664826322436 1106 8 0.17427614350220466 1106 9 0.061523351987993635 1106 10-11 0.10539391392897102 1106 12-13 0.18664074292109945 1106 14-15 0.16744068032822668 1106 16-17 0.14801489169951054 1106 18-19 0.05596417501144657 1106 20-21 0.02759835622840967 1106 22-23 0.05930408924260888 1106 24-25 0.12616593312390734 1106 26-27 0.18812446440117014 1106 28-29 0.015080657077994886 1106 30-31 0.204793856412941 1106 32-33 0.2186460393494727 1106 34-35 0.1039933731913294 1106 36-37 0.33801855744818 1106 38-39 0.38861018976049166 1106 40-41 0.32932753620121247 1106 42-43 0.2219054264561393 1106 44-45 -0.2429626526848807 1106 46-47 -0.026835013890888604 1106 48-49 0.1132668944700157 1106 50-51 0.17310855298341465 1106 52-53 -0.03196907765540402 1106 54-55 -0.20903717720282344 1106 56-57 -0.10532407753949968 1106 58-59 0.04327683380890335 1106 60-61 -0.03657897302904445 1106 62-63 0.17471828652176669 1106 64-65 -0.19361484788505479 1106 66-67 0.040832934812744526 1106 68-69 0.11658639991281916 1106 70-71 -0.23148641997282837 1106 72-73 -0.06680415568308007 1106 74-75 0.0018743903574716114 1106 76-77 0.08495414175598981 1106 78-79 0.25026693287265545 1106 80-81 0.12578543497417982 1106 82-83 0.4467074247784417 1106 84-85 0.37625634104483296 1106 86-87 0.2864185926771299 1106 88-89 0.42939040318654165 1106 90-91 0.5272547627568365 1106 92-93 0.7695590266152408 1106 94-95 0.6628068666519198 1106 96-97 0.5596274865806983 1106 98-99 0.13568162356947866 1106 100-101 0.5233281044685398 1107 1 0.1064035177100422 1107 2 0.12174211791950285 1107 3 0.08700716784609597 1107 4 0.11558202782905624 1107 5 0.006672662566302279 1107 6 -0.01639461769827477 1107 7 0.16337441382894013 1107 8 0.12662256424646046 1107 9 0.138492464986939 1107 10-11 0.1525740525381707 1107 12-13 0.2305762571932206 1107 14-15 0.27538848182989994 1107 16-17 0.12403195556732527 1107 18-19 0.16175232032437492 1107 20-21 0.11686914649642688 1107 22-23 0.10427706089396338 1107 24-25 0.12636292221920797 1107 26-27 0.0615932840002813 1107 28-29 0.23781531026605052 1107 30-31 0.19655115473388918 1107 32-33 0.26988848763775053 1107 34-35 0.19149126160159824 1107 36-37 0.09569085205718153 1107 38-39 0.20245938446684164 1107 40-41 0.17202049104875528 1107 42-43 0.1985818064355982 1107 44-45 -0.00681384784466843 1107 46-47 0.1767355732199718 1107 48-49 0.2409805651242607 1107 50-51 0.24027794471221142 1107 52-53 0.25670830032805014 1107 54-55 0.12650110036231155 1107 56-57 0.29862220584028876 1107 58-59 0.26545997309825253 1107 60-61 0.3404009201911933 1107 62-63 0.4103161331447822 1107 64-65 0.08817708050760231 1107 66-67 0.22167725952634498 1107 68-69 0.24911032755864326 1107 70-71 0.36688032093288925 1107 72-73 0.3075529208098118 1107 74-75 0.23107550927776188 1107 76-77 0.1716624067404986 1107 78-79 0.37906973928655674 1107 80-81 0.46685802253847086 1107 82-83 0.4383299703332142 1107 84-85 0.41804859173276654 1107 86-87 0.30831299985386806 1107 88-89 0.37343966546376706 1107 90-91 0.47388294292211697 1107 92-93 0.335940739803565 1107 94-95 0.32900620516864976 1107 96-97 0.3060455635670394 1107 98-99 -0.012266077909526274 1107 100-101 0.43694269044135936 1108 1 -0.00997142471280199 1108 2 -0.0662012261615672 1108 3 -0.1137666690954049 1108 4 -0.0625939279516956 1108 5 -0.0022564023807234435 1108 6 -0.037635981125262674 1108 7 0.04025142902930412 1108 8 0.013838133108734496 1108 9 0.06222576761246046 1108 10-11 0.02434742886244834 1108 12-13 0.10449380670957709 1108 14-15 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-0.7209497594345038 2316 72-73 -0.5498808912446123 2316 74-75 -0.6910342035583348 2316 76-77 -0.32289397418307786 2316 78-79 -0.4516074539733097 2316 80-81 -0.3540353514483421 2316 82-83 -0.43112629509230516 2316 84-85 -0.5477394074697486 2316 86-87 -0.5962197830304206 2316 88-89 -1.0455170537040956 2316 90-91 -0.9895035733679229 2316 92-93 -1.0677730725258527 2316 94-95 -0.9555375087811768 2316 96-97 -0.6821399483024457 2316 98-99 -0.6904085014436312 2316 100-101 -1.232918075970705 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 4.0 4 16.0 5 26.0 6 89.0 7 225.0 8 546.0 9 1094.0 10 1791.0 11 3223.0 12 4906.0 13 6962.0 14 8898.0 15 12713.0 16 17712.0 17 25071.0 18 20454.0 19 10720.0 20 8359.0 21 8172.0 22 8455.0 23 8858.0 24 9851.0 25 11546.0 26 13761.0 27 16445.0 28 20355.0 29 24919.0 30 30063.0 31 36890.0 32 47041.0 33 60488.0 34 82046.0 35 114748.0 36 161926.0 37 214427.0 38 127508.0 39 6177.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.745488191689255 20.151204628843605 15.791684278199469 19.311622901267675 2 15.822241476811191 22.49244735788471 39.776922960831016 21.90838820447308 3 18.559889496778915 28.449075574154246 31.809889718708583 21.181145210358256 4 12.214188382446283 18.681029929382102 37.77768900606755 31.32709268210407 5 12.05493193429118 39.7100715943843 32.96306652995823 15.271929941366286 6 28.06002743045846 38.67783414781377 17.592244903394185 15.669893518333577 7 25.65697723449491 36.85792531636019 20.77586474742229 16.709232701722616 8 27.423800583230136 33.22955920407284 19.670745726751797 19.675894485945218 9 28.487640758643035 15.75280629568969 20.89881356609276 34.86073937957452 10-11 24.35818441388715 28.31388679754531 26.235244190010796 21.092684598556737 12-13 26.78748496429158 26.541454169385297 27.693622196478422 18.977438669844695 14-15 23.4455407750658 26.117169780334404 28.713032130920517 21.724257313679278 16-17 19.72906874037382 29.851351771217548 28.988490747768502 21.431088740640135 18-19 19.703137337929952 26.18926825630803 34.75279865030952 19.354795755452493 20-21 21.424608356540027 24.903126667264402 35.137966268106325 18.534298708089246 22-23 20.697041751574954 25.340790910999544 35.08565102815412 18.876516309271384 24-25 20.155705656115828 25.549426646637475 35.44172535148745 18.853142345759245 26-27 19.98591990681926 25.739093920258377 35.23108309296856 19.0439030799538 28-29 19.628399971592927 25.68381151906825 35.41048221007031 19.277306299268517 30-31 19.74475587486717 25.818477679011338 35.326525521305406 19.110240924816082 32-33 19.6075681074183 25.983021694501467 35.00318481939744 19.406225378682795 34-35 19.839811448887467 25.856402881529718 34.983688198244984 19.320097471337835 36-37 20.3442315426321 25.645223292403273 34.520278446332675 19.49026671863195 38-39 20.043575692789588 25.927004499625326 34.56236970796828 19.467050099616802 40-41 19.989036572131145 26.019410149826115 34.50621297901191 19.485340299030824 42-43 20.08477698327097 26.319214192821033 33.87430813548338 19.72170068842461 44-45 20.161919599462045 26.66320456996764 33.326409139935286 19.84846669063503 46-47 20.639710105559004 26.97657923494357 32.23717441692211 20.146536242575316 48-49 20.857191556452772 27.37041988697195 31.334791015281695 20.437597541293588 50-51 21.11854040739651 27.78031536852254 30.694076197857505 20.40706802622345 52-53 21.111199882821342 28.077604229084272 30.552559510335247 20.258636377759135 54-55 20.79621427804159 28.09787090869223 30.44224164027829 20.66367317298789 56-57 20.927855231963807 28.457929053257775 29.922192684629806 20.692023030148615 58-59 20.980240199772922 27.91756437536785 30.26221527431936 20.83998015053987 60-61 21.02909707324803 27.81228874516347 30.61657671621598 20.542037465372516 62-63 21.066209761900534 27.858924735001157 30.781591235951065 20.293274267147247 64-65 21.015550618851158 27.947482539675494 30.593861866473738 20.443104974999613 66-67 20.99903547096581 28.245653516136667 30.196239268893205 20.559071744004317 68-69 21.158256600775427 28.155733248111925 30.25617466837997 20.429835482732678 70-71 20.9787540402655 28.2951744071984 30.147016395421815 20.579055157114283 72-73 21.102633412784012 28.330692374953948 29.651304722211126 20.91536949005091 74-75 20.998691060551657 28.660580771417628 29.51123606228048 20.829492105750237 76-77 20.890735340461703 28.730253842705405 29.72001402593022 20.658996790902677 78-79 20.859971068567674 28.630488616293658 29.50176060948647 21.007779705652194 80-81 20.683355282694006 29.019229770523765 29.327186624605567 20.970228322176666 82-83 20.778855266983772 28.912332725904648 29.064907565039633 21.243904442071944 84-85 20.703074714506016 28.7287338831177 29.059572205141954 21.508619197234328 86-87 20.796559755298034 28.57053447102642 29.013038076438736 21.619867697236806 88-89 20.744825578118004 28.52344718518818 29.002950344813506 21.728776891880315 90-91 20.424279089958013 28.698981913055366 29.221496606006962 21.655242390979655 92-93 20.295282388251554 28.71837163698278 29.037684612523424 21.948661362242238 94-95 20.179372197309416 28.55454291648043 29.05135401330422 22.214730872905932 96-97 20.630052115213637 28.411602326430952 28.729415911008644 22.228929647346767 98-99 20.294560733343573 28.45519180936334 28.696301767399913 22.55394568989318 100-101 20.276995351009028 28.713577947608528 28.723964274586077 22.285462426796368 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2110.0 1 1498.0 2 1041.5 3 1205.5 4 1026.0 5 996.0 6 1745.0 7 3680.0 8 5971.0 9 6876.0 10 5658.5 11 4259.0 12 4125.5 13 4237.0 14 4375.0 15 4497.0 16 4548.0 17 4674.0 18 4833.5 19 4958.0 20 5273.5 21 5755.5 22 6367.0 23 7141.5 24 7942.5 25 9235.0 26 10823.0 27 12203.5 28 13333.5 29 14801.5 30 16941.5 31 18868.5 32 21484.5 33 24018.0 34 26506.5 35 28474.0 36 31010.0 37 34094.0 38 34605.5 39 33780.5 40 32945.0 41 33115.0 42 33162.5 43 33992.5 44 35541.5 45 36084.5 46 35334.5 47 34436.0 48 36938.0 49 36718.5 50 33055.0 51 30823.0 52 29239.5 53 29094.0 54 28543.0 55 26721.5 56 24414.0 57 22199.5 58 21569.0 59 20484.0 60 17764.5 61 15073.5 62 12652.0 63 9515.0 64 6380.5 65 4649.0 66 3278.5 67 2567.5 68 1924.0 69 1119.5 70 763.0 71 574.5 72 479.0 73 422.0 74 322.0 75 251.5 76 180.5 77 86.0 78 52.5 79 42.0 80 27.0 81 14.5 82 8.0 83 5.5 84 2.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.029205892666125156 2 0.005148759193420241 3 1.7754342046276693E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.013226984824476137 12-13 0.0 14-15 0.0 16-17 0.0 18-19 3.994726960412256E-4 20-21 0.0012428039432393683 22-23 0.002174906900668895 24-25 0.011451550619848467 26-27 0.006391563136659609 28-29 4.438585511569173E-4 30-31 0.0030182381478670375 32-33 0.004216656235990715 34-35 0.0 36-37 0.008166997341287278 38-39 0.015002419029103804 40-41 0.0011096463778922934 42-43 0.0 44-45 0.0 46-47 5.326302613883007E-4 48-49 0.0034620966990239555 50-51 0.007856296355477436 52-53 0.0 54-55 0.0037284118297181055 56-57 0.004305427946222098 58-59 5.326302613883007E-4 60-61 0.002796308872288579 62-63 0.005770161165039925 64-65 2.2192927557845865E-4 66-67 0.0025299937415944286 68-69 6.65787826735376E-4 70-71 0.0010652605227766015 72-73 0.0 74-75 4.438585511569173E-5 76-77 0.0 78-79 0.0025299937415944286 80-81 0.0025299937415944286 82-83 0.013537685810285978 84-85 0.00923225786406388 86-87 0.004749286497379015 88-89 5.770161165039925E-4 90-91 8.433312471981429E-4 92-93 3.5508684092553386E-4 94-95 4.438585511569173E-5 96-97 0.003417710843908263 98-99 0.0024856078864787367 100-101 3.5508684092553386E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1126485.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.284520359907226 #Duplication Level Percentage of deduplicated Percentage of total 1 74.58026882694017 36.01072508621746 2 13.460635307739027 12.998806391476222 3 4.796945987220183 6.9485470855592455 4 2.2393944989826373 4.325123571199656 5 1.2558205530370192 3.031834653075296 6 0.7578233065533301 2.195468092469189 7 0.4983768138261857 1.684471978986731 8 0.35950812055730585 1.3886941733280973 9 0.2813676925360182 1.222713367098794 >10 1.5597912299226615 14.135268243455497 >50 0.13711831806698405 4.570780815630288 >100 0.06798805491435984 5.742630144108468 >500 0.002940023528453965 0.9501401349740318 >1k 0.0014700117642269825 1.3077846287718022 >5k 1.837514705283728E-4 0.8433170214705549 >10k+ 3.675029410567456E-4 2.6436946121786558 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16720 1.4842629950687314 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13077 1.1608676546958014 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9505 0.8437751057492998 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2842 0.2522892004775918 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2612 0.23187170712437363 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2376 0.21092158350976709 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1982 0.17594552967860203 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1366 0.12126215617606982 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1253 0.11123095291992346 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1173 0.1041292161014128 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1136 0.10084466282285162 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 2.6631513069415037E-4 0.0 0.0 0.0 0.0 12-13 2.6631513069415037E-4 0.0 0.0 0.0 0.0 14-15 2.6631513069415037E-4 0.0 0.0 0.0 0.0 16-17 2.6631513069415037E-4 0.0 0.0 1.331575653470752E-4 0.0 18-19 2.6631513069415037E-4 0.0 0.0 2.6631513069415037E-4 0.0 20-21 2.6631513069415037E-4 0.0 0.0 3.5508684092553386E-4 0.0 22-23 2.6631513069415037E-4 0.0 0.0 8.433312471981429E-4 0.0 24-25 2.6631513069415037E-4 0.0 0.0 0.001819820059743361 0.0 26-27 2.6631513069415037E-4 0.0 0.0 0.002352450321131662 0.0 28-29 2.6631513069415037E-4 0.0 0.0 0.004571743076916248 0.0 30-31 2.6631513069415037E-4 0.0 0.0 0.009010328588485422 0.0 32-33 2.6631513069415037E-4 0.0 0.0 0.013226984824476137 0.0 34-35 3.107009858098421E-4 0.0 0.0 0.016822239088847165 0.0 36-37 3.5508684092553386E-4 0.0 0.0 0.022015384137383097 0.0 38-39 3.5508684092553386E-4 0.0 0.0 0.031602728842372516 0.0 40-41 3.5508684092553386E-4 0.0 0.0 0.05344056955929284 0.0 42-43 3.5508684092553386E-4 0.0 0.0 0.0734585902164698 0.0 44-45 3.5508684092553386E-4 0.0 0.0 0.09565151777431569 0.0 46-47 3.5508684092553386E-4 0.0 0.0 0.13715229230748743 0.0 48-49 4.438585511569173E-4 0.0 0.0 0.16875502114985996 0.0 50-51 4.438585511569173E-4 0.0 0.0 0.2108771976546514 0.0 52-53 4.438585511569173E-4 0.0 0.0 0.25499673763964903 0.0 54-55 4.438585511569173E-4 0.0 0.0 0.3132753654065522 0.0 56-57 4.438585511569173E-4 0.0 0.0 0.41997896110467514 0.0 58-59 4.438585511569173E-4 0.0 0.0 0.5168288969671145 0.0 60-61 4.88244406272609E-4 0.0 0.0 0.6040914881245645 0.0 62-63 5.326302613883007E-4 0.0 0.0 0.7156775278854135 0.0 64-65 6.214019716196843E-4 0.0 0.0 0.8190965703049753 0.0 66-67 6.214019716196843E-4 0.0 0.0 0.9632618277207419 0.0 68-69 6.214019716196843E-4 0.0 0.0 1.1451994478399623 0.0 70-71 6.214019716196843E-4 0.0 0.0 1.2758714053005589 0.0 72-73 6.214019716196843E-4 0.0 0.0 1.4247859492137045 0.0 74-75 7.101736818510677E-4 0.0 0.0 1.6365508639706698 0.0 76-77 7.989453920824512E-4 0.0 0.0 1.8592346990860953 0.0 78-79 7.989453920824512E-4 0.0 0.0 2.095278676591344 0.0 80-81 8.433312471981429E-4 0.0 0.0 2.3455705135887293 0.0 82-83 8.877171023138346E-4 0.0 0.0 2.636342250451626 0.0 84-85 8.877171023138346E-4 0.0 0.0 2.9897868147378794 0.0 86-87 8.877171023138346E-4 0.0 0.0 3.3162891649689077 0.0 88-89 8.877171023138346E-4 0.0 0.0 3.7102580149757873 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6930 0.0 51.204456 1 GTATCAA 12170 0.0 40.24283 1 TATCAAC 16380 0.0 29.89693 2 ATCAACG 16470 0.0 29.730919 3 TCAACGC 16635 0.0 29.407473 4 CAACGCA 16985 0.0 28.809162 5 AACGCAG 17390 0.0 28.110899 6 CATATAG 310 0.0 26.045368 3 CGCAGAG 19450 0.0 24.962624 8 ACGCAGA 19480 0.0 24.948568 7 GTACATG 9640 0.0 23.850018 1 GCAGAGT 20245 0.0 23.466114 9 GAGTACT 12470 0.0 23.32829 12-13 TACATGG 9860 0.0 23.123106 2 AGTACTT 12690 0.0 22.86772 12-13 GTAAGGT 460 0.0 22.71476 4 ACATGGG 9815 0.0 22.307655 3 GTATAGG 390 0.0 21.924429 1 GTACTTT 13200 0.0 21.876253 14-15 ATGGGAG 2705 0.0 21.60214 5 >>END_MODULE