FastQCFastQC Report
Thu 26 May 2016
SRR936964_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936964_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1148397
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165091.437569063660041No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117741.0252552035576548No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82910.7219628752077896No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24170.21046728613885266No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22180.19313878388745354No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16300.14193697824010337No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15780.1374089274005418No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11990.10440640301219875No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60500.049.649431
GTATCAA115050.044.326731
ATCAACG149050.034.15453
TATCAAC149700.034.00622
TCAACGC150500.033.7953264
CAACGCA153050.033.139085
AACGCAG154850.032.7188876
GCGTATC804.4779724E-429.7052081
ACGCAGA173500.029.1407057
CGCAGAG174000.028.8670628
GCAGAGT179450.027.3548099
GTACATG101400.025.7796691
TACATGG103000.025.0583822
GAGTACT105050.024.70081512-13
AGTACTT107650.024.23667312-13
ACATGGG101850.023.9412463
ATGGGAG24900.023.2900185
GTACTTT110900.023.2249114-15
TATTCCG1252.2448221E-422.816585
CGTATCA1252.245485E-422.8155882
Produced by FastQC (version 0.11.5)