##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936964_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1148397 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.865118073279536 33.0 31.0 34.0 30.0 34.0 2 31.918894772452383 33.0 31.0 34.0 30.0 34.0 3 31.90218278173837 33.0 31.0 34.0 30.0 34.0 4 35.565257484998654 37.0 35.0 37.0 33.0 37.0 5 35.58298393325653 37.0 35.0 37.0 33.0 37.0 6 35.681101570275786 37.0 35.0 37.0 33.0 37.0 7 35.63952883889456 37.0 35.0 37.0 33.0 37.0 8 35.60860921789242 37.0 35.0 37.0 33.0 37.0 9 37.214859495453226 39.0 37.0 39.0 34.0 39.0 10-11 37.10075958052833 39.0 37.0 39.0 33.0 39.0 12-13 37.01385452940055 39.0 37.0 39.0 33.0 39.0 14-15 38.06480685686222 40.0 38.0 41.0 33.0 41.0 16-17 38.04028572000798 40.0 38.0 41.0 33.0 41.0 18-19 38.119077723121876 40.0 38.0 41.0 33.0 41.0 20-21 38.11557153144775 40.0 38.0 41.0 33.0 41.0 22-23 38.0068216827456 40.0 38.0 41.0 33.0 41.0 24-25 37.944653721665944 40.0 38.0 41.0 33.0 41.0 26-27 37.90824732213686 40.0 37.0 41.0 33.0 41.0 28-29 37.79771585958514 40.0 37.0 41.0 33.0 41.0 30-31 37.51928340112348 39.5 37.0 41.0 33.0 41.0 32-33 37.19009149275033 39.0 35.5 41.0 32.0 41.0 34-35 36.96060944081185 39.0 35.0 41.0 31.5 41.0 36-37 36.71538152746829 39.0 35.0 41.0 31.0 41.0 38-39 36.50030694960018 39.0 35.0 41.0 31.0 41.0 40-41 36.222944678538866 39.0 35.0 41.0 30.0 41.0 42-43 35.876426880251344 39.0 35.0 40.0 29.5 41.0 44-45 35.4101068707076 38.5 35.0 40.0 27.5 41.0 46-47 34.77086713044356 38.0 35.0 40.0 24.0 41.0 48-49 34.2628368064354 38.0 34.0 40.0 21.0 41.0 50-51 33.46067997391146 37.5 33.5 39.5 18.0 40.5 52-53 33.60583012668964 38.0 33.5 39.5 18.0 40.5 54-55 33.95028374333963 38.0 34.0 40.0 16.0 41.0 56-57 33.83206373754024 38.0 34.0 40.0 13.5 41.0 58-59 33.606596847605836 38.0 34.0 40.0 11.0 41.0 60-61 33.334037358160984 38.0 34.0 40.0 8.0 41.0 62-63 33.022293248763276 37.0 33.0 40.0 7.0 41.0 64-65 32.686471664415706 36.5 33.0 40.0 3.5 41.0 66-67 32.33358803619306 36.0 33.0 39.0 2.0 41.0 68-69 31.95136655703559 35.5 32.0 39.0 2.0 41.0 70-71 31.543048701799115 35.0 32.0 39.0 2.0 40.5 72-73 31.17578459365533 35.0 31.0 38.0 2.0 40.0 74-75 30.796235099882708 35.0 31.0 37.0 2.0 39.5 76-77 30.353743087103155 35.0 31.0 37.0 2.0 39.0 78-79 29.910518749178202 35.0 30.0 36.0 2.0 39.0 80-81 29.503098231709068 35.0 30.0 36.0 2.0 37.5 82-83 29.112720165587337 34.0 29.0 35.0 2.0 37.0 84-85 28.74828434766026 34.0 29.0 35.0 2.0 37.0 86-87 28.400109456921257 34.0 29.0 35.0 2.0 36.0 88-89 28.037441320379624 34.0 28.5 35.0 2.0 36.0 90-91 27.67328066861895 34.0 27.0 35.0 2.0 35.5 92-93 27.318101231542748 34.0 26.5 35.0 2.0 35.0 94-95 26.970861122068413 34.0 26.0 35.0 2.0 35.0 96-97 26.51566574973637 34.0 25.0 35.0 2.0 35.0 98-99 25.99937739300956 33.0 24.0 35.0 2.0 35.0 100-101 24.01233502003227 31.0 10.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2976163482415721 1101 2 0.22371776269465116 1101 3 0.33918021363442463 1101 4 0.30161336770317604 1101 5 0.2712003317103253 1101 6 0.25666593556613293 1101 7 0.3067150306280837 1101 8 0.15785804636012557 1101 9 0.15018703649037946 1101 10-11 0.3397539425049274 1101 12-13 0.4378023169998997 1101 14-15 0.45654949615032336 1101 16-17 0.440740749397591 1101 18-19 0.5474444157997311 1101 20-21 0.4796567627489381 1101 22-23 0.43072706339534506 1101 24-25 0.5644819133080645 1101 26-27 0.5139430683953776 1101 28-29 0.4608023162327086 1101 30-31 0.5130269306598478 1101 32-33 0.288507211209847 1101 34-35 0.2368061004716182 1101 36-37 0.39929826273912994 1101 38-39 0.3235564542625582 1101 40-41 0.36121559246151236 1101 42-43 0.33421607936240605 1101 44-45 0.41383280598182637 1101 46-47 0.3726366139635999 1101 48-49 0.511199059779166 1101 50-51 0.4123898743868324 1101 52-53 0.44729002612595536 1101 54-55 0.4299195538790954 1101 56-57 0.37638032428078105 1101 58-59 0.3572065524964927 1101 60-61 0.5270138147900596 1101 62-63 0.4294579374796541 1101 64-65 0.2545068283602916 1101 66-67 0.38209754541296803 1101 68-69 0.2550405951403043 1101 70-71 0.26043362061423636 1101 72-73 0.1576793863821493 1101 74-75 0.42138026587424093 1101 76-77 0.4183619107422949 1101 78-79 0.3842825041473752 1101 80-81 0.37379606253566067 1101 82-83 0.32171807647645423 1101 84-85 0.26388189797528483 1101 86-87 0.4699214800595648 1101 88-89 0.4564271550930137 1101 90-91 0.42925105834939714 1101 92-93 0.41904891198785066 1101 94-95 0.629255308514864 1101 96-97 0.6939320895339627 1101 98-99 0.5123803308671064 1101 100-101 0.5538222784975595 1102 1 0.18910620467079653 1102 2 0.11822077661201291 1102 3 0.13376271047208022 1102 4 0.17492904154255484 1102 5 0.2332151958776123 1102 6 0.08687542295955808 1102 7 0.0135339964990564 1102 8 0.005465124139405475 1102 9 0.14413741868214913 1102 10-11 0.13674422978324685 1102 12-13 0.13610257804227643 1102 14-15 0.13376297390042424 1102 16-17 0.46562310808997154 1102 18-19 0.11616218760320152 1102 20-21 0.12223412688456392 1102 22-23 0.13802449719630516 1102 24-25 0.1408356685341019 1102 26-27 0.23988580725826836 1102 28-29 0.2667545687300432 1102 30-31 0.06765307471491155 1102 32-33 0.1681255412174636 1102 34-35 0.30858186238556584 1102 36-37 0.39908955041816085 1102 38-39 0.24984149146350632 1102 40-41 0.041548103610146825 1102 42-43 0.3478573545541579 1102 44-45 0.5010646777716659 1102 46-47 0.2512091027415124 1102 48-49 0.22228548339730025 1102 50-51 0.32692597832556913 1102 52-53 0.514916110910768 1102 54-55 0.18943584787775336 1102 56-57 0.17828674922616017 1102 58-59 -0.13545356202172343 1102 60-61 -0.06286267513475963 1102 62-63 -0.1406858663226629 1102 64-65 0.1258534128996729 1102 66-67 -0.2161725232645253 1102 68-69 0.11536519345773755 1102 70-71 0.16658045175837088 1102 72-73 0.17903060615875432 1102 74-75 0.1875510381452905 1102 76-77 0.19555593569355167 1102 78-79 0.09097279785930823 1102 80-81 0.14362360638171268 1102 82-83 0.15190274128801207 1102 84-85 0.18549547724467885 1102 86-87 0.24269733873093102 1102 88-89 0.34629569165997154 1102 90-91 0.26424015920962773 1102 92-93 0.3979948033186389 1102 94-95 0.32554215421820487 1102 96-97 -0.017322013157563987 1102 98-99 0.08230959664046011 1102 100-101 0.10318198253133204 1103 1 0.19164365592502364 1103 2 0.23916323942785667 1103 3 0.2998074154027499 1103 4 0.1694439495456379 1103 5 0.10660591758216498 1103 6 0.22798394574319758 1103 7 0.16146883551399327 1103 8 0.13149870625743176 1103 9 0.1579507881884723 1103 10-11 0.23083764423871145 1103 12-13 0.10503525545399839 1103 14-15 0.4327221896181257 1103 16-17 0.5348397224813297 1103 18-19 0.4484130499550574 1103 20-21 0.372008999630431 1103 22-23 0.2901139356755138 1103 24-25 0.46490802023629385 1103 26-27 0.5043445097621415 1103 28-29 0.5069250453893019 1103 30-31 0.468061928702717 1103 32-33 0.4690140784245145 1103 34-35 0.4354358234805318 1103 36-37 0.32322428907976075 1103 38-39 0.464500566762851 1103 40-41 0.3544699787293908 1103 42-43 0.2636673929402704 1103 44-45 0.5638365475489024 1103 46-47 0.43178071610262236 1103 48-49 0.48852660231790423 1103 50-51 0.431969782747224 1103 52-53 0.43891539164827265 1103 54-55 0.5236515655758112 1103 56-57 0.5587219984305847 1103 58-59 0.5696347431897806 1103 60-61 0.5762132633406267 1103 62-63 0.49036705193706354 1103 64-65 0.529899836810479 1103 66-67 0.5215263232444798 1103 68-69 0.5287024092822783 1103 70-71 0.43585339565970926 1103 72-73 0.377840388891876 1103 74-75 0.45057312351049106 1103 76-77 0.517049340228148 1103 78-79 0.5174491938390666 1103 80-81 0.6619571686580166 1103 82-83 0.624349693293933 1103 84-85 0.42883967582980986 1103 86-87 0.6321932444674765 1103 88-89 0.5007193945072537 1103 90-91 0.6244349419809048 1103 92-93 0.6436897215478368 1103 94-95 0.7157329972625348 1103 96-97 0.5526634680424038 1103 98-99 0.6317636630491883 1103 100-101 0.7207544525207226 1104 1 0.22292014724702014 1104 2 0.13407077077127383 1104 3 0.15172371397946804 1104 4 0.16222715572062896 1104 5 0.14693965246816987 1104 6 0.12478769802982015 1104 7 0.0370795302001099 1104 8 0.13490911539498995 1104 9 0.25655148867480193 1104 10-11 0.3085617887084311 1104 12-13 0.15052085532907 1104 14-15 0.11070065077905866 1104 16-17 0.24085018515924617 1104 18-19 0.1440514629296743 1104 20-21 0.11685574036125956 1104 22-23 0.0518105514788374 1104 24-25 -0.0017170968832402878 1104 26-27 0.08213976251724375 1104 28-29 0.1313073793026618 1104 30-31 0.35406001017329203 1104 32-33 -0.02064733459471313 1104 34-35 -0.05633513533801704 1104 36-37 8.513916541588173E-4 1104 38-39 0.166580553823934 1104 40-41 0.056580639952784395 1104 42-43 0.26548553419757326 1104 44-45 0.15972119039011545 1104 46-47 0.2564444157412211 1104 48-49 0.31094272242677334 1104 50-51 0.21370917506417442 1104 52-53 0.21590447164648907 1104 54-55 0.38333679289923595 1104 56-57 0.3864405657798926 1104 58-59 0.37522050814762054 1104 60-61 0.46048383176074736 1104 62-63 0.2599413503353105 1104 64-65 0.41094961818735243 1104 66-67 0.16522222517638596 1104 68-69 -0.08569409051694876 1104 70-71 0.02463139280422766 1104 72-73 0.11932133744851825 1104 74-75 -0.017497862078226234 1104 76-77 0.1367217959458138 1104 78-79 0.23686705400950459 1104 80-81 0.07559545893597885 1104 82-83 0.039375292276666585 1104 84-85 0.015559715538909558 1104 86-87 0.29611039076650414 1104 88-89 0.20812274301036027 1104 90-91 -0.13865316536750782 1104 92-93 -0.018186198677028642 1104 94-95 0.2552552611272567 1104 96-97 0.42015387539527893 1104 98-99 0.3841701905109254 1104 100-101 0.7363215898963524 1105 1 0.032421157992846616 1105 2 0.15145269728182953 1105 3 -0.030886621742901355 1105 4 -0.03583840251090464 1105 5 0.11201233911761932 1105 6 0.1467174971380416 1105 7 0.04118441563279873 1105 8 0.07552175310306097 1105 9 0.13609670141503472 1105 10-11 0.12015779988951181 1105 12-13 0.2264602686128825 1105 14-15 0.2457208173344938 1105 16-17 0.29237694332500297 1105 18-19 0.22614868797295884 1105 20-21 0.07343767847846294 1105 22-23 0.2429106685125646 1105 24-25 0.18623810144541153 1105 26-27 0.2894016569160627 1105 28-29 0.22225467543668032 1105 30-31 0.3337943145963891 1105 32-33 0.1998345348498134 1105 34-35 0.14509538434976577 1105 36-37 0.02932202199200873 1105 38-39 0.19645268722303655 1105 40-41 0.446754642989859 1105 42-43 0.4902821760066658 1105 44-45 0.6091021690242044 1105 46-47 0.490237178994569 1105 48-49 0.5818898251166189 1105 50-51 0.39423950227500626 1105 52-53 0.6597698731882389 1105 54-55 0.6486778833440567 1105 56-57 0.6377613058973068 1105 58-59 0.6662026242334633 1105 60-61 0.9281114323758928 1105 62-63 0.7707476256644128 1105 64-65 0.8683261282609607 1105 66-67 0.8595963849032486 1105 68-69 0.9280496346375635 1105 70-71 0.8153344656649786 1105 72-73 0.8705231093971761 1105 74-75 1.106913825818939 1105 76-77 1.1369742405965972 1105 78-79 1.0450405540917203 1105 80-81 1.028640753889988 1105 82-83 0.9750551034751531 1105 84-85 1.0801889314129376 1105 86-87 1.2744577004416122 1105 88-89 1.1951000810250392 1105 90-91 1.2808672865180881 1105 92-93 1.3061737429028284 1105 94-95 1.2831431408823555 1105 96-97 1.2176598963452854 1105 98-99 1.2466792582067185 1105 100-101 1.3463386129839172 1106 1 0.28767136800124504 1106 2 0.2561656130711931 1106 3 0.3012081165920897 1106 4 0.2506468750820119 1106 5 0.30006204292395466 1106 6 0.3530919339336904 1106 7 0.29228027764909825 1106 8 0.31628956563374544 1106 9 0.35185878644389135 1106 10-11 0.31614254837035816 1106 12-13 0.27324468544409797 1106 14-15 0.20278964554216827 1106 16-17 0.3383567825185736 1106 18-19 0.3361069044834366 1106 20-21 0.26055679960694533 1106 22-23 0.11863969767373561 1106 24-25 0.17667862815739 1106 26-27 0.0984745652869492 1106 28-29 0.10083545503150049 1106 30-31 0.23731318262383638 1106 32-33 0.3562738833328396 1106 34-35 0.38104227677091274 1106 36-37 0.22291322018539006 1106 38-39 0.19698289024933757 1106 40-41 0.2369437050977936 1106 42-43 0.26837693616070624 1106 44-45 0.14601346365780898 1106 46-47 0.28672322025440167 1106 48-49 0.15008637116027046 1106 50-51 0.18075150820978791 1106 52-53 0.19676026189470264 1106 54-55 0.29060046933840766 1106 56-57 0.26337796692738635 1106 58-59 0.3408565194801838 1106 60-61 0.3131566523665157 1106 62-63 0.2654155942013361 1106 64-65 0.10380936577228539 1106 66-67 -0.08640472786853337 1106 68-69 0.2320310666221168 1106 70-71 0.3134225710073686 1106 72-73 0.03565516922502354 1106 74-75 0.11437757891451383 1106 76-77 0.07194036651435809 1106 78-79 0.04414324547210313 1106 80-81 0.26612698061178364 1106 82-83 0.10468483411892393 1106 84-85 0.27739881980847514 1106 86-87 0.5492310550121609 1106 88-89 0.4001042811930482 1106 90-91 0.39654264080498436 1106 92-93 0.5562528369756841 1106 94-95 0.6796051811821471 1106 96-97 0.7039444386360678 1106 98-99 0.7875256193338878 1106 100-101 0.8977486693861714 1107 1 0.2797803699687371 1107 2 0.2854368260427087 1107 3 0.2973434464197702 1107 4 0.1885782086790826 1107 5 0.2345013076049014 1107 6 0.39106506195738433 1107 7 0.25002847805731676 1107 8 0.28258722343727527 1107 9 0.275304671204303 1107 10-11 0.2680086432331876 1107 12-13 0.41824834574214975 1107 14-15 0.33055211914136606 1107 16-17 0.5436600746839559 1107 18-19 0.4312697253180815 1107 20-21 0.5840394187769178 1107 22-23 0.5550356118085347 1107 24-25 0.5231337247774235 1107 26-27 0.5175645639332203 1107 28-29 0.5937468311327123 1107 30-31 0.5875632920384959 1107 32-33 0.46239131252874444 1107 34-35 0.3953719822253561 1107 36-37 0.5293458818726506 1107 38-39 0.5631286818265764 1107 40-41 0.7497011909658937 1107 42-43 0.9288665486685446 1107 44-45 0.8468912123313643 1107 46-47 0.5920502353569148 1107 48-49 0.6429340041894349 1107 50-51 0.5082814838822785 1107 52-53 0.6326288171086887 1107 54-55 0.5906282665749814 1107 56-57 0.7040302096978124 1107 58-59 0.5877071517194565 1107 60-61 0.6401244404293394 1107 62-63 0.6180826831932364 1107 64-65 0.5567761786318215 1107 66-67 0.5399514552751654 1107 68-69 0.5255508276316014 1107 70-71 0.25887197280821184 1107 72-73 0.4111600209270918 1107 74-75 0.6799724857773448 1107 76-77 0.6314826386854513 1107 78-79 0.690990854844042 1107 80-81 0.7701713799614716 1107 82-83 1.0215641967407727 1107 84-85 0.82649182817919 1107 86-87 0.7621483585619266 1107 88-89 0.7092177662042225 1107 90-91 0.8008892611735909 1107 92-93 1.0566185382086175 1107 94-95 1.0806466904078107 1107 96-97 0.820048830073727 1107 98-99 0.8254077327650151 1107 100-101 0.968709165767276 1108 1 -0.09803146864880574 1108 2 -0.1298047647683127 1108 3 -0.1128433844923471 1108 4 -0.024383089531205826 1108 5 -0.11405366804594763 1108 6 -0.1476880972675545 1108 7 -0.13086167851695762 1108 8 -0.15185757488602292 1108 9 -0.24304580242259277 1108 10-11 -0.33453902767317345 1108 12-13 -0.3050093288628162 1108 14-15 -0.17433344243013238 1108 16-17 -0.14407943566186532 1108 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-1.7016944848792797 2316 70-71 -1.4528235127202755 2316 72-73 -1.7863824850084171 2316 74-75 -2.015408170366694 2316 76-77 -2.074373502569326 2316 78-79 -2.451542408400332 2316 80-81 -2.222145959396723 2316 82-83 -2.6541565939183656 2316 84-85 -2.215480172210711 2316 86-87 -2.6961080973969125 2316 88-89 -2.9115512222171738 2316 90-91 -3.423066279915478 2316 92-93 -3.442515068285985 2316 94-95 -3.6881537440508936 2316 96-97 -4.2244570775898715 2316 98-99 -4.585067404221764 2316 100-101 -4.762513898141325 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1706.0 3 582.0 4 527.0 5 591.0 6 801.0 7 911.0 8 1281.0 9 1601.0 10 2121.0 11 3252.0 12 5168.0 13 7193.0 14 8318.0 15 11364.0 16 15894.0 17 22227.0 18 18525.0 19 8631.0 20 7687.0 21 7658.0 22 7817.0 23 8246.0 24 9153.0 25 10229.0 26 11745.0 27 13982.0 28 16609.0 29 20109.0 30 24533.0 31 30227.0 32 39610.0 33 51230.0 34 71761.0 35 104939.0 36 150789.0 37 216540.0 38 198109.0 39 36720.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.619755374964825 19.22094914374269 15.18954041121606 19.969755070076424 2 15.188969313717804 21.48579790430897 41.030163665978556 22.29506911599467 3 18.042452008500852 28.63672786816709 31.358742988537784 21.96207713479427 4 11.63657842602579 17.695230893342835 38.670954117538415 31.997236563092958 5 11.979123085934232 39.06419964856497 33.33263604161437 15.624041223886426 6 27.796443204602912 38.12649289512684 18.447476244442505 15.62958765582774 7 25.744595394028945 36.402557653051 21.17861489791953 16.67423205500052 8 26.77944387068098 32.93547557728594 20.215618858370483 20.069461693662603 9 28.468377087265612 15.692682000149885 20.916139894070344 34.92280101851416 10-11 23.822673645997355 27.99824674379716 26.811095358140918 21.367984252064566 12-13 26.44459906752696 26.228015407894866 27.891409283862274 19.4359762407159 14-15 23.276650767898314 25.827758308515108 28.744299359227938 22.151291564358644 16-17 19.659002796630105 29.71114557287356 28.784554935050227 21.84529669544611 18-19 19.595847383049726 26.33352501938643 34.339336591996236 19.73129100556761 20-21 21.487372169765088 25.06555145337123 34.412999204974895 19.034077171888782 22-23 20.73610830435601 25.48143592816875 34.3484108346167 19.434044932858548 24-25 19.91813082956434 25.781517039821196 34.77323563490408 19.52711649571038 26-27 19.816774762097168 25.931234586822345 34.512107617070484 19.739883034010003 28-29 19.56326842868159 25.803223911687063 34.79209509847164 19.841412561159704 30-31 19.680714419447117 25.956311038929936 34.712651685686794 19.65032285593615 32-33 19.440777270566024 26.163214975538306 34.32130771226986 20.07470004162581 34-35 19.76291451860109 26.10809704611955 34.217160721750034 19.911827713529327 36-37 20.14556702431429 25.846506731038016 33.811415986770434 20.19651025787726 38-39 19.813046757033064 26.146981526731118 33.910735464949205 20.129236251286613 40-41 19.80213798265387 26.112761581973054 34.00216227890089 20.082938156472185 42-43 19.863771180167927 26.478497198618555 33.227457892197755 20.430273729015763 44-45 19.929491580918576 26.76367923850559 32.88877703987422 20.418052140701608 46-47 20.287066007069107 26.984779388650242 31.97638928679345 20.7517653174872 48-49 20.429243299114503 27.316084302150916 31.110604122456337 21.144068276278244 50-51 20.640132291626756 27.81372238264256 30.50966934059695 21.036475985133738 52-53 20.681621058644854 28.063302030067856 30.363761129146873 20.891315782140417 54-55 20.376698758561833 28.059158817868695 30.316956451690157 21.247185971879315 56-57 20.536206080508855 28.479149984520713 29.717101284193227 21.26754265077721 58-59 20.658524765541898 27.86331916650085 30.094932726776356 21.383223341180894 60-61 20.648068364934016 27.85552347617246 30.544604009260112 20.951804149633414 62-63 20.771147311421558 27.77827609809963 30.656830694364373 20.793745896114437 64-65 20.630207938751287 27.961444381456452 30.556302993729357 20.852044686062904 66-67 20.569563751930232 28.257097990989195 30.116131920426902 21.05720633665367 68-69 20.675163929927635 28.186190596907917 30.193510056198324 20.945135416966128 70-71 20.56427512202262 28.350285518123474 30.023358989595188 21.06208037025872 72-73 20.590266318228643 28.423460177661592 29.6234291770111 21.362844327098664 74-75 20.48145082313782 28.695890341765377 29.503722376102594 21.318936458994205 76-77 20.488885927633003 28.64087499885687 29.706404543263293 21.163834530246834 78-79 20.38200610910155 28.596413450475254 29.59012231267904 21.43145812774415 80-81 20.20526294003978 29.03178721670686 29.355868250036494 21.407081593216866 82-83 20.297123704569568 28.798753390460895 29.20826532087653 21.695857584093012 84-85 20.318886609627558 28.632302411483646 29.151036357532355 21.89777462135644 86-87 20.43462847357414 28.59800132571353 29.038221836644567 21.929148364067764 88-89 20.473656448147246 28.420911412540907 29.03947601684642 22.065956122465423 90-91 20.08288760736624 28.679983274387162 29.277531926755756 21.959597191490847 92-93 20.110085205618116 28.58046969803882 29.229116086447853 22.080329009895213 94-95 19.925731850002375 28.49016978998434 29.180947188883465 22.403151171129824 96-97 20.199943652085157 28.45547926862671 28.865329789888467 22.479247289399666 98-99 20.02131576735238 28.397053424003015 28.986264091642994 22.59536671700161 100-101 20.05580667396929 28.42828920824064 29.149967800490266 22.36593631729981 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1355.0 1 999.0 2 840.0 3 1056.5 4 925.0 5 967.5 6 1736.0 7 3583.5 8 5990.0 9 6884.5 10 5511.5 11 4208.0 12 4185.5 13 4305.5 14 4376.0 15 4403.0 16 4394.0 17 4589.5 18 4627.5 19 4720.0 20 5080.5 21 5519.0 22 6182.0 23 7053.0 24 8053.5 25 9303.0 26 10778.0 27 12227.5 28 13215.0 29 14561.0 30 16986.0 31 19203.5 32 22082.5 33 24725.5 34 27185.0 35 29426.5 36 32058.5 37 35067.0 38 35307.0 39 34469.5 40 33919.0 41 34062.5 42 34041.0 43 34883.0 44 36446.0 45 36736.5 46 35951.0 47 35503.0 48 38145.5 49 37892.0 50 34163.0 51 31788.0 52 30201.0 53 30125.5 54 29731.5 55 27631.5 56 25040.5 57 22690.0 58 21752.5 59 20451.0 60 17798.5 61 15528.5 62 13180.5 63 9747.0 64 6510.0 65 4780.5 66 3399.5 67 2582.5 68 1867.5 69 1107.0 70 797.5 71 596.0 72 477.0 73 431.5 74 324.0 75 236.5 76 168.0 77 86.5 78 46.0 79 29.5 80 17.0 81 12.0 82 8.5 83 8.0 84 8.5 85 5.5 86 3.5 87 3.0 88 4.5 89 5.0 90 3.0 91 1.5 92 0.5 93 1.5 94 2.0 95 0.5 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.037965964731708636 2 0.02847447354878148 3 0.024120578510741494 4 0.04797992331920059 5 0.09587276873764038 6 0.11294003728675711 7 0.05451076587626057 8 0.08725205656232123 9 0.07340667034135408 10-11 0.07122972282233409 12-13 0.06996709326130249 14-15 0.06260901064701492 16-17 0.05111472774658938 18-19 0.059822517822669334 20-21 0.05525092803272736 22-23 0.03248005698377826 24-25 0.008838406927221161 26-27 0.002525259122063189 28-29 0.006226069904397173 30-31 0.0045280508395615805 32-33 0.006182530954016773 34-35 0.00670499835858157 36-37 0.005224674045647977 38-39 0.0030477265266279866 40-41 0.006226069904397173 42-43 0.0030477265266279866 44-45 0.002046330667878791 46-47 0.0017415580152159923 48-49 3.4831160304319846E-4 50-51 0.0021769475190199904 52-53 0.005311751946408777 54-55 0.01149428290042555 56-57 0.007706394217330766 58-59 0.005006979293745978 60-61 0.0026558759732043884 62-63 0.008272400572275964 64-65 0.0026123370228239885 66-67 0.019157138167375916 68-69 0.04336479457887821 70-71 0.05738433660136694 72-73 0.0029606486258671873 74-75 0.012060289255370747 76-77 0.02050684562916831 78-79 0.023859344808459096 80-81 0.07353728719249528 82-83 0.02812616194573828 84-85 0.010492887041676354 86-87 0.02925817465562867 88-89 0.043321255628497805 90-91 0.039794600647685426 92-93 0.04075245755605422 94-95 0.010928276545480353 96-97 0.015108015781998732 98-99 0.03661625726991624 100-101 0.07523530625733087 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1148397.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.49871816284703 #Duplication Level Percentage of deduplicated Percentage of total 1 75.27556768941518 37.26044109606676 2 13.218497787847081 13.085973930737193 3 4.60419451537507 6.8370518005043 4 2.1480663878592083 4.253061309109111 5 1.1917996270793705 2.949627692369398 6 0.7238485854487186 2.1497746274220972 7 0.5026352751494149 1.741586127632817 8 0.35807501552593635 1.4179403419740317 9 0.27476712952828913 1.224055863444174 >10 1.5018029460019247 14.050819519106048 >50 0.13208683835234472 4.492424176014345 >100 0.06478517668827072 5.595836047532143 >500 0.002112559488700168 0.7786903878748515 >1k 0.0012323263684084313 0.9756286462227883 >5k 1.7604662405834733E-4 0.7224845575056393 >10k+ 3.5209324811669466E-4 2.4646038764843805 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16509 1.437569063660041 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11774 1.0252552035576548 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8291 0.7219628752077896 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2417 0.21046728613885266 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2218 0.19313878388745354 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1630 0.14193697824010337 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1578 0.1374089274005418 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1199 0.10440640301219875 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 8.707790076079961E-5 0.0 0.0 0.0 0.0 10-11 8.707790076079961E-5 0.0 0.0 0.0 0.0 12-13 8.707790076079961E-5 0.0 0.0 0.0 0.0 14-15 8.707790076079961E-5 0.0 0.0 0.0 0.0 16-17 8.707790076079961E-5 0.0 0.0 0.0 0.0 18-19 8.707790076079961E-5 0.0 0.0 0.0 0.0 20-21 8.707790076079961E-5 0.0 0.0 1.7415580152159923E-4 0.0 22-23 8.707790076079961E-5 0.0 0.0 6.966232060863969E-4 0.0 24-25 8.707790076079961E-5 0.0 0.0 0.001393246412172794 0.0 26-27 8.707790076079961E-5 0.0 0.0 0.002133408568639591 0.0 28-29 8.707790076079961E-5 0.0 0.0 0.004179739236518381 0.0 30-31 8.707790076079961E-5 0.0 0.0 0.008881945877601561 0.0 32-33 8.707790076079961E-5 0.0 0.0 0.013235840915641542 0.0 34-35 8.707790076079961E-5 0.0 0.0 0.018982982365854315 0.0 36-37 8.707790076079961E-5 0.0 0.0 0.026253987079381085 0.0 38-39 8.707790076079961E-5 0.0 0.0 0.037530575227904636 0.0 40-41 8.707790076079961E-5 0.0 0.0 0.05799388190669255 0.0 42-43 8.707790076079961E-5 0.0 0.0 0.07950212339461005 0.0 44-45 8.707790076079961E-5 0.0 0.0 0.10549487677170874 0.0 46-47 8.707790076079961E-5 0.0 0.0 0.1560000592129725 0.0 48-49 8.707790076079961E-5 0.0 0.0 0.19135368692185717 0.0 50-51 8.707790076079961E-5 0.0 0.0 0.2361117279129082 0.0 52-53 8.707790076079961E-5 0.0 0.0 0.28992587058308233 0.0 54-55 8.707790076079961E-5 0.0 0.0 0.35436351714607406 0.0 56-57 8.707790076079961E-5 0.0 0.0 0.47283300113114196 0.0 58-59 8.707790076079961E-5 0.0 0.0 0.5782408000020899 0.0 60-61 8.707790076079961E-5 0.0 0.0 0.675289120400001 0.0 62-63 8.707790076079961E-5 0.0 0.0 0.7796084455114389 0.0 64-65 8.707790076079961E-5 0.0 0.0 0.8811848167489118 0.0 66-67 8.707790076079961E-5 0.0 0.0 1.0293914038437928 0.0 68-69 1.3061685114119942E-4 0.0 0.0 1.2120808396399503 0.0 70-71 1.7415580152159923E-4 0.0 0.0 1.3410432106666945 0.0 72-73 1.7415580152159923E-4 0.0 0.0 1.490512427322607 0.0 74-75 2.6123370228239883E-4 0.0 0.0 1.6956244225646704 0.0 76-77 3.4831160304319846E-4 0.0 0.0 1.9236814446572048 0.0 78-79 3.4831160304319846E-4 0.0 0.0 2.166498170928694 0.0 80-81 3.4831160304319846E-4 0.0 0.0 2.41323340273442 0.0 82-83 3.4831160304319846E-4 0.0 0.0 2.7072954736036405 0.0 84-85 3.4831160304319846E-4 0.0 0.0 3.0694960018181865 0.0 86-87 3.4831160304319846E-4 0.0 0.0 3.3867207942897797 0.0 88-89 3.4831160304319846E-4 0.0 0.0 3.7850151123696767 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6050 0.0 49.64943 1 GTATCAA 11505 0.0 44.32673 1 ATCAACG 14905 0.0 34.1545 3 TATCAAC 14970 0.0 34.0062 2 TCAACGC 15050 0.0 33.795326 4 CAACGCA 15305 0.0 33.13908 5 AACGCAG 15485 0.0 32.718887 6 GCGTATC 80 4.4779724E-4 29.705208 1 ACGCAGA 17350 0.0 29.140705 7 CGCAGAG 17400 0.0 28.867062 8 GCAGAGT 17945 0.0 27.354809 9 GTACATG 10140 0.0 25.779669 1 TACATGG 10300 0.0 25.058382 2 GAGTACT 10505 0.0 24.700815 12-13 AGTACTT 10765 0.0 24.236673 12-13 ACATGGG 10185 0.0 23.941246 3 ATGGGAG 2490 0.0 23.290018 5 GTACTTT 11090 0.0 23.22491 14-15 TATTCCG 125 2.2448221E-4 22.81658 5 CGTATCA 125 2.245485E-4 22.815588 2 >>END_MODULE