FastQCFastQC Report
Thu 26 May 2016
SRR936964_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936964_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1148397
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168971.4713552891552313No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132381.1527372502714655No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98650.8590234910052883No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28750.2503489646872989No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27060.23563279945872379No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24250.2111639093449391No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19370.16866989377366887No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13520.11772932182860109No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13450.11711977652327549No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12150.10579964942437155No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71450.046.5434231
GTATCAA125850.040.9957731
TATCAAC167350.030.7389892
ATCAACG167200.030.7097513
TCAACGC168700.030.4366934
CAACGCA171200.029.9922315
AACGCAG174650.029.3764116
CATATAG3250.029.2303123
TGAACCG1257.3917854E-626.5995865
ACGCAGA195600.026.1328647
CGCAGAG195850.026.0521288
GCAGAGT200550.025.1099419
AGTACTT124450.023.7994112-13
GAGTACT122000.023.63488812-13
GTACATG96900.022.6506521
GTACTTT130800.021.8789614-15
TACATGG99700.021.581912
AGAGTAC192600.021.0774610-11
TACTTTT139650.021.0706414-15
ACGCAGT1604.98319E-520.7845468
Produced by FastQC (version 0.11.5)