##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936964_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1148397 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.986401915017193 31.0 31.0 34.0 30.0 34.0 2 32.09148578409731 33.0 31.0 34.0 30.0 34.0 3 32.177456053960434 33.0 31.0 34.0 30.0 34.0 4 35.93043956053525 37.0 35.0 37.0 35.0 37.0 5 35.77553842442988 37.0 35.0 37.0 35.0 37.0 6 35.89184141024402 37.0 35.0 37.0 35.0 37.0 7 35.84268332292753 37.0 35.0 37.0 35.0 37.0 8 35.80148415574057 37.0 35.0 37.0 35.0 37.0 9 37.43533029083148 39.0 37.0 39.0 34.0 39.0 10-11 37.34722530623121 39.0 37.0 39.0 34.0 39.0 12-13 37.22690062757043 39.0 37.0 39.0 33.5 39.0 14-15 38.25360219505972 40.0 37.5 41.0 33.0 41.0 16-17 38.27359310412688 40.0 37.5 41.0 33.5 41.0 18-19 38.50775472245225 40.0 38.0 41.0 34.0 41.0 20-21 38.552323369009144 40.0 38.0 41.0 34.0 41.0 22-23 38.44593550836514 40.0 38.0 41.0 34.0 41.0 24-25 38.32596393059195 40.0 38.0 41.0 34.0 41.0 26-27 38.077667827415084 40.0 37.0 41.0 33.0 41.0 28-29 37.88166722831913 40.0 37.0 41.0 33.0 41.0 30-31 37.67672068108851 39.0 36.5 41.0 33.0 41.0 32-33 37.36843704746703 39.0 35.5 41.0 33.0 41.0 34-35 37.15488067279869 39.0 35.0 41.0 32.5 41.0 36-37 36.91478904943151 39.0 35.0 41.0 32.0 41.0 38-39 36.66359673527535 39.0 35.0 41.0 31.0 41.0 40-41 36.214980533735286 39.0 35.0 40.5 30.5 41.0 42-43 35.85604499140976 39.0 35.0 40.0 30.0 41.0 44-45 35.38399569138547 38.0 35.0 40.0 28.0 41.0 46-47 34.67688395215244 38.0 34.5 40.0 24.0 41.0 48-49 34.02487510852083 38.0 34.0 40.0 19.5 41.0 50-51 33.639709960928144 38.0 33.0 40.0 16.0 41.0 52-53 33.33051549246471 38.0 33.0 40.0 12.0 41.0 54-55 32.90990136686181 37.5 33.0 40.0 8.0 41.0 56-57 32.68974927660034 37.0 33.0 40.0 7.5 41.0 58-59 32.35708208920783 37.0 32.0 40.0 4.0 41.0 60-61 31.962553019556825 36.0 31.0 39.0 2.0 40.5 62-63 31.567981281734454 35.5 31.0 39.0 2.0 40.0 64-65 31.238090573207696 35.0 31.0 39.0 2.0 40.0 66-67 30.903969620261982 35.0 30.5 38.0 2.0 40.0 68-69 30.452704944370282 35.0 30.0 38.0 2.0 40.0 70-71 30.013513184029563 34.5 29.0 37.0 2.0 39.5 72-73 29.6035939661981 34.0 29.0 37.0 2.0 39.0 74-75 29.12112187684224 34.0 28.5 36.0 2.0 39.0 76-77 27.74148791750588 32.5 26.5 35.0 2.0 37.0 78-79 28.387342965890717 34.0 28.0 35.0 2.0 37.0 80-81 28.25443640134901 34.0 28.0 35.0 2.0 37.0 82-83 27.898222914201273 34.0 27.0 35.0 2.0 36.5 84-85 27.44344986968792 33.0 26.0 35.0 2.0 36.0 86-87 27.029192866230055 33.0 26.0 35.0 2.0 36.0 88-89 26.617019201547897 33.0 25.0 35.0 2.0 35.0 90-91 26.127137653616302 32.5 24.5 34.5 2.0 35.0 92-93 25.60981219909143 32.0 22.5 34.0 2.0 35.0 94-95 25.025596113539134 32.0 19.0 34.0 2.0 35.0 96-97 24.213251602015678 31.0 8.5 34.0 2.0 35.0 98-99 23.310224164639926 31.0 2.0 34.0 2.0 35.0 100-101 20.328291522879283 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1816121168549678 1101 2 0.2804701212053118 1101 3 0.2265054780108855 1101 4 0.09712700027935028 1101 5 0.04651177963149422 1101 6 0.04769137796851908 1101 7 -0.058056645734922085 1101 8 0.10225763361079032 1101 9 0.10334251262273142 1101 10-11 0.18739707890369317 1101 12-13 0.2540424427371377 1101 14-15 0.27114059718675065 1101 16-17 0.2861499054810892 1101 18-19 0.08514417317940115 1101 20-21 0.07341064822219323 1101 22-23 0.06885459131996896 1101 24-25 0.053819598222347054 1101 26-27 0.07000345458445167 1101 28-29 0.3367723115195602 1101 30-31 0.2937727220390869 1101 32-33 0.38029665131105617 1101 34-35 0.21039601965146915 1101 36-37 0.4362213189388129 1101 38-39 0.399044203148911 1101 40-41 0.5796687736627817 1101 42-43 0.6003976536280504 1101 44-45 0.13645766555110583 1101 46-47 0.2805813535714279 1101 48-49 0.5790824860843458 1101 50-51 0.5844463817499488 1101 52-53 0.6006413817336451 1101 54-55 0.3126390355011708 1101 56-57 0.5854785195809669 1101 58-59 0.565262683790948 1101 60-61 0.6513140299152553 1101 62-63 0.5097996962590834 1101 64-65 0.5595797016064488 1101 66-67 0.6630479910564162 1101 68-69 0.17867133824883297 1101 70-71 0.3068244365170685 1101 72-73 0.4506559841933928 1101 74-75 0.43255724075692825 1101 76-77 0.4606337880181073 1101 78-79 0.4377162730783688 1101 80-81 0.46516954687900736 1101 82-83 0.6582267153358003 1101 84-85 0.31957784928911437 1101 86-87 -0.024855440357971048 1101 88-89 0.021488339251096278 1101 90-91 0.25317017528219665 1101 92-93 -0.00926915480070889 1101 94-95 -0.04419861709707007 1101 96-97 -0.36358377561406385 1101 98-99 -0.13598207956608377 1101 100-101 0.2040542862822683 1102 1 -0.13496850663762672 1102 2 -0.021639443644694722 1102 3 0.039148176195141104 1102 4 0.04599045530918744 1102 5 -0.1353931660906369 1102 6 0.061646228225022526 1102 7 0.09310933172515945 1102 8 0.10934625377412743 1102 9 0.16505398404965632 1102 10-11 0.23280720621314543 1102 12-13 0.21497093901045616 1102 14-15 0.11870005613551626 1102 16-17 0.1618753960943522 1102 18-19 0.18917358743232882 1102 20-21 0.18898636241600286 1102 22-23 0.10976296610448344 1102 24-25 0.1534498430692537 1102 26-27 0.33952831490677227 1102 28-29 0.15876425785820913 1102 30-31 -6.593195574779998E-4 1102 32-33 -0.05200102311697208 1102 34-35 0.045449456591228454 1102 36-37 0.22529472235287784 1102 38-39 0.3578782307546575 1102 40-41 0.3114288618893326 1102 42-43 0.3706861882801249 1102 44-45 0.38950615722464477 1102 46-47 0.35623526396393146 1102 48-49 0.38538276131105675 1102 50-51 0.21930442950960582 1102 52-53 0.3405027501468538 1102 54-55 0.578970141387714 1102 56-57 0.6381507718351358 1102 58-59 0.4297351861959555 1102 60-61 0.42239790035353053 1102 62-63 0.3833592899590137 1102 64-65 0.47184453930475456 1102 66-67 0.48298295589193785 1102 68-69 0.3616502405739972 1102 70-71 0.2563537952991233 1102 72-73 0.25993091657718637 1102 74-75 0.15426038395434816 1102 76-77 0.2904871473492996 1102 78-79 -0.033741810446070275 1102 80-81 0.22172486146977022 1102 82-83 0.39772562339548756 1102 84-85 0.37374654862128764 1102 86-87 0.2139112015367708 1102 88-89 0.36466990529259036 1102 90-91 0.3638758508895492 1102 92-93 0.5625932328581094 1102 94-95 0.6251610464100352 1102 96-97 1.0463573644719233 1102 98-99 0.6785106608418872 1102 100-101 0.7740445953436783 1103 1 0.08446808379103388 1103 2 0.2011273130728739 1103 3 0.20602327635769058 1103 4 0.06382129697986727 1103 5 0.030680633675842728 1103 6 0.06395712133154774 1103 7 0.11511027420705489 1103 8 0.1706333329960259 1103 9 0.2343721918840913 1103 10-11 0.12174768676444359 1103 12-13 0.19616255855303422 1103 14-15 0.16818202496875045 1103 16-17 0.23398742361329994 1103 18-19 0.2655236832068013 1103 20-21 0.1546537407137265 1103 22-23 0.032242637876585434 1103 24-25 0.287352385862512 1103 26-27 0.22286517777756387 1103 28-29 0.19444525826266812 1103 30-31 0.2003702560585694 1103 32-33 0.14845476417974623 1103 34-35 0.09265034260051408 1103 36-37 -0.058655612285427594 1103 38-39 -0.0932481686592439 1103 40-41 -0.03505342937191358 1103 42-43 -0.2913379442168065 1103 44-45 -0.2204439427471101 1103 46-47 -0.3414462591936527 1103 48-49 -0.09911756111696235 1103 50-51 -0.23760468776261945 1103 52-53 -0.16083155810195393 1103 54-55 -0.1272411767895889 1103 56-57 -0.36342750376640964 1103 58-59 -0.37273019571907895 1103 60-61 -0.5012167084974024 1103 62-63 -0.024973852588512813 1103 64-65 -0.18847468351017582 1103 66-67 -0.057290735889775846 1103 68-69 -0.19531196284946262 1103 70-71 -0.2106774630477588 1103 72-73 -0.02386106583999137 1103 74-75 -0.2914820399411937 1103 76-77 -0.01753200945373834 1103 78-79 -0.04777148336287951 1103 80-81 -0.23343427297317376 1103 82-83 -0.04309261878038484 1103 84-85 -0.39231213136985943 1103 86-87 -0.47846504524848754 1103 88-89 -0.09849436319646188 1103 90-91 -0.34623894493147844 1103 92-93 -0.45364860150928266 1103 94-95 -0.10181659158534018 1103 96-97 -0.12741821990500668 1103 98-99 -0.29635140101850155 1103 100-101 0.44406789983019834 1104 1 0.016346346406475476 1104 2 0.07781996912070355 1104 3 -0.01609391009660044 1104 4 -0.015106111220731577 1104 5 -0.1986198838809301 1104 6 -0.025803843287199868 1104 7 0.02230977895876407 1104 8 0.045242934566545046 1104 9 -0.004384333346806102 1104 10-11 0.08774537783659042 1104 12-13 0.15758422231959202 1104 14-15 0.2425710514266015 1104 16-17 0.13913873089033046 1104 18-19 0.18377704063329503 1104 20-21 0.21263766745268242 1104 22-23 0.13302259816747863 1104 24-25 -0.023457746649874878 1104 26-27 0.12037722435766796 1104 28-29 0.03636596276452764 1104 30-31 0.04351653277297629 1104 32-33 0.17072420602153215 1104 34-35 0.17889214423350097 1104 36-37 0.07636647311144173 1104 38-39 0.10304416168475683 1104 40-41 0.21558236235609485 1104 42-43 0.08843746514384065 1104 44-45 0.44563294343501525 1104 46-47 0.46154056764792273 1104 48-49 0.5627848504299067 1104 50-51 0.6010157557300033 1104 52-53 0.4949973693062404 1104 54-55 0.5798177845274992 1104 56-57 0.4874653932130144 1104 58-59 0.20231135382806542 1104 60-61 0.3717560082294966 1104 62-63 0.6992486968247711 1104 64-65 0.5923556381781303 1104 66-67 0.3380978220899671 1104 68-69 0.39061612575982707 1104 70-71 0.45822562890780105 1104 72-73 0.49042751667908036 1104 74-75 0.45443550791050313 1104 76-77 0.4906175577617162 1104 78-79 0.6299448531382588 1104 80-81 0.5500925864387725 1104 82-83 0.6869635985934615 1104 84-85 0.8070490094858904 1104 86-87 0.7878605805044252 1104 88-89 0.7602441479354702 1104 90-91 0.8698103345045602 1104 92-93 0.8077635811525674 1104 94-95 0.8104650641501685 1104 96-97 0.7825796050180287 1104 98-99 0.5464964997533741 1104 100-101 1.0119930437199045 1105 1 -0.002199146827813081 1105 2 0.1583111238857171 1105 3 0.039913982425815675 1105 4 0.057136583843536926 1105 5 0.02773398355176937 1105 6 0.05986000876669806 1105 7 0.09175043512141201 1105 8 0.16210449624138334 1105 9 0.17909359950871817 1105 10-11 0.22476624079106244 1105 12-13 0.12685706565883237 1105 14-15 0.25524261223094413 1105 16-17 0.08658865302904672 1105 18-19 -0.11347943732857857 1105 20-21 0.13469030361319767 1105 22-23 0.03599244989251815 1105 24-25 -0.05492240923486946 1105 26-27 -0.047605346322001196 1105 28-29 -0.05956397175462058 1105 30-31 0.15974408115820182 1105 32-33 0.17517293847856763 1105 34-35 0.3287630202454537 1105 36-37 0.3831031665701232 1105 38-39 0.37036563689004964 1105 40-41 0.3149352915845469 1105 42-43 0.44937141362964894 1105 44-45 0.5283994853301834 1105 46-47 0.5071368109620025 1105 48-49 0.5240331457031004 1105 50-51 0.6543270364271692 1105 52-53 0.6064521662382134 1105 54-55 0.9541211436856329 1105 56-57 0.8220817450492817 1105 58-59 0.7282618901520408 1105 60-61 0.6872194521161816 1105 62-63 0.8533327870084442 1105 64-65 0.6925389265356934 1105 66-67 0.7232195468382479 1105 68-69 0.5715647760030826 1105 70-71 0.4960918429150958 1105 72-73 0.5908468069323973 1105 74-75 0.6370163433958993 1105 76-77 0.7779972596642395 1105 78-79 0.814661312336046 1105 80-81 0.9070057977088304 1105 82-83 0.8083285407052898 1105 84-85 0.5840784807062356 1105 86-87 0.7795802025145058 1105 88-89 1.027191308658562 1105 90-91 1.0851233755437448 1105 92-93 1.0997665988817253 1105 94-95 1.1803385516585116 1105 96-97 1.2410582632892186 1105 98-99 1.503614761850887 1105 100-101 1.5750660011912032 1106 1 -0.0938959056072548 1106 2 0.03690663134237582 1106 3 0.01856731037711512 1106 4 0.0712022373787704 1106 5 -0.09251082624061979 1106 6 0.06418672728998587 1106 7 0.1489294495547142 1106 8 0.1266768973192427 1106 9 0.1737184754128478 1106 10-11 0.1111527871620055 1106 12-13 0.19735934029527158 1106 14-15 0.25932877567748136 1106 16-17 0.18905857000753912 1106 18-19 0.13996906424784328 1106 20-21 0.1075179439915388 1106 22-23 0.12573367590519524 1106 24-25 0.0752604344425194 1106 26-27 0.2576006801008788 1106 28-29 0.12424192593583427 1106 30-31 0.19619226635833087 1106 32-33 -0.07297135092937879 1106 34-35 -0.14385952101983435 1106 36-37 0.08260132831477307 1106 38-39 0.059763758269447464 1106 40-41 0.07187375094110138 1106 42-43 0.1975555228122019 1106 44-45 -0.025095927213584446 1106 46-47 -0.35421455218342857 1106 48-49 -0.4686138329033227 1106 50-51 -0.32144126703223463 1106 52-53 -0.378521596348655 1106 54-55 -0.08478713991843989 1106 56-57 -0.17340498351077116 1106 58-59 -0.07704123106371696 1106 60-61 -0.27057595060902173 1106 62-63 -0.21924775257676998 1106 64-65 -0.3175027478585548 1106 66-67 -0.24552411199901414 1106 68-69 -0.3246687096999814 1106 70-71 -0.2821027394136486 1106 72-73 -0.31844865579520487 1106 74-75 -0.1541420302117693 1106 76-77 0.16847869710355212 1106 78-79 0.11208720937192496 1106 80-81 -0.017609914192373566 1106 82-83 0.03595473666221949 1106 84-85 0.11598775707728848 1106 86-87 -0.034385628846024474 1106 88-89 0.2869820639251408 1106 90-91 0.05229933530941011 1106 92-93 0.40938934742802857 1106 94-95 0.44193592464434417 1106 96-97 0.3545577123580905 1106 98-99 0.37856430528717055 1106 100-101 0.962162212312375 1107 1 0.21144458096924623 1107 2 0.24128400610037914 1107 3 0.21630094845052383 1107 4 0.2019077351858769 1107 5 0.10433303740440891 1107 6 0.15239333895798524 1107 7 0.13881861546676078 1107 8 0.13475518169745726 1107 9 0.3184211632676224 1107 10-11 0.2526525499326411 1107 12-13 0.24183459024039422 1107 14-15 0.38011138288741364 1107 16-17 0.30278803915260966 1107 18-19 0.23198777448235575 1107 20-21 0.23178440580418425 1107 22-23 0.2337725972935587 1107 24-25 0.2523127313616911 1107 26-27 0.35583401128754133 1107 28-29 0.3613148966389943 1107 30-31 0.39801827537173295 1107 32-33 0.2227554318066325 1107 34-35 0.4711034094266182 1107 36-37 0.4578991793189573 1107 38-39 0.36541014587727005 1107 40-41 0.5317862586461288 1107 42-43 0.47118317389971054 1107 44-45 0.2989787796668537 1107 46-47 0.29209978396204406 1107 48-49 0.4235963036908572 1107 50-51 -0.07616942583003095 1107 52-53 0.19484536528853624 1107 54-55 0.2640129788933905 1107 56-57 0.11780228398801995 1107 58-59 0.029254284446878387 1107 60-61 0.2445285288860788 1107 62-63 -0.020732111866895053 1107 64-65 0.14660924543330722 1107 66-67 0.027653066141187566 1107 68-69 -0.08005828501340417 1107 70-71 -0.22211295144807863 1107 72-73 -0.04233403918020784 1107 74-75 -0.007339763849842029 1107 76-77 -0.2169529880661294 1107 78-79 -0.06966893197735047 1107 80-81 0.05540777483869519 1107 82-83 0.11050685943794747 1107 84-85 0.22435130813154913 1107 86-87 0.49901245518180204 1107 88-89 0.5803378672389918 1107 90-91 0.5269023211137629 1107 92-93 0.5785174604885235 1107 94-95 0.48767355027404946 1107 96-97 0.48025445042137704 1107 98-99 0.5730808896573691 1107 100-101 1.1030751445331184 1108 1 -0.04794897626666739 1108 2 0.01806935346101568 1108 3 -0.021799304287469567 1108 4 0.029727407653872717 1108 5 -0.15994190763021976 1108 6 -0.0913094010917348 1108 7 -0.07266837777301305 1108 8 -0.029594505975914842 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-1.4943360276414985 2316 90-91 -1.2653216339921762 2316 92-93 -1.206844971366209 2316 94-95 -1.602091518271557 2316 96-97 -1.4860475854075403 2316 98-99 -2.062580406617009 2316 100-101 -2.7917331087897246 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 7.0 4 11.0 5 44.0 6 112.0 7 244.0 8 554.0 9 1132.0 10 2029.0 11 3515.0 12 5344.0 13 7421.0 14 9329.0 15 13188.0 16 18268.0 17 25294.0 18 20924.0 19 11005.0 20 8703.0 21 8676.0 22 8886.0 23 9400.0 24 10796.0 25 12438.0 26 14803.0 27 17505.0 28 21862.0 29 26334.0 30 32242.0 31 39795.0 32 49550.0 33 64455.0 34 85986.0 35 119276.0 36 166374.0 37 213168.0 38 115100.0 39 4627.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.73019309807842 20.090265142970615 15.810770676544388 19.36877108240657 2 15.835897150092348 22.62440207845497 39.73682308488766 21.80287768656502 3 18.575838452797164 28.449270503615914 31.85558975787948 21.119301285707444 4 12.254560051968092 18.643988098192523 37.82681424629288 31.274637603546505 5 12.046354640166358 39.677401938758095 32.9315443535325 15.344699067543043 6 28.042131771504103 38.750101228059634 17.598792055360647 15.608974945075616 7 25.629357171841722 36.882986152836054 20.743751708908267 16.743904966413968 8 27.442426268964475 33.163357271048255 19.683785311177232 19.710431148810038 9 28.491304393258076 15.736035304894827 20.819560500036573 34.95309980181053 10-11 24.329870245220075 28.352433870865212 26.181015798543534 21.136680085371175 12-13 26.762856379009005 26.59343511091411 27.689905522933046 18.95380298714384 14-15 23.49017396661739 26.120638044502886 28.670551942892097 21.718636045987623 16-17 19.707470500184172 29.886615865419365 28.948003173118703 21.457910461277763 18-19 19.740263359091042 26.155875074234103 34.78971502811753 19.314146538557324 20-21 21.443831943632315 24.93193723204299 35.120693040726195 18.503537783598496 22-23 20.706108656684187 25.356404704546453 35.079070184612476 18.85841645415688 24-25 20.11240949833702 25.581193853484507 35.44473765674655 18.86165899143192 26-27 20.01293980422233 25.775985517172018 35.210809525820615 19.00026515278504 28-29 19.666778706996542 25.69026712040098 35.40041936753524 19.24253480506724 30-31 19.710169915107755 25.83104971538588 35.3799252349144 19.078855134591958 32-33 19.66908627337608 25.909006997586197 35.03447417093059 19.387432558107136 34-35 19.866997214377953 25.829482313172186 34.97048494553713 19.33303552691273 36-37 20.266750611298043 25.62936703658035 34.58370777515378 19.520174576967822 38-39 20.02776317869237 25.896043964752607 34.63682966793479 19.43936318862023 40-41 20.04186736457218 25.98184325252299 34.42715596681785 19.549133416086978 42-43 20.076027715154254 26.30880261791001 33.9027357264082 19.712433940527536 44-45 20.162697979032302 26.657032851773206 33.369587129060754 19.810682040133738 46-47 20.625334434581873 26.93789193338346 32.291544666527635 20.14522896550703 48-49 20.82707460921615 27.34986246045575 31.376388130585504 20.4466747997426 50-51 21.086913266116937 27.796971474176026 30.76171181938391 20.354403440323132 52-53 21.106954581789875 28.050259754056533 30.595901754972598 20.246883909180998 54-55 20.727501029696178 28.046616283393288 30.472214748035743 20.7536679388748 56-57 20.92042612627047 28.45021560582101 29.97735093940048 20.652007328508034 58-59 21.024734520787707 27.81695322602415 30.34761558522982 20.810696667958325 60-61 20.96299469860046 27.8487620779257 30.682764181766252 20.505479041707595 62-63 21.093411552543046 27.791490134287244 30.85145243824759 20.26364587492212 64-65 20.927649584594874 27.898627391050308 30.68094047615938 20.49278254819544 66-67 20.92926052575895 28.183807516041924 30.32161351866994 20.565318439529186 68-69 21.167244428538215 28.10269445148324 30.289743006991483 20.44031811298706 70-71 21.00879667847452 28.312991776298603 30.16384706214276 20.514364483084112 72-73 21.062636441136235 28.315448859228017 29.684105298814252 20.9378094008215 74-75 20.965628824746236 28.68411095644617 29.523525060292478 20.826735158515117 76-77 20.886585190635625 28.6945258382351 29.719565132206256 20.69932383892302 78-79 20.812980590678354 28.658258094038487 29.57327705751915 20.955484257764013 80-81 20.65219284343401 29.04912345476901 29.412986384203027 20.88569731759395 82-83 20.679843886603575 28.8818926572367 29.150920065900614 21.28734339025911 84-85 20.65023779470717 28.689061704711357 29.141705442827604 21.518995057753877 86-87 20.787178256920757 28.595393737161405 29.006011008426558 21.611416997491276 88-89 20.7574263353253 28.50889854031284 29.04474850058235 21.688926623779512 90-91 20.392040244623498 28.729023656865298 29.221588380819192 21.657347717692012 92-93 20.30012269276217 28.667394637917027 29.099692876244017 21.932789793076786 94-95 20.196804763509483 28.484748741071247 29.079708498019414 22.238737997399856 96-97 20.57171219770244 28.458083799746163 28.771739627348662 22.19846437520273 98-99 20.300465436243073 28.431667080291017 28.735702747771867 22.532164735694042 100-101 20.170537667367952 28.676478271773508 28.879200744012373 22.273783316846167 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2159.0 1 1521.5 2 1046.5 3 1217.0 4 1055.5 5 1033.0 6 1758.0 7 3814.5 8 6143.0 9 6962.0 10 5814.0 11 4504.0 12 4237.5 13 4218.5 14 4452.5 15 4632.0 16 4655.0 17 4800.5 18 4967.5 19 5129.0 20 5405.0 21 5790.0 22 6491.0 23 7337.5 24 8157.0 25 9410.5 26 10936.5 27 12441.5 28 13579.0 29 15070.5 30 17453.0 31 19325.0 32 22066.0 33 24702.0 34 26912.5 35 28980.0 36 31741.0 37 34804.5 38 35106.0 39 34131.0 40 33437.5 41 33735.5 42 33923.5 43 34730.0 44 36181.0 45 36647.0 46 35892.5 47 35121.0 48 37749.0 49 37268.5 50 33537.0 51 31400.0 52 29839.0 53 29879.5 54 29281.0 55 27325.0 56 24764.0 57 22477.5 58 21859.0 59 20775.5 60 18112.5 61 15435.5 62 12863.0 63 9673.5 64 6560.0 65 4768.5 66 3397.0 67 2625.0 68 1876.5 69 1087.0 70 780.0 71 587.0 72 482.0 73 446.5 74 352.0 75 252.5 76 175.0 77 88.0 78 46.0 79 30.5 80 21.5 81 13.5 82 10.5 83 5.5 84 1.5 85 0.0 86 0.5 87 1.0 88 0.5 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019505449770419115 2 0.0038314276334751834 3 1.7415580152159923E-4 4 0.0 5 0.0028735707251063875 6 0.0 7 2.6123370228239883E-4 8 0.0 9 8.707790076079961E-5 10-11 0.0 12-13 0.00661792045782077 14-15 0.0038749665838555833 16-17 0.0 18-19 2.6123370228239883E-4 20-21 7.401621564667968E-4 22-23 0.001610941164074793 24-25 5.660063549451976E-4 26-27 2.1769475190199904E-4 28-29 8.707790076079961E-5 30-31 0.0 32-33 8.707790076079961E-5 34-35 0.0 36-37 0.001828635915976792 38-39 0.009883341736350757 40-41 7.401621564667968E-4 42-43 0.0 44-45 0.0010013958587491956 46-47 3.0477265266279867E-4 48-49 0.0 50-51 0.0014367853625531937 52-53 2.6123370228239883E-4 54-55 0.0 56-57 4.353895038039981E-4 58-59 1.7415580152159923E-4 60-61 0.001828635915976792 62-63 4.353895038039981E-5 64-65 0.0 66-67 0.0 68-69 0.0 70-71 7.837011068471966E-4 72-73 6.966232060863969E-4 74-75 0.0031783433777691863 76-77 1.7415580152159923E-4 78-79 4.7892845418439793E-4 80-81 0.0021769475190199904 82-83 8.707790076079961E-5 84-85 0.0028300317747259876 86-87 3.4831160304319846E-4 88-89 0.003004187576247587 90-91 0.0017415580152159923 92-93 0.0 94-95 0.0 96-97 3.918505534235983E-4 98-99 0.0010449348091295953 100-101 0.002525259122063189 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1148397.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.398885778328406 #Duplication Level Percentage of deduplicated Percentage of total 1 74.57586635308317 36.093888374427564 2 13.61804984882378 13.181968783136089 3 4.781546485490459 6.942645665850611 4 2.180030525049247 4.220441935005112 5 1.2211949076882151 2.955223642513911 6 0.7292962075402905 2.117827430838636 7 0.5036190535693224 1.706222073464404 8 0.3557698973208067 1.3775093299037877 9 0.26734241972954187 1.1645167712572826 >10 1.5586710939665271 14.20293870145922 >50 0.13668014089461233 4.576975235784542 >100 0.06723392262102003 5.752489750263072 >500 0.0027110447440904426 0.8988865569910933 >1k 0.0014458905301815692 1.3094912181935254 >5k 1.8073631627269616E-4 0.8629345936859805 >10k+ 3.614726325453923E-4 2.636039937225243 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16897 1.4713552891552313 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13238 1.1527372502714655 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9865 0.8590234910052883 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2875 0.2503489646872989 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2706 0.23563279945872379 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2425 0.2111639093449391 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1937 0.16866989377366887 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1352 0.11772932182860109 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1345 0.11711977652327549 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1215 0.10579964942437155 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 8.707790076079961E-5 0.0 16-17 0.0 0.0 0.0 8.707790076079961E-5 0.0 18-19 0.0 0.0 0.0 8.707790076079961E-5 0.0 20-21 0.0 0.0 0.0 2.6123370228239883E-4 0.0 22-23 0.0 0.0 0.0 7.837011068471966E-4 0.0 24-25 0.0 0.0 0.0 0.0015674022136943932 0.0 26-27 0.0 0.0 0.0 0.0023946422709219895 0.0 28-29 0.0 0.0 0.0 0.004440972938800781 0.0 30-31 0.0 0.0 0.0 0.00931733538140556 0.0 32-33 0.0 0.0 0.0 0.014063080972869138 0.0 34-35 0.0 0.0 0.0 0.01994083927422311 0.0 36-37 0.0 0.0 0.0 0.02747307769003228 0.0 38-39 8.707790076079961E-5 0.0 0.0 0.03914151639197943 0.0 40-41 8.707790076079961E-5 0.0 0.0 0.06038852417761453 0.0 42-43 8.707790076079961E-5 0.0 0.0 0.08268046677237924 0.0 44-45 8.707790076079961E-5 0.0 0.0 0.10884737595099953 0.0 46-47 8.707790076079961E-5 0.0 0.0 0.1599185647472085 0.0 48-49 2.6123370228239883E-4 0.0 0.0 0.19579465986065794 0.0 50-51 2.6123370228239883E-4 0.0 0.0 0.24098809035551294 0.0 52-53 2.6123370228239883E-4 0.0 0.0 0.29484577197606754 0.0 54-55 2.6123370228239883E-4 0.0 0.0 0.35906572378715723 0.0 56-57 3.4831160304319846E-4 0.0 0.0 0.4776658246233663 0.0 58-59 3.4831160304319846E-4 0.0 0.0 0.5834654740477379 0.0 60-61 3.4831160304319846E-4 0.0 0.0 0.6809056449990727 0.0 62-63 3.4831160304319846E-4 0.0 0.0 0.7861828270188793 0.0 64-65 3.4831160304319846E-4 0.0 0.0 0.8883687435616777 0.0 66-67 3.4831160304319846E-4 0.0 0.0 1.036488252755798 0.0 68-69 3.4831160304319846E-4 0.0 0.0 1.2189599938000535 0.0 70-71 3.4831160304319846E-4 0.0 0.0 1.3483142153802212 0.0 72-73 3.4831160304319846E-4 0.0 0.0 1.4988283668452635 0.0 74-75 4.789284541843979E-4 0.0 0.0 1.7057689980033037 0.0 76-77 6.095453053255973E-4 0.0 0.0 1.9363077402675208 0.0 78-79 6.095453053255973E-4 0.0 0.0 2.1817368035618343 0.0 80-81 6.095453053255973E-4 0.0 0.0 2.430300671283537 0.0 82-83 6.095453053255973E-4 0.0 0.0 2.7256253717137886 0.0 84-85 6.095453053255973E-4 0.0 0.0 3.0897416137450726 0.0 86-87 6.095453053255973E-4 0.0 0.0 3.4066616335640028 0.0 88-89 6.095453053255973E-4 0.0 0.0 3.8014292966630876 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7145 0.0 46.543423 1 GTATCAA 12585 0.0 40.995773 1 TATCAAC 16735 0.0 30.738989 2 ATCAACG 16720 0.0 30.709751 3 TCAACGC 16870 0.0 30.436693 4 CAACGCA 17120 0.0 29.992231 5 AACGCAG 17465 0.0 29.376411 6 CATATAG 325 0.0 29.230312 3 TGAACCG 125 7.3917854E-6 26.599586 5 ACGCAGA 19560 0.0 26.132864 7 CGCAGAG 19585 0.0 26.052128 8 GCAGAGT 20055 0.0 25.109941 9 AGTACTT 12445 0.0 23.79941 12-13 GAGTACT 12200 0.0 23.634888 12-13 GTACATG 9690 0.0 22.650652 1 GTACTTT 13080 0.0 21.87896 14-15 TACATGG 9970 0.0 21.58191 2 AGAGTAC 19260 0.0 21.07746 10-11 TACTTTT 13965 0.0 21.07064 14-15 ACGCAGT 160 4.98319E-5 20.784546 8 >>END_MODULE