FastQCFastQC Report
Thu 26 May 2016
SRR936963_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936963_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15719
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1120.7125135186716712No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT930.5916406896112985No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT540.34353330364527007No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT420.26719256950187675No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT370.2353839302754628No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200.12723455690565558No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.12723455690565558No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAAGT250.004920290357.0268364
GGTATCA503.9635597E-757.0268361
GTATCAA1050.054.311271
TATCAAC1500.038.017892
ATCAACG1550.036.7915083
TCAACGC1701.8189894E-1233.54524
AACGCAG1753.6379788E-1232.5867656
CAACGCA1753.6379788E-1232.5867655
TACTTTT950.032.51530514-15
AGTACTT1000.030.88953812-13
ACGCAGA1951.2732926E-1129.2445327
AGAGTAC1800.029.04144510-11
CGCAGAG1954.3655746E-1026.8074877
CAGAGTA2000.026.13739
GCAGAGT2005.875336E-1026.13738
GAGTACT951.0941221E-825.01177212-13
GTACTTT1001.8875653E-823.76118314-15
ACTTTTT1258.587449E-920.90984216-17
CTTTTTT1808.185452E-1118.48091916-17
GAGTACA1053.8363656E-418.1037586
Produced by FastQC (version 0.11.5)