FastQCFastQC Report
Thu 26 May 2016
SRR936963_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936963_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15719
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1350.8588332591131752No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT990.6298110566829951No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT770.489853044086774No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT610.38806539856224953No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA480.3053629365735734No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT450.286277753037725No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT280.1781283796679178No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT260.16540492397735226RNA PCR Primer, Index 33 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT240.1526814682867867TruSeq Adapter, Index 11 (95% over 21bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220.13995801259622112No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG200.12723455690565558RNA PCR Primer, Index 33 (96% over 29bp)
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG180.11451110121509002RNA PCR Primer, Index 39 (96% over 30bp)

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGGGA156.495954E-495.08
GGTATCA750.063.3333361
GTATCAA1051.8189894E-1145.2380942
TATCAAC1508.094503E-1031.6666683
TCAACGC1551.1514203E-930.645165
ATCAACG1551.1514203E-930.645164
CAACGCA1652.246452E-928.7878786
AACGCAG1703.092282E-927.9411757
GTACTTT853.3614924E-927.94117514-15
GAGTACT953.601599E-1027.512-13
ACTTTTT1101.9717845E-923.75000216-17
CGCAGAG1801.7094135E-723.758
ACGCAGA1852.2275162E-723.1081097
CAGAGTA1650.023.03030410-11
AGTACTT957.840059E-620.012-13
TACTTTT957.840059E-620.014-15
AGAGTAC1603.4924597E-1019.29687510-11
GCAGAGT1759.16691E-519.09
TCTCGTA1050.00708646713.57142834-35
CTTTTTT1756.7369983E-613.57142816-17
Produced by FastQC (version 0.11.5)