FastQCFastQC Report
Thu 26 May 2016
SRR936962_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936962_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14468
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1200.8294166436273155No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT840.5805916505391209No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT600.41470832181365774No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT460.31794304672380425No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240.16588332872546308No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.13132430190765826No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170.11750069118053635No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA900.058.0555571
TCAACGC1150.045.434784
TATCAAC1150.045.434782
ATCAACG1150.045.434783
CAACGCA1201.8189894E-1243.5416685
AACGCAG1303.6379788E-1240.192316
GGTATCA500.00156357338.01
ACGCAGA1409.094947E-1237.3214267
CGCAGAG1409.094947E-1237.3214268
GCAGAGT1403.8016879E-1033.928579
AGTACTT905.456968E-1231.66666812-13
GTACTTT853.3360266E-927.94117514-15
GAGTACT853.3360266E-927.94117512-13
TACTTTT1053.8198777E-1127.14285714-15
AGAGTAC1300.025.57692310-11
CAGAGTA1351.8189894E-1224.62963110-11
ACTTTTT1001.8841092E-823.7516-17
CTTTTTT1452.1609594E-919.65517216-17
Produced by FastQC (version 0.11.5)