FastQCFastQC Report
Thu 26 May 2016
SRR936962_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936962_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14468
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1030.7119159524467791No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT990.6842687309925353No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT570.39397290572297483No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT500.3455902681780481No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA430.29720763063312133No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT370.2557367984517556No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260.17970693945258503No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT240.16588332872546308No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG240.16588332872546308RNA PCR Primer, Index 39 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT230.15897152336190212No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT190.13132430190765826RNA PCR Primer, Index 33 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT180.12441249654409732No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT170.11750069118053635No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT160.1105888858169754TruSeq Adapter, Index 11 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT150.10367708045341444No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT150.10367708045341444No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG150.10367708045341444RNA PCR Primer, Index 33 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT150.10367708045341444RNA PCR Primer, Index 11 (95% over 23bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA655.9480953E-1058.461541
TGGTATC250.004921782857.02
AGTACAT554.7232817E-543.181827
GTATCAA1159.213727E-833.0434761
TATCAAC1201.3390854E-731.6666682
ATCAACG1201.3390854E-731.6666683
TCAACGC1251.9159597E-730.44
TACATGG804.2801807E-429.6875029
GAGTACA804.2801807E-429.6875026
AACGCAG1353.7604514E-728.1481516
CAACGCA1353.7604514E-728.1481515
GTACATG908.525568E-426.3888918
GTACTTT1153.270543E-922.7173914-15
GAGTACT1153.270543E-922.7173912-13
ACGCAGA1702.8081886E-622.352947
CGCAGAG1702.8081886E-622.352948
CAGAGTA1701.8189894E-1220.95588110-11
AGAGTAC1759.101693E-519.05
ACTTTTT1403.1521267E-818.66071316-17
GCAGAGT1901.7101332E-417.53
Produced by FastQC (version 0.11.5)