FastQCFastQC Report
Thu 26 May 2016
SRR936961_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936961_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14817
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1030.695147465748802No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT930.6276574205304718No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT620.41843828035364783No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT510.3441992306134845No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330.22271714922048996No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.1349800904366606No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA500.001564810938.01
GTATCAA804.2852075E-429.6874981
TATCAAC1056.464008E-527.1428551
TCAACGC1108.872164E-525.9090923
ATCAACG1108.872164E-525.9090922
AGTACTT751.0029726E-625.33333412-13
AACGCAG1201.602356E-423.755
CAACGCA1201.602356E-423.754
CAGAGTA1301.1544737E-421.9230779
CGCAGAG1353.5574954E-421.111117
AGAGTAC1301.3487806E-820.09615510-11
ACGCAGA1455.7623297E-419.6551726
TACTTTT857.625253E-519.55882314-15
GCAGAGT1507.2405E-419.08
CTTTTTT1050.00707376113.57142716-17
Produced by FastQC (version 0.11.5)