FastQCFastQC Report
Thu 26 May 2016
SRR936961_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936961_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14817
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1120.7558885064452993No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT940.6344064250523047No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT520.35094823513531753No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT510.3441992306134845No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA440.2969561989606533No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400.2699601808733212No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT390.26321117635148816No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT310.20921914017682391RNA PCR Primer, Index 33 (95% over 22bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250.16872511304582574No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT210.14172909495849362No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG170.1147330768711615RNA PCR Primer, Index 33 (96% over 29bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG160.10798407234932847No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT160.10798407234932847TruSeq Adapter, Index 11 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT150.10123506782749545No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA451.867611E-763.5457651
GTATCAA652.420249E-643.8445932
TCAACGC1201.6027424E-423.7491575
TATCAAC1201.6027424E-423.7491573
ATCAACG1201.6027424E-423.7491574
AACGCAG1302.756827E-421.9222977
CAACGCA1353.5583507E-421.1103616
ACGCAGA1455.7637104E-419.6544748
CGCAGAG1455.7637104E-419.6544749
AGTACTT951.7899238E-417.49937612-13
GTACTTT950.003640994414.99946614-15
CAGAGTA1602.6489815E-614.8432239
GAGTACT1000.00512023714.24949312-13
TACTTTT1000.00512023714.24949314-15
ACTTTTT1050.007075425713.57094616-17
AGAGTAC1452.9183357E-413.10298259
Produced by FastQC (version 0.11.5)