FastQCFastQC Report
Thu 26 May 2016
SRR936960_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936960_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15343
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1180.7690803623802385No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT910.5931043472593365No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT450.29329335853483673No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT430.28025809815551067No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT380.24766994720719546No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA310.20204653587955418No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAAGA200.00203700771.232844
GGTATCA851.8189894E-1255.8688961
AGTCTGA205.312185E-447.4885642-43
GGTGTAA205.312185E-447.4885626-27
GTATCAA1207.2759576E-1139.5738032
TATCAAC1403.8562575E-1033.9204033
GTACTTT859.822543E-1130.72789414-15
AACGCAG1601.6134436E-929.6803517
TCAACGC1601.6134436E-929.6803515
ATCAACG1601.6134436E-929.6803514
CAACGCA1601.6134436E-929.6803516
GAGTACT901.9099389E-1029.02078812-13
CGCAGAG1652.242814E-928.7809499
ACGCAGA1703.086825E-927.9344488
CAGAGTA1600.025.22829810-11
ACTTTTT1101.9717845E-923.7442816-17
GAGTACA601.6611256E-423.7442812-13
GCAGAGT1601.6717586E-623.744289
ACATGGG652.856622E-421.91779916-17
GTACATG600.004666758719.78690114-15
Produced by FastQC (version 0.11.5)