FastQCFastQC Report
Thu 26 May 2016
SRR936960_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936960_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15343
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1140.7430098416215865No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT960.6256924982076517No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT590.38454018119011923No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT500.3258815094831519No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA490.31936387929348886No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT350.22811705663820636No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT240.15642312455191293No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT240.15642312455191293No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT220.14338786417258684TruSeq Adapter, Index 11 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT190.12383497360359773RNA PCR Primer, Index 33 (95% over 22bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.11079971322427165No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT170.11079971322427165No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT160.10428208303460862RNA PCR Primer, Index 23 (95% over 23bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.10428208303460862No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1251.8189894E-1241.81
ATCAACG1604.3655746E-1132.656253
TGGTATC600.003831378931.6666682
TCAACGC1656.184564E-1131.6666664
TATCAAC1708.913048E-1130.7352962
CAACGCA1708.913048E-1130.7352965
AACGCAG1601.6098056E-929.6875026
GTGGTAT650.00566798229.230771
CGCAGAG1703.07773E-927.9411778
ACGCAGA1805.666152E-926.3888877
AGTACTT701.7072243E-523.74999812-13
TACTTTT804.7888756E-520.7812514-15
AGAGTAC1401.4060788E-920.35714110-11
CAGAGTA1602.6551843E-617.81259
GCAGAGT1700.001681229916.7647088
CTTTTTT1154.056655E-516.5217416-17
ACTTTTT1100.00963064612.95454616-17
Produced by FastQC (version 0.11.5)