##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936959_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 190690 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.895500550631915 33.0 31.0 34.0 30.0 34.0 2 31.869300959672767 33.0 31.0 34.0 30.0 34.0 3 31.791252818710998 33.0 31.0 34.0 30.0 34.0 4 35.44102994388798 37.0 35.0 37.0 33.0 37.0 5 35.4958361738948 37.0 35.0 37.0 33.0 37.0 6 35.643971891551736 37.0 35.0 37.0 33.0 37.0 7 35.633305364728095 37.0 35.0 37.0 33.0 37.0 8 35.6086580313598 37.0 35.0 37.0 33.0 37.0 9 37.14240390162043 39.0 37.0 39.0 34.0 39.0 10-11 37.057173947244216 39.0 37.0 39.0 33.0 39.0 12-13 36.934634747495934 39.0 37.0 39.0 33.0 39.0 14-15 37.74553988148304 40.0 37.0 41.0 32.5 41.0 16-17 37.75824899050815 40.0 37.0 41.0 32.5 41.0 18-19 37.93537678955373 40.0 37.5 41.0 33.0 41.0 20-21 38.01119356022865 40.0 37.5 41.0 33.0 41.0 22-23 37.87559913996539 40.0 37.0 41.0 33.0 41.0 24-25 37.66168912895275 40.0 36.5 41.0 33.0 41.0 26-27 37.46222402852798 39.5 36.0 41.0 33.0 41.0 28-29 37.373202580103836 39.5 36.0 41.0 33.0 41.0 30-31 37.003036341706434 39.0 35.0 41.0 33.0 41.0 32-33 36.520116419319315 38.0 35.0 40.5 32.0 41.0 34-35 36.187193874875454 38.0 35.0 40.0 31.0 41.0 36-37 35.84629241176779 38.0 35.0 40.0 31.0 41.0 38-39 35.47978656458126 37.5 35.0 40.0 30.0 41.0 40-41 35.07382400755152 37.0 35.0 40.0 29.5 41.0 42-43 34.69750904609576 37.0 35.0 40.0 27.5 41.0 44-45 34.13721222927264 37.0 34.5 40.0 23.5 41.0 46-47 33.36205359483979 36.0 33.5 40.0 17.5 41.0 48-49 32.70937123079344 36.0 33.0 40.0 8.0 41.0 50-51 31.90627720383869 35.0 32.0 39.0 2.0 40.5 52-53 31.980415858199173 35.0 33.0 39.0 2.0 40.5 54-55 32.1713278095338 35.0 33.0 40.0 2.0 41.0 56-57 32.035638995227856 35.0 33.0 40.0 2.0 41.0 58-59 31.822245529393257 35.0 33.0 40.0 2.0 41.0 60-61 31.465082070376003 35.0 32.5 39.5 2.0 41.0 62-63 31.086984110336147 35.0 32.0 39.0 2.0 41.0 64-65 30.648568357019244 35.0 31.0 39.0 2.0 41.0 66-67 30.22769154124495 35.0 31.0 38.0 2.0 40.0 68-69 29.794803083538728 35.0 30.0 37.0 2.0 40.0 70-71 29.36479888824794 35.0 29.5 37.0 2.0 39.0 72-73 28.919738843148565 35.0 29.0 36.0 2.0 39.0 74-75 28.490704808852065 35.0 28.5 36.0 2.0 39.0 76-77 27.987146677854106 34.5 27.5 35.0 2.0 37.5 78-79 27.527125176988832 34.0 26.0 35.0 2.0 37.0 80-81 27.128176621742092 34.0 25.5 35.0 2.0 36.5 82-83 26.68422046253081 34.0 24.5 35.0 2.0 36.0 84-85 26.292052546017096 34.0 23.5 35.0 2.0 36.0 86-87 25.893754260842204 34.0 20.0 35.0 2.0 35.0 88-89 25.360852692852276 33.0 15.0 35.0 2.0 35.0 90-91 24.868338664848707 33.0 6.0 35.0 2.0 35.0 92-93 24.504766899155698 33.0 2.0 35.0 2.0 35.0 94-95 24.066608107399443 33.0 2.0 35.0 2.0 35.0 96-97 23.516909643924695 32.5 2.0 35.0 2.0 35.0 98-99 22.90753316901778 32.0 2.0 35.0 2.0 35.0 100-101 21.215464890660236 29.5 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5224016167015186 1101 2 0.23724251609481328 1101 3 0.2574063279188685 1101 4 0.32790416753549323 1101 5 0.29856764057549867 1101 6 0.5045244347689604 1101 7 0.5012396617304802 1101 8 -0.0354554617136813 1101 9 0.45549269411955606 1101 10-11 0.4312861600259197 1101 12-13 0.6158153460202911 1101 14-15 0.8694148642135247 1101 16-17 0.873449259888389 1101 18-19 0.7853343928611523 1101 20-21 0.6486740611157558 1101 22-23 0.818189437838619 1101 24-25 1.023516112107437 1101 26-27 1.011229897426233 1101 28-29 0.7121649451553509 1101 30-31 0.8701579945871032 1101 32-33 0.7904401553410239 1101 34-35 0.5981537006288562 1101 36-37 0.3053547385904949 1101 38-39 0.34688860023967294 1101 40-41 0.47170028342853954 1101 42-43 0.20322852465008623 1101 44-45 -0.012244826450185542 1101 46-47 -0.21982106714430927 1101 48-49 0.1429485622871951 1101 50-51 0.21050145663811648 1101 52-53 0.29789956219698865 1101 54-55 -0.28156749680828597 1101 56-57 -0.03186990918127108 1101 58-59 -0.0638033221790586 1101 60-61 -0.16397816447151214 1101 62-63 -0.14544555237425527 1101 64-65 0.15614449017927257 1101 66-67 0.313607607237401 1101 68-69 0.17279201404608813 1101 70-71 0.12334569676103513 1101 72-73 -0.10486276221492119 1101 74-75 -0.10358013137287259 1101 76-77 -0.39072945434732276 1101 78-79 -0.32756973557392755 1101 80-81 -0.14140348637048916 1101 82-83 -0.07981609207329399 1101 84-85 -0.002331237109498119 1101 86-87 -0.20019257095729515 1101 88-89 0.008647293978068404 1101 90-91 0.034762588286131546 1101 92-93 -0.03683373093014097 1101 94-95 0.2705919064381064 1101 96-97 0.8932218600734139 1101 98-99 1.125772584605155 1101 100-101 1.0559909229193032 1102 1 0.21649847685677415 1102 2 0.23292667783737286 1102 3 0.1437752307111282 1102 4 -0.037146232186444195 1102 5 0.11597002732094808 1102 6 -0.07550684781706707 1102 7 -0.0048430633303482296 1102 8 -0.12388669795513607 1102 9 -0.4033301537543892 1102 10-11 0.04219451363500326 1102 12-13 0.037724742382245324 1102 14-15 0.005450665395308363 1102 16-17 -0.023043035326530514 1102 18-19 0.05526255877471442 1102 20-21 -0.09811078420923991 1102 22-23 -0.34514621272331425 1102 24-25 0.05749247637577071 1102 26-27 -0.21971089738318028 1102 28-29 -0.1076705316144313 1102 30-31 7.917566077253468E-4 1102 32-33 -0.05652137866429996 1102 34-35 -0.047585495292821633 1102 36-37 0.027852131708314687 1102 38-39 -0.4722372252730125 1102 40-41 -0.39888249956300115 1102 42-43 0.07705542767406826 1102 44-45 0.24496754755050887 1102 46-47 0.08627034752375096 1102 48-49 0.04966562867578972 1102 50-51 0.35729788810608554 1102 52-53 0.4997012074292897 1102 54-55 0.7124946049180529 1102 56-57 0.7459513166326524 1102 58-59 0.5982578526558378 1102 60-61 0.8653492599289017 1102 62-63 0.5705201526425441 1102 64-65 0.8947755873870165 1102 66-67 0.8388943642759834 1102 68-69 0.6797002517937436 1102 70-71 0.7809896545874757 1102 72-73 0.9242329393260391 1102 74-75 1.4785076915908633 1102 76-77 1.3111880523147121 1102 78-79 1.2224679193907342 1102 80-81 1.4504311793692857 1102 82-83 1.7172381310758205 1102 84-85 1.8254697129542272 1102 86-87 2.080105192917113 1102 88-89 2.175342926002166 1102 90-91 2.2497724365076586 1102 92-93 2.2457522961814362 1102 94-95 1.8589796946434127 1102 96-97 1.9919213155246958 1102 98-99 2.093273453565878 1102 100-101 2.271753870434079 1103 1 0.23401106668306326 1103 2 0.35896639944660436 1103 3 0.3453539100524914 1103 4 0.36417216310581324 1103 5 0.37147277162016223 1103 6 0.38796851007349886 1103 7 0.396588498939785 1103 8 0.27120751355394646 1103 9 0.3899703611553633 1103 10-11 0.33761690937992483 1103 12-13 0.27519149958249756 1103 14-15 0.14419338027111195 1103 16-17 0.72583944076694 1103 18-19 0.5576673570545765 1103 20-21 0.5296264420681354 1103 22-23 0.5215855249556327 1103 24-25 0.7419546503134171 1103 26-27 0.88461491034613 1103 28-29 0.6179419846533136 1103 30-31 0.6405529742844038 1103 32-33 0.7394527060977722 1103 34-35 0.8506915374690251 1103 36-37 0.8791365769072144 1103 38-39 1.1438155105386798 1103 40-41 1.1451960383196464 1103 42-43 1.0407146819036868 1103 44-45 1.1596820838488142 1103 46-47 0.9505831410506005 1103 48-49 1.4661766907478793 1103 50-51 1.6187701904882523 1103 52-53 1.8182945418942893 1103 54-55 1.7057894614789042 1103 56-57 1.4467661188733594 1103 58-59 1.5173151717301359 1103 60-61 1.5354958111652515 1103 62-63 1.8605529710661273 1103 64-65 1.9645147375030092 1103 66-67 1.761558880770071 1103 68-69 1.899951482484628 1103 70-71 1.9253482807403621 1103 72-73 1.7423594600073002 1103 74-75 1.665615143636355 1103 76-77 1.7835045803518312 1103 78-79 1.7209260193171758 1103 80-81 1.5842794224519565 1103 82-83 1.451606942256717 1103 84-85 2.0263693904283393 1103 86-87 2.1711621724904724 1103 88-89 2.1696845771083737 1103 90-91 1.8641276676512142 1103 92-93 2.246388860431985 1103 94-95 2.0363264948471134 1103 96-97 2.2169114134141275 1103 98-99 2.3295378116261958 1103 100-101 3.0219377667731244 1104 1 0.2632746325745323 1104 2 0.46720283355513104 1104 3 -0.06084764033510126 1104 4 0.33742797705929917 1104 5 0.3626549421627985 1104 6 0.3342863395308626 1104 7 0.5804063283971459 1104 8 0.5385524747942512 1104 9 0.6338657099925697 1104 10-11 0.49309171558147824 1104 12-13 0.6544066158615607 1104 14-15 0.6848910546897216 1104 16-17 0.7699770376661661 1104 18-19 0.6102351865119431 1104 20-21 0.4129597754014682 1104 22-23 0.5371378505370288 1104 24-25 0.45595658829791574 1104 26-27 0.3334521196484559 1104 28-29 0.7126609769013754 1104 30-31 0.19714212157123256 1104 32-33 0.20422983788071036 1104 34-35 0.27603068475584536 1104 36-37 0.4988071195428745 1104 38-39 0.5189124097634803 1104 40-41 0.6528510770793403 1104 42-43 0.25263328655485395 1104 44-45 0.5826956497402946 1104 46-47 1.2679765519033097 1104 48-49 1.5188414194300535 1104 50-51 1.057723678860338 1104 52-53 0.7123836891811095 1104 54-55 0.9226983762075882 1104 56-57 1.3911459638345995 1104 58-59 1.4701252492495094 1104 60-61 1.3634027879094397 1104 62-63 1.3937409555622509 1104 64-65 1.524398458433243 1104 66-67 1.8859290358088323 1104 68-69 1.7448158235698976 1104 70-71 1.8427901412054801 1104 72-73 1.784026126673968 1104 74-75 1.4926500273572856 1104 76-77 1.8367507043828368 1104 78-79 2.2202477247435333 1104 80-81 2.404628259661255 1104 82-83 2.298457717450514 1104 84-85 2.460962413684154 1104 86-87 2.484728063963342 1104 88-89 2.5665838019145717 1104 90-91 2.3266276676512128 1104 92-93 1.5125710309746196 1104 94-95 1.568012541358744 1104 96-97 1.5285393203908733 1104 98-99 1.8960106798432506 1104 100-101 2.543788541966922 1105 1 0.2121252072871762 1105 2 0.19838099447467172 1105 3 0.18857764702121926 1105 4 0.610129126484587 1105 5 0.024005516875440946 1105 6 0.028539213094084914 1105 7 0.27853851230519666 1105 8 0.2961674173229909 1105 9 0.33487145711900723 1105 10-11 0.33454286500676034 1105 12-13 0.2139612135627118 1105 14-15 0.3544312845747726 1105 16-17 0.6447615204247867 1105 18-19 1.012174841684356 1105 20-21 0.9326436834474521 1105 22-23 0.4600545172036945 1105 24-25 0.7619192319760728 1105 26-27 0.9504061426369645 1105 28-29 0.5894569539128725 1105 30-31 0.5661076388126105 1105 32-33 0.7517154700646174 1105 34-35 0.5204990755604442 1105 36-37 0.47007148735896465 1105 38-39 0.9193434442462447 1105 40-41 0.7675062494931311 1105 42-43 0.6490413325318585 1105 44-45 0.22687668422305052 1105 46-47 0.6319851725929624 1105 48-49 0.8937695803495913 1105 50-51 0.9061432190902288 1105 52-53 1.3990216202155992 1105 54-55 1.0425978014949457 1105 56-57 1.4862609063633307 1105 58-59 1.8775965136173305 1105 60-61 1.483805086760011 1105 62-63 1.3991288865967348 1105 64-65 1.7659214469389894 1105 66-67 1.9598514496019348 1105 68-69 2.188493984489437 1105 70-71 2.8185085320100747 1105 72-73 2.491641069202707 1105 74-75 2.505581061840047 1105 76-77 2.002196106681687 1105 78-79 2.584615540835486 1105 80-81 2.889111018281948 1105 82-83 2.5113169128528128 1105 84-85 2.6872555171324315 1105 86-87 2.720647604193225 1105 88-89 3.1092562157076777 1105 90-91 2.8260529550075333 1105 92-93 2.8184618355723217 1105 94-95 2.6619780586001234 1105 96-97 3.121499090505818 1105 98-99 3.0917003350156627 1105 100-101 3.151331645415201 1106 1 0.2458683209374506 1106 2 0.025610131385505497 1106 3 0.4484225766274257 1106 4 -0.06128997165864547 1106 5 -0.09313894343878104 1106 6 -0.2480114908438935 1106 7 -0.13288262623795788 1106 8 -0.16748795913079562 1106 9 0.0709712326750136 1106 10-11 0.11652613372743303 1106 12-13 0.08782624110811099 1106 14-15 0.564082376188729 1106 16-17 0.465576150092204 1106 18-19 0.7535413995296949 1106 20-21 0.37578028822198206 1106 22-23 0.32506931010310325 1106 24-25 0.4987203950040282 1106 26-27 0.3182647429226222 1106 28-29 0.3913468743372732 1106 30-31 0.47939064228128814 1106 32-33 1.0323979838373205 1106 34-35 1.0248349253870046 1106 36-37 1.0174215179649622 1106 38-39 0.8243118180475122 1106 40-41 0.9265936806296295 1106 42-43 1.4232200715647139 1106 44-45 0.8574367740006466 1106 46-47 1.1647590962820118 1106 48-49 1.493927711343268 1106 50-51 1.5086605624895277 1106 52-53 1.5417475945065604 1106 54-55 1.4903635635843138 1106 56-57 1.7363629263592522 1106 58-59 1.5155147956005983 1106 60-61 1.7467361212427726 1106 62-63 2.3432601863314773 1106 64-65 1.9318442178020803 1106 66-67 1.8264985624360506 1106 68-69 1.523489393589621 1106 70-71 1.6346047368662298 1106 72-73 1.4203673495536542 1106 74-75 1.5684636368247418 1106 76-77 1.919381374994277 1106 78-79 1.9268798746449143 1106 80-81 1.924894531850608 1106 82-83 1.9691061395412426 1106 84-85 1.4620718811397744 1106 86-87 1.9588897996635382 1106 88-89 1.8893648670033336 1106 90-91 1.7438120857971704 1106 92-93 2.0485424313691 1106 94-95 2.1148320679859616 1106 96-97 2.5046734426788397 1106 98-99 2.4414248810266805 1106 100-101 1.7223758200734345 1107 1 0.4139156582155614 1107 2 0.7072669361192361 1107 3 0.3032549237674651 1107 4 0.5284536180849386 1107 5 0.4027190447269007 1107 6 0.4336453138898406 1107 7 0.6781627386535618 1107 8 0.4869499106916919 1107 9 0.6098272484541098 1107 10-11 0.431713510453271 1107 12-13 0.5061694363743854 1107 14-15 0.2630961828948486 1107 16-17 0.7480219094610376 1107 18-19 0.5302672377939928 1107 20-21 0.6039854164271077 1107 22-23 0.324477594126769 1107 24-25 0.5304758190671421 1107 26-27 0.40011878631511877 1107 28-29 0.5605776435680454 1107 30-31 1.0484241728532808 1107 32-33 1.0377234276243001 1107 34-35 1.1380499155250732 1107 36-37 0.8497686580044075 1107 38-39 1.072438050789124 1107 40-41 1.297402359130622 1107 42-43 1.2023127737343344 1107 44-45 1.6126635984582336 1107 46-47 1.8049957826725418 1107 48-49 1.9917580860967163 1107 50-51 2.2019544480911044 1107 52-53 2.136422150719582 1107 54-55 2.3209355556947635 1107 56-57 2.2892228869115208 1107 58-59 2.1024970441213107 1107 60-61 2.4595566340632864 1107 62-63 2.1304396735109705 1107 64-65 1.946834355869143 1107 66-67 2.1408969845267833 1107 68-69 1.868854285108359 1107 70-71 2.04407219248753 1107 72-73 1.905179972827817 1107 74-75 2.102585924793182 1107 76-77 1.8872314736136069 1107 78-79 2.2641579811537866 1107 80-81 2.2296282596612578 1107 82-83 2.1367589995017973 1107 84-85 2.356154721376459 1107 86-87 2.010369089604364 1107 88-89 2.2345325198632935 1107 90-91 2.3872045907281354 1107 92-93 2.8814812873848794 1107 94-95 2.9753843362305368 1107 96-97 2.9695649614165127 1107 98-99 2.8633183721509425 1107 100-101 2.6211923881207717 1108 1 0.1216079659078666 1108 2 -0.07871260158444215 1108 3 0.05803577510003777 1108 4 0.1107448900313841 1108 5 0.12314344790992493 1108 6 0.25206138058176464 1108 7 0.29516412806874115 1108 8 0.293088599588998 1108 9 0.4450931319958542 1108 10-11 0.25079902264223364 1108 12-13 0.0375449574050748 1108 14-15 -0.051972656311924936 1108 16-17 0.36489698840508566 1108 18-19 0.3277536594183488 1108 20-21 0.17494663911246988 1108 22-23 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-0.9775171410337897 2316 56-57 -0.2464977143263205 2316 58-59 -0.17340923350910842 2316 60-61 -0.09521790174573042 2316 62-63 -0.40187686052970406 2316 64-65 -0.04657855306101055 2316 66-67 0.3485008748892895 2316 68-69 0.9349020304664499 2316 70-71 -0.10606043350716376 2316 72-73 -0.8409738733260284 2316 74-75 -0.9074936508036338 2316 76-77 -0.7549878013642868 2316 78-79 -0.8284591718081913 2316 80-81 -1.4197970276950649 2316 82-83 -1.038970443469026 2316 84-85 -0.7567100001089493 2316 86-87 -0.708950096956201 2316 88-89 -1.5265196463612867 2316 90-91 -2.020929803613157 2316 92-93 -2.151365750634575 2316 94-95 -2.1792575735837865 2316 96-97 -2.521460679609124 2316 98-99 -3.2243916190073243 2316 100-101 -2.684156860331928 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 546.0 3 116.0 4 121.0 5 135.0 6 157.0 7 186.0 8 246.0 9 300.0 10 415.0 11 686.0 12 1035.0 13 1486.0 14 1762.0 15 2530.0 16 3299.0 17 4660.0 18 3714.0 19 2083.0 20 1944.0 21 2011.0 22 2210.0 23 2324.0 24 2425.0 25 2618.0 26 2916.0 27 3227.0 28 3597.0 29 4226.0 30 5055.0 31 5972.0 32 7475.0 33 9545.0 34 13551.0 35 23205.0 36 25155.0 37 25631.0 38 20456.0 39 3668.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.667817672309745 19.161620084173848 15.815132400633928 15.355429842882481 2 14.712706428278969 24.50735425349865 40.25102098665631 20.528918331566075 3 19.669885588327908 33.6926629579091 31.905636611735066 14.731814842027921 4 10.573110594117122 19.158478497193002 38.70137645273269 31.567034455957188 5 10.17020338306786 41.533405432648394 31.675049087033674 16.62134209725008 6 22.525014961729468 39.593985909725646 19.185661938410174 18.69533719013471 7 21.662492320778792 41.93983691171915 20.24741531853672 16.15025544896534 8 24.978084794784444 30.4928427826799 20.736245912223698 23.792826510311958 9 28.895306101018587 18.460044103748817 21.342539115824845 31.30211067940775 10-11 22.713312192868244 29.112152967365283 24.9485488680751 23.225985971691376 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30.65232106033655 36.806659635535546 15.76704918118805 38-39 17.640561748524924 29.88742467212534 36.92239884355151 15.54961473579822 40-41 15.514259999317767 31.204851207422706 37.92642895190514 15.354459841354393 42-43 14.87078974504294 32.93451195545795 35.62524434368957 16.56945395580954 44-45 15.253823753180995 33.43967258703466 34.28706351496707 17.019440144817274 46-47 15.669615807323975 32.77979021144105 32.56181785274931 18.988776128485657 48-49 16.323837656188108 32.72375265942795 32.467188364887946 18.485221319496002 50-51 17.773464650571125 33.42976669770369 31.992708483600644 16.804060168124533 52-53 16.23436581885335 33.91699194157643 32.36773902459498 17.48090321497524 54-55 15.194768059559754 33.27833115160927 33.11175061713497 18.415150171696 56-57 16.60689785435648 34.836604222030424 30.233400054041148 18.323097869571946 58-59 16.16308175077532 33.141362355497016 31.412049305493607 19.283506588234058 60-61 16.618084980781592 32.30738957614556 32.30555300476197 18.76897243831088 62-63 19.044283173023942 32.20198458301193 31.67514128740771 17.07859095655642 64-65 18.354349029308306 32.61188333337705 32.72703902964931 16.30672860766533 66-67 16.814739791875482 32.93441926122618 32.82264285601835 17.42819809087999 68-69 16.481884723351786 34.342944093186766 32.1174279193011 17.05774326416035 70-71 16.967063057014116 33.90343952118424 30.59348395117422 18.53601347062742 72-73 16.36537679810126 33.35702390474817 30.35496518535031 19.922634111800264 74-75 16.368839194147842 34.015805445613374 31.233459493627723 18.38189586661106 76-77 17.21921710798999 32.4109002576062 31.826695557735796 18.543187076668012 78-79 15.81241209933038 32.25744662986209 33.072430151766405 18.85771111904112 80-81 15.688769152879392 34.3446770669108 31.48666479542718 18.479888984782626 82-83 15.527265857856513 33.408547080307294 30.975294907854412 20.08889215398178 84-85 15.923110629488427 31.73995523707818 30.806388555805402 21.530545577627986 86-87 16.593111916918993 32.10572992375145 31.28027245868777 20.020885700641788 88-89 17.531934103023733 30.38247436479546 31.463522816447476 20.622068715733334 90-91 15.605484944216458 30.761762822867578 32.7721743511162 20.860577881799767 92-93 15.337743686905133 32.38880739385892 31.868434656876765 20.40501426235918 94-95 15.178116848011506 32.50744012471066 31.176353262894903 21.13808976438293 96-97 14.696402605787956 31.63743641698907 30.45895254043328 23.207208436789696 98-99 15.739340474690191 31.218756563747114 30.595200588111744 22.446702373450954 100-101 17.04992058387262 31.032934721255955 30.880665782806737 21.036478912064688 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2332.0 1 1587.5 2 937.0 3 1049.5 4 943.0 5 1016.5 6 1793.0 7 3402.5 8 4938.0 9 5027.5 10 3777.5 11 2774.5 12 2605.5 13 2458.5 14 2090.5 15 1699.5 16 1402.0 17 1253.0 18 1166.0 19 1078.0 20 1057.0 21 1079.0 22 1108.0 23 1183.0 24 1242.5 25 1307.0 26 1445.5 27 1537.0 28 1607.0 29 1796.5 30 2115.0 31 2393.5 32 2757.5 33 3280.0 34 3638.0 35 3815.0 36 4186.5 37 4418.5 38 4357.0 39 4196.0 40 3912.5 41 3795.5 42 3630.0 43 3659.5 44 4083.5 45 4333.0 46 4569.5 47 5895.0 48 14390.5 49 14260.5 50 5634.0 51 3766.5 52 2772.5 53 2452.5 54 2301.5 55 2156.0 56 2059.0 57 2036.0 58 2056.5 59 2080.0 60 2173.5 61 2219.5 62 1876.0 63 1157.0 64 623.5 65 464.5 66 371.0 67 283.0 68 213.5 69 157.0 70 130.5 71 100.5 72 77.5 73 74.5 74 64.0 75 53.0 76 44.5 77 28.0 78 14.0 79 10.0 80 8.0 81 6.0 82 4.0 83 2.0 84 1.0 85 1.5 86 1.0 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06922229797052808 2 0.09858933347317636 3 0.07866170223923645 4 0.14316429807541034 5 0.1111752058314542 6 0.10697991504536158 7 0.12638313493103992 8 0.09596727673186847 9 0.12061461010016257 10-11 0.11432167392102364 12-13 0.09544286538360691 14-15 0.09806492212491479 16-17 0.11694373066233152 18-19 0.0933452199905606 20-21 0.07839949656510567 22-23 0.07708846819445173 24-25 0.0686978866222665 26-27 0.06135612774660444 28-29 0.07367979443075148 30-31 0.053227751848550005 32-33 0.056636425612250255 34-35 0.04614819864701872 36-37 0.05716083696051182 38-39 0.055849808589857886 40-41 0.07315538308248991 42-43 0.06738685825161256 44-45 0.05506319156746552 46-47 0.036446588704179564 48-49 0.049556872410718975 50-51 0.030153652525040642 52-53 0.04300173055744926 54-55 0.047983638365934236 56-57 0.049556872410718975 58-59 0.06450259583617389 60-61 0.06161833342073523 62-63 0.06345377313965074 64-65 0.041166290838533744 66-67 0.0686978866222665 68-69 0.05506319156746552 70-71 0.02779380145786355 72-73 0.019665425559809113 74-75 0.031202475221563795 76-77 0.0466726099952803 78-79 0.07027112066705124 80-81 0.04483717027636478 82-83 0.06397818448791233 84-85 0.06843568094813572 86-87 0.06764906392574335 88-89 0.09465624836121454 90-91 0.06922229797052808 92-93 0.08049714195815197 94-95 0.08862551785620641 96-97 0.1001625675179611 98-99 0.1311028370653941 100-101 0.1245476952121244 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 190690.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.633593790969634 #Duplication Level Percentage of deduplicated Percentage of total 1 77.83709316342346 33.18475011799255 2 11.794878102782356 10.057160836960511 3 3.977957637334252 5.087838900833814 4 1.9114861374203547 3.2597409407939586 5 1.032005707397476 2.199905605957313 6 0.6679131097935989 1.708532172636216 7 0.5141577898595292 1.5344276050133725 8 0.34564195921178875 1.1788767108920237 9 0.24108834165662132 0.9250616183334207 >10 1.468670816010234 11.145838795951544 >50 0.10947378779305765 3.196287167654308 >100 0.07134246844940835 6.130368661177828 >500 0.013530468154198134 3.8927054381456814 >1k 0.013530468154198134 13.230373905291309 >5k 0.001230042559472558 3.268131522366144 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6232 3.268131522366144 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4300 2.2549687975247785 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3824 2.005348995752268 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3258 1.708532172636216 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2792 1.4641564843463213 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2296 1.2040484556085795 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2242 1.175730242802454 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2158 1.131679689548482 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1187 0.6224762703864911 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1164 0.6104148093764749 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1007 0.5280822276994075 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1001 0.524935759609838 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 974 0.5107766532067755 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 833 0.4368346531018931 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 784 0.41113849703707583 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 671 0.35188001468351776 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 663 0.3476847238974251 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 645 0.33824531962871673 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 640 0.3356232628874089 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 636 0.3335256174943626 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 564 0.29576800041952905 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 510 0.26744978761340393 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 503 0.2637789081755729 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 495 0.25958361738948027 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 481 0.25224185851381825 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 443 0.23231422727987833 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 424 0.2223504116629084 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 374 0.19612984424982957 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 362 0.18983690807069065 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 360 0.1887880853741675 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 318 0.1667628087471813 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 297 0.1557501704336882 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 295 0.15470134773716504 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 281 0.14735958886150297 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 260 0.13634695054800985 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 246 0.12900519167234778 No Hit GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG 225 0.1179925533588547 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 225 0.1179925533588547 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 218 0.11432167392102364 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 209 0.10960197178666946 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 204 0.10697991504536158 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 200 0.10488226965231527 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 200 0.10488226965231527 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 198 0.10383344695579214 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 193 0.10121139021448423 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 192 0.10068697886622267 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 191 0.1001625675179611 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.622056741307882E-4 0.0 16-17 0.0 0.0 0.0 5.244113482615764E-4 0.0 18-19 0.0 0.0 0.0 7.866170223923645E-4 0.0 20-21 0.0 0.0 0.0 0.0010488226965231528 0.0 22-23 0.0 0.0 0.0 0.003933085111961823 0.0 24-25 0.0 0.0 0.0 0.007341758875662069 0.0 26-27 0.0 0.0 0.0 0.014159106403062564 0.0 28-29 0.0 0.0 0.0 0.02648277308720961 0.0 30-31 0.0 0.0 0.0 0.047983638365934236 0.0 32-33 0.0 0.0 0.0 0.06266715611725837 0.0 34-35 0.0 0.0 0.0 0.0778750852168441 0.0 36-37 0.0 0.0 0.0 0.09806492212491477 0.0 38-39 0.0 0.0 0.0 0.12743195762756307 0.0 40-41 0.0 0.0 0.0 0.16440295768000418 0.0 42-43 0.0 0.0 0.0 0.2199905605957313 0.0 44-45 0.0 0.0 0.0 0.26063244008600345 0.0 46-47 0.0 0.0 0.0 0.33011694373066236 0.0 48-49 0.0 0.0 0.0 0.42529760344013845 0.0 50-51 0.0 0.0 0.0 0.4622686034925796 0.0 52-53 0.0 0.0 0.0 0.5244113482615764 0.0 54-55 0.0 0.0 0.0 0.6119880434212597 0.0 56-57 0.0 0.0 0.0 0.7339136818920762 0.0 58-59 0.0 0.0 0.0 0.8828465047983638 0.0 60-61 0.0 0.0 0.0 1.083433845508417 0.0 62-63 0.0 0.0 0.0 1.269075462793015 0.0 64-65 0.0 0.0 0.0 1.4319051864282342 0.0 66-67 0.0 0.0 0.0 1.6497981016309193 0.0 68-69 0.0 0.0 0.0 1.9227542084010698 0.0 70-71 0.0 0.0 0.0 2.066180712150611 0.0 72-73 0.0 0.0 0.0 2.2701767266243644 0.0 74-75 0.0 0.0 0.0 2.485971996434003 0.0 76-77 0.0 0.0 0.0 2.6585033300120617 0.0 78-79 0.0 0.0 0.0 2.8638103728564683 0.0 80-81 0.0 0.0 0.0 3.2038911322041006 0.0 82-83 0.0 0.0 0.0 3.587498033457444 0.0 84-85 0.0 0.0 0.0 4.22465782159526 0.0 86-87 0.0 0.0 0.0 5.065027007184435 0.0 88-89 0.0 0.0 0.0 5.773244533011694 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCACT 25 5.2515672E-5 76.036354 1 GTCACTA 40 5.364126E-4 47.522724 2 AGATAGT 40 5.3710793E-4 47.510242 6 TAGTCAA 40 5.3780404E-4 47.497772 9 GATAGTC 40 5.3780404E-4 47.497772 7 TAGATAG 45 9.5946377E-4 42.231323 5 ACTACCT 50 0.0016107244 38.008194 5 TACCTGG 65 1.3203052E-4 36.55594 2 GGGGAGC 65 1.3243937E-4 36.536743 7 AAACGTG 55 0.0025715255 34.552902 6 GGGAGAC 55 0.0025748403 34.54383 7 CCAGGGA 85 1.6226935E-5 33.53664 4 CATGGGT 190 0.0 32.507008 4 ATGGGTT 60 0.0039385897 31.673498 5 TCTAGAT 75 3.0650024E-4 31.673494 3 CAGGGAG 75 3.0650024E-4 31.673494 5 CGACCGT 30 0.003948572 31.66518 18-19 GTTCGAC 30 0.003948572 31.66518 16-17 ATAGTCA 60 0.003943658 31.66518 8 ATGGGAG 485 0.0 31.346964 5 >>END_MODULE