##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936958_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 149311 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.96449022510063 33.0 31.0 34.0 30.0 34.0 2 31.979251361252686 33.0 31.0 34.0 30.0 34.0 3 31.89391940312502 33.0 31.0 34.0 30.0 34.0 4 35.55263845262573 37.0 35.0 37.0 33.0 37.0 5 35.586433685394915 37.0 35.0 37.0 33.0 37.0 6 35.744486340591116 37.0 35.0 37.0 35.0 37.0 7 35.72842590298102 37.0 35.0 37.0 35.0 37.0 8 35.683144577425644 37.0 35.0 37.0 35.0 37.0 9 37.25692011975005 39.0 37.0 39.0 34.0 39.0 10-11 37.12042649235488 39.0 37.0 39.0 34.0 39.0 12-13 37.039106294914646 39.0 37.0 39.0 33.5 39.0 14-15 37.89770345118578 40.0 37.5 41.0 33.0 41.0 16-17 37.92202182022758 40.0 37.5 41.0 33.0 41.0 18-19 38.108793056104375 40.0 38.0 41.0 33.5 41.0 20-21 38.15938209509011 40.0 38.0 41.0 34.0 41.0 22-23 38.06877925939817 40.0 37.5 41.0 34.0 41.0 24-25 37.850885065400405 40.0 37.0 41.0 33.0 41.0 26-27 37.70880578122174 40.0 36.5 41.0 33.0 41.0 28-29 37.52419446658317 39.5 36.0 41.0 33.0 41.0 30-31 37.14007005511985 39.0 35.5 41.0 33.0 41.0 32-33 36.62530557025269 38.0 35.0 41.0 33.0 41.0 34-35 36.29675643455606 38.0 35.0 40.5 32.0 41.0 36-37 35.94201699807783 38.0 35.0 40.0 31.0 41.0 38-39 35.57914688134163 38.0 35.0 40.0 30.5 41.0 40-41 35.18129608669153 37.0 35.0 40.0 30.0 41.0 42-43 34.772424670653876 37.0 35.0 40.0 28.0 41.0 44-45 34.174524984763345 37.0 35.0 40.0 24.0 41.0 46-47 33.360767123654654 36.5 33.5 40.0 17.0 41.0 48-49 32.68491939642759 36.0 33.0 40.0 4.5 41.0 50-51 31.711387640562315 35.5 32.0 39.0 2.0 40.5 52-53 31.78719920166632 35.0 33.0 39.5 2.0 40.5 54-55 32.05624836750139 35.0 33.0 40.0 2.0 41.0 56-57 31.898651137558517 35.0 33.0 40.0 2.0 41.0 58-59 31.647658243531957 35.0 33.0 40.0 2.0 41.0 60-61 31.29830688964644 35.0 32.5 39.5 2.0 41.0 62-63 30.873056908064378 35.0 31.5 39.0 2.0 41.0 64-65 30.40891494933394 35.0 31.0 39.0 2.0 41.0 66-67 29.966281787678067 35.0 30.5 38.5 2.0 40.0 68-69 29.589410693117053 35.0 30.0 37.5 2.0 40.0 70-71 29.060065902713127 35.0 29.0 37.0 2.0 39.5 72-73 28.583202175325326 35.0 28.5 36.5 2.0 39.0 74-75 28.145384465980406 35.0 27.5 36.0 2.0 39.0 76-77 27.636108525158896 34.5 26.5 35.0 2.0 37.5 78-79 27.184115704804064 34.0 25.5 35.0 2.0 37.0 80-81 26.762448848376877 34.0 24.5 35.0 2.0 36.5 82-83 26.324892338809597 34.0 21.5 35.0 2.0 36.0 84-85 25.93944518488256 34.0 20.0 35.0 2.0 36.0 86-87 25.529274467386863 34.0 15.5 35.0 2.0 35.0 88-89 25.0378237370321 33.0 4.5 35.0 2.0 35.0 90-91 24.54671122690224 33.0 2.0 35.0 2.0 35.0 92-93 24.187132897107382 33.0 2.0 35.0 2.0 35.0 94-95 23.758584431153764 33.0 2.0 35.0 2.0 35.0 96-97 23.199024184420438 32.5 2.0 35.0 2.0 35.0 98-99 22.593147859166436 32.0 2.0 35.0 2.0 35.0 100-101 20.93311276463221 29.5 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1254109098058045 1101 2 0.14926797416271853 1101 3 0.07852303713301723 1101 4 0.15670700990792596 1101 5 0.25634601322045114 1101 6 0.04694764400947804 1101 7 0.05447618840613444 1101 8 0.10827411727418479 1101 9 0.2212606515438722 1101 10-11 0.26084114272190817 1101 12-13 0.31170722939098994 1101 14-15 0.5688174447405814 1101 16-17 0.29452582471528643 1101 18-19 0.3140063405743021 1101 20-21 0.5481530340337315 1101 22-23 0.5831557839942789 1101 24-25 0.4711862665802542 1101 26-27 0.7522866203784915 1101 28-29 0.8284941435847628 1101 30-31 0.5354437623991544 1101 32-33 0.808689048889434 1101 34-35 0.8231216276709219 1101 36-37 1.002168794573727 1101 38-39 1.0127828689147762 1101 40-41 1.4028496995268043 1101 42-43 1.163581918395657 1101 44-45 1.650123075464954 1101 46-47 1.3785579837209099 1101 48-49 1.8941238588513158 1101 50-51 1.9859764561640425 1101 52-53 1.9642835190903796 1101 54-55 1.94707446316038 1101 56-57 1.9177199123241593 1101 58-59 1.9226050111361097 1101 60-61 2.1398280887998453 1101 62-63 2.0706623339188646 1101 64-65 2.401032657249239 1101 66-67 2.481783558588436 1101 68-69 2.3720985016156533 1101 70-71 2.423407158344151 1101 72-73 2.3451101465360438 1101 74-75 2.3743082556423794 1101 76-77 2.1547388380556463 1101 78-79 2.329393236056845 1101 80-81 2.353490759800298 1101 82-83 2.408638440617242 1101 84-85 2.6262918152415793 1101 86-87 2.6341127672983013 1101 88-89 2.563010045444834 1101 90-91 2.6571966416030364 1101 92-93 2.4152168976007182 1101 94-95 2.4961251977525514 1101 96-97 3.011184878166077 1101 98-99 3.043471028703987 1101 100-101 2.7498756241093787 1102 1 0.4573137895347763 1102 2 0.32379406918570197 1102 3 0.31753247490021863 1102 4 0.5059527951785512 1102 5 0.3288635577876846 1102 6 0.031814950615917326 1102 7 0.27620725237322574 1102 8 0.11419490456142967 1102 9 0.5516793012171775 1102 10-11 0.4340040047046472 1102 12-13 0.3893792468145634 1102 14-15 0.5333895186294271 1102 16-17 0.5621792892631632 1102 18-19 0.4210241674679054 1102 20-21 0.4723685700505058 1102 22-23 0.05366477810576242 1102 24-25 0.696910232055771 1102 26-27 0.3950953644294728 1102 28-29 0.38541113963219686 1102 30-31 0.507025596713909 1102 32-33 0.5833522738632198 1102 34-35 0.44606266179207665 1102 36-37 0.4753719888949419 1102 38-39 0.6514051162326737 1102 40-41 0.18069917923963885 1102 42-43 0.40410785208928957 1102 44-45 0.2973441773444492 1102 46-47 0.6033462389439492 1102 48-49 1.2723040637395897 1102 50-51 1.5820561528648547 1102 52-53 1.5784966351668501 1102 54-55 1.6669905718963598 1102 56-57 1.5174537188902661 1102 58-59 1.6158614441440982 1102 60-61 1.7201217082238998 1102 62-63 1.7367347707987513 1102 64-65 1.9945310842880346 1102 66-67 2.079024826637159 1102 68-69 1.8535940247260818 1102 70-71 2.494311409372628 1102 72-73 2.6682770417526243 1102 74-75 2.5196256711461267 1102 76-77 2.6087438392656175 1102 78-79 2.9383631481323462 1102 80-81 3.278682338408025 1102 82-83 2.751132592428167 1102 84-85 2.426864534447841 1102 86-87 2.1776071611032535 1102 88-89 2.2387057782834248 1102 90-91 2.5296093220902485 1102 92-93 2.5856048946967896 1102 94-95 2.382759060783929 1102 96-97 1.8777735689783732 1102 98-99 1.6709373512013705 1102 100-101 1.6891189894397023 1103 1 -0.15279790946581429 1103 2 0.14494129350753582 1103 3 0.14620468452481106 1103 4 -0.012196192811707363 1103 5 0.10596946187772005 1103 6 0.09577732568940434 1103 7 -0.052910073569499616 1103 8 -0.023152874056187045 1103 9 -0.3715759267999559 1103 10-11 -0.022466978750465216 1103 12-13 -0.20408678157210147 1103 14-15 0.015937599590209572 1103 16-17 -0.025046712248560254 1103 18-19 -0.17817798252789885 1103 20-21 0.3245563587462357 1103 22-23 0.1385812138158471 1103 24-25 0.29115731360443675 1103 26-27 0.28823385439336136 1103 28-29 0.10804488448816585 1103 30-31 0.0958221029707289 1103 32-33 0.5941442449726466 1103 34-35 0.5125277663524201 1103 36-37 0.2369156186925352 1103 38-39 0.35981193575060644 1103 40-41 0.2854274903171543 1103 42-43 -0.09810093265917885 1103 44-45 0.20860646003530547 1103 46-47 0.3804773382972684 1103 48-49 0.7045875944358215 1103 50-51 0.5972908887345092 1103 52-53 0.940015069701154 1103 54-55 0.9099316989644741 1103 56-57 1.1586395759491381 1103 58-59 1.2309786021184017 1103 60-61 1.2605685042262564 1103 62-63 1.514529345418726 1103 64-65 1.869176250996695 1103 66-67 2.2015577904399635 1103 68-69 2.0267610693216973 1103 70-71 2.005565293642384 1103 72-73 2.2302741862894564 1103 74-75 2.2534377144819793 1103 76-77 2.2356607788809697 1103 78-79 2.542343999732246 1103 80-81 2.523613891276085 1103 82-83 2.3202180043707195 1103 84-85 2.170737886257701 1103 86-87 1.935841812988702 1103 88-89 1.9773385153288565 1103 90-91 1.7520583016820872 1103 92-93 1.53170382809731 1103 94-95 1.5415228131984762 1103 96-97 1.6717519010519446 1103 98-99 1.6043210750612822 1103 100-101 1.4119878900863796 1104 1 0.2207689390364358 1104 2 0.17998770943250264 1104 3 0.3152543543496691 1104 4 0.21919437087859706 1104 5 0.09421714108147228 1104 6 0.023177076861763624 1104 7 0.33372979627450405 1104 8 0.15776396009061955 1104 9 0.13649183088020322 1104 10-11 0.32313547124474695 1104 12-13 0.5048040213755485 1104 14-15 0.8142627981738073 1104 16-17 0.750836147355237 1104 18-19 0.7485039966088607 1104 20-21 0.6501568946826879 1104 22-23 0.560736443981412 1104 24-25 0.7458898238925045 1104 26-27 0.5857455780032765 1104 28-29 0.4993646280042867 1104 30-31 0.8798779934865735 1104 32-33 0.8430580166254416 1104 34-35 0.47055245771044696 1104 36-37 0.3908442243007286 1104 38-39 0.6125346843389892 1104 40-41 0.3329535693677812 1104 42-43 0.9782891525164317 1104 44-45 1.3161861327312536 1104 46-47 1.8691269698409627 1104 48-49 1.0707853167058943 1104 50-51 0.8542915586550812 1104 52-53 0.8656347462252256 1104 54-55 1.020573865678987 1104 56-57 0.6622092955395331 1104 58-59 0.5628476804991074 1104 60-61 0.6244406451009787 1104 62-63 1.097484652146644 1104 64-65 1.387934026860414 1104 66-67 1.2680139106428534 1104 68-69 1.4405422924052438 1104 70-71 1.5106854008296757 1104 72-73 1.3937635154118588 1104 74-75 1.792857754677211 1104 76-77 1.9418240480933342 1104 78-79 2.1337594461864526 1104 80-81 1.9683358742409638 1104 82-83 1.703766669314735 1104 84-85 1.9450894988522052 1104 86-87 1.350934166323949 1104 88-89 1.7518049714490616 1104 90-91 1.7171745807518555 1104 92-93 1.7299333237428236 1104 94-95 1.6720329098584443 1104 96-97 1.5198713383186657 1104 98-99 1.6276055998961958 1104 100-101 1.0233382585426902 1105 1 -0.13573640351541982 1105 2 -0.24829638861903902 1105 3 -0.05533018367672682 1105 4 -0.4740802991402546 1105 5 -0.24896324861055064 1105 6 -0.2261001072991391 1105 7 -0.43333494288325625 1105 8 -0.3304824258301835 1105 9 -0.2619996838903802 1105 10-11 -0.32909582982376406 1105 12-13 -0.6342280339355781 1105 14-15 -0.4867428586458047 1105 16-17 -0.2398615270633755 1105 18-19 -0.33672541885310636 1105 20-21 -0.20898829702064603 1105 22-23 -0.6082767552643489 1105 24-25 -0.7676236896210114 1105 26-27 -0.5374473823989874 1105 28-29 -0.44702129280022973 1105 30-31 -0.27229045403070984 1105 32-33 -0.3076019456624337 1105 34-35 0.13812002527801326 1105 36-37 0.18701015767596374 1105 38-39 0.3043008691284328 1105 40-41 0.04539857251046442 1105 42-43 0.2251779017307669 1105 44-45 0.6060038574327038 1105 46-47 0.4373230729207833 1105 48-49 0.2444496787423489 1105 50-51 -0.0689642552984111 1105 52-53 0.4945473797890223 1105 54-55 0.6455895790667974 1105 56-57 0.9268855997507188 1105 58-59 0.6710186421584403 1105 60-61 -0.023893735791538973 1105 62-63 0.2752344950127643 1105 64-65 0.6008190048616697 1105 66-67 0.7777562110828278 1105 68-69 0.5827170252776561 1105 70-71 0.8215590651917104 1105 72-73 1.2491374940416513 1105 74-75 1.3168678112454124 1105 76-77 1.142389730054365 1105 78-79 1.1898248138797278 1105 80-81 1.2924716379116106 1105 82-83 0.9842820684347835 1105 84-85 0.7935495868471776 1105 86-87 1.1696645245891908 1105 88-89 1.047530929965717 1105 90-91 0.7547610043847897 1105 92-93 0.948098000047036 1105 94-95 1.6226928721463132 1105 96-97 1.729676492309867 1105 98-99 2.040490577897451 1105 100-101 2.114601614922325 1106 1 0.25156569147898367 1106 2 0.16091518982330655 1106 3 -0.16791614241653008 1106 4 0.0764394513794926 1106 5 -0.08062804950611735 1106 6 0.02369998865479772 1106 7 0.246210875124099 1106 8 0.14810385275609406 1106 9 -0.31423061304438704 1106 10-11 0.028364589175119193 1106 12-13 -0.001539724324189251 1106 14-15 0.11829472331485391 1106 16-17 0.27848166820290743 1106 18-19 0.2521919008334379 1106 20-21 0.17415579381574986 1106 22-23 -0.1320253559360225 1106 24-25 0.16600816708777444 1106 26-27 0.01782208825509457 1106 28-29 -0.04772495504235508 1106 30-31 0.003753045089709417 1106 32-33 -0.001540854985954354 1106 34-35 0.28475364114240165 1106 36-37 0.641607950735299 1106 38-39 1.053349325869199 1106 40-41 1.0923040578774916 1106 42-43 0.7715876250635674 1106 44-45 0.2180300848435408 1106 46-47 0.13605967934969954 1106 48-49 0.5812160308933159 1106 50-51 0.6570712476601699 1106 52-53 1.254598555224817 1106 54-55 1.2576317988013166 1106 56-57 0.9328987134561402 1106 58-59 1.3643338508582623 1106 60-61 1.5129365856541597 1106 62-63 1.5985992799160123 1106 64-65 1.6008829741564128 1106 66-67 1.868784517495751 1106 68-69 1.5460466270687938 1106 70-71 1.5707514528456343 1106 72-73 2.286511554492634 1106 74-75 1.6735925833548002 1106 76-77 2.0581581612074125 1106 78-79 1.4126778444250654 1106 80-81 1.2561850554711853 1106 82-83 0.9874325562006554 1106 84-85 0.9737670824492888 1106 86-87 0.8494342351281325 1106 88-89 1.1501536710499956 1106 90-91 0.929573094581496 1106 92-93 1.0813300486637019 1106 94-95 0.6314246808781228 1106 96-97 -0.10686596730951692 1106 98-99 -0.022167346298932245 1106 100-101 0.2244528863120614 1107 1 -0.25827062604964013 1107 2 0.09336735304470523 1107 3 -0.09341372176026397 1107 4 -0.311672436732394 1107 5 -0.271321970969268 1107 6 -0.4214318026308348 1107 7 -0.22305063259894808 1107 8 -0.1525954479432059 1107 9 -0.026057247947946394 1107 10-11 -0.3028234535513832 1107 12-13 -0.20904375875129944 1107 14-15 -0.3689295908325363 1107 16-17 -0.4632381504399987 1107 18-19 -0.0421483742760671 1107 20-21 -0.5781880162203592 1107 22-23 -0.39030553729312345 1107 24-25 0.21666904467172543 1107 26-27 0.21967792472631942 1107 28-29 0.017229321450379587 1107 30-31 0.06240593066566902 1107 32-33 -0.2311189691794553 1107 34-35 0.07509791225589879 1107 36-37 -0.2599712393054361 1107 38-39 -0.21223124740368604 1107 40-41 0.2094561365680292 1107 42-43 0.13351416006702266 1107 44-45 0.8094963109251481 1107 46-47 1.022826758424472 1107 48-49 1.0647901490828175 1107 50-51 1.6563604200262638 1107 52-53 1.5728380080796498 1107 54-55 1.656137139614355 1107 56-57 1.4575279303930486 1107 58-59 1.6096462307860229 1107 60-61 1.8512905393927355 1107 62-63 1.754174473952741 1107 64-65 1.2478706019132701 1107 66-67 1.515945049271668 1107 68-69 1.6157461583067878 1107 70-71 1.7957956394282846 1107 72-73 1.6064032013073586 1107 74-75 1.462111756489353 1107 76-77 1.8701538578184902 1107 78-79 0.9702740943290031 1107 80-81 0.5227454181853908 1107 82-83 0.3897225738752894 1107 84-85 0.54625228954988 1107 86-87 0.9546943596190225 1107 88-89 1.1766464090811937 1107 90-91 1.0799803796041623 1107 92-93 1.2514676034166392 1107 94-95 1.528028077481519 1107 96-97 2.0230425856759595 1107 98-99 1.2648149022217758 1107 100-101 1.272885223205936 1108 1 0.046411533895675205 1108 2 -0.11664631749528453 1108 3 -0.0042988892243798205 1108 4 0.1518443096790918 1108 5 -0.3131879996774032 1108 6 -0.3185609892337098 1108 7 0.17185709125614324 1108 8 0.1440552697601447 1108 9 0.08245266319354982 1108 10-11 -0.07187676865733295 1108 12-13 0.22247538000468126 1108 14-15 0.4597187345263194 1108 16-17 0.25882268346294524 1108 18-19 0.44448012879981036 1108 20-21 0.36905224351989574 1108 22-23 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2316 90-91 -1.6608204861967018 2316 92-93 -1.164657937708899 2316 94-95 -1.702491403037964 2316 96-97 -0.9542301415967671 2316 98-99 -1.3185184311115563 2316 100-101 -1.601573651252938 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 426.0 3 118.0 4 110.0 5 89.0 6 103.0 7 144.0 8 194.0 9 225.0 10 360.0 11 508.0 12 852.0 13 1211.0 14 1453.0 15 2051.0 16 2720.0 17 3848.0 18 3222.0 19 1875.0 20 1661.0 21 1694.0 22 1777.0 23 1957.0 24 1982.0 25 2039.0 26 2192.0 27 2489.0 28 2846.0 29 3137.0 30 3568.0 31 4377.0 32 5406.0 33 6790.0 34 9972.0 35 17406.0 36 19191.0 37 21120.0 38 17083.0 39 3112.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.57522645655786 19.242107519965696 15.908908184595594 15.273757838880849 2 14.710887772194306 24.14606365159129 40.45159128978224 20.69145728643216 3 19.438657601232542 33.61020866128546 32.314030210670865 14.637103526811131 4 10.531499360164013 19.322376839947207 38.99046610879221 31.155657691096568 5 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17.545528221134205 32.833566639652254 32.82050595098558 16.800399188227967 52-53 15.909928132723389 33.15003717272928 33.17448410279767 17.765550591749662 54-55 15.043050471696533 32.529478940265165 33.87608295964876 18.551387628389534 56-57 16.394579241415347 34.18119213815834 30.904754886855397 18.51947373357092 58-59 15.827316445861408 32.37197090460945 32.29963429826794 19.501078351261203 60-61 16.403523461834745 31.45761462973507 33.185517634055664 18.953344274374516 62-63 18.981321776524453 31.193671441441744 32.49850125425761 17.326505527776195 64-65 17.992993831088366 31.539146801342287 33.893849173124714 16.57401019444463 66-67 16.582714296240226 31.85657541311934 33.908273104205875 17.652437186434547 68-69 16.193946864239877 33.45469526654966 33.09027452136283 17.261083347847638 70-71 16.680454565245785 32.853492134199236 31.550954379360352 18.915098921194623 72-73 15.964619079037709 32.18612159595953 31.398055456009967 20.451203868992796 74-75 16.13971745697884 32.58957578355785 32.4686677339621 18.802039025501216 76-77 17.048663096230946 31.03264637614172 32.893560153133514 19.025130374493827 78-79 15.512216691343466 30.935663895272103 34.13471523418396 19.41740417920047 80-81 15.512644448166135 32.97672081728354 32.45687489532742 19.053759839222913 82-83 15.38953644158628 31.950696677384784 31.815715434083604 20.84405144694534 84-85 15.548313846586664 30.28444733219684 31.78086871481249 22.386370106403998 86-87 16.49835371511065 30.490937896707766 32.304363408596856 20.706344979584728 88-89 17.387081432657464 28.763338223177865 32.52449946077742 21.32508088338726 90-91 15.340871032995299 29.254692084075717 33.9718943828955 21.43254250003349 92-93 14.93099752127018 31.01125477322972 33.00596235010384 21.051785355396262 94-95 14.869123976353556 31.27962085308057 32.0553313348852 21.79592383568067 96-97 14.56092454568294 30.387739720291435 31.08617368729587 23.965162046729755 98-99 15.606888002948171 30.210727327548664 31.134041341418474 23.04834332808469 100-101 16.968324275787204 29.807814730183406 31.36747726678773 21.85638372724166 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1622.0 1 1147.0 2 730.5 3 780.5 4 687.0 5 748.0 6 1339.0 7 2578.5 8 3861.0 9 4007.5 10 2941.0 11 2031.0 12 1882.0 13 1829.5 14 1588.0 15 1350.0 16 1146.5 17 1011.5 18 951.0 19 914.0 20 901.5 21 892.5 22 930.5 23 1000.0 24 1064.0 25 1101.5 26 1160.0 27 1215.5 28 1305.5 29 1502.5 30 1724.0 31 1925.0 32 2157.5 33 2546.0 34 2963.0 35 3134.5 36 3346.5 37 3531.0 38 3478.0 39 3276.0 40 3014.5 41 3015.5 42 2889.5 43 2859.5 44 3168.5 45 3371.0 46 3692.5 47 4852.0 48 11462.5 49 11217.5 50 4352.0 51 2879.5 52 2138.0 53 1877.0 54 1776.0 55 1642.5 56 1560.0 57 1558.0 58 1497.0 59 1482.0 60 1564.5 61 1570.5 62 1332.5 63 845.5 64 474.5 65 383.0 66 301.0 67 214.0 68 172.0 69 125.5 70 99.0 71 80.5 72 69.0 73 80.5 74 61.5 75 38.0 76 31.0 77 15.5 78 14.0 79 12.5 80 8.5 81 6.5 82 5.5 83 3.5 84 1.5 85 1.5 86 1.0 87 0.5 88 1.5 89 1.0 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0368358660781858 2 0.04085432419580607 3 0.01741331850968783 4 0.036166123058582425 5 0.0522399555290635 6 0.05558867062708039 7 0.06027687176430403 8 0.05893738572509728 9 0.05893738572509728 10-11 0.0676440449799412 12-13 0.06362558686232092 14-15 0.06931840252894964 16-17 0.05023072647025337 18-19 0.044537910803624646 20-21 0.04822149741144323 22-23 0.02645484927433344 24-25 0.012725117372464184 26-27 0.012055374352860807 28-29 0.016073832470481077 30-31 0.01105075982345574 32-33 0.006697430196033782 34-35 0.0077020447254388495 36-37 0.005357944156827025 38-39 0.00736717321563716 40-41 0.008371787745042226 42-43 0.006027687176430404 44-45 0.0016743575490084455 46-47 0.0026789720784135126 48-49 0.0010046145294050673 50-51 0.005357944156827025 52-53 0.005357944156827025 54-55 0.006362558686232092 56-57 0.02210151964691148 58-59 0.006027687176430404 60-61 0.01741331850968783 62-63 0.013059988882265875 64-65 0.009376402274447295 66-67 0.013394860392067564 68-69 0.02210151964691148 70-71 0.017078446999886143 72-73 0.01439947492147263 74-75 0.016073832470481077 76-77 0.019757419078299657 78-79 0.032147664940962155 80-81 0.024110748705721614 82-83 0.020762033607704723 84-85 0.01439947492147263 86-87 0.023775877195919923 88-89 0.01473434643127432 90-91 0.011385631333257429 92-93 0.027459463803738503 94-95 0.01908767605869628 96-97 0.03248253645076384 98-99 0.04420303929382296 100-101 0.053579441568270256 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 149311.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.414008344998024 #Duplication Level Percentage of deduplicated Percentage of total 1 79.33197617431954 35.234510518314124 2 11.205609590590363 9.953720757345407 3 3.5738520696674962 4.761872869380019 4 1.8381964864661087 3.265666963586072 5 1.0223931237276633 2.2704288364554523 6 0.6182613285078791 1.6475678282243105 7 0.43881474779461666 1.3642665309320814 8 0.28952725627686043 1.028725278110789 9 0.272939757219332 1.091011378933903 >10 1.221443112418005 9.947023327149372 >50 0.07992158636809168 2.4767096864932925 >100 0.08293749528764231 7.821928725947854 >500 0.009047726758651889 2.854444749549598 >1k 0.013571590137977834 12.848350088071205 >5k 0.0015079544597753148 3.43377246150652 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5127 3.43377246150652 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3652 2.4459015075915373 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3045 2.0393674946922866 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2783 1.8638948235562014 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2277 1.5250048556368923 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1914 1.2818881395208659 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1804 1.208216407364494 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1698 1.1372236472865362 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1010 0.676440449799412 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1001 0.6704127626229816 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 855 0.5726302817608884 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 837 0.5605749074080275 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 804 0.5384733877611161 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 637 0.4266263034873519 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 585 0.39179966646797626 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 544 0.3643402026642377 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 486 0.3254951075272418 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 473 0.3167884482723979 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 462 0.3094212750567607 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 461 0.30875153203715733 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 443 0.2966961576842965 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 432 0.2893289844686594 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 395 0.26454849274333436 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 391 0.26186952066492086 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 385 0.2578510625473006 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 367 0.2457956881944398 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 335 0.2243639115671317 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 318 0.21297828023387425 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 283 0.18953727454775604 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 264 0.17681215717529183 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 263 0.17614241415568846 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 252 0.1687752409400513 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 247 0.1654265258420344 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 225 0.1506921794107601 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 217 0.14533423525393308 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 204 0.13662757599908915 No Hit GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG 189 0.12658143070503847 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 180 0.12055374352860806 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 179 0.11988400050900469 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 178 0.11921425748940133 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 170 0.1138563133325743 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 168 0.11251682729336754 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 164 0.10983785521495401 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 154 0.10314042501892023 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 153 0.10247068199931686 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 3.348715098016891E-4 0.0 18-19 0.0 0.0 0.0 6.697430196033782E-4 0.0 20-21 0.0 0.0 0.0 0.0010046145294050671 0.0 22-23 0.0 0.0 0.0 0.003348715098016891 0.0 24-25 0.0 0.0 0.0 0.008371787745042228 0.0 26-27 0.0 0.0 0.0 0.020762033607704726 0.0 28-29 0.0 0.0 0.0 0.036166123058582425 0.0 30-31 0.0 0.0 0.0 0.064630201391726 0.0 32-33 0.0 0.0 0.0 0.08103890537200875 0.0 34-35 0.0 0.0 0.0 0.10012658143070505 0.0 36-37 0.0 0.0 0.0 0.12457220164622834 0.0 38-39 0.0 0.0 0.0 0.15203166544996685 0.0 40-41 0.0 0.0 0.0 0.19020701756735942 0.0 42-43 0.0 0.0 0.0 0.24144235856701782 0.0 44-45 0.0 0.0 0.0 0.2762689955863935 0.0 46-47 0.0 0.0 0.0 0.35362431435058367 0.0 48-49 0.0 0.0 0.0 0.4604483259773225 0.0 50-51 0.0 0.0 0.0 0.5043164937613438 0.0 52-53 0.0 0.0 0.0 0.5706210527020782 0.0 54-55 0.0 0.0 0.0 0.681798393956239 0.0 56-57 0.0 0.0 0.0 0.8532526069747037 0.0 58-59 0.0 0.0 0.0 1.0357575798166243 0.0 60-61 0.0 0.0 0.0 1.282892754050271 0.0 62-63 0.0 0.0 0.0 1.5029033359899806 0.0 64-65 0.0 0.0 0.0 1.6894267669495213 0.0 66-67 0.0 0.0 0.0 1.9640214049869065 0.0 68-69 0.0 0.0 0.0 2.2690893504162455 0.0 70-71 0.0 0.0 0.0 2.4341810047484778 0.0 72-73 0.0 0.0 0.0 2.676293106335099 0.0 74-75 0.0 0.0 0.0 2.9244328950981506 0.0 76-77 0.0 0.0 0.0 3.1327229741948015 0.0 78-79 0.0 0.0 0.0 3.376509433330431 0.0 80-81 0.0 0.0 0.0 3.7987824071903606 0.0 82-83 0.0 0.0 0.0 4.270951236010743 0.0 84-85 0.0 0.0 0.0 5.026086490613551 0.0 86-87 0.0 0.0 0.0 6.097340450469154 0.0 88-89 0.0 0.0 0.0 6.953272029522273 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCACT 25 0.00500973 57.008648 1 AGGCTTA 25 0.00500973 57.008648 6 ACTATAT 35 2.7779854E-4 54.29395 8 CCCTATA 35 2.7779854E-4 54.29395 2 ATACTAT 40 5.3690095E-4 47.507206 6 ATGGGGT 65 2.5714926E-6 43.852806 5 GGACCAT 45 9.5909636E-4 42.228626 6 AGAGCTC 25 0.0016153543 37.99303 32-33 CCCCTAT 55 0.0025705548 34.550694 1 TACTATA 55 0.0025705548 34.550694 7 ATTAGCC 70 2.0429882E-4 33.93372 3 GGGATTG 70 2.0429882E-4 33.93372 7 CTATACT 80 4.4711537E-4 29.692003 4 GATGTAA 65 0.0058236816 29.235205 9 TGGGATT 235 0.0 28.302164 6 TACCAGA 35 0.008375592 27.146975 20-21 GGATGTC 35 0.008375592 27.146975 14-15 AGAACTC 35 0.008375592 27.146975 24-25 TCGCTGC 35 0.008375592 27.146975 16-17 CCTATAC 70 0.008362405 27.146975 3 >>END_MODULE