##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936958_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 149311 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.083697785159835 31.0 31.0 34.0 31.0 34.0 2 32.0952441548178 33.0 31.0 34.0 30.0 34.0 3 32.13665436571987 33.0 31.0 34.0 30.0 34.0 4 35.90128657634066 37.0 35.0 37.0 35.0 37.0 5 35.85410987803979 37.0 35.0 37.0 35.0 37.0 6 35.98432801334128 37.0 35.0 37.0 35.0 37.0 7 35.94560347194781 37.0 35.0 37.0 35.0 37.0 8 35.95993597256732 37.0 35.0 37.0 35.0 37.0 9 37.548472651043795 39.0 37.0 39.0 35.0 39.0 10-11 37.47076571719431 39.0 37.0 39.0 35.0 39.0 12-13 37.34598589521201 39.0 37.0 39.0 34.0 39.0 14-15 38.1719096382718 40.0 37.0 41.0 33.0 41.0 16-17 38.17169532050552 40.0 37.0 41.0 33.5 41.0 18-19 38.390550595736414 40.0 37.5 41.0 34.0 41.0 20-21 38.54404230096912 40.0 38.0 41.0 34.0 41.0 22-23 38.447227598770354 40.0 37.5 41.0 34.5 41.0 24-25 38.281489642424205 40.0 37.0 41.0 34.0 41.0 26-27 37.90528159345259 40.0 36.5 41.0 33.5 41.0 28-29 37.61447917434081 39.5 35.5 41.0 33.0 41.0 30-31 37.20250684812237 39.0 35.0 41.0 33.0 41.0 32-33 36.74702466663541 38.0 35.0 41.0 33.0 41.0 34-35 36.44280394612588 38.0 35.0 41.0 32.5 41.0 36-37 36.07224183080952 38.0 35.0 40.0 31.5 41.0 38-39 35.72928317404612 38.0 35.0 40.0 31.0 41.0 40-41 35.30075480038309 37.0 35.0 40.0 30.0 41.0 42-43 34.84685321242239 37.0 35.0 40.0 27.5 41.0 44-45 34.26493359497961 37.0 34.5 40.0 23.5 41.0 46-47 33.36549216065795 37.0 33.0 40.0 17.5 41.0 48-49 32.59797000890758 36.0 33.0 40.0 4.5 41.0 50-51 32.241522727729375 36.0 33.0 40.0 2.0 41.0 52-53 31.878501918813754 35.0 33.0 40.0 2.0 41.0 54-55 31.594872447441915 35.0 32.0 40.0 2.0 41.0 56-57 31.339690310827734 35.0 32.0 39.5 2.0 41.0 58-59 31.016914359960083 35.0 31.0 39.0 2.0 41.0 60-61 30.596272210352886 35.0 31.0 39.0 2.0 40.5 62-63 30.16392630147812 35.0 30.0 38.5 2.0 40.0 64-65 29.61778100742745 35.0 29.0 38.0 2.0 40.0 66-67 29.133198491738717 35.0 28.5 37.0 2.0 40.0 68-69 28.672502360844145 35.0 27.5 37.0 2.0 39.0 70-71 28.23349250892433 34.0 26.0 36.0 2.0 39.0 72-73 27.844602875876525 34.0 26.0 35.5 2.0 38.5 74-75 27.372434047056146 34.0 26.0 35.0 2.0 37.5 76-77 25.892767445131305 32.0 23.5 34.5 2.0 36.0 78-79 26.44716397318349 33.0 24.0 35.0 2.0 36.0 80-81 26.088848108980585 33.0 20.0 35.0 2.0 36.0 82-83 25.90923977469845 33.0 20.0 35.0 2.0 36.0 84-85 25.573296676065393 33.0 19.0 35.0 2.0 35.0 86-87 25.10572898178969 33.0 11.5 35.0 2.0 35.0 88-89 24.57437161361186 33.0 3.5 35.0 2.0 35.0 90-91 23.984629397700104 32.0 2.0 35.0 2.0 35.0 92-93 23.577874369604384 32.0 2.0 35.0 2.0 35.0 94-95 23.05389756950258 31.5 2.0 34.5 2.0 35.0 96-97 22.332021753253276 31.0 2.0 34.0 2.0 35.0 98-99 21.611746622820824 31.0 2.0 34.0 2.0 35.0 100-101 19.119274534361168 26.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.11794492381945076 1101 2 0.22952108132109572 1101 3 0.24290578509523897 1101 4 0.1868490659460349 1101 5 0.35370792410834895 1101 6 0.3450321032632857 1101 7 0.3165027109866543 1101 8 0.34917590084939576 1101 9 0.52767809535532 1101 10-11 0.3875490940456956 1101 12-13 0.33537863302887416 1101 14-15 0.7636982833664163 1101 16-17 0.3738634280927329 1101 18-19 0.48447675994047046 1101 20-21 0.45851015517835236 1101 22-23 0.35464889758814166 1101 24-25 0.48496688136782495 1101 26-27 0.7121786961444485 1101 28-29 0.5973660882439518 1101 30-31 0.4834400050889869 1101 32-33 0.9727756939246675 1101 34-35 0.8429656030284249 1101 36-37 0.7745344412709301 1101 38-39 1.0654509874780942 1101 40-41 1.4071979150386014 1101 42-43 1.3521536898830604 1101 44-45 1.8822418959415401 1101 46-47 1.7940914555407872 1101 48-49 1.4849838638558381 1101 50-51 1.2205440997410726 1101 52-53 1.674645055381358 1101 54-55 1.8990797104761015 1101 56-57 1.3610607118471627 1101 58-59 1.8421765991321557 1101 60-61 2.0797937250620535 1101 62-63 2.0623104659469647 1101 64-65 2.2617996893398455 1101 66-67 2.137536076352742 1101 68-69 1.9490367900819017 1101 70-71 2.2351527346582927 1101 72-73 1.8393684314862817 1101 74-75 1.950176577689966 1101 76-77 1.6689607317971422 1101 78-79 1.9860069675637142 1101 80-81 2.3786563069550404 1101 82-83 2.2917709961508734 1101 84-85 1.889632675240641 1101 86-87 2.0388211779008465 1101 88-89 2.0886363576952753 1101 90-91 2.1841560362540307 1101 92-93 2.419099114158069 1101 94-95 2.3571276472389613 1101 96-97 2.5868089161875325 1101 98-99 2.727225103368326 1101 100-101 2.8520908695047176 1102 1 0.32362371707588977 1102 2 0.08371161128802385 1102 3 0.029773575689738152 1102 4 0.10423230382616566 1102 5 -0.0328372077783996 1102 6 0.32167161282205115 1102 7 0.19311802114233245 1102 8 0.22325834014922918 1102 9 0.03891468485277727 1102 10-11 0.3731262498091894 1102 12-13 0.040476640611352366 1102 14-15 0.3198247655798525 1102 16-17 0.04380776946564424 1102 18-19 -0.017443059370656044 1102 20-21 0.11812135143550506 1102 22-23 0.25047046732274936 1102 24-25 0.46495397501951885 1102 26-27 0.25267236396731363 1102 28-29 0.7024156970202711 1102 30-31 0.5747604858504616 1102 32-33 0.09874971989869152 1102 34-35 0.20453695093517865 1102 36-37 0.14446264970845846 1102 38-39 0.017802362810833472 1102 40-41 0.7761097485467161 1102 42-43 0.446547494835869 1102 44-45 0.7938818088236914 1102 46-47 1.1350933108283527 1102 48-49 1.0350040300250711 1102 50-51 0.7486880055247305 1102 52-53 1.115445248976581 1102 54-55 1.1881496467510146 1102 56-57 1.0586246386036358 1102 58-59 1.0066841412794538 1102 60-61 1.0858839081708709 1102 62-63 0.8924629221863789 1102 64-65 0.8993652293898542 1102 66-67 1.1796511041820565 1102 68-69 0.8694691527502876 1102 70-71 1.2481881464514686 1102 72-73 1.3456683427551361 1102 74-75 1.7514107472471174 1102 76-77 1.781826747768747 1102 78-79 1.9060440733151083 1102 80-81 1.6367429408180705 1102 82-83 1.706274505064318 1102 84-85 1.5010951258335226 1102 86-87 1.6355542584848592 1102 88-89 2.0272182726747054 1102 90-91 1.783058996647675 1102 92-93 1.840338123595835 1102 94-95 2.3546673745923528 1102 96-97 2.2439840392011625 1102 98-99 2.3550544169119263 1102 100-101 1.9102581680446846 1103 1 -0.07654425134604992 1103 2 -0.05506389891606034 1103 3 -0.12138120721107981 1103 4 0.09610263220442761 1103 5 0.17107645645272385 1103 6 -0.09593147779691691 1103 7 5.086317075466695E-4 1103 8 0.05159461292994649 1103 9 -0.10888072960930373 1103 10-11 -0.13268545758982242 1103 12-13 -0.09029517428744072 1103 14-15 -0.0019859340086227917 1103 16-17 -0.10948861081486427 1103 18-19 -0.13831313579628812 1103 20-21 0.005859656634129351 1103 22-23 0.11790139030923541 1103 24-25 0.04462139754408412 1103 26-27 -0.01250778216521553 1103 28-29 -0.049562131148036315 1103 30-31 0.03628731880586855 1103 32-33 -0.17967473630354647 1103 34-35 -0.08200877846768861 1103 36-37 0.21811680272769252 1103 38-39 -0.009517701017166758 1103 40-41 0.5233676640585969 1103 42-43 0.20465617040485995 1103 44-45 0.37684981083931035 1103 46-47 0.4759096373589671 1103 48-49 0.6298138057872293 1103 50-51 0.8542645571330247 1103 52-53 0.8340897438115569 1103 54-55 0.35186846761268953 1103 56-57 0.31786710102069904 1103 58-59 0.5669932031758158 1103 60-61 0.6147324846384947 1103 62-63 0.9573071314750301 1103 64-65 0.8682994697526674 1103 66-67 0.747711908750798 1103 68-69 1.0524623500291987 1103 70-71 1.0561078575457827 1103 72-73 1.0319789163672972 1103 74-75 1.0890776658664159 1103 76-77 1.2062661531605343 1103 78-79 1.4248397397298191 1103 80-81 1.3711008815252193 1103 82-83 1.4242471262115437 1103 84-85 1.479695948878792 1103 86-87 1.8788397208179397 1103 88-89 1.5451824954008302 1103 90-91 1.6148722157624817 1103 92-93 1.7601667958054605 1103 94-95 1.5314961307861878 1103 96-97 1.7370553417962746 1103 98-99 1.9546848863836672 1103 100-101 1.7551309319650663 1104 1 -0.14434021268206365 1104 2 -0.03645924775327103 1104 3 0.016009930696853303 1104 4 -0.03359398948185799 1104 5 0.04281537883763775 1104 6 -0.1581575281366625 1104 7 0.09050767467816456 1104 8 -0.12424996549861334 1104 9 0.24954591408865667 1104 10-11 0.037388233672508875 1104 12-13 0.07692656942008824 1104 14-15 0.0919177888356657 1104 16-17 -0.09230993342946192 1104 18-19 0.06827122634362581 1104 20-21 -0.07115249763900522 1104 22-23 -0.07378202437160297 1104 24-25 0.01559469642435829 1104 26-27 0.010432212092609916 1104 28-29 -0.02762701774005194 1104 30-31 0.47832004921068716 1104 32-33 0.4691816135864002 1104 34-35 0.43030818966322926 1104 36-37 0.6396691043833584 1104 38-39 0.6499808155872913 1104 40-41 0.26101719989934935 1104 42-43 -0.0776100751688773 1104 44-45 -0.06928857560915702 1104 46-47 0.23637475363803873 1104 48-49 0.6214302284113984 1104 50-51 0.5478811711630733 1104 52-53 0.8780461032717959 1104 54-55 0.8195687260106155 1104 56-57 1.1490375479534158 1104 58-59 1.2779703301736127 1104 60-61 0.9679911696801256 1104 62-63 0.9941240517544827 1104 64-65 1.2424596764710145 1104 66-67 1.2567749769869927 1104 68-69 1.3602142880136974 1104 70-71 1.356873481050421 1104 72-73 1.1159103930636327 1104 74-75 1.38534525128129 1104 76-77 2.070578527550424 1104 78-79 1.9712078132296753 1104 80-81 1.8634159355973772 1104 82-83 1.2644615007928444 1104 84-85 1.32435093978701 1104 86-87 1.0298589571084946 1104 88-89 1.0666582347060292 1104 90-91 0.917752874198694 1104 92-93 0.9323172408241227 1104 94-95 0.7502060551808647 1104 96-97 1.5055882157555054 1104 98-99 1.709254701676997 1104 100-101 1.398915026136759 1105 1 -0.18029244398313082 1105 2 -0.060469304321465245 1105 3 -0.029244626185608524 1105 4 0.10279821667779032 1105 5 0.007868804356185422 1105 6 0.03882550140451002 1105 7 0.3221858644958928 1105 8 0.05337417026505875 1105 9 0.15551700145509528 1105 10-11 0.13870815824824234 1105 12-13 0.04681971838299859 1105 14-15 0.3846896304447185 1105 16-17 0.05985851408782139 1105 18-19 -0.05875580068340014 1105 20-21 -0.027217865332282543 1105 22-23 -0.22752181066952204 1105 24-25 -0.15517840225571433 1105 26-27 0.11935741636665398 1105 28-29 0.04069479244222407 1105 30-31 -0.17642539390684675 1105 32-33 -0.17538155423257962 1105 34-35 -0.1815082779671897 1105 36-37 -0.007219644830975369 1105 38-39 0.17908200980476607 1105 40-41 0.4923811219609391 1105 42-43 0.28499206185185955 1105 44-45 0.40651280716897276 1105 46-47 -0.22138765993832976 1105 48-49 -0.05870804940129659 1105 50-51 0.18735320133278677 1105 52-53 -0.13905006266157827 1105 54-55 -0.22530870956448723 1105 56-57 0.19309788736259392 1105 58-59 0.08048447222263988 1105 60-61 -0.09624515967248115 1105 62-63 -0.14421661449316048 1105 64-65 -0.0031720017188021643 1105 66-67 -0.09243935362268729 1105 68-69 -0.5628529652861154 1105 70-71 -1.1289216163725797 1105 72-73 -0.5937690538251168 1105 74-75 -0.4562009460788623 1105 76-77 -0.6740474938197814 1105 78-79 -0.8749266933699467 1105 80-81 -0.3066657740192156 1105 82-83 -0.21253409945856916 1105 84-85 -0.558364333625935 1105 86-87 0.0061003147452005635 1105 88-89 -0.38111046422546124 1105 90-91 -0.04170658526076565 1105 92-93 0.16764093661293344 1105 94-95 -0.025972448904614254 1105 96-97 -0.38850355042928086 1105 98-99 -0.15410167795845453 1105 100-101 -0.2566475131467101 1106 1 0.009120637737645154 1106 2 -0.08464969773262965 1106 3 -0.09900314209797045 1106 4 0.19849628160662292 1106 5 -0.1101225597890263 1106 6 0.054529963262815784 1106 7 -0.005432878507463101 1106 8 -0.14445087371383636 1106 9 -0.04240399646590731 1106 10-11 0.008242781629014928 1106 12-13 -0.29116205036799414 1106 14-15 -0.2843172462544672 1106 16-17 0.14121146467154233 1106 18-19 -0.053622264780635476 1106 20-21 -0.02758568877703027 1106 22-23 -0.025522770208944223 1106 24-25 0.09601302585878813 1106 26-27 0.2693973971758652 1106 28-29 0.6831064348538618 1106 30-31 0.2921496900528524 1106 32-33 0.2645992549789966 1106 34-35 0.1426241384729181 1106 36-37 -0.15362936816377726 1106 38-39 0.40375816525016006 1106 40-41 0.206166504977098 1106 42-43 0.1706629398304358 1106 44-45 0.44847666451744317 1106 46-47 0.1800516491932882 1106 48-49 0.30111923350290937 1106 50-51 0.19776420405147732 1106 52-53 0.22469533954535947 1106 54-55 0.6365495984476652 1106 56-57 0.9156950407289806 1106 58-59 1.1569437717588649 1106 60-61 1.413622104040936 1106 62-63 1.3496903101829965 1106 64-65 1.441606504598166 1106 66-67 1.3244645325119677 1106 68-69 1.572186215906914 1106 70-71 1.5548781597348906 1106 72-73 2.4840815778716667 1106 74-75 2.2041700758306213 1106 76-77 1.8041549689980663 1106 78-79 1.9300629116196575 1106 80-81 2.4063359851363906 1106 82-83 2.145677958753488 1106 84-85 2.053757847727013 1106 86-87 2.4018143719977196 1106 88-89 1.9614291167756583 1106 90-91 1.478107903784494 1106 92-93 1.0841130300081048 1106 94-95 0.868494207100504 1106 96-97 0.584341483954212 1106 98-99 0.9141535595275521 1106 100-101 1.635436286629396 1107 1 0.28818772449703545 1107 2 0.12026077640861388 1107 3 0.024949828008843156 1107 4 0.2932861071656845 1107 5 0.2500941465815245 1107 6 0.24226530484430953 1107 7 0.39386013617016147 1107 8 -0.10791049101960226 1107 9 0.30774585368394725 1107 10-11 0.11171623125631669 1107 12-13 0.4389924105556986 1107 14-15 0.21527931103440068 1107 16-17 0.5686686228979312 1107 18-19 0.492946551018953 1107 20-21 0.5014237633093472 1107 22-23 0.4939769608292508 1107 24-25 0.1976998006224875 1107 26-27 0.35842375543299454 1107 28-29 0.45083870258613246 1107 30-31 0.39210742462596926 1107 32-33 0.5766717978207723 1107 34-35 0.2568560603971477 1107 36-37 0.46821033059899975 1107 38-39 0.4930342737570328 1107 40-41 0.38037690995673046 1107 42-43 0.3363434132032168 1107 44-45 0.3756072262633907 1107 46-47 0.8427927542420832 1107 48-49 1.2363954957022472 1107 50-51 1.4536045175841004 1107 52-53 1.4072819836704689 1107 54-55 1.6080012237454469 1107 56-57 1.8431329873976985 1107 58-59 1.315496757234925 1107 60-61 2.1457169322896092 1107 62-63 2.1924629221863796 1107 64-65 2.060218661671861 1107 66-67 2.273899712716382 1107 68-69 1.613068410635261 1107 70-71 1.6898393523883897 1107 72-73 1.77386871381265 1107 74-75 1.6339710440931263 1107 76-77 1.5143870446147574 1107 78-79 1.5119809935377404 1107 80-81 1.3051102877568468 1107 82-83 1.219354282429812 1107 84-85 1.0653808401192393 1107 86-87 1.4789679876128723 1107 88-89 1.4517080685930708 1107 90-91 1.3541165105623314 1107 92-93 0.9642713332433317 1107 94-95 0.6589345360023735 1107 96-97 0.36828830636257237 1107 98-99 0.06488744103066324 1107 100-101 0.5017238452246495 1108 1 -0.003336540711437408 1108 2 0.1028308379260423 1108 3 -0.4404466176507569 1108 4 -0.11853890992249205 1108 5 -0.15045267501793091 1108 6 0.014394491447184521 1108 7 -0.2535253730574496 1108 8 0.08040119729208328 1108 9 -0.15525824747805217 1108 10-11 -0.026014459105958565 1108 12-13 0.09701530871017638 1108 14-15 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-2.5517938902212123 2316 62-63 -2.2095933548698987 2316 64-65 -2.61781164135844 2316 66-67 -2.407918469101805 2316 68-69 -2.352840680273829 2316 70-71 -1.7852688727198363 2316 72-73 -1.8403087753648357 2316 74-75 -1.7675441074220224 2316 76-77 -1.5299852497575372 2316 78-79 -1.7071748506181024 2316 80-81 -1.9665347338881745 2316 82-83 -2.1448232067476773 2316 84-85 -2.467086692348296 2316 86-87 -2.0524172937724074 2316 88-89 -2.7652832733982713 2316 90-91 -2.9072471258013053 2316 92-93 -2.7749061559341612 2316 94-95 -3.8232083211404877 2316 96-97 -3.103573165498897 2316 98-99 -3.583380957237736 2316 100-101 -3.7266311331303292 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 1.0 4 5.0 5 13.0 6 38.0 7 64.0 8 100.0 9 194.0 10 362.0 11 602.0 12 838.0 13 1168.0 14 1400.0 15 2102.0 16 3011.0 17 4150.0 18 3551.0 19 2050.0 20 1707.0 21 1731.0 22 1721.0 23 1896.0 24 2019.0 25 2228.0 26 2465.0 27 2819.0 28 3269.0 29 3868.0 30 4396.0 31 5156.0 32 6280.0 33 8030.0 34 11153.0 35 17438.0 36 17925.0 37 21194.0 38 13324.0 39 1041.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.35385110103236 19.4655286764164 17.08325238328119 14.097367839270042 2 15.311453449430676 26.02478231748158 39.62893503014065 19.034829202947087 3 20.523915096551214 33.09734157629991 32.97208956523486 13.406653761914011 4 10.782192872594786 20.02129782802339 38.19075620684343 31.00575309253839 5 9.956733152050152 42.29300898825231 31.672538277095363 16.077719582602175 6 22.405582977811413 40.18190220412428 18.41190535191647 19.00060946614784 7 21.491383756052805 42.03374165332762 20.403721092216916 16.071153498402662 8 25.65584585194661 30.7894260972065 20.494806142882975 23.059921907963915 9 28.74537040137699 18.64966412387567 21.934753635030237 30.670211839717098 10-11 23.1761223218651 29.320009912196692 24.93285826228476 22.571009503653446 12-13 30.287118832503968 29.077562939100265 26.20101666990376 14.434301558492008 14-15 25.555384399006105 27.96177106844104 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40-41 15.715409989785837 28.957318196279363 40.69087925520336 14.636392558731433 42-43 15.13419200139316 30.699392502394492 38.73416788902955 15.432247607182806 44-45 15.760814965138941 31.0956619582471 36.9332985057633 16.210224570850663 46-47 16.174456414360776 30.66214808836678 35.11899778647709 18.044397710795362 48-49 17.168480026254365 30.49826366214917 34.992314570168475 17.340941741427983 50-51 18.4139847962225 31.448377482334816 34.32570911891765 15.811928602525033 52-53 16.791743269807142 31.96100690851492 35.00437014637478 16.242879675303147 54-55 15.178741858245568 31.351070776752678 35.41684108299985 18.053346282001907 56-57 17.360190208291474 32.88895586363941 32.39736119482955 17.35349273323957 58-59 16.891923568926604 31.19562523859595 33.424530007836 18.487921184641454 60-61 16.741566261025646 30.56506218563937 34.3370548720456 18.35631668128939 62-63 19.580941792634164 30.072801066230888 33.94659469161682 16.39966244951812 64-65 18.618093649741127 30.92979953248806 34.583827301893486 15.86827951587732 66-67 17.01800622197516 30.953824103623678 34.771062792387625 17.257106882013534 68-69 16.896055187194428 32.53398968588842 34.119951778179626 16.45000334873753 70-71 17.389295031295106 32.2140323968802 32.464862077164454 17.93181049466024 72-73 16.627091113083374 31.392065910879648 32.34857745700555 19.63226551903143 74-75 16.932232224186894 32.02258479344157 32.82497454857204 18.220208433799495 76-77 17.72240920279299 30.383282262520723 33.64779558279332 18.246512951892967 78-79 16.440874944319596 30.2402379269808 34.47663767378148 18.84224945491813 80-81 16.50715620416446 32.426947780136764 32.77755825089913 18.288337764799646 82-83 16.18090788834748 31.71715142077326 32.30220525443311 19.79973543644614 84-85 16.292892998148155 29.937613228808424 32.06606367268209 21.703430100361327 86-87 16.926080462926375 30.08552618360335 32.486889780391266 20.50150357307901 88-89 17.954196121505188 28.564635440910052 32.87779493069811 20.603373506886655 90-91 16.04804736422635 28.863914915846788 34.126755563890136 20.961282156036727 92-93 15.466710423210614 30.642082632893757 33.19011995097481 20.701086992920818 94-95 15.327274310322888 31.203745253132766 32.183592415728455 21.285388020815894 96-97 15.026689259331194 30.284104988915754 31.394873786927956 23.294331964825098 98-99 15.98341716283462 30.084521569073946 31.236144691281954 22.695916576809477 100-101 16.77354756395273 29.793147790328145 31.371078196631828 22.062226449087298 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2603.0 1 1781.5 2 1021.5 3 1033.5 4 823.5 5 799.0 6 1385.5 7 2697.5 8 3829.0 9 3845.5 10 2856.0 11 1914.0 12 1668.5 13 1575.5 14 1413.5 15 1230.5 16 1086.0 17 984.5 18 937.5 19 870.0 20 827.5 21 836.5 22 873.0 23 953.0 24 1012.0 25 1092.0 26 1225.5 27 1294.5 28 1320.0 29 1481.5 30 1743.5 31 1883.0 32 2151.0 33 2535.0 34 2848.0 35 3055.0 36 3233.0 37 3445.0 38 3452.0 39 3235.0 40 2988.0 41 2918.5 42 2830.5 43 2849.5 44 3300.5 45 3515.5 46 3645.5 47 4846.5 48 11718.0 49 11329.5 50 4267.5 51 2871.5 52 2004.0 53 1665.5 54 1540.5 55 1546.0 56 1566.0 57 1521.0 58 1563.5 59 1696.0 60 1712.0 61 1611.5 62 1356.0 63 864.5 64 455.0 65 332.0 66 271.0 67 224.5 68 184.0 69 127.5 70 88.5 71 74.5 72 66.0 73 54.0 74 46.0 75 36.5 76 23.0 77 12.5 78 12.0 79 9.0 80 4.5 81 4.5 82 3.5 83 2.0 84 1.5 85 0.5 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.028129206823341885 2 0.00736717321563716 3 0.008036916235240539 4 0.0 5 0.003348715098016891 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.006362558686232092 20-21 0.0010046145294050673 22-23 0.0 24-25 0.006362558686232092 26-27 0.00368358660781858 28-29 0.005357944156827025 30-31 0.02879894984294526 32-33 6.697430196033782E-4 34-35 0.002344100568611824 36-37 0.016408703980282765 38-39 0.007032301705835471 40-41 0.005692815666628714 42-43 0.006697430196033782 44-45 0.0026789720784135126 46-47 0.0010046145294050673 48-49 0.0016743575490084455 50-51 0.004018458117620269 52-53 0.0016743575490084455 54-55 0.002344100568611824 56-57 6.697430196033782E-4 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.006697430196033782 66-67 0.0010046145294050673 68-69 6.697430196033782E-4 70-71 0.004353329627421958 72-73 0.012390245862662496 74-75 0.004688201137223648 76-77 0.005692815666628714 78-79 0.01439947492147263 80-81 0.0 82-83 0.005692815666628714 84-85 3.348715098016891E-4 86-87 0.0 88-89 3.348715098016891E-4 90-91 0.0 92-93 0.0 94-95 0.0013394860392067563 96-97 0.0 98-99 0.0 100-101 0.0010046145294050673 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 149311.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.258848979646515 #Duplication Level Percentage of deduplicated Percentage of total 1 78.7594961366145 32.49526156813631 2 11.172975780793456 9.219682407860104 3 3.770859035127589 4.667439103615942 4 1.8797480683072527 3.1022496668028476 5 1.014544510096747 2.092946936260557 6 0.6671644698396209 1.6515862863419308 7 0.475618466333355 1.3736429332065287 8 0.33926368417635216 1.1198103287768484 9 0.24511395363937408 0.9101807636409909 >10 1.467437179403935 11.03133727588724 >50 0.09739627296928771 2.757332011707108 >100 0.07467047594312058 5.893068829490124 >500 0.02110252581001234 6.250711601958328 >1k 0.012986169729238361 13.904534829985735 >5k 0.0016232712161547952 3.530215456329407 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5271 3.530215456329407 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4133 2.768047900020762 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3208 2.148535606887637 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3150 2.1096905117506415 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2975 1.99248548332005 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2662 1.7828559181841928 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2190 1.4667372129313982 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1362 0.912189992699801 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1081 0.7239922041912518 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 891 0.59674103046661 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 834 0.5585656783492174 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 833 0.557895935329614 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 819 0.5485195330551668 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 805 0.5391431307807194 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 759 0.508334951878964 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 720 0.48221497411443226 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 714 0.478196515996812 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 639 0.42796578952655867 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 629 0.42126835933052487 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 595 0.39849709666401006 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 575 0.38510223627194246 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 520 0.34826637019375667 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 469 0.3141094761939844 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 467 0.3127699901547776 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 397 0.26588797878254117 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 361 0.24177723007681953 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 338 0.22637314062594183 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 298 0.1995834198418067 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 265 0.17748190019489524 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 247 0.1654265258420344 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 237 0.15872909564600063 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 235 0.15738960960679388 No Hit GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG 230 0.15404089450877698 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 229 0.1533711514891736 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 223 0.14935269337155332 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 217 0.14533423525393308 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 206 0.13796706203829592 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 204 0.13662757599908915 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 203 0.13595783297948577 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 200 0.13394860392067562 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 197 0.1319393748618655 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 187 0.12524194466583172 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 185 0.12390245862662495 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 181 0.12122348654821145 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 175 0.11720502843059118 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 3.348715098016891E-4 0.0 18-19 0.0 0.0 0.0 6.697430196033782E-4 0.0 20-21 0.0 0.0 0.0 0.0010046145294050671 0.0 22-23 0.0 0.0 0.0 0.003348715098016891 0.0 24-25 0.0 0.0 0.0 0.008371787745042228 0.0 26-27 0.0 0.0 0.0 0.021096905117506413 0.0 28-29 0.0 0.0 0.0 0.03717073758798749 0.0 30-31 0.0 0.0 0.0 0.06697430196033782 0.0 32-33 0.0 0.0 0.0 0.08338300594062059 0.0 34-35 0.0 0.0 0.0 0.10213581048951517 0.0 36-37 0.0 0.0 0.0 0.12658143070503847 0.0 38-39 0.0 0.0 0.0 0.15471063752838038 0.0 40-41 0.0 0.0 0.0 0.19422547568497966 0.0 42-43 0.0 0.0 0.0 0.24780491725324993 0.0 44-45 0.0 0.0 0.0 0.28397104031183235 0.0 46-47 0.0 0.0 0.0 0.36266584511522926 0.0 48-49 0.0 0.0 0.0 0.47116421429097655 0.0 50-51 0.0 0.0 0.0 0.5157021250946012 0.0 52-53 0.0 0.0 0.0 0.585020527623551 0.0 54-55 0.0 0.0 0.0 0.6975373549169184 0.0 56-57 0.0 0.0 0.0 0.8699961824647882 0.0 58-59 0.0 0.0 0.0 1.0508267977577004 0.0 60-61 0.0 0.0 0.0 1.301980430108967 0.0 62-63 0.0 0.0 0.0 1.524000241107487 0.0 64-65 0.0 0.0 0.0 1.7165513592434583 0.0 66-67 0.0 0.0 0.0 1.994829583888662 0.0 68-69 0.0 0.0 0.0 2.3045857304552246 0.0 70-71 0.0 0.0 0.0 2.471686613846267 0.0 72-73 0.0 0.0 0.0 2.714468458452492 0.0 74-75 0.0 0.0 0.0 2.9693056774115774 0.0 76-77 0.0 0.0 0.0 3.1792701140572364 0.0 78-79 0.0 0.0 0.0 3.421717087153659 0.0 80-81 0.0 0.0 0.0 3.8436551895037874 0.0 82-83 0.0 0.0 0.0 4.314484532284962 0.0 84-85 0.0 0.0 0.0 5.076652088593606 0.0 86-87 0.0 0.0 0.0 6.148575791468813 0.0 88-89 0.0 0.0 0.0 7.010869929208162 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGTAAG 25 0.0050119716 57.002216 1 GGGATTC 25 0.0050186235 56.983124 8 TAAGGTG 45 9.612143E-4 42.209724 5 TCGGGAG 95 8.6527143E-7 34.989635 3 GTCGGGA 100 1.2887867E-6 33.251293 2 TAGCATC 60 0.0039457614 31.65729 5 TGGGACC 95 3.477774E-5 29.991116 6 CGGGAGT 115 3.843883E-6 28.904482 4 GTGGGTA 120 5.3529657E-6 27.700129 9 ACCACTC 35 0.00838021 27.14391 94-95 CTATTAA 70 0.008367021 27.14391 1 TTAATAG 70 0.008380711 27.134819 4 GGGAATG 70 0.008380711 27.134819 7 CTAGCAT 70 0.008380711 27.134819 4 TATGAGA 45 8.9474366E-4 26.381077 10-11 GAGTACT 2650 0.0 25.63307 12-13 ATGGGAC 225 1.0004442E-10 25.32583 5 CATGGGG 410 0.0 24.322063 4 GTACTTT 2835 0.0 23.968393 14-15 GGTCGGG 140 1.7655295E-5 23.750921 1 >>END_MODULE