##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936957_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 161604 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.93905472636816 33.0 31.0 34.0 30.0 34.0 2 31.966714932798695 33.0 31.0 34.0 30.0 34.0 3 31.8730353209079 33.0 31.0 34.0 30.0 34.0 4 35.53710922996955 37.0 35.0 37.0 33.0 37.0 5 35.542474196183264 37.0 35.0 37.0 33.0 37.0 6 35.73594094205589 37.0 35.0 37.0 35.0 37.0 7 35.727005519665354 37.0 35.0 37.0 35.0 37.0 8 35.69052127422589 37.0 35.0 37.0 33.0 37.0 9 37.24479592089305 39.0 37.0 39.0 34.0 39.0 10-11 37.142734709536896 39.0 37.0 39.0 34.0 39.0 12-13 37.036744139996536 39.0 37.0 39.0 33.0 39.0 14-15 37.88869396797109 40.0 37.5 41.0 33.0 41.0 16-17 37.90640083166258 40.0 37.5 41.0 33.0 41.0 18-19 38.07300561867281 40.0 37.5 41.0 33.5 41.0 20-21 38.101095888715626 40.0 38.0 41.0 34.0 41.0 22-23 37.971151704165734 40.0 37.5 41.0 33.5 41.0 24-25 37.9316353555605 40.0 37.0 41.0 33.0 41.0 26-27 37.737735452092764 40.0 36.5 41.0 33.0 41.0 28-29 37.54869000767307 39.5 36.0 41.0 33.0 41.0 30-31 37.11941845498873 39.0 35.5 41.0 33.0 41.0 32-33 36.59272357119873 38.0 35.0 41.0 32.5 41.0 34-35 36.25566198856464 38.0 35.0 40.0 31.5 41.0 36-37 35.906292542263806 38.0 35.0 40.0 31.0 41.0 38-39 35.54671604663251 37.5 35.0 40.0 30.5 41.0 40-41 35.13619402985074 37.0 35.0 40.0 30.0 41.0 42-43 34.710892057127296 37.0 35.0 40.0 27.5 41.0 44-45 34.118162297962925 37.0 35.0 40.0 23.5 41.0 46-47 33.31431771490804 36.0 33.5 40.0 17.5 41.0 48-49 32.62482364297914 36.0 33.0 40.0 4.5 41.0 50-51 31.659389619068833 35.5 32.0 39.0 2.0 40.5 52-53 31.74833543724165 35.0 32.5 39.0 2.0 40.0 54-55 32.054249894804585 35.0 33.0 40.0 2.0 41.0 56-57 31.923983936041186 35.0 33.0 40.0 2.0 41.0 58-59 31.680373010569042 35.0 33.0 40.0 2.0 41.0 60-61 31.34837008984926 35.0 32.5 39.5 2.0 41.0 62-63 30.936087596841663 35.0 31.5 39.0 2.0 41.0 64-65 30.462609217593624 35.0 31.0 39.0 2.0 41.0 66-67 30.029278359446547 35.0 30.5 38.0 2.0 40.0 68-69 29.627206009752236 35.0 30.0 37.5 2.0 40.0 70-71 29.130429939852974 35.0 29.0 37.0 2.0 39.5 72-73 28.65457228781466 35.0 28.5 36.5 2.0 39.0 74-75 28.18799349026014 35.0 27.5 36.0 2.0 39.0 76-77 27.684457067894357 34.5 26.5 35.0 2.0 37.5 78-79 27.25861983614267 34.0 25.5 35.0 2.0 37.0 80-81 26.814033068488403 34.0 24.5 35.0 2.0 36.5 82-83 26.39136098116383 34.0 23.0 35.0 2.0 36.0 84-85 25.968936412465037 34.0 20.0 35.0 2.0 36.0 86-87 25.530466448850277 33.0 17.5 35.0 2.0 35.0 88-89 25.034139006460236 33.0 8.5 35.0 2.0 35.0 90-91 24.51731083389025 33.0 2.0 35.0 2.0 35.0 92-93 24.192352912056634 33.0 2.0 35.0 2.0 35.0 94-95 23.82470730922502 33.0 2.0 35.0 2.0 35.0 96-97 23.234381574713495 32.0 2.0 35.0 2.0 35.0 98-99 22.606408257221354 32.0 2.0 35.0 2.0 35.0 100-101 20.647103413281847 28.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.22396147705570968 1101 2 0.2833209490887434 1101 3 0.29226475507576666 1101 4 0.09172279097668934 1101 5 0.06613315423845734 1101 6 -0.02358397102310761 1101 7 -0.22535823697545254 1101 8 0.060241373382304175 1101 9 0.09629128537526555 1101 10-11 0.1899817297466413 1101 12-13 0.37328244701677704 1101 14-15 0.5598773406143351 1101 16-17 0.49843536904761976 1101 18-19 0.2900031752047312 1101 20-21 0.3534378747990061 1101 22-23 0.4171998019680885 1101 24-25 0.5875090873888738 1101 26-27 0.5916284274795132 1101 28-29 0.5608149249357268 1101 30-31 0.8546595228428444 1101 32-33 0.9542416065736248 1101 34-35 0.9756310279326854 1101 36-37 0.9283859233188352 1101 38-39 0.7448574958462331 1101 40-41 0.9209484715622125 1101 42-43 0.8255478287868598 1101 44-45 1.5253896984726083 1101 46-47 1.7371579440024618 1101 48-49 1.5704412724444339 1101 50-51 1.5483550241018094 1101 52-53 1.7827341011743663 1101 54-55 2.193310892850576 1101 56-57 2.130386674499139 1101 58-59 2.19842453881299 1101 60-61 2.37366047456797 1101 62-63 2.4800770194327697 1101 64-65 2.4123853922056817 1101 66-67 2.2602315037595595 1101 68-69 2.315412335355255 1101 70-71 1.9915292393313635 1101 72-73 1.9249481317302006 1101 74-75 1.7795272914126663 1101 76-77 2.098596561390785 1101 78-79 2.048252281736705 1101 80-81 2.2194625838548916 1101 82-83 2.424545004564795 1101 84-85 2.3252439370845757 1101 86-87 2.870395403476703 1101 88-89 2.901695385528985 1101 90-91 3.226822129712282 1101 92-93 2.9780472975588523 1101 94-95 3.0351318283226227 1101 96-97 3.1887633712123105 1101 98-99 3.0920246385458086 1101 100-101 2.930968611672764 1102 1 0.27391922948494596 1102 2 0.5621247392993745 1102 3 0.4872222057366393 1102 4 0.3127228513303635 1102 5 0.42585613088158425 1102 6 0.2214066741576488 1102 7 0.308952825852721 1102 8 0.25282994239672973 1102 9 0.15917981214212062 1102 10-11 0.4657452037040315 1102 12-13 0.3724374956014813 1102 14-15 0.29745957249313904 1102 16-17 0.7604834709246191 1102 18-19 0.8967839103124575 1102 20-21 1.0689382067441997 1102 22-23 0.9573425384036014 1102 24-25 0.22522107966360494 1102 26-27 0.6603561720627695 1102 28-29 0.7920449327817067 1102 30-31 0.7084678395789226 1102 32-33 1.0750168494971533 1102 34-35 0.7987344137737082 1102 36-37 0.511998392387575 1102 38-39 0.6898979328068222 1102 40-41 0.9873903207987382 1102 42-43 1.3404627301086052 1102 44-45 0.9901431537203678 1102 46-47 0.8579633637622521 1102 48-49 0.7295486416278507 1102 50-51 0.785831637053704 1102 52-53 0.7487776161722515 1102 54-55 1.1244926177585413 1102 56-57 0.700593084059161 1102 58-59 0.7268435743613075 1102 60-61 0.7385210575844425 1102 62-63 0.7090513087682808 1102 64-65 1.0098188522817289 1102 66-67 0.7375309786826101 1102 68-69 1.0409162885208048 1102 70-71 1.1589427826954761 1102 72-73 1.5019895947032218 1102 74-75 1.7022745905858194 1102 76-77 2.318178310440217 1102 78-79 1.9827836354695805 1102 80-81 2.2475270415771433 1102 82-83 2.179433652038991 1102 84-85 2.1523834747754442 1102 86-87 2.4276257734447135 1102 88-89 2.694712465618313 1102 90-91 2.493434478073681 1102 92-93 2.789208200449796 1102 94-95 2.692134363176873 1102 96-97 2.156051680705943 1102 98-99 2.432781473599224 1102 100-101 2.2218582248057253 1103 1 0.24544988877494234 1103 2 0.2979147676205294 1103 3 0.1315118718105346 1103 4 0.1555569420897598 1103 5 0.24580278174109793 1103 6 -0.09215125247081346 1103 7 -0.09136331720201696 1103 8 0.04391207372782446 1103 9 -0.08754349682185136 1103 10-11 0.03224227279446268 1103 12-13 0.062386780986450674 1103 14-15 0.20403271350611618 1103 16-17 0.6460380385331277 1103 18-19 0.3182915175047185 1103 20-21 0.37672487604539384 1103 22-23 0.397415317169326 1103 24-25 0.36098146957338173 1103 26-27 0.6399221339938705 1103 28-29 0.46554489985013703 1103 30-31 0.4949889487141448 1103 32-33 0.5097217826460749 1103 34-35 0.15340740330147895 1103 36-37 0.281666771495793 1103 38-39 -0.040203167107556226 1103 40-41 0.3047106790941996 1103 42-43 -0.03745171367720701 1103 44-45 0.1259529409824296 1103 46-47 0.08315766001463487 1103 48-49 -0.3623798310060167 1103 50-51 -0.023478117276798827 1103 52-53 0.23454294581724966 1103 54-55 0.30250420967054836 1103 56-57 0.28550713266615446 1103 58-59 0.2765290778849838 1103 60-61 0.1802568805443343 1103 62-63 0.08755852078171955 1103 64-65 -0.118293180913291 1103 66-67 0.183712563349669 1103 68-69 0.47580234529482723 1103 70-71 0.3437135789807968 1103 72-73 0.5424954235907897 1103 74-75 0.4685921826295534 1103 76-77 0.593074576000383 1103 78-79 0.9009651542735035 1103 80-81 1.2366596240707253 1103 82-83 1.2169262424361484 1103 84-85 0.5654029702638184 1103 86-87 0.699624061003167 1103 88-89 0.9646854617323832 1103 90-91 0.6287832233809283 1103 92-93 0.585205368334929 1103 94-95 0.9174109883497614 1103 96-97 0.3402708732238935 1103 98-99 0.2278812562508712 1103 100-101 0.2695925329134745 1104 1 0.05280811837383581 1104 2 -0.04200224482760717 1104 3 -0.06373017521574553 1104 4 -0.11219778359027544 1104 5 0.2482370832625378 1104 6 0.07743842018939517 1104 7 -0.0053328884329957305 1104 8 0.16457597414276393 1104 9 0.013148066110375112 1104 10-11 -0.0963976534388209 1104 12-13 0.07021527337926159 1104 14-15 0.38920560423917294 1104 16-17 -0.18626256082141168 1104 18-19 0.5512283547569012 1104 20-21 0.5518747146807073 1104 22-23 0.23615206221312945 1104 24-25 0.014268698711227046 1104 26-27 0.5484514101580018 1104 28-29 0.4083941391309125 1104 30-31 0.4864043475154318 1104 32-33 0.3522390717193744 1104 34-35 0.23746457250386754 1104 36-37 0.13350632889551406 1104 38-39 0.2625169804258647 1104 40-41 0.03397762238603974 1104 42-43 0.17133574598162227 1104 44-45 -0.42795208437487986 1104 46-47 -0.26068742988854154 1104 48-49 -0.41759421551500253 1104 50-51 -0.31242233120026697 1104 52-53 -0.4017779393833045 1104 54-55 -0.34042801716209325 1104 56-57 -0.2402005667344902 1104 58-59 -0.1110135684958351 1104 60-61 -0.1631456090822212 1104 62-63 0.16579734051431316 1104 64-65 0.0081521856150637 1104 66-67 0.18634050249213274 1104 68-69 0.7141702567747714 1104 70-71 0.4521967509494438 1104 72-73 0.5248467375603632 1104 74-75 0.7949730032842375 1104 76-77 0.39040053266244 1104 78-79 0.7248471275330708 1104 80-81 0.7478445018946047 1104 82-83 0.535782858388199 1104 84-85 0.4142088716008381 1104 86-87 0.7966733924923339 1104 88-89 0.9832838941897393 1104 90-91 0.773117017756217 1104 92-93 0.6857955020370952 1104 94-95 0.8260232520657596 1104 96-97 -0.11982133516707094 1104 98-99 0.3524640132817609 1104 100-101 0.6928106057581083 1105 1 0.23481459086574574 1105 2 0.09256086196851143 1105 3 -0.0773871331445477 1105 4 0.14355194672256033 1105 5 0.19458012533373648 1105 6 0.03998426700708535 1105 7 0.13512018600066256 1105 8 -0.14746286080869453 1105 9 -0.05448947434269513 1105 10-11 -0.01828546357906191 1105 12-13 -0.011597024355360475 1105 14-15 0.012053500679300555 1105 16-17 -0.12091196750964883 1105 18-19 0.014097826170065275 1105 20-21 0.3084011440226746 1105 22-23 0.38635702445692033 1105 24-25 -0.06889203483785167 1105 26-27 -0.013091200413732906 1105 28-29 0.15211582197880347 1105 30-31 0.37147446617778 1105 32-33 0.5079995895187253 1105 34-35 0.567227247800524 1105 36-37 0.4440564907078155 1105 38-39 0.594178253349277 1105 40-41 0.18986759002357445 1105 42-43 0.30469065428798103 1105 44-45 0.17707488434141538 1105 46-47 0.04287244066162543 1105 48-49 0.3715967229963226 1105 50-51 0.6101882405365195 1105 52-53 0.35058452016362196 1105 54-55 0.5834231155239848 1105 56-57 0.5608566069440393 1105 58-59 0.8565053096055699 1105 60-61 0.8060776918886532 1105 62-63 1.0246430794571388 1105 64-65 1.2100615707283318 1105 66-67 0.8636867808092852 1105 68-69 1.314590968748881 1105 70-71 1.634613147928949 1105 72-73 1.6068208475927257 1105 74-75 1.4601725717847742 1105 76-77 1.5681783104402172 1105 78-79 2.296314225699202 1105 80-81 2.235622279672384 1105 82-83 2.362859449542462 1105 84-85 1.7222131865954466 1105 86-87 1.2144188078105635 1105 88-89 1.2756571412231814 1105 90-91 1.9009703079396054 1105 92-93 2.0332172927598577 1105 94-95 2.3308884084843164 1105 96-97 2.609164641100456 1105 98-99 2.13643380831952 1105 100-101 2.1281611990698615 1106 1 0.035996524170936794 1106 2 0.2845194943028311 1106 3 0.25482054942193955 1106 4 0.38553168500875756 1106 5 0.06483128616108758 1106 6 0.2729939757449529 1106 7 0.25589899562497465 1106 8 0.35522814805580794 1106 9 0.5725683559654513 1106 10-11 0.3942303658848445 1106 12-13 0.2118094762778142 1106 14-15 0.3147128506159902 1106 16-17 -0.12663695695668054 1106 18-19 -0.006295799832464866 1106 20-21 0.01014766154060709 1106 22-23 -0.07802668174822003 1106 24-25 -0.19951149452548833 1106 26-27 -0.28029255119465546 1106 28-29 -0.0432725275357555 1106 30-31 -0.21173574910292814 1106 32-33 -0.4156232471212036 1106 34-35 0.10722302661014993 1106 36-37 -0.08047917835086338 1106 38-39 -0.4222052417963553 1106 40-41 -0.6204426674690282 1106 42-43 -0.5373840607816618 1106 44-45 -0.11329024862608605 1106 46-47 -0.32608598061317906 1106 48-49 -0.3250579836309484 1106 50-51 -0.5824827176736953 1106 52-53 -0.19627069300649325 1106 54-55 -0.4035439591910759 1106 56-57 -0.39373921407748824 1106 58-59 -0.39729376173255204 1106 60-61 -0.6835803916909171 1106 62-63 -0.10370749040356131 1106 64-65 -0.1831159303269665 1106 66-67 -0.37634065692815355 1106 68-69 -0.19553988815276568 1106 70-71 -0.14242885291529106 1106 72-73 0.06785398393717301 1106 74-75 0.09625319652095143 1106 76-77 0.3480696147880451 1106 78-79 0.5107770792238924 1106 80-81 0.5644266274984702 1106 82-83 1.0156137762625939 1106 84-85 0.684776504451083 1106 86-87 0.4666613151976442 1106 88-89 0.4273297879095459 1106 90-91 0.19555663128278766 1106 92-93 0.3350587870612536 1106 94-95 -0.01891636146081055 1106 96-97 -0.37854114193035926 1106 98-99 -0.435748547104712 1106 100-101 0.027327513970664796 1107 1 0.11395754366119348 1107 2 0.10098626091952312 1107 3 -0.17246580739965367 1107 4 0.15002443863194515 1107 5 -0.003946824783440661 1107 6 0.06035029758402999 1107 7 -0.054758175789316965 1107 8 -0.06025161206413543 1107 9 0.3154469166850902 1107 10-11 -0.1303823277683236 1107 12-13 0.15596239981604043 1107 14-15 0.24587227090583497 1107 16-17 0.17749222845061752 1107 18-19 0.38816322065729025 1107 20-21 0.1769705001213211 1107 22-23 0.01036662160010593 1107 24-25 0.3088280856844108 1107 26-27 0.1302521764415303 1107 28-29 0.5846010356826312 1107 30-31 0.2805805927261602 1107 32-33 0.24120458896075547 1107 34-35 0.6969281740364366 1107 36-37 0.6223569036081571 1107 38-39 0.4765016547553671 1107 40-41 0.3421385419262677 1107 42-43 0.3989602670544201 1107 44-45 0.3487912106442792 1107 46-47 0.3933355586172027 1107 48-49 0.8953942902321259 1107 50-51 0.7122136841254054 1107 52-53 0.5969576928006042 1107 54-55 0.5269283046769857 1107 56-57 1.0047802761773887 1107 58-59 1.0442354736497528 1107 60-61 0.9296130116074366 1107 62-63 0.8102417849587589 1107 64-65 0.7497613810173611 1107 66-67 0.8202485484691415 1107 68-69 1.0996874981540827 1107 70-71 0.8048787432866078 1107 72-73 0.8287547835373736 1107 74-75 1.4320228117133524 1107 76-77 1.4101323334287237 1107 78-79 1.718142146690159 1107 80-81 1.9655073371436487 1107 82-83 1.803407379460996 1107 84-85 1.9893429712176989 1107 86-87 1.8474396760172347 1107 88-89 1.990142131737631 1107 90-91 1.78694843538074 1107 92-93 1.4677112108493553 1107 94-95 1.2855251677745727 1107 96-97 0.9888376686643454 1107 98-99 -0.06680801736958131 1107 100-101 1.0788642456048514 1108 1 -0.1216746402468516 1108 2 -0.0139562678161127 1108 3 0.2240859167382787 1108 4 0.2649669673675774 1108 5 0.007547428090120434 1108 6 0.1580514470093206 1108 7 -0.16970070452494923 1108 8 0.08342654885540668 1108 9 0.3326882959954318 1108 10-11 -0.0068191093775240574 1108 12-13 0.2852727446436276 1108 14-15 0.6769067536644542 1108 16-17 0.4476071709793601 1108 18-19 0.6123011516917671 1108 20-21 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-0.10139876728804964 2316 86-87 -0.7041946630632232 2316 88-89 -0.7929487446991494 2316 90-91 -0.54544201002156 2316 92-93 -1.227780886851793 2316 94-95 -0.9458864701564629 2316 96-97 -0.9709324462781836 2316 98-99 -0.9615637644960167 2316 100-101 -0.4787866164641166 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 395.0 3 117.0 4 110.0 5 101.0 6 125.0 7 130.0 8 197.0 9 244.0 10 352.0 11 559.0 12 944.0 13 1356.0 14 1556.0 15 2178.0 16 2993.0 17 4101.0 18 3448.0 19 1862.0 20 1762.0 21 1758.0 22 1880.0 23 2025.0 24 2193.0 25 2191.0 26 2539.0 27 2680.0 28 3044.0 29 3481.0 30 4030.0 31 4993.0 32 6088.0 33 7972.0 34 11086.0 35 19253.0 36 20734.0 37 22453.0 38 17531.0 39 3143.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.18054094200656 19.133502506653464 16.019062944853623 15.666893606486354 2 14.701841675330463 24.322986286449826 40.5224268528145 20.45274518540522 3 19.444891391794048 33.571384367844544 32.1337954081317 14.849928832229716 4 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16.208957809928094 32.03673221866066 33.23535599806933 18.518953973341915 50-51 17.57041904903344 32.74733298680726 32.8166382020247 16.8656097621346 52-53 16.033328382960818 33.30394049652236 33.12758347565654 17.535147644860274 54-55 14.896612324838104 32.59519617822965 33.974832998660276 18.533358498271973 56-57 16.35953540466452 34.245960780431055 30.888659245187277 18.505844569717148 58-59 15.840312864510981 32.617486626217875 32.082535341128136 19.45966516814302 60-61 16.516449617727776 31.546199090962535 33.06971865630772 18.867632635001964 62-63 18.90081465827978 31.37099593753771 32.432557866108915 17.295631538073593 64-65 18.192844147968465 31.758579720548013 33.586836796574296 16.46173933490922 66-67 16.637992920266356 32.03981731316682 33.65782112533109 17.664368641235736 68-69 16.35341370432716 33.44916575898604 33.07134369739578 17.12607683929102 70-71 16.619783688437078 32.96724380695972 31.55425582942642 18.85871667517678 72-73 16.182245202974297 32.37996218696719 31.192967147220184 20.244825462838328 74-75 16.244576889037425 32.96385005291595 32.22828744190077 18.563285616145862 76-77 17.04897130548885 31.358161471614963 32.76751008888119 18.825357134015004 78-79 15.75826780669099 31.175209112767714 33.9039644005434 19.162558679997897 80-81 15.513276445335833 33.21448136902607 32.48310847408315 18.789133711554946 82-83 15.425538499628622 32.360732854667 31.649851448378314 20.56387719732607 84-85 15.715125634360689 30.64271568263399 31.512253991830676 22.12990469117465 86-87 16.583068511292716 30.858085297967342 32.14439325067379 20.41445294006616 88-89 17.34374661563168 29.201335491077646 32.394941471701266 21.059976421589408 90-91 15.564144538345614 29.56525237271509 33.746862941083634 21.12374014785566 92-93 14.981632722534963 31.359611795908137 32.74853077253976 20.910224709017143 94-95 14.871667930132896 31.413586645419805 32.11968377245231 21.59506165199499 96-97 14.582959419231459 30.64383366650369 31.10150452719722 23.671702387067626 98-99 15.660704338676734 30.296775391471186 31.219904685275736 22.822615584576344 100-101 17.046372496525855 29.974218587995704 31.36449593161272 21.614912983865725 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1813.0 1 1262.0 2 792.0 3 855.0 4 754.0 5 824.0 6 1437.5 7 2770.0 8 4121.0 9 4290.0 10 3178.0 11 2224.5 12 2082.5 13 2010.0 14 1745.5 15 1459.0 16 1246.5 17 1114.5 18 1018.5 19 960.5 20 985.0 21 1005.0 22 1001.0 23 1063.0 24 1119.5 25 1210.0 26 1311.0 27 1345.5 28 1462.5 29 1662.5 30 1886.5 31 2107.5 32 2416.0 33 2739.5 34 3126.0 35 3391.0 36 3647.0 37 3794.5 38 3728.0 39 3612.5 40 3331.0 41 3217.0 42 3094.5 43 3103.5 44 3417.0 45 3680.5 46 3933.0 47 5124.0 48 12315.5 49 12069.0 50 4733.5 51 3161.0 52 2295.5 53 2017.5 54 1879.5 55 1779.0 56 1713.0 57 1621.5 58 1629.0 59 1672.5 60 1738.5 61 1733.5 62 1485.0 63 951.5 64 516.0 65 404.5 66 323.0 67 225.0 68 160.5 69 134.0 70 113.5 71 81.0 72 64.0 73 63.0 74 53.5 75 37.5 76 24.0 77 14.0 78 10.0 79 8.0 80 6.0 81 2.5 82 0.5 83 1.5 84 1.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021039083191010124 2 0.007425558773297691 3 0.008663151902180638 4 0.01794510036880275 5 0.05012252171975941 6 0.044553352639786145 7 0.02042028662656865 8 0.04702853889755204 9 0.022276676319893073 10-11 0.02134848147323086 12-13 0.033415014479839605 14-15 0.019182693497685702 16-17 0.014232320982153908 18-19 0.012994727853270958 20-21 0.0108289398777258 22-23 0.006497363926635479 24-25 9.281948466622114E-4 26-27 0.0 28-29 0.0021657879755451596 30-31 0.0 32-33 9.281948466622114E-4 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0012375931288829485 50-51 0.0 52-53 0.0 54-55 0.0021657879755451596 56-57 6.187965644414743E-4 58-59 3.0939828222073713E-4 60-61 0.002784584539986634 62-63 0.0015469914111036855 64-65 0.0012375931288829485 66-67 0.009900745031063588 68-69 0.012375931288829484 70-71 0.019492091779906438 72-73 0.012066533006608747 74-75 0.016707507239919803 76-77 0.02475186257765897 78-79 0.017635702086582015 80-81 0.036199599019826244 82-83 0.027227048835424865 84-85 0.014851117546595382 86-87 0.0108289398777258 88-89 0.009591346748842851 90-91 0.01701690552214054 92-93 0.02444246429543823 94-95 0.007116160491076954 96-97 0.015469914111036856 98-99 0.021039083191010124 100-101 0.03310561619761887 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 161604.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.86710724982055 #Duplication Level Percentage of deduplicated Percentage of total 1 79.18635651916323 34.7367639414866 2 11.070516708750052 9.71263087547338 3 3.6746554569691496 4.835895151110121 4 1.8648347463006587 3.272196232766516 5 0.997305722870322 2.187445855300611 6 0.6390091831121018 1.681889062151927 7 0.45986091323299155 1.4120937600554442 8 0.32020989970518116 1.1237345610257172 9 0.2595533988799707 1.0247271107150813 >10 1.3386748670494137 10.70765575109527 >50 0.08745821049216403 2.6967154278359446 >100 0.07335204750955691 6.594514987252791 >500 0.014106162982607102 4.075394173411549 >1k 0.012695546684346392 12.561570258161927 >5k 0.0014106162982607101 3.3767728521571247 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5457 3.3767728521571247 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3909 2.4188757704017227 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3342 2.068018118363407 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2894 1.7907972574936264 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2341 1.448602757357491 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1949 1.2060345040964333 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1876 1.1608623548922055 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1817 1.1243533575901588 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1093 0.6763446449345313 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1079 0.6676814930323507 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 940 0.5816687705749858 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 818 0.5061755897131259 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 796 0.49256206529541346 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 747 0.46224103363778124 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 601 0.37189673522932604 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 580 0.35890200737605504 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 557 0.34466968639390116 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 517 0.3199178238162422 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 515 0.31868023068735923 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 515 0.31868023068735923 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 455 0.2815524368208708 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 442 0.2735080814831316 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 425 0.26298853988762655 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 424 0.262369743323185 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 424 0.262369743323185 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 379 0.2345238979233187 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 367 0.22709833915002106 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 324 0.20049008687903763 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 324 0.20049008687903763 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 318 0.1967773074923888 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 286 0.17697581743026164 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 270 0.16707507239919805 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 263 0.1627434964481077 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 259 0.16026831019034182 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 218 0.13489765104824136 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 217 0.13427885448379992 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 206 0.1274720922749437 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 199 0.12314051632385337 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 197 0.12190292319497043 No Hit GCTTATGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATAC 172 0.10643300908393356 No Hit GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG 170 0.10519541595505062 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 169 0.10457661939060914 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 165 0.10210143313284326 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 165 0.10210143313284326 No Hit TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA 163 0.10086384000396031 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 162 0.10024504343951882 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0012375931288829485 0.0 24-25 0.0 0.0 0.0 0.006497363926635479 0.0 26-27 0.0 0.0 0.0 0.011138338159946536 0.0 28-29 0.0 0.0 0.0 0.022895472884334547 0.0 30-31 0.0 0.0 0.0 0.042387564664240984 0.0 32-33 0.0 0.0 0.0 0.05940447018638152 0.0 34-35 0.0 0.0 0.0 0.0767307739907428 0.0 36-37 0.0 0.0 0.0 0.09684166233509071 0.0 38-39 0.0 0.0 0.0 0.12406871117051559 0.0 40-41 0.0 0.0 0.0 0.1550085393925893 0.0 42-43 0.0 0.0 0.0 0.20636865424123163 0.0 44-45 0.0 0.0 0.0 0.2410212618499542 0.0 46-47 0.0 0.0 0.0 0.3199178238162422 0.0 48-49 0.0 0.0 0.0 0.4127373084824633 0.0 50-51 0.0 0.0 0.0 0.46409742333110565 0.0 52-53 0.0 0.0 0.0 0.5309274522907849 0.0 54-55 0.0 0.0 0.0 0.6299349026014207 0.0 56-57 0.0 0.0 0.0 0.7710205192940769 0.0 58-59 0.0 0.0 0.0 0.9359298037177297 0.0 60-61 0.0 0.0 0.0 1.1460112373456104 0.0 62-63 0.0 0.0 0.0 1.351142298457959 0.0 64-65 0.0 0.0 0.0 1.5114106086483008 0.0 66-67 0.0 0.0 0.0 1.7610950224004356 0.0 68-69 0.0 0.0 0.0 2.0528576025345906 0.0 70-71 0.0 0.0 0.0 2.2013687780005444 0.0 72-73 0.0 0.0 0.0 2.4538377762926658 0.0 74-75 0.0 0.0 0.0 2.6991906140937107 0.0 76-77 0.0 0.0 0.0 2.900918294101631 0.0 78-79 0.0 0.0 0.0 3.1515309027004283 0.0 80-81 0.0 0.0 0.0 3.5376599589119078 0.0 82-83 0.0 0.0 0.0 3.9550382416276824 0.0 84-85 0.0 0.0 0.0 4.647471597237692 0.0 86-87 0.0 0.0 0.0 5.590517561446498 0.0 88-89 0.0 0.0 0.0 6.360609885893913 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGACTG 15 6.6075503E-4 95.0 5 GTCACTA 25 5.2616204E-5 76.00001 1 CCCTATA 45 1.5101252E-5 52.777782 2 ACTACCT 40 5.374361E-4 47.500004 4 ACTATAT 45 9.600498E-4 42.222225 8 CCTATAC 70 4.2871943E-6 40.714287 3 CCCCTAT 50 0.0016117085 38.000004 1 ATATCAG 25 0.0016140973 38.000004 60-61 TACCTCC 90 5.6576573E-7 36.944447 2 CTACCTC 95 8.6385626E-7 35.0 1 ACTATCA 30 0.0039467886 31.666668 62-63 TACTTAT 75 3.0668394E-4 31.666668 7 CCAATAC 60 0.0039409995 31.666668 3 CGAGCAT 30 0.0039467886 31.666668 88-89 CCGGGTC 105 1.8883329E-6 31.666666 9 TCCCCGG 110 2.714325E-6 30.227274 6 GGGATGT 110 2.714325E-6 30.227274 7 CTATATC 65 0.0058294167 29.23077 9 TCACTAC 65 0.0058294167 29.23077 2 GGGATAT 65 0.0058294167 29.23077 7 >>END_MODULE