##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936957_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 161604 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.06883492982847 31.0 31.0 34.0 31.0 34.0 2 32.130671270513105 33.0 31.0 34.0 30.0 34.0 3 32.15260142075691 33.0 31.0 34.0 30.0 34.0 4 35.88880844533551 37.0 35.0 37.0 35.0 37.0 5 35.837819608425534 37.0 35.0 37.0 35.0 37.0 6 35.96416549095319 37.0 35.0 37.0 35.0 37.0 7 35.91021261849954 37.0 35.0 37.0 35.0 37.0 8 35.92676542659835 37.0 35.0 37.0 35.0 37.0 9 37.50383653869954 39.0 37.0 39.0 35.0 39.0 10-11 37.429559293086804 39.0 37.0 39.0 34.5 39.0 12-13 37.296675824855825 39.0 37.0 39.0 34.0 39.0 14-15 38.11329855696641 40.0 37.0 41.0 33.0 41.0 16-17 38.11015816440187 40.0 37.0 41.0 33.5 41.0 18-19 38.41790425979555 40.0 37.5 41.0 34.0 41.0 20-21 38.52189611643276 40.0 38.0 41.0 34.0 41.0 22-23 38.40187124081088 40.0 37.5 41.0 34.0 41.0 24-25 38.210294918442614 40.0 37.0 41.0 34.0 41.0 26-27 37.84448404742457 40.0 36.5 41.0 33.5 41.0 28-29 37.59111779411401 39.5 36.0 41.0 33.0 41.0 30-31 37.23796440682162 39.0 35.0 41.0 33.0 41.0 32-33 36.709645182049954 38.0 35.0 41.0 33.0 41.0 34-35 36.375751837825796 38.0 35.0 40.0 32.0 41.0 36-37 36.01095888715626 38.0 35.0 40.0 31.0 41.0 38-39 35.65896883740501 38.0 35.0 40.0 30.5 41.0 40-41 35.252923813766984 37.0 35.0 40.0 30.0 41.0 42-43 34.79489987871587 37.0 35.0 40.0 27.0 41.0 44-45 34.20737110467563 37.0 34.0 40.0 23.5 41.0 46-47 33.318213039281204 37.0 33.0 40.0 17.5 41.0 48-49 32.58027029033934 36.0 33.0 40.0 5.0 41.0 50-51 32.174964728595825 36.0 33.0 40.0 2.0 41.0 52-53 31.81173114526868 35.0 32.0 40.0 2.0 41.0 54-55 31.517202544491475 35.0 32.0 39.5 2.0 41.0 56-57 31.2148771070023 35.0 31.0 39.0 2.0 41.0 58-59 30.89515420410386 35.0 31.0 39.0 2.0 41.0 60-61 30.49431944753843 35.0 30.5 39.0 2.0 40.0 62-63 30.033804856315438 35.0 29.5 38.0 2.0 40.0 64-65 29.490495284770176 35.0 28.5 38.0 2.0 40.0 66-67 29.064116606024605 35.0 28.0 37.0 2.0 39.5 68-69 28.820270537857972 35.0 28.0 37.0 2.0 39.0 70-71 28.335644538501523 34.0 27.0 36.0 2.0 39.0 72-73 27.78382342021237 34.0 26.0 35.5 2.0 38.5 74-75 27.251624340981657 33.5 25.5 35.0 2.0 37.5 76-77 25.82046545877577 31.5 23.5 34.5 2.0 36.0 78-79 26.430478205985 33.0 24.0 35.0 2.0 36.0 80-81 26.16110987351798 33.0 22.5 35.0 2.0 36.0 82-83 25.827643498923294 33.0 20.0 35.0 2.0 36.0 84-85 25.467107868617113 33.0 19.0 35.0 2.0 35.0 86-87 25.04535778817356 33.0 14.0 35.0 2.0 35.0 88-89 24.497035964456323 32.5 4.5 35.0 2.0 35.0 90-91 23.89092163560308 32.0 2.0 35.0 2.0 35.0 92-93 23.40262926660231 31.5 2.0 34.0 2.0 35.0 94-95 22.777035221900448 31.0 2.0 34.0 2.0 35.0 96-97 22.01439630207173 31.0 2.0 34.0 2.0 35.0 98-99 21.259254102621224 30.5 2.0 34.0 2.0 35.0 100-101 18.421242667260714 24.5 2.0 32.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.14787128666808513 1101 2 0.06452476813038288 1101 3 0.0740323034507071 1101 4 0.16081872554315169 1101 5 0.10527309137739138 1101 6 0.25050020172606935 1101 7 0.15115676078650608 1101 8 0.23701149236167396 1101 9 0.14983649217236916 1101 10-11 0.1736755188365251 1101 12-13 0.2119580527449898 1101 14-15 0.2240737046105039 1101 16-17 0.32690072466444775 1101 18-19 0.3943140054787335 1101 20-21 0.538286882039273 1101 22-23 0.6624028081701923 1101 24-25 0.6716002546757522 1101 26-27 0.35335543380185186 1101 28-29 0.47014884599730067 1101 30-31 0.6197693997979385 1101 32-33 0.4826873831556284 1101 34-35 0.737337806505117 1101 36-37 0.9859575471574828 1101 38-39 1.1347910176198894 1101 40-41 1.0537992303992283 1101 42-43 0.975882515171719 1101 44-45 1.5079976786077367 1101 46-47 1.904020187685866 1101 48-49 1.8749348187748538 1101 50-51 1.9152963203636233 1101 52-53 1.8509830295498908 1101 54-55 1.8230978546383163 1101 56-57 1.8687453440597324 1101 58-59 1.8751029852492422 1101 60-61 2.25514325109717 1101 62-63 1.9371523449856305 1101 64-65 1.7630896687767645 1101 66-67 1.4605527352588012 1101 68-69 1.5753080833138178 1101 70-71 1.415887563504949 1101 72-73 1.0886315665108626 1101 74-75 0.9390612421799638 1101 76-77 1.123068051751364 1101 78-79 1.3087406853667574 1101 80-81 1.7238361904045583 1101 82-83 1.7090993931266176 1101 84-85 1.7549591685849677 1101 86-87 1.6377313319403832 1101 88-89 1.4716426930454283 1101 90-91 1.7203539741429559 1101 92-93 1.7758430927629938 1101 94-95 1.6634285944646265 1101 96-97 1.8070932794878054 1101 98-99 1.5947801692597423 1101 100-101 1.5176722107361194 1102 1 0.2700874735230556 1102 2 0.21071645139431183 1102 3 0.24106109215249916 1102 4 0.13536456415743459 1102 5 -0.1146406028708995 1102 6 -0.002608012408764182 1102 7 0.01425954309080879 1102 8 0.2476186503072313 1102 9 0.3275177040741113 1102 10-11 -0.07424227946149387 1102 12-13 0.06091544305218832 1102 14-15 0.08292155300208037 1102 16-17 -0.0888002832418806 1102 18-19 0.045643295116008176 1102 20-21 0.32218301336887123 1102 22-23 0.3863601984773908 1102 24-25 0.4947338173530369 1102 26-27 0.4974724595728617 1102 28-29 0.5817503306976448 1102 30-31 0.7060374304576129 1102 32-33 0.8523309947194022 1102 34-35 0.9541055655732649 1102 36-37 0.3040591223883382 1102 38-39 0.1924363190864824 1102 40-41 0.3757408080442346 1102 42-43 0.31012869780192887 1102 44-45 0.24705857546330634 1102 46-47 0.225841057985825 1102 48-49 0.47555947928544384 1102 50-51 0.3840859274612143 1102 52-53 0.49512268794810765 1102 54-55 0.6152548948942176 1102 56-57 0.6212575656784196 1102 58-59 1.003633675091713 1102 60-61 1.228248145780011 1102 62-63 1.4101289881143622 1102 64-65 1.5377894092559714 1102 66-67 1.1427001189270989 1102 68-69 1.0480961212159237 1102 70-71 1.286044181285142 1102 72-73 1.4392185059761253 1102 74-75 1.2274386336942342 1102 76-77 1.1418154112781913 1102 78-79 1.1716095971900415 1102 80-81 1.4894225263330974 1102 82-83 1.4728902072976595 1102 84-85 1.6393064436166505 1102 86-87 1.7243690288442366 1102 88-89 1.9464827558132498 1102 90-91 1.9926772887666502 1102 92-93 2.5122031627732575 1102 94-95 2.295331545759211 1102 96-97 2.2448610989096203 1102 98-99 2.7847056324741786 1102 100-101 1.8795754637990676 1103 1 0.2914271297174835 1103 2 0.3412077903918771 1103 3 0.40259043417977125 1103 4 0.10758678637965602 1103 5 0.07690586357183093 1103 6 0.08632368751879227 1103 7 0.0764837412071202 1103 8 0.23014845337645795 1103 9 0.3637424290955167 1103 10-11 0.4186938991593294 1103 12-13 0.389860644922706 1103 14-15 0.4434069643173615 1103 16-17 0.6318247579225726 1103 18-19 0.7232350095334539 1103 20-21 0.5635302438239762 1103 22-23 0.70752531736035 1103 24-25 0.5361617301502406 1103 26-27 0.5406375125926886 1103 28-29 0.28445236586856026 1103 30-31 0.39146685810695914 1103 32-33 0.6021910355545401 1103 34-35 0.6284205889546755 1103 36-37 0.15742308208010058 1103 38-39 0.28231480159982425 1103 40-41 0.3660753927771552 1103 42-43 0.43422307967639284 1103 44-45 0.45400055004014206 1103 46-47 0.5521435015081906 1103 48-49 0.3903490465646371 1103 50-51 0.4579102704764324 1103 52-53 0.41590580063993343 1103 54-55 0.18926859442658994 1103 56-57 -0.09280586052193485 1103 58-59 -0.35817723184367267 1103 60-61 -0.5106456828441068 1103 62-63 -0.23554380382399032 1103 64-65 -0.4632644009264695 1103 66-67 -0.30997721756766694 1103 68-69 -0.44840489966269104 1103 70-71 -0.40751934370991805 1103 72-73 0.07699595443822105 1103 74-75 0.12305379538823402 1103 76-77 0.3199504965709501 1103 78-79 0.3290142603000987 1103 80-81 0.18796695101860195 1103 82-83 0.3458237513930307 1103 84-85 0.6396522250827665 1103 86-87 0.49111473127458893 1103 88-89 0.7945447330246047 1103 90-91 1.072017669455576 1103 92-93 1.0128782599213828 1103 94-95 1.1176986668815196 1103 96-97 1.336699009730932 1103 98-99 0.9069064491770682 1103 100-101 0.7102001756478451 1104 1 -0.09118236774678579 1104 2 -0.08944227876441602 1104 3 -0.18274843165702137 1104 4 0.049809008601876315 1104 5 -0.1811485393788388 1104 6 -0.19990959971035238 1104 7 -0.3122483934171285 1104 8 -0.06619087350229336 1104 9 -0.06168864513223582 1104 10-11 0.1694085141892927 1104 12-13 -0.26678296964622206 1104 14-15 -0.26549114541062124 1104 16-17 0.1710409865993867 1104 18-19 0.2281036125763336 1104 20-21 -0.014404288218436534 1104 22-23 -0.10744932533213358 1104 24-25 0.19759096021017797 1104 26-27 -0.41974976264936004 1104 28-29 -0.21563062168330305 1104 30-31 -0.4203911409709562 1104 32-33 -0.5329864655980643 1104 34-35 -0.5185134820457833 1104 36-37 -0.74570278237357 1104 38-39 -0.9262144745643042 1104 40-41 -1.4644179221144995 1104 42-43 -1.3628078101345764 1104 44-45 -1.032306503901772 1104 46-47 -0.9708256086808404 1104 48-49 -0.920789727063763 1104 50-51 -0.3442474058721139 1104 52-53 -0.45233762951221124 1104 54-55 -0.49823716859784284 1104 56-57 -0.9273932279723738 1104 58-59 -1.1140647376066966 1104 60-61 -1.3832597907279265 1104 62-63 -1.2211408531554788 1104 64-65 -1.183162971696408 1104 66-67 -1.10460146837449 1104 68-69 -0.7645229264031244 1104 70-71 -0.9135589933180341 1104 72-73 -0.6122894305318063 1104 74-75 -0.9829581917025898 1104 76-77 -1.0676290331662521 1104 78-79 -0.9891046885242432 1104 80-81 -0.7178790609684889 1104 82-83 -0.4221891577817054 1104 84-85 -0.6151380008277911 1104 86-87 -0.9715039870287754 1104 88-89 -1.0588347045042106 1104 90-91 -0.9043862032968413 1104 92-93 -0.2209714404013461 1104 94-95 -0.15228750185983486 1104 96-97 0.02287697192549132 1104 98-99 -0.3166435738750266 1104 100-101 0.1423532415768456 1105 1 0.15913046936216801 1105 2 0.1981952616886531 1105 3 -0.18404293003889904 1105 4 0.06389746599130319 1105 5 0.04135415749279048 1105 6 4.355998581502263E-4 1105 7 0.1152381222247314 1105 8 -0.060710830352341816 1105 9 0.053284386151474905 1105 10-11 0.01741282918389686 1105 12-13 0.06232166897297731 1105 14-15 0.13316041877492069 1105 16-17 -0.044956855903308224 1105 18-19 0.25913921128183404 1105 20-21 5.040181461879456E-4 1105 22-23 -0.1789811484173569 1105 24-25 0.08554133777221296 1105 26-27 0.18989425029562312 1105 28-29 0.14029170841378402 1105 30-31 0.5818850186838418 1105 32-33 0.6087071697848927 1105 34-35 0.34729018569962733 1105 36-37 0.4353004538723866 1105 38-39 0.3503766796967511 1105 40-41 0.8200696722760128 1105 42-43 1.4039990938567897 1105 44-45 1.7623536902729882 1105 46-47 1.4755713708229408 1105 48-49 1.661065720616925 1105 50-51 1.9208982251958417 1105 52-53 2.1647173866690146 1105 54-55 2.155862076278101 1105 56-57 2.0898775379391736 1105 58-59 2.3297432451333293 1105 60-61 2.140041180145861 1105 62-63 2.3234438286136694 1105 64-65 2.6217776971277544 1105 66-67 1.9788073773644541 1105 68-69 1.9493713562290225 1105 70-71 1.616430219195454 1105 72-73 1.7825110009676521 1105 74-75 2.378713868707333 1105 76-77 2.0934065655392473 1105 78-79 2.092610521831741 1105 80-81 2.4212687275967752 1105 82-83 2.3435174225850695 1105 84-85 2.729921330348045 1105 86-87 2.3932317195515864 1105 88-89 1.820981908225022 1105 90-91 2.0900150912015434 1105 92-93 2.3016930687680137 1105 94-95 2.0211752813116846 1105 96-97 2.082952484331102 1105 98-99 1.5226660269879702 1105 100-101 1.2146617636911934 1106 1 0.23980797041746627 1106 2 0.08744177920659979 1106 3 0.1882660610965985 1106 4 -0.07176103970729741 1106 5 0.06720894854386472 1106 6 0.36963146309158645 1106 7 0.3011815582736972 1106 8 0.05774632456534334 1106 9 -0.009611350446242284 1106 10-11 0.18106310356127153 1106 12-13 0.1850527791460479 1106 14-15 0.27913445845410934 1106 16-17 0.4567052378071139 1106 18-19 0.06237897489516797 1106 20-21 -0.06877626889476574 1106 22-23 -0.21232855238527293 1106 24-25 0.014704486780232173 1106 26-27 0.11401835329267129 1106 28-29 0.2869780739688679 1106 30-31 0.251154752748846 1106 32-33 -0.33383187622607835 1106 34-35 -0.3664603419491712 1106 36-37 -0.37334770990979393 1106 38-39 -0.39801399147252425 1106 40-41 -0.3586449752545988 1106 42-43 -0.2461169888785335 1106 44-45 0.004650017837363407 1106 46-47 -0.22947295167600856 1106 48-49 -0.34975107972076813 1106 50-51 -0.460116971089505 1106 52-53 -0.305127484584677 1106 54-55 -0.06659465652054664 1106 56-57 -0.18114926661971253 1106 58-59 -0.13611787770331318 1106 60-61 -0.07782500811923043 1106 62-63 -0.8130852975999225 1106 64-65 -1.1046364016481007 1106 66-67 -0.8597584732054067 1106 68-69 -0.7815277573209976 1106 70-71 -0.9570976406610292 1106 72-73 -0.7747652759424355 1106 74-75 -0.10218524484268343 1106 76-77 0.3504506769786708 1106 78-79 0.04984458683807347 1106 80-81 0.08267649458706572 1106 82-83 0.4870378953583909 1106 84-85 0.5894876030369431 1106 86-87 0.5469621965461009 1106 88-89 0.5193778558822615 1106 90-91 0.825794956123449 1106 92-93 0.291878801144545 1106 94-95 -0.6752947482366451 1106 96-97 -0.3235481488474541 1106 98-99 -0.15322569947889164 1106 100-101 0.9985368164560704 1107 1 -0.015626812191229078 1107 2 0.18458070974132568 1107 3 -0.05861050062254236 1107 4 0.06735690132218508 1107 5 0.0915717671345746 1107 6 0.008749404121068949 1107 7 0.1744949016020314 1107 8 0.28606966289617475 1107 9 0.17179794490608202 1107 10-11 0.39086445288661054 1107 12-13 0.25214423341891035 1107 14-15 0.14217168983842043 1107 16-17 0.3026884961779288 1107 18-19 0.022586371197022004 1107 20-21 -0.2385039050766693 1107 22-23 0.1652326670050357 1107 24-25 0.1184338720875786 1107 26-27 0.17611230631615626 1107 28-29 0.4641394932592249 1107 30-31 0.3592257172665896 1107 32-33 0.20858441562798902 1107 34-35 0.28914935320324986 1107 36-37 0.5961362980862077 1107 38-39 0.3753180924855002 1107 40-41 0.5258502771192184 1107 42-43 0.7135906572983757 1107 44-45 0.9780383236844372 1107 46-47 1.1693276480241437 1107 48-49 0.7510110392197618 1107 50-51 0.6585112148175369 1107 52-53 -0.11256751456967962 1107 54-55 0.28547930649794395 1107 56-57 0.571380718387779 1107 58-59 0.6450540363396655 1107 60-61 0.6236367609962095 1107 62-63 0.7594721736644452 1107 64-65 0.16928913558328418 1107 66-67 0.3734061944607596 1107 68-69 0.5555537019493642 1107 70-71 0.41268621740993794 1107 72-73 0.6669826001195318 1107 74-75 0.6716778236230816 1107 76-77 -0.22073248144211632 1107 78-79 0.6200907137746086 1107 80-81 0.8944581037824655 1107 82-83 0.5666231027546935 1107 84-85 0.5847087424672246 1107 86-87 1.0609864344271607 1107 88-89 0.64181663649196 1107 90-91 1.2951540265882144 1107 92-93 1.1437028891005703 1107 94-95 1.2735745671056833 1107 96-97 1.2246777382090173 1107 98-99 0.6928200276575396 1107 100-101 0.6377172262511728 1108 1 0.036097325739802955 1108 2 -0.07978710635062214 1108 3 0.0793205338602192 1108 4 -0.08781551247091812 1108 5 0.011111997019632724 1108 6 -0.014239101626060346 1108 7 0.0020811084985794537 1108 8 0.21135701921801342 1108 9 0.2062807035267653 1108 10-11 0.10638169426592725 1108 12-13 -0.13578680106385121 1108 14-15 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2316 94-95 0.23859791480683157 2316 96-97 1.2966964163699366 2316 98-99 0.8162036483471944 2316 100-101 1.500235186021289 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 1.0 5 12.0 6 32.0 7 77.0 8 127.0 9 240.0 10 405.0 11 659.0 12 945.0 13 1289.0 14 1643.0 15 2343.0 16 3185.0 17 4333.0 18 3724.0 19 2127.0 20 1742.0 21 1842.0 22 1865.0 23 2092.0 24 2232.0 25 2487.0 26 2643.0 27 3155.0 28 3638.0 29 4228.0 30 5034.0 31 5925.0 32 7076.0 33 9004.0 34 13106.0 35 18902.0 36 19539.0 37 22257.0 38 12989.0 39 705.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.03223011073491 19.66983789003262 17.15246012243357 14.1454718767989 2 15.245143843711903 25.960556686613163 39.60420547156851 19.190093998106423 3 20.44875126853296 32.949679463379624 33.070344793445706 13.531224474641718 4 10.830177470854682 19.994554590232916 38.290512611073986 30.88475532783842 5 9.93477884210787 42.34239251503676 32.007871092299695 15.714957550555681 6 22.678275290215588 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14.330844608915472 28-29 16.01321749461647 27.201987574564985 42.045679562387065 14.739115368431474 30-31 15.743551186730157 29.525462640664113 40.37543432993295 14.355551842672782 32-33 15.365074846686511 29.801295816135198 39.56849446462496 15.265134872553327 34-35 16.237531249226507 28.149983911289322 39.71343531100715 15.899049528477018 36-37 17.358829171694666 28.26417896593335 39.80321173303629 14.573780129335685 38-39 18.153884238701593 27.457181316045983 40.163693469280396 14.225240975972026 40-41 15.817368381970741 28.846748842850424 40.655862478651514 14.680020296527314 42-43 15.088735427340907 30.59144575629316 38.80566075097151 15.51415806539442 44-45 15.836860473750649 31.01098982698448 36.96907254770922 16.183077151555654 46-47 16.182867892303978 30.716632735778422 35.16364176407617 17.93685760784144 48-49 17.211793426300368 30.405604012413484 35.035597483887535 17.347005077398613 50-51 18.329666657383942 31.380557644168437 34.55122671196636 15.738548986481259 52-53 16.860040593054627 31.89339372787802 34.86671122001931 16.379854459048044 54-55 15.255758115818265 31.377863587420634 35.41689872399411 17.94947957276699 56-57 17.378981729296555 32.79227710824734 32.39035257352372 17.438388588932376 58-59 16.781814750299343 31.268815341251894 33.520623006308654 18.428746902140112 60-61 16.900375625785134 30.477050935351524 34.36821228116859 18.25436115769476 62-63 19.550237783615362 30.047989900771366 33.95741863221048 16.444353683402795 64-65 18.588339397539666 30.725105814212522 34.711702680626715 15.974852107621098 66-67 17.021915154965487 30.767231537031137 34.99308479862377 17.217768509379614 68-69 16.976275046100913 32.42719768319699 34.120555438670316 16.47597183203178 70-71 17.54206295675204 31.9718693341089 32.60985253986621 17.876215169272847 72-73 16.725761738570828 31.247060716318902 32.35563476151581 19.671542783594465 74-75 16.972970966065194 32.096359001014825 32.911314076384244 18.01935595653573 76-77 17.916635726838447 30.301848964134553 33.66222370733398 18.11929160169303 78-79 16.4299725868033 30.226916912642864 34.608696728361835 18.734413772192006 80-81 16.33508663366337 32.46441831683168 33.00030940594059 18.200185643564357 82-83 16.15789978555979 31.620493429093937 32.2956861313191 19.925920654027173 84-85 16.416446460508322 30.041277051320925 32.233540234295226 21.308736253875527 86-87 17.024449711318898 30.10699456054258 32.68470330513561 20.1838524230029 88-89 17.782356872354647 28.754548154748644 33.112732358109945 20.350362614786764 90-91 16.083390315065145 28.991067533407794 34.20203401577337 20.72350813575369 92-93 15.49285751855622 30.671984208262813 33.24061669456416 20.5945415786168 94-95 15.44268706220143 31.121754412019502 32.24610776960966 21.1894507561694 96-97 15.200851487783067 30.170885429719768 31.447498290526887 23.180764791970272 98-99 16.040123514087163 29.980940712000542 31.360263859753346 22.61867191415895 100-101 16.99591902403104 29.899104908000133 31.39721602564301 21.707760042325816 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2828.0 1 1928.0 2 1130.5 3 1157.0 4 921.5 5 901.5 6 1570.5 7 2963.5 8 4084.5 9 4084.0 10 3042.0 11 2090.0 12 1870.0 13 1703.0 14 1472.0 15 1298.5 16 1169.5 17 1090.5 18 1031.0 19 942.5 20 900.0 21 902.5 22 944.5 23 1009.5 24 1058.5 25 1150.5 26 1282.0 27 1370.0 28 1457.5 29 1656.0 30 1895.0 31 2050.0 32 2366.5 33 2718.5 34 3057.5 35 3289.0 36 3586.0 37 3756.0 38 3662.0 39 3579.0 40 3348.0 41 3219.0 42 3094.0 43 3133.5 44 3534.0 45 3785.5 46 3893.5 47 5062.0 48 12534.5 49 12179.5 50 4563.5 51 3015.5 52 2109.0 53 1865.5 54 1748.5 55 1710.5 56 1694.0 57 1616.5 58 1669.5 59 1836.5 60 1937.0 61 1824.5 62 1462.0 63 928.5 64 502.0 65 369.5 66 334.5 67 256.5 68 185.5 69 142.5 70 98.0 71 77.0 72 77.0 73 70.0 74 54.5 75 37.5 76 23.0 77 14.0 78 13.0 79 13.0 80 8.0 81 2.5 82 3.0 83 2.5 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02908343852874929 2 0.003093982822207371 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.012685329571050221 12-13 0.0 14-15 0.0 16-17 0.0 18-19 6.187965644414743E-4 20-21 0.0015469914111036855 22-23 0.0012375931288829485 24-25 0.008044355337739166 26-27 0.005259770797752531 28-29 0.0 30-31 0.002784584539986634 32-33 0.004331575951090319 34-35 0.0 36-37 0.005569169079973268 38-39 0.013304126135491695 40-41 0.0 42-43 0.0 44-45 0.0 46-47 6.187965644414743E-4 48-49 0.003403381104428108 50-51 0.008353753619959901 52-53 0.0 54-55 0.0037127793866488456 56-57 0.004022177668869583 58-59 3.0939828222073713E-4 60-61 0.004331575951090319 62-63 0.004640974233311056 64-65 0.0 66-67 0.0021657879755451596 68-69 0.0012375931288829485 70-71 6.187965644414743E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0018563896933244228 80-81 0.002475186257765897 82-83 0.012685329571050221 84-85 0.008044355337739166 86-87 0.004331575951090319 88-89 0.0 90-91 0.0015469914111036855 92-93 3.0939828222073713E-4 94-95 0.0 96-97 0.002784584539986634 98-99 0.0018563896933244228 100-101 3.0939828222073713E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 161604.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.564589985396395 #Duplication Level Percentage of deduplicated Percentage of total 1 77.83049089300424 31.571619514368454 2 11.717057692894407 9.505952822949927 3 3.9112792506940846 4.75978317368382 4 1.944961405863868 3.155862478651519 5 1.101382066693108 2.2338555976337218 6 0.7001861061109924 1.70416573847182 7 0.433230619031638 1.2301675701096506 8 0.34933032309241235 1.1336353060567808 9 0.3127192848643866 1.14167966139452 >10 1.4873234280135461 11.072126927551299 >50 0.10373127497940629 2.8662656864929086 >100 0.0732220764560515 5.751095269919062 >500 0.021356438966348354 6.5716195143684555 >1k 0.012203679409341917 13.901264820177719 >5k 0.0015254599261677396 3.4009059181703423 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5496 3.4009059181703423 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4388 2.715279324769189 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3645 2.2555134773891736 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3413 2.1119526744387516 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3215 1.9894309546793398 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2854 1.7660453949159673 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2354 1.4566471126952303 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1422 0.8799287146357764 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1174 0.7264671666542907 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 929 0.5748620083661296 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 924 0.5717680255439223 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 885 0.5476349595307047 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 863 0.5340214351129923 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 846 0.5235018935174872 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 841 0.5204079106952798 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 823 0.5092695725353332 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 764 0.4727605752332863 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 691 0.42758842602905867 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 686 0.42449444320685137 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 663 0.41026212222469743 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 590 0.3650899730204698 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 572 0.3539516348605233 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 543 0.3360065344917205 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 475 0.29392836810970024 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 407 0.25185020172767997 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 390 0.24133066013217494 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 350 0.21657879755451595 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 315 0.1949209177990644 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 312 0.19306452810573996 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 282 0.17450063117249573 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 272 0.168312665528081 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 270 0.16707507239919805 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 260 0.1608871067547833 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 257 0.15903071706145885 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 246 0.15222395485260265 No Hit GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG 246 0.15222395485260265 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 245 0.15160515828816118 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 217 0.13427885448379992 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 216 0.13366005791935842 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 209 0.1293284819682681 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 203 0.12561570258161928 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 201 0.12437810945273632 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 194 0.120046533501646 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 191 0.11819014380832157 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 184 0.11385856785723127 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 177 0.10952699190614093 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 167 0.10333902626172621 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 3.0939828222073713E-4 0.0 22-23 0.0 0.0 0.0 0.0018563896933244226 0.0 24-25 0.0 0.0 0.0 0.007116160491076953 0.0 26-27 0.0 0.0 0.0 0.01175713472438801 0.0 28-29 0.0 0.0 0.0 0.023823667730996756 0.0 30-31 0.0 0.0 0.0 0.04393455607534467 0.0 32-33 0.0 0.0 0.0 0.06095146159748521 0.0 34-35 0.0 0.0 0.0 0.07858716368406723 0.0 36-37 0.0 0.0 0.0 0.10024504343951882 0.0 38-39 0.0 0.0 0.0 0.12809088883938516 0.0 40-41 0.0 0.0 0.0 0.16057770847256256 0.0 42-43 0.0 0.0 0.0 0.2125566198856464 0.0 44-45 0.0 0.0 0.0 0.24751862577658967 0.0 46-47 0.0 0.0 0.0 0.3273433825895399 0.0 48-49 0.0 0.0 0.0 0.4241850449246306 0.0 50-51 0.0 0.0 0.0 0.4764733546199351 0.0 52-53 0.0 0.0 0.0 0.543612781861835 0.0 54-55 0.0 0.0 0.0 0.6450954184302369 0.0 56-57 0.0 0.0 0.0 0.7880374248162174 0.0 58-59 0.0 0.0 0.0 0.9569688869087398 0.0 60-61 0.0 0.0 0.0 1.169834905076607 0.0 62-63 0.0 0.0 0.0 1.373418974777852 0.0 64-65 0.0 0.0 0.0 1.537090666072622 0.0 66-67 0.0 0.0 0.0 1.786465681542536 0.0 68-69 0.0 0.0 0.0 2.0816316427811192 0.0 70-71 0.0 0.0 0.0 2.2326180045048387 0.0 72-73 0.0 0.0 0.0 2.486633994208064 0.0 74-75 0.0 0.0 0.0 2.733224425137992 0.0 76-77 0.0 0.0 0.0 2.9324769188881463 0.0 78-79 0.0 0.0 0.0 3.1793767481002946 0.0 80-81 0.0 0.0 0.0 3.5596272369495807 0.0 82-83 0.0 0.0 0.0 3.9794807059231205 0.0 84-85 0.0 0.0 0.0 4.681196009999752 0.0 86-87 0.0 0.0 0.0 5.630429939852974 0.0 88-89 0.0 0.0 0.0 6.415063983564764 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTATC 20 0.0020658155 71.293655 1 GGGGACG 20 0.002070896 71.249535 5 CTGTATA 20 0.002070896 71.249535 9 TAGATAG 20 0.002070896 71.249535 5 CCTTAGG 25 0.005013621 56.999626 2 TATATGG 25 0.005013621 56.999626 2 AGGGATC 25 0.005013621 56.999626 6 GACGTAT 35 2.7808503E-4 54.285366 8 GGGATAT 55 0.0025731805 34.545227 7 AGACGTA 60 0.0039411252 31.666458 7 ACTACGT 30 0.0039469143 31.666458 26-27 TTCGACC 30 0.0039469143 31.666458 16-17 TGGGATA 110 2.7144652E-6 30.227074 6 CCTCTAG 65 0.005811983 29.248678 1 GAGACGT 65 0.0058296 29.23058 6 ATAGCTG 70 0.008370884 27.142683 5 TTTCCCG 35 0.008383069 27.142683 74-75 ATGGGAG 330 0.0 25.908922 5 GAGTACT 3030 0.0 25.23911 12-13 AGTACTT 3060 0.0 24.83644 12-13 >>END_MODULE