##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936956_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 166236 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.890908106547318 33.0 31.0 34.0 30.0 34.0 2 31.87896725137756 33.0 31.0 34.0 30.0 34.0 3 31.804079742053467 33.0 31.0 34.0 30.0 34.0 4 35.491036839192475 37.0 35.0 37.0 33.0 37.0 5 35.511062585721504 37.0 35.0 37.0 33.0 37.0 6 35.680003128082966 37.0 35.0 37.0 35.0 37.0 7 35.66993310714887 37.0 35.0 37.0 33.0 37.0 8 35.64450540195866 37.0 35.0 37.0 33.0 37.0 9 37.20418561563079 39.0 37.0 39.0 34.0 39.0 10-11 37.109034745783106 39.0 37.0 39.0 33.5 39.0 12-13 36.997178709786084 39.0 37.0 39.0 33.0 39.0 14-15 37.82625604562189 40.0 37.5 41.0 32.5 41.0 16-17 37.85450203325393 40.0 37.5 41.0 32.5 41.0 18-19 38.00829543540509 40.0 37.5 41.0 33.5 41.0 20-21 38.068387713852594 40.0 37.5 41.0 34.0 41.0 22-23 37.96714911330879 40.0 37.0 41.0 33.5 41.0 24-25 37.84503958228061 40.0 37.0 41.0 33.0 41.0 26-27 37.68563367742246 40.0 36.5 41.0 33.0 41.0 28-29 37.486004836497514 39.5 36.0 41.0 33.0 41.0 30-31 37.10142809018504 39.0 35.5 41.0 33.0 41.0 32-33 36.60014076373349 38.0 35.0 41.0 32.5 41.0 34-35 36.278107630116224 38.0 35.0 40.0 31.5 41.0 36-37 35.92771722129984 38.0 35.0 40.0 31.0 41.0 38-39 35.566847734546066 38.0 35.0 40.0 30.5 41.0 40-41 35.16460333501769 37.0 35.0 40.0 30.0 41.0 42-43 34.75828039654468 37.0 35.0 40.0 28.0 41.0 44-45 34.18396436391636 37.0 35.0 40.0 24.0 41.0 46-47 33.439303159363796 36.5 33.5 40.0 17.5 41.0 48-49 32.77842043841286 36.0 33.0 40.0 7.0 41.0 50-51 31.806200221372023 35.5 32.0 39.0 2.0 40.5 52-53 31.884017300705022 35.0 32.5 39.0 2.0 40.5 54-55 32.178478187636856 35.0 33.0 40.0 2.0 41.0 56-57 32.0290340239178 35.0 33.0 40.0 2.0 41.0 58-59 31.805385115137515 35.0 33.0 40.0 2.0 41.0 60-61 31.467747659953318 35.0 32.5 39.5 2.0 41.0 62-63 31.074090449722082 35.0 32.0 39.0 2.0 41.0 64-65 30.628085974157223 35.0 31.0 39.0 2.0 41.0 66-67 30.223221805144494 35.0 31.0 38.5 2.0 40.0 68-69 29.834358983613658 35.0 30.5 37.5 2.0 40.0 70-71 29.2977694362232 35.0 29.5 37.0 2.0 39.5 72-73 28.829296903197864 35.0 29.0 36.5 2.0 39.0 74-75 28.409186337496088 35.0 28.5 36.0 2.0 39.0 76-77 27.889665896677016 34.5 26.5 35.0 2.0 37.5 78-79 27.41707271589788 34.0 26.0 35.0 2.0 37.0 80-81 27.008872927645037 34.0 25.5 35.0 2.0 37.0 82-83 26.59934069635939 34.0 24.0 35.0 2.0 36.0 84-85 26.19222370605645 34.0 22.0 35.0 2.0 36.0 86-87 25.75367248971342 34.0 19.5 35.0 2.0 35.5 88-89 25.255465121874927 33.0 13.5 35.0 2.0 35.0 90-91 24.751142953391565 33.0 4.5 35.0 2.0 35.0 92-93 24.351996559108734 33.0 2.0 35.0 2.0 35.0 94-95 23.940361895137034 33.0 2.0 35.0 2.0 35.0 96-97 23.41112334271758 32.0 2.0 35.0 2.0 35.0 98-99 22.89245109362593 32.0 2.0 35.0 2.0 35.0 100-101 21.171849057965787 29.0 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3412421200729945 1101 2 0.24328106036441355 1101 3 0.015413719882602095 1101 4 0.3627871068870263 1101 5 0.5216784546194333 1101 6 0.3400666644069048 1101 7 0.43242319544707186 1101 8 0.4436780020685802 1101 9 0.507869925438662 1101 10-11 0.34738524106568036 1101 12-13 0.6041963892308218 1101 14-15 0.7436862813658038 1101 16-17 0.6920942410592872 1101 18-19 0.4928313963757489 1101 20-21 0.5618479344767238 1101 22-23 0.2228050738567262 1101 24-25 0.06524623361216442 1101 26-27 0.42274220526199 1101 28-29 0.4358630206480498 1101 30-31 0.2421996889446305 1101 32-33 0.49689054568018776 1101 34-35 0.6261652382831997 1101 36-37 0.8570281368180233 1101 38-39 1.1015893943950417 1101 40-41 1.1804624541379596 1101 42-43 1.254285606010903 1101 44-45 1.5907487614257079 1101 46-47 1.6432453367711517 1101 48-49 1.8498900349615681 1101 50-51 1.8656066223571095 1101 52-53 1.6812975598584678 1101 54-55 2.194130938751453 1101 56-57 2.0483204052511894 1101 58-59 2.254099193927047 1101 60-61 2.43412015493557 1101 62-63 2.39513021789406 1101 64-65 2.300841900237355 1101 66-67 2.5018881744697907 1101 68-69 2.431981317543567 1101 70-71 2.599801504412806 1101 72-73 2.806308046115511 1101 74-75 2.938128222548549 1101 76-77 2.8343419048814447 1101 78-79 2.711486059601249 1101 80-81 2.9155999553223815 1101 82-83 2.874571566682004 1101 84-85 2.9961179774296127 1101 86-87 2.994459736846011 1101 88-89 3.0511816035227355 1101 90-91 3.2146790023034306 1101 92-93 3.3191957052236454 1101 94-95 3.102156092399664 1101 96-97 3.010873204527364 1101 98-99 3.0641598903135474 1101 100-101 3.192588433511453 1102 1 0.3747180180695828 1102 2 0.2189700335772642 1102 3 0.3864784528150409 1102 4 0.019184830140069664 1102 5 0.17288275085380178 1102 6 -0.13975454021090172 1102 7 0.08748638482224891 1102 8 -0.14674872719790955 1102 9 0.2987661533706145 1102 10-11 0.3793015030845197 1102 12-13 0.057897710901720245 1102 14-15 -0.04044080484829493 1102 16-17 0.07458290556068192 1102 18-19 -0.17900189944057132 1102 20-21 -0.3822785085885272 1102 22-23 -0.3695109051766252 1102 24-25 -0.132280859033429 1102 26-27 -0.12589592703373143 1102 28-29 -0.502812735855791 1102 30-31 -0.4421750095793442 1102 32-33 -0.14105684474332492 1102 34-35 -0.23046884217472297 1102 36-37 -0.05301945619738291 1102 38-39 0.03738705976468992 1102 40-41 -0.4154149616899332 1102 42-43 -0.4977001160844736 1102 44-45 -0.34960722146653467 1102 46-47 -0.9135286285631281 1102 48-49 -1.0382020639547278 1102 50-51 -0.7235177304743523 1102 52-53 -0.5340527954812764 1102 54-55 -0.9307846479610902 1102 56-57 -0.8779827751199072 1102 58-59 -1.4596352499099723 1102 60-61 -1.537902869793502 1102 62-63 -1.7188839956956734 1102 64-65 -1.3008318946624762 1102 66-67 -1.2150137774869947 1102 68-69 -0.9671327449061877 1102 70-71 -1.1242715572973232 1102 72-73 -0.8890725372204997 1102 74-75 -1.0805070155773144 1102 76-77 -1.2132752286041004 1102 78-79 -1.0588615623558582 1102 80-81 -0.8707138370048497 1102 82-83 -0.5711064730002811 1102 84-85 -0.45878120898708374 1102 86-87 -0.8534998734057808 1102 88-89 -0.5076584775140205 1102 90-91 -0.3103555669476208 1102 92-93 -0.056724801577654915 1102 94-95 -0.46147544762027337 1102 96-97 -0.02888143418376643 1102 98-99 0.29045181557965094 1102 100-101 0.3587379013057763 1103 1 0.19719604509306876 1103 2 0.29899102268916167 1103 3 0.5058868222234096 1103 4 0.5451260606267567 1103 5 0.25526501505424903 1103 6 0.11769003541549239 1103 7 0.2942291370195491 1103 8 0.2414514564568222 1103 9 0.008394908453915662 1103 10-11 0.09239018208229055 1103 12-13 0.03646585992441942 1103 14-15 0.5541905738739885 1103 16-17 0.5724675966271988 1103 18-19 0.7003926958631226 1103 20-21 0.4223259778341486 1103 22-23 0.42010604353123426 1103 24-25 0.30558809019915856 1103 26-27 0.7034691523313441 1103 28-29 0.7782445906560795 1103 30-31 0.5727305394171083 1103 32-33 0.5029973334017583 1103 34-35 0.5675601490360833 1103 36-37 0.5200816850855787 1103 38-39 0.7119902343678675 1103 40-41 0.4546657152089253 1103 42-43 0.879972716133814 1103 44-45 1.1197617933570214 1103 46-47 0.7699129298784371 1103 48-49 0.9908711355730162 1103 50-51 0.9514953877205876 1103 52-53 1.0945055148952143 1103 54-55 1.0443563726344784 1103 56-57 1.7061704453969497 1103 58-59 1.4594910783072663 1103 60-61 1.4391074935804937 1103 62-63 1.4472579431735362 1103 64-65 1.526893410335557 1103 66-67 1.4337793485788595 1103 68-69 1.720227874272613 1103 70-71 1.8561167412727926 1103 72-73 1.4838449493333457 1103 74-75 1.5816338738362994 1103 76-77 1.9255808647974462 1103 78-79 2.2552372176520166 1103 80-81 2.454240249312871 1103 82-83 2.3886534640323838 1103 84-85 2.2446819944761174 1103 86-87 2.2990811314479522 1103 88-89 2.8643571331379505 1103 90-91 2.7066652909823254 1103 92-93 2.5407925800306366 1103 94-95 2.8608877951975167 1103 96-97 3.064946455098667 1103 98-99 2.7014120674489916 1103 100-101 2.620773845159899 1104 1 0.4856470576820122 1104 2 0.524292062645376 1104 3 0.5240176377680186 1104 4 0.5901452177148769 1104 5 0.02305373973952385 1104 6 0.05474531729835519 1104 7 0.2853347746769117 1104 8 0.34537865952195546 1104 9 0.47192802284909874 1104 10-11 0.6176706965869414 1104 12-13 0.5876426524805183 1104 14-15 0.5205630424016263 1104 16-17 0.4475702238849877 1104 18-19 0.04467293669508621 1104 20-21 0.4549614458144404 1104 22-23 0.10038792639932836 1104 24-25 0.2369304547311728 1104 26-27 0.16720752124212623 1104 28-29 0.12509051293303486 1104 30-31 0.5028413768555424 1104 32-33 0.5214360307197694 1104 34-35 0.6397755294411169 1104 36-37 0.9516293041331991 1104 38-39 0.6817836114888323 1104 40-41 0.4175146478854401 1104 42-43 0.6610345661611774 1104 44-45 1.0802160900182187 1104 46-47 1.0962676096813837 1104 48-49 1.2815115296616852 1104 50-51 1.332071468727694 1104 52-53 1.6754442122132254 1104 54-55 1.9863035538057936 1104 56-57 1.89483355431868 1104 58-59 2.035537876336818 1104 60-61 2.120689321714053 1104 62-63 2.376513553463635 1104 64-65 2.332414756805175 1104 66-67 2.3819867924759635 1104 68-69 1.8853893411582163 1104 70-71 2.265565290807551 1104 72-73 2.4278446756606584 1104 74-75 2.131305466611341 1104 76-77 2.133743152701115 1104 78-79 2.356427693842491 1104 80-81 2.377611896822451 1104 82-83 2.423033595395271 1104 84-85 2.4128880700097817 1104 86-87 2.676886276494475 1104 88-89 2.3856693936743483 1104 90-91 2.1646907425422626 1104 92-93 2.7188166632271304 1104 94-95 2.550440340353507 1104 96-97 1.6299779274577233 1104 98-99 1.4475122316526026 1104 100-101 1.2529919622918122 1105 1 -0.3408917757050318 1105 2 -0.2645010408029016 1105 3 -0.311907273608071 1105 4 0.040193558030701126 1105 5 -0.08659820998654766 1105 6 0.025418859032164676 1105 7 2.0747851191060818E-4 1105 8 -0.24167419255667966 1105 9 -0.18868267647413717 1105 10-11 -0.010209580564200849 1105 12-13 -0.19760549447404685 1105 14-15 -0.01647772459804031 1105 16-17 -0.15722755246047626 1105 18-19 -0.19255716320823524 1105 20-21 -0.0483015255036392 1105 22-23 -0.14546286315917456 1105 24-25 -0.2690113757554471 1105 26-27 0.10073958698495744 1105 28-29 -0.0439998834575519 1105 30-31 0.039519591486538275 1105 32-33 0.10826732301755015 1105 34-35 -0.03088592721373118 1105 36-37 0.23258724805842945 1105 38-39 0.5397235083628189 1105 40-41 0.005010619525791071 1105 42-43 0.714047779180838 1105 44-45 0.4303611109656913 1105 46-47 1.1465818217342374 1105 48-49 1.7897696669481888 1105 50-51 1.8732614461688861 1105 52-53 1.5233736353521259 1105 54-55 1.146974678523815 1105 56-57 1.2267905314376009 1105 58-59 0.8032828006036539 1105 60-61 0.9570573526518515 1105 62-63 0.44966856474303896 1105 64-65 0.48728504362438585 1105 66-67 0.35326458815755757 1105 68-69 0.5828595312967906 1105 70-71 0.38011169751073837 1105 72-73 0.7635883108524126 1105 74-75 0.8647891920383479 1105 76-77 0.7410385120307978 1105 78-79 1.1531566658051062 1105 80-81 1.1316805400709171 1105 82-83 1.3882084262041658 1105 84-85 1.6423232939865784 1105 86-87 1.1761208881606073 1105 88-89 1.2951722940932981 1105 90-91 1.1391308005506389 1105 92-93 0.999311345792389 1105 94-95 1.2845081779300465 1105 96-97 1.357076702836391 1105 98-99 1.301323059883348 1105 100-101 0.5321371443736673 1106 1 0.2647481140902208 1106 2 0.1834758378098158 1106 3 0.34759248329323356 1106 4 0.1624992141270809 1106 5 0.005563207548973992 1106 6 -0.004752390495141867 1106 7 0.16040952054097346 1106 8 0.4127745136180252 1106 9 0.4594454635507148 1106 10-11 0.4557836126722492 1106 12-13 0.4299537945973242 1106 14-15 0.4705705169880403 1106 16-17 0.6519129585870118 1106 18-19 0.17914074614296283 1106 20-21 -0.006245450737281999 1106 22-23 0.03548360110531945 1106 24-25 0.026583135616171205 1106 26-27 0.10531794579863885 1106 28-29 0.02780877085610456 1106 30-31 0.3100344205071224 1106 32-33 0.015120005206519238 1106 34-35 0.18737618720472682 1106 36-37 -0.057402487774318445 1106 38-39 0.22813850485139398 1106 40-41 0.11090711350234272 1106 42-43 -0.1034661314668881 1106 44-45 -0.24896469531165621 1106 46-47 0.26382292162079324 1106 48-49 0.307448344491263 1106 50-51 0.5830556226045154 1106 52-53 0.039431654908060665 1106 54-55 0.5057748513939977 1106 56-57 0.6267824281476635 1106 58-59 0.7035042905285707 1106 60-61 0.60703574387869 1106 62-63 0.8100893956003681 1106 64-65 0.4656492594960291 1106 66-67 0.6033591265401412 1106 68-69 0.8662045693822549 1106 70-71 0.4040569192707757 1106 72-73 0.7509336730694685 1106 74-75 0.48983646122963975 1106 76-77 0.8237595967912092 1106 78-79 0.7624970580043424 1106 80-81 0.5028112191266167 1106 82-83 0.3330520327084052 1106 84-85 -0.06965877073169935 1106 86-87 -0.26134872450213464 1106 88-89 0.37118115369029425 1106 90-91 0.20700935924546826 1106 92-93 0.5275918276680329 1106 94-95 0.7857776933533067 1106 96-97 0.8670842659069997 1106 98-99 1.1853784863865116 1106 100-101 1.616667963886389 1107 1 0.04680188295393961 1107 2 0.201941912217233 1107 3 0.18147540217440294 1107 4 0.18364209343809534 1107 5 0.1864997013224894 1107 6 0.15992930248697235 1107 7 0.10210172677133045 1107 8 -0.0674598037967229 1107 9 -0.10516639973826614 1107 10-11 0.002491918526310144 1107 12-13 0.08425801929375609 1107 14-15 0.44192094566476925 1107 16-17 0.22798852983269313 1107 18-19 -0.06557353402929067 1107 20-21 -0.24114477959633973 1107 22-23 0.24997714185870734 1107 24-25 -0.09026521756690187 1107 26-27 -0.12254022233575057 1107 28-29 -0.25238287285728944 1107 30-31 -0.2883672554055181 1107 32-33 -0.5578714212589375 1107 34-35 -0.2939637670180346 1107 36-37 0.14584071352917505 1107 38-39 -0.6975122690943678 1107 40-41 -0.5259590850467504 1107 42-43 -0.8118883683447251 1107 44-45 -0.9178167727450273 1107 46-47 -1.0468416242922345 1107 48-49 -0.7038795835065628 1107 50-51 -1.1544623197471928 1107 52-53 -0.43700082365809934 1107 54-55 -1.2303852913689326 1107 56-57 -0.8153753093702818 1107 58-59 -0.7063766110503593 1107 60-61 -0.41992627652710013 1107 62-63 -0.021368881157979303 1107 64-65 -0.19282534965165965 1107 66-67 0.12651930809734324 1107 68-69 -0.04061158455342451 1107 70-71 0.3694330297568662 1107 72-73 -0.19054516465408255 1107 74-75 -0.5388519043676006 1107 76-77 -0.8305446279052262 1107 78-79 -0.695585728976301 1107 80-81 -0.21643462018749915 1107 82-83 -0.3901896168241201 1107 84-85 -0.4093608307076444 1107 86-87 -0.06718338330649587 1107 88-89 -0.6389053575406471 1107 90-91 -0.6203561216095537 1107 92-93 -0.1338505434379904 1107 94-95 0.24060176132087818 1107 96-97 -0.2560293625214456 1107 98-99 0.43926761235151446 1107 100-101 0.30600341797336483 1108 1 0.02832479288848333 1108 2 -0.10400721364240795 1108 3 -0.35875162574837205 1108 4 -0.19252826036089488 1108 5 -0.10540517894928314 1108 6 -0.15215123442578005 1108 7 -0.05938638326264112 1108 8 0.09021688855558807 1108 9 -0.11241637902404022 1108 10-11 -0.5872835392580953 1108 12-13 -0.045765180639961045 1108 14-15 -0.5390633943799727 1108 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70-71 -0.2235959223814774 2316 72-73 -0.629873043946688 2316 74-75 -0.6135347045439552 2316 76-77 -0.6341746675858211 2316 78-79 -0.5797036930188213 2316 80-81 -0.04281976690772993 2316 82-83 -0.7884407313099864 2316 84-85 -1.2315084817143571 2316 86-87 -2.1891154853983004 2316 88-89 -1.8095074248506933 2316 90-91 -2.2522328668209397 2316 92-93 -2.4821335003771985 2316 94-95 -3.1036151592689443 2316 96-97 -2.890331033025536 2316 98-99 -2.059189253371308 2316 100-101 -2.68576653254836 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 416.0 3 115.0 4 106.0 5 113.0 6 135.0 7 127.0 8 177.0 9 280.0 10 357.0 11 613.0 12 955.0 13 1262.0 14 1566.0 15 2214.0 16 3044.0 17 4017.0 18 3483.0 19 1842.0 20 1814.0 21 1802.0 22 1858.0 23 1955.0 24 2215.0 25 2381.0 26 2466.0 27 2768.0 28 3214.0 29 3621.0 30 4348.0 31 5039.0 32 6181.0 33 8136.0 34 11160.0 35 19617.0 36 21370.0 37 23372.0 38 18703.0 39 3392.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 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14.725592699316012 32.520107561164885 36.2272981573834 16.527001582135703 44-45 14.994961875686164 33.06032213917254 34.773284405877305 17.17143157926398 46-47 15.31515815055885 32.48252463394973 33.19266816656039 19.009649048931024 48-49 16.2245729702741 32.44162648772374 32.95204694544787 18.381753596554287 50-51 17.715796502499504 33.07736730973994 32.48241925490125 16.724416932859302 52-53 15.928977281272466 33.47741211645416 32.92245312927687 17.67115747299651 54-55 14.898897224712274 32.78846327391302 33.684881811126424 18.627757690248288 56-57 16.52142786238761 34.36760207074338 30.670404673310454 18.440565393558554 58-59 15.800033087184731 32.79210720247853 32.095170774992106 19.31268893534463 60-61 16.370144225707968 31.803949287894966 32.924367978584215 18.90153850781285 62-63 18.98360655737705 31.58700556474658 32.22860580538427 17.200782072492103 64-65 18.011381751899467 32.03463854515704 33.52954623385529 16.424433469088196 66-67 16.595418239797837 32.30817827650006 33.303650667107895 17.792752816594216 68-69 16.3340475216062 33.75502539781892 32.79989889019957 17.11102819037531 70-71 16.68808441099197 33.23321666430921 31.378149085557617 18.700549839141203 72-73 15.88976486901908 32.649727931467005 31.229677578484775 20.230829621029137 74-75 16.226712512145745 33.03130668640068 32.17486259893328 18.567118202520298 76-77 16.98586040914561 31.563477737665462 32.47021660649819 18.98044524669073 78-79 15.671397067893341 31.433651534798084 33.73998537857751 19.154966018731066 80-81 15.586659442538078 33.50611040876528 32.23406176629944 18.673168382397208 82-83 15.37788073891329 32.615079126301225 31.522353932246222 20.48468620253926 84-85 15.627688438885581 30.982848135915436 31.5739286853045 21.815534739894478 86-87 16.423667314531222 31.24040989969131 31.986256446051737 20.34966633972573 88-89 17.39050628150154 29.459113819303397 32.25517189498232 20.895208004212744 90-91 15.339365290469727 29.830505414619324 33.43553759542157 21.39459169948938 92-93 15.072680935058058 31.602591298748585 32.64248081048796 20.6822469557054 94-95 15.033871181219844 31.637367794101724 31.916218460094576 21.41254256458386 96-97 14.605362509363184 30.950697763966563 30.971153875079345 23.472785851590913 98-99 15.469603285643363 30.588376886160884 31.260850596500728 22.681169231695026 100-101 16.694960148330082 30.37907245539262 31.459341664860702 21.4666257314166 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1895.0 1 1326.5 2 843.0 3 881.0 4 752.5 5 865.5 6 1522.0 7 2923.0 8 4270.5 9 4336.0 10 3218.5 11 2276.5 12 2172.0 13 2078.5 14 1790.5 15 1537.5 16 1311.0 17 1172.5 18 1041.0 19 949.0 20 935.0 21 956.5 22 994.0 23 1051.5 24 1125.5 25 1186.5 26 1289.0 27 1403.5 28 1498.0 29 1629.0 30 1848.0 31 2124.0 32 2509.0 33 2956.5 34 3290.5 35 3452.0 36 3740.0 37 3916.5 38 3816.0 39 3656.5 40 3423.0 41 3352.0 42 3204.0 43 3182.0 44 3515.0 45 3748.0 46 4047.0 47 5266.5 48 12768.0 49 12553.0 50 4924.0 51 3287.0 52 2397.0 53 2124.0 54 1929.0 55 1843.0 56 1738.0 57 1717.0 58 1703.5 59 1687.5 60 1786.0 61 1764.0 62 1495.5 63 931.5 64 497.0 65 391.0 66 323.5 67 240.0 68 177.5 69 130.0 70 111.5 71 88.0 72 52.0 73 63.5 74 66.0 75 44.0 76 28.0 77 18.0 78 13.0 79 6.0 80 3.0 81 1.5 82 1.5 83 1.5 84 1.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.039101037079814244 2 0.03188238408046391 3 0.027069948747563705 4 0.05594456074496499 5 0.10406891407396712 6 0.11670155682283019 7 0.06376476816092783 8 0.09023316249187902 9 0.07218652999350321 10-11 0.07489352486825958 12-13 0.073088861618422 14-15 0.06887798070213431 16-17 0.051733679828677305 18-19 0.060155441661252675 20-21 0.05624533795327125 22-23 0.032483938497076446 24-25 0.009925647874106693 26-27 0.003308549291368897 28-29 0.007820207415962848 30-31 0.007519430207656584 32-33 0.008421761832575375 34-35 0.0063163213744315315 36-37 0.0060155441661252675 38-39 0.0030077720830626337 40-41 0.005413989749512741 42-43 0.0030077720830626337 44-45 0.0021054404581438437 46-47 0.0012031088332250534 48-49 3.0077720830626336E-4 50-51 0.0018046632498375805 52-53 0.004511658124593951 54-55 0.011429533915638009 56-57 0.009324093457494166 58-59 0.005113212541206478 60-61 0.0021054404581438437 62-63 0.006617098582737794 64-65 0.0030077720830626337 66-67 0.020152072956519645 68-69 0.04812435332900214 70-71 0.05805000120310883 72-73 0.005714766957819004 74-75 0.015339637623619434 76-77 0.021655958998050964 78-79 0.02496450828941986 80-81 0.07579585649317837 82-83 0.02767150316417623 84-85 0.009023316249187901 86-87 0.02947616641401381 88-89 0.04421424962102072 90-91 0.0394018142881205 92-93 0.038800259871507974 94-95 0.010827979499025482 96-97 0.015941192040231957 98-99 0.035190933371832815 100-101 0.07218652999350321 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 166236.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.72759209798118 #Duplication Level Percentage of deduplicated Percentage of total 1 78.86533408537508 34.48591159556293 2 11.24485837311359 9.834211602781588 3 3.8532968317948577 5.0548617627950625 4 1.6893425596016014 2.9548352944007314 5 1.1115543877508909 2.430279843114608 6 0.6479481641468683 1.6999927813470008 7 0.4952470044434662 1.5159171298635674 8 0.3232862390117071 1.1309223032315503 9 0.246247816098279 0.9691041651627806 >10 1.3330398536269965 10.590365504463534 >50 0.08254116740724436 2.4423109314468587 >100 0.07841410903688215 6.617700137154407 >500 0.0151325473579948 4.448494910849635 >1k 0.012381175111086655 12.535792487788445 >5k 0.0013756861234540726 3.289299550037297 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5468 3.289299550037297 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3901 2.346663779205467 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3275 1.970090714406025 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2970 1.7866166173392046 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2510 1.5099015856974423 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2159 1.2987559854664452 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1875 1.1279145311484877 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1864 1.1212974325657499 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1145 0.6887798070213431 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1140 0.6857720349382805 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 964 0.5798984576144758 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 839 0.50470415553791 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 823 0.4950792848721095 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 786 0.472821771457446 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 665 0.4000336870473303 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 605 0.3639404220505787 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 582 0.3501046704684906 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 577 0.34709689838542795 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 544 0.3272456026372146 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 509 0.30619119805577616 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 501 0.3013787627228759 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 466 0.28032435814143747 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 444 0.2670901609759619 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 431 0.25926995355999904 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 429 0.258066844726774 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 392 0.2358093313121105 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 389 0.23400466806227294 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 349 0.20994249139777185 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 330 0.19851295748213385 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 319 0.19189585889939606 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 274 0.16482591015183232 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 270 0.16241969248538224 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 269 0.1618181380687697 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 237 0.14256839673716884 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 225 0.13534974373781852 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 216 0.12993575398830579 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 210 0.12632642748863063 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 202 0.1215139921557304 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 191 0.1148968935729926 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 181 0.10888134940686735 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 171 0.10286580524074208 No Hit GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG 170 0.10226425082412954 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0015038860415313169 0.0 24-25 0.0 0.0 0.0 0.004812435332900214 0.0 26-27 0.0 0.0 0.0 0.008120984624269112 0.0 28-29 0.0 0.0 0.0 0.018046632498375802 0.0 30-31 0.0 0.0 0.0 0.0427103635794894 0.0 32-33 0.0 0.0 0.0 0.057448446786496306 0.0 34-35 0.0 0.0 0.0 0.06647176303568421 0.0 36-37 0.0 0.0 0.0 0.08812772203373517 0.0 38-39 0.0 0.0 0.0 0.12121321494742415 0.0 40-41 0.0 0.0 0.0 0.163322024110301 0.0 42-43 0.0 0.0 0.0 0.21956736206357225 0.0 44-45 0.0 0.0 0.0 0.25475829543540507 0.0 46-47 0.0 0.0 0.0 0.33506581005317737 0.0 48-49 0.0 0.0 0.0 0.4406386101686759 0.0 50-51 0.0 0.0 0.0 0.4881614090810655 0.0 52-53 0.0 0.0 0.0 0.5621526023244063 0.0 54-55 0.0 0.0 0.0 0.6743425010226425 0.0 56-57 0.0 0.0 0.0 0.8151062345099738 0.0 58-59 0.0 0.0 0.0 0.977525926995356 0.0 60-61 0.0 0.0 0.0 1.1985971751004596 0.0 62-63 0.0 0.0 0.0 1.384176712625424 0.0 64-65 0.0 0.0 0.0 1.5487018455689503 0.0 66-67 0.0 0.0 0.0 1.8010539233379053 0.0 68-69 0.0 0.0 0.0 2.1063427897687625 0.0 70-71 0.0 0.0 0.0 2.2603407204215693 0.0 72-73 0.0 0.0 0.0 2.4877282899011046 0.0 74-75 0.0 0.0 0.0 2.7217329579633773 0.0 76-77 0.0 0.0 0.0 2.9181404749873674 0.0 78-79 0.0 0.0 0.0 3.14492649005029 0.0 80-81 0.0 0.0 0.0 3.5058591400178063 0.0 82-83 0.0 0.0 0.0 3.909201376356505 0.0 84-85 0.0 0.0 0.0 4.614223152626387 0.0 86-87 0.0 0.0 0.0 5.533097524002021 0.0 88-89 0.0 0.0 0.0 6.29466541543348 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCACT 20 1.7320986E-5 95.08072 1 GTCACTA 30 1.3553199E-6 79.23394 2 TCATGGG 25 0.0050028674 57.03125 9 GGGAGCT 35 2.7734655E-4 54.315483 7 ACTACCT 45 1.5027315E-5 52.822624 5 AGGGTAG 40 5.3523283E-4 47.54036 5 TCACTAC 50 2.8023687E-5 47.54036 3 CACTACC 55 4.920178E-5 43.218513 4 CTTTGCG 55 4.920178E-5 43.218513 2 GCTTTGC 55 4.920178E-5 43.218513 1 TAAGACA 45 9.561207E-4 42.258102 4 GAGTCAT 45 9.575397E-4 42.245377 6 TTGCGTT 60 8.2188795E-5 39.61697 4 TGGGAGT 120 8.54925E-11 39.60504 6 CTACCTC 65 1.3190354E-4 36.558495 6 TTTGCGT 70 2.0356175E-4 33.9574 3 TTGGTAT 30 0.003930565 31.693575 16-17 GAGGCGA 30 0.003953808 31.655434 46-47 TACCTCC 80 4.462933E-4 29.703777 7 TGGGGTA 65 0.005814277 29.246796 6 >>END_MODULE