##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936956_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 166236 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.003747684015494 31.0 31.0 34.0 30.0 34.0 2 32.07392502225751 33.0 31.0 34.0 30.0 34.0 3 32.108791116244376 33.0 31.0 34.0 30.0 34.0 4 35.87360740152554 37.0 35.0 37.0 35.0 37.0 5 35.73562284944296 37.0 35.0 37.0 35.0 37.0 6 35.92093168748045 37.0 35.0 37.0 35.0 37.0 7 35.87597151038283 37.0 35.0 37.0 35.0 37.0 8 35.87324646887557 37.0 35.0 37.0 35.0 37.0 9 37.465639211723094 39.0 37.0 39.0 35.0 39.0 10-11 37.394499386414495 39.0 37.0 39.0 34.0 39.0 12-13 37.239785606005924 39.0 37.0 39.0 33.5 39.0 14-15 38.03962739719435 40.0 37.0 41.0 33.0 41.0 16-17 38.06663719050025 39.5 37.0 41.0 33.5 41.0 18-19 38.36130561370581 40.0 37.5 41.0 34.0 41.0 20-21 38.4560143410573 40.0 38.0 41.0 34.0 41.0 22-23 38.342534108135425 40.0 37.5 41.0 34.0 41.0 24-25 38.170143651194685 40.0 37.0 41.0 34.0 41.0 26-27 37.76995355999904 40.0 36.0 41.0 33.0 41.0 28-29 37.48748766813446 39.0 35.5 41.0 33.0 41.0 30-31 37.087174258283405 38.5 35.0 40.5 33.0 41.0 32-33 36.569681055848314 38.0 35.0 40.0 32.5 41.0 34-35 36.274991578238165 38.0 35.0 40.0 31.5 41.0 36-37 35.9153462547222 38.0 35.0 40.0 31.0 41.0 38-39 35.529515267451096 37.5 35.0 40.0 30.5 41.0 40-41 34.94751738492264 37.0 35.0 40.0 28.0 41.0 42-43 34.545486537212156 37.0 34.0 40.0 25.5 41.0 44-45 34.01254541735845 37.0 33.5 40.0 23.5 41.0 46-47 33.13186975143772 36.0 33.0 40.0 17.0 41.0 48-49 32.36316441685315 36.0 33.0 40.0 4.5 41.0 50-51 31.950910151832332 35.5 33.0 40.0 2.0 41.0 52-53 31.584070237493684 35.0 32.0 40.0 2.0 41.0 54-55 31.19468707139248 35.0 31.0 39.0 2.0 41.0 56-57 30.97801017830073 35.0 31.0 39.0 2.0 41.0 58-59 30.678282682451453 35.0 31.0 39.0 2.0 40.0 60-61 30.221182535672177 35.0 29.5 38.5 2.0 40.0 62-63 29.719820014918547 35.0 28.0 38.0 2.0 40.0 64-65 29.288583700281528 35.0 28.5 37.5 2.0 40.0 66-67 28.976602540965857 35.0 28.0 37.0 2.0 39.5 68-69 28.53298924420703 34.0 27.0 36.5 2.0 39.0 70-71 28.074424312423304 34.0 26.0 36.0 2.0 39.0 72-73 27.62110192738035 34.0 26.0 35.0 2.0 38.0 74-75 27.118253567217693 33.5 25.5 35.0 2.0 37.0 76-77 25.649429124858635 31.5 22.5 34.5 2.0 36.0 78-79 26.209398084650736 33.0 23.5 35.0 2.0 36.0 80-81 26.06600555836281 33.0 22.5 35.0 2.0 36.0 82-83 25.68846098318054 33.0 20.0 35.0 2.0 35.5 84-85 25.238811087851005 33.0 17.5 35.0 2.0 35.0 86-87 24.76039185254698 33.0 9.0 35.0 2.0 35.0 88-89 24.23452200486056 32.0 2.0 35.0 2.0 35.0 90-91 23.613880266608916 31.0 2.0 34.0 2.0 35.0 92-93 23.115790201881662 31.0 2.0 34.0 2.0 35.0 94-95 22.529536321855673 31.0 2.0 34.0 2.0 35.0 96-97 21.674333477706394 30.0 2.0 34.0 2.0 35.0 98-99 20.859127986717677 29.5 2.0 34.0 2.0 35.0 100-101 18.225243027984313 23.5 2.0 31.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.010256604116815282 1101 2 0.2723096191041421 1101 3 0.39805679606676847 1101 4 0.18797786285514206 1101 5 -7.728163303610813E-4 1101 6 -0.09946754368692012 1101 7 0.08511196121209252 1101 8 0.1616572474614486 1101 9 0.32217032004756163 1101 10-11 -0.021741014293880312 1101 12-13 0.3808826499596165 1101 14-15 0.32204434100636803 1101 16-17 0.5559561065682246 1101 18-19 0.152909632229715 1101 20-21 -0.024230540988533278 1101 22-23 0.38592835276300974 1101 24-25 0.36397207140699095 1101 26-27 0.5030447089339631 1101 28-29 0.5118507800528889 1101 30-31 0.8928808800131023 1101 32-33 0.9581674347809539 1101 34-35 0.42833244659852454 1101 36-37 0.8452698979624742 1101 38-39 1.2353087809746697 1101 40-41 1.290365042134944 1101 42-43 1.8568277445315218 1101 44-45 1.5821851169520613 1101 46-47 1.9498641951900382 1101 48-49 2.3892817582378783 1101 50-51 2.479972414497748 1101 52-53 2.608639775809948 1101 54-55 2.498545734749282 1101 56-57 2.5382123325322254 1101 58-59 2.5641400349134464 1101 60-61 2.6378531328500436 1101 62-63 2.683947101903385 1101 64-65 2.552592556020304 1101 66-67 2.882413509986563 1101 68-69 2.7605569754518022 1101 70-71 2.946831768905774 1101 72-73 2.855296447794494 1101 74-75 2.835913961727396 1101 76-77 2.9688630960973157 1101 78-79 3.082172040870841 1101 80-81 2.8771036580083837 1101 82-83 3.4587690876194763 1101 84-85 2.8321570034567927 1101 86-87 2.490776879528415 1101 88-89 2.460497259232401 1101 90-91 3.1422439019414483 1101 92-93 3.32337580085494 1101 94-95 3.6252897842246874 1101 96-97 3.094850515440182 1101 98-99 2.928060947123754 1101 100-101 2.847094428774053 1102 1 -0.2333649425417441 1102 2 -0.1306070609614629 1102 3 -0.08150714900832057 1102 4 -0.1022752162200149 1102 5 -0.13091398750442806 1102 6 -0.005294472329552491 1102 7 0.028016421449414963 1102 8 -0.04908655419144736 1102 9 0.1409631296433389 1102 10-11 0.12394828049339424 1102 12-13 0.12900947891417758 1102 14-15 0.1329023015813462 1102 16-17 -0.22555014805440265 1102 18-19 -0.06291504539191806 1102 20-21 0.10976952332634937 1102 22-23 -0.14295707026814597 1102 24-25 0.018568977860994096 1102 26-27 -0.0037485508660211053 1102 28-29 0.0010539186193128103 1102 30-31 0.1011083617637567 1102 32-33 0.06937591148288647 1102 34-35 0.27389632735872027 1102 36-37 0.33327517178309307 1102 38-39 0.7787052500874481 1102 40-41 0.8420098953362114 1102 42-43 0.33950292220139744 1102 44-45 0.39453035925838265 1102 46-47 0.17253840813662435 1102 48-49 0.6612050948941501 1102 50-51 0.7417259598558168 1102 52-53 1.3576107376390567 1102 54-55 1.1898182047624637 1102 56-57 0.7978836834664023 1102 58-59 0.37610742726640467 1102 60-61 1.0998430354129916 1102 62-63 0.6567017084570743 1102 64-65 0.7402392743532609 1102 66-67 0.7863994250265982 1102 68-69 0.6751628859897956 1102 70-71 0.6423490213738567 1102 72-73 0.5140175462927381 1102 74-75 0.6419322271549284 1102 76-77 1.2123351352007674 1102 78-79 0.3639596731183232 1102 80-81 0.9644030759586677 1102 82-83 0.5458353641091698 1102 84-85 0.039090148133126945 1102 86-87 0.2190143300863454 1102 88-89 -0.01348736950992091 1102 90-91 0.08401481231865304 1102 92-93 0.2581444280537042 1102 94-95 0.20639889442824355 1102 96-97 1.0624116949751858 1102 98-99 0.6988426945591968 1102 100-101 0.6023039345141541 1103 1 0.21462141453552164 1103 2 0.29412729559107476 1103 3 0.26835510994484935 1103 4 0.22072753860092575 1103 5 0.22740950718270625 1103 6 0.13041325428726935 1103 7 0.1713327011293302 1103 8 0.014831850636049637 1103 9 0.09882092841023393 1103 10-11 0.2266145536142119 1103 12-13 0.057531714254594135 1103 14-15 0.2866311484920061 1103 16-17 0.32497130019431353 1103 18-19 0.3483731613508354 1103 20-21 0.1972350880646374 1103 22-23 0.34559730464986416 1103 24-25 0.1773483298221663 1103 26-27 0.2773251733895208 1103 28-29 0.05072268419716863 1103 30-31 0.29617592046767527 1103 32-33 0.4401944868469272 1103 34-35 0.3068065989053608 1103 36-37 0.34870544857701447 1103 38-39 0.5494647935742663 1103 40-41 0.2738280771543984 1103 42-43 -0.03826829647891117 1103 44-45 0.31642790615593697 1103 46-47 0.3319900675882792 1103 48-49 0.18598208558022833 1103 50-51 0.2998723589113048 1103 52-53 0.4224638138557708 1103 54-55 0.8686355994889539 1103 56-57 0.6464669184132745 1103 58-59 0.8346375335237859 1103 60-61 0.7506858794376541 1103 62-63 1.4477124653769238 1103 64-65 1.3474477224708004 1103 66-67 1.0977105886104894 1103 68-69 1.0354705076610564 1103 70-71 1.1932804132143389 1103 72-73 1.6600722491656228 1103 74-75 1.1706446763219205 1103 76-77 1.1440221350695836 1103 78-79 1.0625855421987396 1103 80-81 1.3825619372993465 1103 82-83 1.5243379221571836 1103 84-85 1.4976038566468333 1103 86-87 1.3641015660826703 1103 88-89 1.5653857775450426 1103 90-91 1.321355754205051 1103 92-93 1.0511721074450193 1103 94-95 1.6750628062739743 1103 96-97 1.4892285649738746 1103 98-99 1.227702723419224 1103 100-101 1.789238212357521 1104 1 0.25416711787433144 1104 2 0.09338837933491817 1104 3 0.2324355697149585 1104 4 0.06842868802620927 1104 5 -0.4382308869059628 1104 6 0.1447126522073532 1104 7 0.0620004624867434 1104 8 0.019415868151106963 1104 9 -0.0029168931551737387 1104 10-11 0.21501767348284773 1104 12-13 0.4221663612168314 1104 14-15 0.1986795885576882 1104 16-17 0.07517655470991969 1104 18-19 0.1476684541806108 1104 20-21 0.6095914099037216 1104 22-23 0.1992166259416024 1104 24-25 0.3504120955583474 1104 26-27 0.447412748649505 1104 28-29 0.12382750083646954 1104 30-31 -0.09784433131119386 1104 32-33 0.5933143554840328 1104 34-35 0.660562756540827 1104 36-37 0.43720435388626555 1104 38-39 0.3660698838862473 1104 40-41 1.051845318533715 1104 42-43 1.2843233838713886 1104 44-45 1.360831299697253 1104 46-47 0.956237467697747 1104 48-49 0.750671467079961 1104 50-51 0.7583083195024436 1104 52-53 0.8841496376105624 1104 54-55 0.8662751725595612 1104 56-57 1.0248536179206624 1104 58-59 1.225031622193736 1104 60-61 1.4359964979379285 1104 62-63 1.614310713871724 1104 64-65 1.5418921669152432 1104 66-67 1.1702338507889252 1104 68-69 1.0941390900365349 1104 70-71 1.0999580267885065 1104 72-73 1.333820196620465 1104 74-75 1.7443652565080185 1104 76-77 1.424468221550157 1104 78-79 1.4736419187723584 1104 80-81 1.3787305196748214 1104 82-83 1.3364274131259855 1104 84-85 1.2845838785406514 1104 86-87 1.410557504779991 1104 88-89 1.2995469707579588 1104 90-91 1.8887134444075677 1104 92-93 1.435545396990726 1104 94-95 2.100350162595813 1104 96-97 2.031989922390405 1104 98-99 1.2968050770043362 1104 100-101 2.4789960120291177 1105 1 -0.06093117410117799 1105 2 -0.116086322566467 1105 3 -0.33133336357540344 1105 4 -0.133633832115585 1105 5 0.10724929356454993 1105 6 -0.2873289204642546 1105 7 -0.08471239603726133 1105 8 0.007451639984815017 1105 9 -0.23877735077683582 1105 10-11 -0.27811397331057464 1105 12-13 0.10019971603474431 1105 14-15 -0.021168139447468093 1105 16-17 -0.8061479699436447 1105 18-19 -0.6676111139792411 1105 20-21 -0.08002347891355299 1105 22-23 -0.10615317747444664 1105 24-25 -0.06477079196984903 1105 26-27 -0.1482607930842974 1105 28-29 0.09476288594764526 1105 30-31 -0.4887966355329141 1105 32-33 -0.39347262485757284 1105 34-35 -0.32620408200251205 1105 36-37 -0.161994002543004 1105 38-39 0.17240649361876592 1105 40-41 0.5121458341637393 1105 42-43 0.481350454448247 1105 44-45 0.5763485410765696 1105 46-47 -0.039653605457267815 1105 48-49 -0.8415175435549109 1105 50-51 -1.0976576811420955 1105 52-53 -1.0665029566530606 1105 54-55 -0.6030488686908413 1105 56-57 -1.137912253816367 1105 58-59 -0.8708030539261493 1105 60-61 -1.567608883950566 1105 62-63 -1.137864600340329 1105 64-65 -1.3786685807483074 1105 66-67 -1.0484657947154261 1105 68-69 -0.6801317768664639 1105 70-71 -0.03773130611513409 1105 72-73 -0.036245062805896566 1105 74-75 -0.6447944276685824 1105 76-77 -0.7114889167031464 1105 78-79 -1.0797338466159765 1105 80-81 -0.5596758612468626 1105 82-83 -0.42487213569773274 1105 84-85 -0.7283270141438507 1105 86-87 -1.0411424629931325 1105 88-89 -1.0889681758743315 1105 90-91 -1.1840588727049024 1105 92-93 -1.151950574649625 1105 94-95 -0.8486910878070262 1105 96-97 -0.21591533059057966 1105 98-99 -0.17201541928957198 1105 100-101 -0.12153170308529226 1106 1 -0.14642088292296052 1106 2 -0.14868855907448975 1106 3 -0.03607648928444007 1106 4 -0.0071144652526413665 1106 5 -0.6144567190765855 1106 6 -0.03574877892678785 1106 7 -0.21879483350687678 1106 8 0.20211967520711482 1106 9 0.2610605834244524 1106 10-11 0.16402853654842886 1106 12-13 0.321122410648762 1106 14-15 0.43461490846998885 1106 16-17 0.08429555012550338 1106 18-19 0.010796319438732382 1106 20-21 0.26997111889316017 1106 22-23 0.12612762853279236 1106 24-25 -0.0723878845094248 1106 26-27 0.007632999795610829 1106 28-29 0.20010565511192624 1106 30-31 0.1922443671141636 1106 32-33 0.14897510892233612 1106 34-35 -0.18896136145797726 1106 36-37 -0.044644858790640285 1106 38-39 -0.01838546791761786 1106 40-41 -0.1091198997242131 1106 42-43 -0.40316415848459997 1106 44-45 -0.19821793291187362 1106 46-47 -0.8821121436237576 1106 48-49 -0.7412933525333543 1106 50-51 -0.7244876741192421 1106 52-53 -0.8491322041275318 1106 54-55 -0.9210354712514821 1106 56-57 -1.017240761190358 1106 58-59 -1.0122864962037141 1106 60-61 -0.7888016882291033 1106 62-63 -0.624278084214783 1106 64-65 -0.6820962723911173 1106 66-67 -1.0448598306940369 1106 68-69 -0.7823682848887223 1106 70-71 -1.114064297309561 1106 72-73 -0.7377738835070993 1106 74-75 -0.9634157879408534 1106 76-77 -0.23660911550386032 1106 78-79 -0.5884718942633214 1106 80-81 -1.1766063350192155 1106 82-83 -0.9112180921560586 1106 84-85 -1.1797748019456975 1106 86-87 -0.9062902129796235 1106 88-89 -0.7686586301987894 1106 90-91 -0.4832593480979135 1106 92-93 -0.19570834030248108 1106 94-95 -0.4277791975795928 1106 96-97 -0.18238121573995514 1106 98-99 -0.40625452036460885 1106 100-101 0.12085120437513908 1107 1 0.0693140745973082 1107 2 0.11363832147794284 1107 3 0.2974378839940641 1107 4 0.03001165493201796 1107 5 0.2384353940475208 1107 6 0.20178856056190142 1107 7 0.14629807877927448 1107 8 0.01400624075004231 1107 9 0.3642145578978173 1107 10-11 0.05375581280245001 1107 12-13 0.19723289674101352 1107 14-15 0.3759967605086203 1107 16-17 0.5982614887529607 1107 18-19 -0.009861631447719788 1107 20-21 -0.16797743989956615 1107 22-23 -0.5431317887772167 1107 24-25 -0.19730544667724814 1107 26-27 -0.11330814929078059 1107 28-29 -0.041252920362325085 1107 30-31 -0.0063435213135107915 1107 32-33 0.4237355542806043 1107 34-35 0.48477879449500705 1107 36-37 0.01440002618434022 1107 38-39 -0.008221368138741525 1107 40-41 -0.21778266110063527 1107 42-43 -0.4934190368645517 1107 44-45 -0.16190649248048317 1107 46-47 0.36320344779495173 1107 48-49 0.9537060655525309 1107 50-51 0.36842461202731513 1107 52-53 0.3241230234008867 1107 54-55 0.47621789415178384 1107 56-57 -0.19701342674492395 1107 58-59 -0.6065177876650942 1107 60-61 -1.0153602481856545 1107 62-63 -0.8984178211896037 1107 64-65 -0.033611188789453905 1107 66-67 -0.5262773734647226 1107 68-69 -0.8111924304448372 1107 70-71 -0.7097503740446385 1107 72-73 -0.43224957889446003 1107 74-75 -0.1794804967898287 1107 76-77 -0.2745887097157578 1107 78-79 0.059935600790410604 1107 80-81 -0.38524194040386206 1107 82-83 -0.4761015153627888 1107 84-85 -0.2412637261804811 1107 86-87 0.27722610001257664 1107 88-89 0.403646137617482 1107 90-91 0.5411810445001421 1107 92-93 0.6457976534128491 1107 94-95 0.696595244752718 1107 96-97 0.4235659464589041 1107 98-99 0.3690684419661494 1107 100-101 1.0999604878448999 1108 1 0.03119989778067378 1108 2 -0.008782757318975598 1108 3 -0.15757081598107803 1108 4 -0.1527292515225369 1108 5 -0.1702977238578569 1108 6 -0.03360790973389527 1108 7 -0.020907157777195096 1108 8 0.04437329684416369 1108 9 0.11601669560599959 1108 10-11 0.10866922733555384 1108 12-13 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-0.44614735189070487 2316 100-101 -1.9843975193074055 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 3.0 5 11.0 6 58.0 7 83.0 8 140.0 9 280.0 10 396.0 11 681.0 12 1048.0 13 1324.0 14 1741.0 15 2495.0 16 3473.0 17 4560.0 18 3870.0 19 2223.0 20 1871.0 21 1929.0 22 1935.0 23 2178.0 24 2433.0 25 2595.0 26 2953.0 27 3352.0 28 3700.0 29 4442.0 30 5179.0 31 6139.0 32 7590.0 33 9550.0 34 13696.0 35 19390.0 36 20155.0 37 22597.0 38 11640.0 39 526.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.08881976740688 19.438912720424998 17.29948920962861 14.172778302539513 2 15.069152424094762 25.89831976754679 39.69270936731097 19.33981844104748 3 20.42590308900051 33.0110987457515 33.039371973411136 13.523626191836858 4 10.683606439038476 19.997473471450228 38.33104742655021 30.98787266296109 5 9.829093249754859 42.29656321624727 31.983805668016196 15.890537865981678 6 22.52099424913978 39.828316369498786 18.738420077480207 18.912269303881228 7 21.199980750258668 42.113020043793156 20.63151182655983 16.05548737938834 8 25.64907721552492 30.667845713323228 20.773478668880387 22.90959840227147 9 28.5822565509276 18.37929209076253 22.21420149666739 30.824249861642482 10-11 23.061190115257826 29.261712264491447 25.10617435453211 22.57092326571862 12-13 30.067107230194946 28.850393890264186 26.45125777177526 14.631241107765607 14-15 25.553002183734485 27.78757015923816 32.07081796798393 14.588609689043427 16-17 15.944801366731634 31.741921124184895 32.66530715368513 19.64797035539835 18-19 15.427858152615274 28.475952717538423 40.517039131350195 15.579149998496106 20-21 18.793518833221544 26.004674091875685 39.705417916149074 15.496389158753695 22-23 17.307831129612474 26.776411564421238 39.31325721573204 16.602500090234248 24-25 17.303620198034096 27.13343840610224 40.74467317155335 14.81826822431031 26-27 18.04307744134075 25.86631615990568 41.74559585648072 14.34501054227286 28-29 15.846747996823792 26.916251594119206 42.37409466060299 14.862905748454006 30-31 15.6118409971366 29.583844654587455 40.6310907384682 14.173223609807742 32-33 15.317680887413076 29.576626001588103 39.98081041411006 15.124882696888761 34-35 16.155044635337713 28.018299285353354 40.08036767005944 15.746288409249502 36-37 17.200306799211948 28.124163445776247 40.21686493315086 14.458664821860948 38-39 18.0510475732098 27.25404966384406 40.347737151623626 14.347165611322515 40-41 15.821277107708967 28.705447108009746 40.76698649502211 14.70628928925918 42-43 15.187745133424771 30.248562284944295 38.99456194807382 15.569130633557112 44-45 15.743976900171445 30.72367431647968 37.37329683881253 16.159051944536348 46-47 16.220012512331866 30.304807622897567 35.6366851945427 17.83849467022787 48-49 17.07121201183859 30.303303736856037 35.25108881349407 17.374395437811305 50-51 18.302348174728024 31.141964261544615 34.64663083396747 15.909056729759888 52-53 16.859214788658257 31.588018203091394 35.16697696941989 16.385790038830457 54-55 15.393476743906254 31.006821627084385 35.637888303375924 17.961813325633436 56-57 17.26676913174382 32.531559143504246 32.848579274583344 17.353092450168585 58-59 16.846832214442117 31.050434322288794 33.62087634447412 18.481857118794967 60-61 16.963641176187497 30.378326675970936 34.45395591703162 18.204076230809953 62-63 19.630525277316586 29.95921461055367 34.040761327269664 16.369498784860077 64-65 18.557352198079837 30.67446281190597 34.857672225029475 15.910512764984722 66-67 17.028200871050796 30.741836906566565 35.05438051926178 17.175581703120866 68-69 17.119688634497447 32.32943625158284 34.201178448646644 16.34969666527306 70-71 17.60995695865203 31.86383173269248 32.6374330392726 17.888778269382893 72-73 16.8559062294155 31.234603920341925 32.4768175269422 19.43267232330038 74-75 17.144825179873425 31.94167047669466 32.90570782299011 18.0077965204418 76-77 17.783505929840498 30.44536215188693 33.756628397664755 18.014503520607814 78-79 16.49983457154029 30.26348241946642 34.66778957499925 18.568893433994045 80-81 16.216590166695745 32.58928839987728 33.12589107928124 18.068230354145737 82-83 16.0847111477392 31.717653569785032 32.491254876365154 19.706380406110608 84-85 16.318255920977435 30.136677274395268 32.268650251758096 21.2764165528692 86-87 16.933257135982196 30.18828766505249 32.759045928956 20.119409270009324 88-89 17.843489350694664 28.718985270541385 33.188354794368095 20.249170584395856 90-91 16.00895134510804 28.89936955580153 34.45606140815246 20.635617690937966 92-93 15.653047474674558 30.474145191173996 33.50567867369282 20.367128660458626 94-95 15.45573762602565 31.044719555330975 32.48965326403426 21.00988955460911 96-97 15.23591531291451 30.038108586914348 31.70742711394377 23.01854898622737 98-99 16.078743946822268 29.845700363942612 31.58510542304569 22.49045026618943 100-101 16.99472420908254 29.80551160734159 31.67519897010786 21.524565213468005 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2838.0 1 2011.0 2 1234.0 3 1186.5 4 914.5 5 926.0 6 1591.0 7 3064.5 8 4285.5 9 4316.5 10 3220.5 11 2104.0 12 1881.0 13 1765.0 14 1559.0 15 1365.5 16 1218.5 17 1113.5 18 1010.0 19 941.0 20 933.0 21 971.5 22 1019.0 23 1075.5 24 1118.5 25 1191.5 26 1320.0 27 1415.0 28 1485.5 29 1639.0 30 1854.0 31 2055.0 32 2414.0 33 2795.5 34 3109.5 35 3329.5 36 3623.0 37 3830.0 38 3823.0 39 3698.0 40 3437.0 41 3332.0 42 3172.0 43 3267.5 44 3645.5 45 3834.0 46 3998.5 47 5258.0 48 12896.0 49 12508.5 50 4808.5 51 3216.0 52 2184.5 53 1907.0 54 1818.0 55 1722.5 56 1705.0 57 1696.0 58 1748.5 59 1877.5 60 1905.5 61 1782.5 62 1486.5 63 944.5 64 517.5 65 381.0 66 308.0 67 252.0 68 190.0 69 131.5 70 92.0 71 76.5 72 65.5 73 65.0 74 59.5 75 39.0 76 26.0 77 16.5 78 11.5 79 10.0 80 5.5 81 3.0 82 1.5 83 2.0 84 3.0 85 1.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.013835751582088116 2 0.005413989749512741 3 6.015544166125267E-4 4 0.0 5 0.0030077720830626337 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005714766957819004 14-15 0.004210880916287687 16-17 0.0 18-19 6.015544166125267E-4 20-21 3.0077720830626336E-4 22-23 0.0012031088332250534 24-25 0.0012031088332250534 26-27 3.0077720830626336E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0021054404581438437 38-39 0.011128756707331745 40-41 6.015544166125267E-4 42-43 0.0 44-45 6.015544166125267E-4 46-47 0.0 48-49 0.0 50-51 3.0077720830626336E-4 52-53 3.0077720830626336E-4 54-55 0.0 56-57 3.0077720830626336E-4 58-59 0.0 60-61 0.002406217666450107 62-63 0.0 64-65 0.0 66-67 0.0 68-69 3.0077720830626336E-4 70-71 3.0077720830626336E-4 72-73 3.0077720830626336E-4 74-75 0.004812435332900214 76-77 3.0077720830626336E-4 78-79 6.015544166125267E-4 80-81 0.0030077720830626337 82-83 3.0077720830626336E-4 84-85 0.0030077720830626337 86-87 6.015544166125267E-4 88-89 0.0027069948747563705 90-91 0.002406217666450107 92-93 0.0 94-95 0.0 96-97 3.0077720830626336E-4 98-99 6.015544166125267E-4 100-101 0.0030077720830626337 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 166236.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.89788012223586 #Duplication Level Percentage of deduplicated Percentage of total 1 78.30026328562813 32.023147813951255 2 11.411005045082149 9.333718328159964 3 3.943400944298175 4.8383021728145525 4 1.837115919219851 3.0053658653961834 5 1.079618162295733 2.2077047089679733 6 0.6663038522070396 1.6350249043528478 7 0.4780325650492006 1.3685362977934985 8 0.3441834468354244 1.12610986789865 9 0.24857693382558432 0.9149642676676532 >10 1.4796946475061408 11.125748935248684 >50 0.10443172959536383 2.943405760485094 >100 0.07354347154603086 5.869366442888424 >500 0.020592172032888642 6.427007387088237 >1k 0.011766955447364938 13.783416347842826 >5k 0.0014708694309206172 3.3981808994441636 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5649 3.3981808994441636 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4496 2.7045886570899205 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3652 2.196876729468948 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3424 2.0597223224812917 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3295 1.9821218027382757 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2895 1.7415000360932649 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2353 1.4154575422892754 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1524 0.9167689309174908 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1274 0.7663803267643591 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 913 0.549219182367237 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 900 0.5413989749512741 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 900 0.5413989749512741 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 867 0.5215476792030607 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 858 0.516133689453548 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 847 0.5095165908708101 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 837 0.503501046704685 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 779 0.4686108905411584 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 721 0.4337207343776318 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 668 0.40183835029716786 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 666 0.4006352414639428 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 632 0.38018239129911696 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 552 0.3320580379701148 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 544 0.3272456026372146 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 489 0.2941601097235256 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 453 0.2725041507254746 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 375 0.22558290622969754 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 369 0.22197357973002238 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 345 0.20753627373132172 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 341 0.2051300560648716 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 305 0.18347409706682066 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 264 0.15881036598570708 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 261 0.1570057027358695 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 259 0.15580259390264442 No Hit GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG 245 0.14738083207006908 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 240 0.14437305998700642 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 238 0.14316995115378137 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 238 0.14316995115378137 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 231 0.13895907023749368 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 230 0.13835751582088116 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 229 0.13775596140426863 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 228 0.1371544069876561 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 220 0.1323419716547559 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 205 0.12331865540556798 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 197 0.11850622007266777 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 179 0.10767824057364228 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0015038860415313169 0.0 24-25 0.0 0.0 0.0 0.004812435332900214 0.0 26-27 0.0 0.0 0.0 0.008120984624269112 0.0 28-29 0.0 0.0 0.0 0.018046632498375802 0.0 30-31 0.0 0.0 0.0 0.04331191799610193 0.0 32-33 0.0 0.0 0.0 0.05865155561972136 0.0 34-35 0.0 0.0 0.0 0.06857720349382805 0.0 36-37 0.0 0.0 0.0 0.09053393970018528 0.0 38-39 0.0 0.0 0.0 0.12422098703048678 0.0 40-41 0.0 0.0 0.0 0.16663057340166992 0.0 42-43 0.0 0.0 0.0 0.22197357973002238 0.0 44-45 0.0 0.0 0.0 0.25746529031016147 0.0 46-47 0.0 0.0 0.0 0.33807358213624006 0.0 48-49 0.0 0.0 0.0 0.44635337712649487 0.0 50-51 0.0 0.0 0.0 0.49387617603888445 0.0 52-53 0.0 0.0 0.0 0.5687697009071441 0.0 54-55 0.0 0.0 0.0 0.6815611540219928 0.0 56-57 0.0 0.0 0.0 0.825633436800693 0.0 58-59 0.0 0.0 0.0 0.9913616785774441 0.0 60-61 0.0 0.0 0.0 1.2148391443489979 0.0 62-63 0.0 0.0 0.0 1.4040280083736376 0.0 64-65 0.0 0.0 0.0 1.572463245025145 0.0 66-67 0.0 0.0 0.0 1.8308308669602251 0.0 68-69 0.0 0.0 0.0 2.1370220650160014 0.0 70-71 0.0 0.0 0.0 2.291019995668808 0.0 72-73 0.0 0.0 0.0 2.5172044563151186 0.0 74-75 0.0 0.0 0.0 2.7512091243773913 0.0 76-77 0.0 0.0 0.0 2.9536321855675065 0.0 78-79 0.0 0.0 0.0 3.187336076421473 0.0 80-81 0.0 0.0 0.0 3.5554873793883397 0.0 82-83 0.0 0.0 0.0 3.9591303929353447 0.0 84-85 0.0 0.0 0.0 4.664753723621839 0.0 86-87 0.0 0.0 0.0 5.581522654539329 0.0 88-89 0.0 0.0 0.0 6.345496763637239 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCACT 25 5.254877E-5 76.02094 1 CTGGTCG 45 1.5105399E-5 52.77644 9 GTCACTA 45 9.602488E-4 42.221153 2 GTATATG 50 0.0016120418 37.99904 8 CCTGGTC 50 0.0016120418 37.99904 8 AGTATAT 55 0.002573628 34.54458 7 GAATAAC 60 0.003936002 31.675396 1 CATGAGA 60 0.003941807 31.665865 6 TATAGGA 100 4.928855E-5 28.499277 2 TGGTCGG 50 4.9435206E-5 28.499277 10-11 TGGGAGT 135 4.1894782E-7 28.147432 6 ACTACCT 85 6.3849124E-4 27.940468 5 TATATGG 70 0.008372327 27.14217 9 TACCTGG 70 0.008372327 27.14217 2 TCACTAC 70 0.008372327 27.14217 3 ATAACCC 70 0.008372327 27.14217 3 TAACCCT 125 7.342709E-6 26.599327 4 GAGTACT 2945 0.0 25.967083 12-13 CATGGGT 110 9.430522E-5 25.908434 4 CCGGGCG 65 9.995105E-6 25.576275 58-59 >>END_MODULE