FastQCFastQC Report
Thu 26 May 2016
SRR936955_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936955_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2727832
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136780.5014238413509337No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99880.36615158118241886No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65420.23982415339361074No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74900.042.640831
GTATCAA132200.040.444481
ATCAACG165100.032.2098963
TCAACGC166300.031.9488954
TATCAAC168400.031.6374682
CAACGCA169900.031.2970335
AACGCAG174050.030.6042776
ACGCAGA195000.026.9502667
CGCAGAG196200.026.7859238
GTACATG132100.025.8321061
GCAGAGT207500.024.7322799
TACATGG133550.024.5551952
ACATGGG135050.023.2249973
GAGTACT108050.023.10652712-13
AGTACTT115150.021.47551212-13
AGAGTAC182100.021.23875610-11
GTACTTT119350.020.91938814-15
CATGGGG61100.020.7671244
CAGAGTA202100.019.94803610-11
CATGGGA77300.018.8125764
Produced by FastQC (version 0.11.5)