##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936955_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2727832 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.485165875317836 31.0 31.0 34.0 30.0 34.0 2 31.583192073412146 33.0 31.0 34.0 30.0 34.0 3 31.582876071546927 33.0 31.0 34.0 28.0 34.0 4 35.17446858897469 37.0 35.0 37.0 33.0 37.0 5 35.198034189788814 37.0 35.0 37.0 33.0 37.0 6 35.24701227934858 37.0 35.0 37.0 32.0 37.0 7 35.171959270218984 37.0 35.0 37.0 32.0 37.0 8 35.14215281586256 37.0 35.0 37.0 32.0 37.0 9 36.7131194296423 39.0 37.0 39.0 32.0 39.0 10-11 36.569407133577144 39.0 37.0 39.0 32.0 39.0 12-13 36.43660148425563 39.0 37.0 39.0 32.0 39.0 14-15 37.52072231721015 40.0 37.0 41.0 32.0 41.0 16-17 37.447578516565535 40.0 37.0 41.0 32.0 41.0 18-19 37.40806856873884 40.0 37.0 41.0 32.0 41.0 20-21 37.348975303464435 40.0 37.0 41.0 31.5 41.0 22-23 37.198237098179064 40.0 37.0 41.0 31.0 41.0 24-25 37.00875585446611 39.0 36.0 41.0 31.0 41.0 26-27 36.96568850281102 39.0 36.0 41.0 30.5 41.0 28-29 37.031938917059406 39.0 36.0 41.0 31.0 41.0 30-31 36.878864607497825 39.0 36.0 41.0 30.0 41.0 32-33 36.683519366295286 39.0 36.0 41.0 30.0 41.0 34-35 36.47317870015455 39.0 35.0 41.0 30.0 41.0 36-37 36.30334841001938 39.0 35.0 41.0 30.0 41.0 38-39 36.13076886699767 39.0 35.0 41.0 29.5 41.0 40-41 35.91278018587655 39.0 35.0 40.0 28.5 41.0 42-43 35.68122230401286 38.5 35.0 40.0 27.5 41.0 44-45 35.41290519357497 38.0 35.0 40.0 26.5 41.0 46-47 35.02636196070726 38.0 34.0 40.0 25.0 41.0 48-49 34.65878782124412 38.0 34.0 40.0 24.0 41.0 50-51 33.99610918121057 37.5 33.5 39.5 23.0 40.5 52-53 34.29011225764636 38.0 33.5 40.0 24.0 40.5 54-55 34.58574648292124 38.0 34.0 40.0 23.5 41.0 56-57 34.458064682869036 38.0 34.0 40.0 23.0 41.0 58-59 34.208047636364704 38.0 34.0 40.0 22.5 41.0 60-61 33.91406765519284 38.0 33.5 40.0 22.0 41.0 62-63 33.590199103170576 37.0 33.0 40.0 21.5 41.0 64-65 33.27187451426627 37.0 33.0 40.0 20.0 41.0 66-67 32.899937752764835 36.0 32.0 39.0 19.5 41.0 68-69 32.46025543361908 36.0 32.0 39.0 18.0 41.0 70-71 32.098723088518646 35.0 31.5 38.5 16.0 40.0 72-73 31.721099759809256 35.0 31.0 38.0 13.0 40.0 74-75 31.29135701905396 35.0 31.0 37.0 9.5 39.0 76-77 30.853237296138474 35.0 31.0 36.5 7.5 39.0 78-79 30.42225236011602 35.0 30.0 36.0 6.5 39.0 80-81 29.998795197064922 34.0 30.0 36.0 2.0 37.5 82-83 29.589816198358257 34.0 29.0 35.0 2.0 37.0 84-85 29.236652770405215 34.0 29.0 35.0 2.0 36.5 86-87 28.901606110640245 34.0 29.0 35.0 2.0 36.0 88-89 28.519417068206547 34.0 29.0 35.0 2.0 36.0 90-91 28.16595505148411 34.0 28.5 35.0 2.0 35.5 92-93 27.79165469134463 34.0 27.0 35.0 2.0 35.0 94-95 27.366542184416048 34.0 26.5 35.0 2.0 35.0 96-97 26.90171920411521 33.5 25.5 35.0 2.0 35.0 98-99 26.269850196053127 33.0 25.0 35.0 2.0 35.0 100-101 24.082029978385766 31.0 12.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.668338049533137 1101 2 0.5494481854487496 1101 3 0.6794176239002354 1101 4 0.5428945502805789 1101 5 0.5196451117608873 1101 6 0.4600030717539525 1101 7 0.47993298253247474 1101 8 0.3977900048691225 1101 9 0.5186307754374937 1101 10-11 0.6230363172437592 1101 12-13 0.7022031136371751 1101 14-15 0.9489289918057864 1101 16-17 0.9730940913198012 1101 18-19 1.0954524651266269 1101 20-21 0.9698145949013011 1101 22-23 1.0760391391428357 1101 24-25 1.2044540201117826 1101 26-27 1.2065916108956571 1101 28-29 1.0695361459514956 1101 30-31 0.979765424998817 1101 32-33 1.0083267955655941 1101 34-35 1.072723868037798 1101 36-37 1.0138032575343416 1101 38-39 1.1060689541444901 1101 40-41 1.0861934833158813 1101 42-43 1.024833525468928 1101 44-45 1.0333968407843415 1101 46-47 1.0808844388397105 1101 48-49 1.043002767021349 1101 50-51 0.9882824626992601 1101 52-53 1.1605694246507952 1101 54-55 1.1175234052560867 1101 56-57 1.1115131594116576 1101 58-59 1.0942174289202669 1101 60-61 1.2191375991117113 1101 62-63 1.2243940052074862 1101 64-65 1.12513051571797 1101 66-67 1.1003166395941548 1101 68-69 1.0117114514915357 1101 70-71 1.0357222503320216 1101 72-73 1.0822249684080525 1101 74-75 0.989769744703775 1101 76-77 0.9689193483897256 1101 78-79 1.1090132998400044 1101 80-81 1.1203324379901183 1101 82-83 1.0015749096032778 1101 84-85 1.264365665839673 1101 86-87 1.427079644301049 1101 88-89 1.382102454572692 1101 90-91 1.273153144087491 1101 92-93 1.1954869766545606 1101 94-95 1.32426588005778 1101 96-97 1.2842625027766275 1101 98-99 1.4634997230361932 1101 100-101 1.7312534611505903 1102 1 0.5132409331757408 1102 2 0.5363925129444205 1102 3 0.5251991943093373 1102 4 0.41944220149534317 1102 5 0.3520924272104793 1102 6 0.46377754983076613 1102 7 0.3920831573272565 1102 8 0.33819640011688534 1102 9 0.39405519476063944 1102 10-11 0.5663628535841099 1102 12-13 0.6495274653045868 1102 14-15 0.7300961894547982 1102 16-17 0.5804745365071327 1102 18-19 0.5918047106478426 1102 20-21 0.559048430311428 1102 22-23 0.5520079986513977 1102 24-25 0.6609608579495614 1102 26-27 0.6189682648480712 1102 28-29 0.8163979041209757 1102 30-31 0.7916392690387255 1102 32-33 0.775964154520139 1102 34-35 0.6572115905110252 1102 36-37 0.6098186382183073 1102 38-39 0.7218382920608164 1102 40-41 0.6176293140466669 1102 42-43 0.7049355117827858 1102 44-45 0.6103099481935956 1102 46-47 0.612489147092667 1102 48-49 0.5797651780791853 1102 50-51 0.4639030941388569 1102 52-53 0.6379178433288146 1102 54-55 0.629291681207711 1102 56-57 0.7418368596569422 1102 58-59 0.6810105259402732 1102 60-61 0.6973780576626112 1102 62-63 0.8646872394241143 1102 64-65 0.830390539973493 1102 66-67 0.7828931398025674 1102 68-69 0.6561279155051736 1102 70-71 0.8612487292801987 1102 72-73 0.809955914310688 1102 74-75 0.920350348243236 1102 76-77 0.7476413566131477 1102 78-79 0.8905038521244855 1102 80-81 0.8337747745638033 1102 82-83 0.9566762675356522 1102 84-85 1.0335349664720361 1102 86-87 0.980404358335111 1102 88-89 0.9835868487186907 1102 90-91 0.8302078258436936 1102 92-93 0.9470990369708012 1102 94-95 1.0506837704973755 1102 96-97 0.8937066574673906 1102 98-99 1.032630064016928 1102 100-101 1.3851317792666151 1103 1 0.4750979960606081 1103 2 0.5421437103071121 1103 3 0.5244891115824544 1103 4 0.41692332359523476 1103 5 0.44630677068252567 1103 6 0.33390058273491974 1103 7 0.40131983083599465 1103 8 0.4397509189947826 1103 9 0.5572459641827976 1103 10-11 0.5535681779605497 1103 12-13 0.43315855319411156 1103 14-15 0.582073113010189 1103 16-17 0.8340327252239348 1103 18-19 0.7634716261171448 1103 20-21 0.6306579967728325 1103 22-23 0.5107194497927807 1103 24-25 0.7626155198889819 1103 26-27 0.7897396152243843 1103 28-29 0.8487005103286052 1103 30-31 0.9868001821444068 1103 32-33 0.9919811975007917 1103 34-35 0.8892187435868522 1103 36-37 0.948836614208858 1103 38-39 0.9171277739293302 1103 40-41 1.0718506861932084 1103 42-43 1.0641314438596652 1103 44-45 1.1267637642040427 1103 46-47 1.1407319784666754 1103 48-49 1.3040388609802278 1103 50-51 1.1256680378472623 1103 52-53 1.139244769994228 1103 54-55 1.1683374608929782 1103 56-57 1.2748830747468247 1103 58-59 1.2911973767208949 1103 60-61 1.1820475233589534 1103 62-63 1.4272726098290462 1103 64-65 1.3595011949464393 1103 66-67 1.3151682533187738 1103 68-69 1.2914953970004746 1103 70-71 1.2341195385107753 1103 72-73 1.2459027327469663 1103 74-75 1.3056185574862589 1103 76-77 1.2504430609112127 1103 78-79 1.2351888679856522 1103 80-81 1.2828988648791615 1103 82-83 1.336617114708634 1103 84-85 1.4656994872159537 1103 86-87 1.383569936490236 1103 88-89 1.4101720325216398 1103 90-91 1.4165366185288981 1103 92-93 1.3694872312856639 1103 94-95 1.4730768164636636 1103 96-97 1.3245061847423791 1103 98-99 1.4517184511538304 1103 100-101 1.701505418627196 1104 1 0.3936573461433497 1104 2 0.4674435448189236 1104 3 0.4277049984487107 1104 4 0.4332097686080232 1104 5 0.38755485252395516 1104 6 0.3779282643958197 1104 7 0.4540612179279151 1104 8 0.44502969137329984 1104 9 0.4918709077663692 1104 10-11 0.47423915734974287 1104 12-13 0.574194810152143 1104 14-15 0.6500679979206794 1104 16-17 0.5986739167389032 1104 18-19 0.5542789075975136 1104 20-21 0.6154388001427762 1104 22-23 0.6747567598574733 1104 24-25 0.6456797593955343 1104 26-27 0.638456931306834 1104 28-29 0.5447071298562136 1104 30-31 0.5970496179801188 1104 32-33 0.6458301519163214 1104 34-35 0.736699712444981 1104 36-37 0.7011817323072478 1104 38-39 0.6903963161288118 1104 40-41 0.6461380338688585 1104 42-43 0.7036683778148287 1104 44-45 0.5378126584420428 1104 46-47 0.5531646548620444 1104 48-49 0.4625121572040811 1104 50-51 0.6392501049452051 1104 52-53 0.7939780331204602 1104 54-55 0.8373755231767177 1104 56-57 0.820719692769984 1104 58-59 0.9699225158814144 1104 60-61 0.8310446649265728 1104 62-63 0.708315185427054 1104 64-65 0.7536140278033656 1104 66-67 0.7208803038678866 1104 68-69 0.8141961642639046 1104 70-71 0.7407189066467765 1104 72-73 0.8186179426160827 1104 74-75 0.6206776819032882 1104 76-77 0.7293511397437662 1104 78-79 0.8754196487890233 1104 80-81 0.9610547246655337 1104 82-83 0.9363727939624304 1104 84-85 0.9062254387730455 1104 86-87 0.8295458654807568 1104 88-89 0.8896800812654355 1104 90-91 0.9646428318582174 1104 92-93 0.9641675381910346 1104 94-95 0.7960769669074708 1104 96-97 0.6363003776113452 1104 98-99 0.726192048265311 1104 100-101 0.8054614890348972 1105 1 0.3400179857698937 1105 2 0.41170553781660857 1105 3 0.37037406746859247 1105 4 0.4009786513090319 1105 5 0.3336390850335249 1105 6 0.37398690365381526 1105 7 0.41285608289792464 1105 8 0.30441012993926364 1105 9 0.4185493132044229 1105 10-11 0.39005783428101637 1105 12-13 0.3570084298568972 1105 14-15 0.40201717996036024 1105 16-17 0.4912058059303632 1105 18-19 0.5021915219993431 1105 20-21 0.5756392464890823 1105 22-23 0.5587149097725046 1105 24-25 0.5678803695386492 1105 26-27 0.5553136705218336 1105 28-29 0.5414393834955646 1105 30-31 0.3580905467080271 1105 32-33 0.40107164983814414 1105 34-35 0.25528348427602054 1105 36-37 0.3364093075002188 1105 38-39 0.31082792072331955 1105 40-41 0.4644628019939958 1105 42-43 0.4438733285734102 1105 44-45 0.37809541268540414 1105 46-47 0.4871791099305298 1105 48-49 0.6184958594214862 1105 50-51 0.3735737264926726 1105 52-53 0.430001046572265 1105 54-55 0.4514784285226412 1105 56-57 0.5087748103965737 1105 58-59 0.6187288466281231 1105 60-61 0.4539536962731958 1105 62-63 0.4716646254467136 1105 64-65 0.6942622536791703 1105 66-67 0.742212790917705 1105 68-69 0.711272391204389 1105 70-71 0.6965828324468148 1105 72-73 0.7709336200182655 1105 74-75 0.7797561201007035 1105 76-77 0.5768245739486346 1105 78-79 0.6005900998444922 1105 80-81 0.7112144265553368 1105 82-83 0.6567798290354254 1105 84-85 0.7846636361891548 1105 86-87 0.8494768660745216 1105 88-89 0.7794130924650382 1105 90-91 0.7590809064267141 1105 92-93 0.7653509701436967 1105 94-95 0.733839297736079 1105 96-97 0.8116121034154808 1105 98-99 0.9334226536992674 1105 100-101 1.2533822932772338 1106 1 0.5143992103183415 1106 2 0.4702408509259932 1106 3 0.44169396861340005 1106 4 0.39449637490741907 1106 5 0.47890528223755524 1106 6 0.5419099834399717 1106 7 0.6195446643651792 1106 8 0.4549142562450825 1106 9 0.506109262263486 1106 10-11 0.6870581897114363 1106 12-13 0.7308600808086894 1106 14-15 0.9514769516314203 1106 16-17 0.9230965716790891 1106 18-19 0.8986474976408445 1106 20-21 0.8914930927383651 1106 22-23 0.9646732296596028 1106 24-25 0.9316997345384124 1106 26-27 1.0531849736262657 1106 28-29 0.9479735224711803 1106 30-31 0.9856141265235578 1106 32-33 1.0385992938580202 1106 34-35 1.0331059351262155 1106 36-37 0.906694820240979 1106 38-39 1.0204117535611275 1106 40-41 0.973108813886121 1106 42-43 1.0648161285443436 1106 44-45 1.18873321191262 1106 46-47 0.8311589271544975 1106 48-49 0.8537952260409369 1106 50-51 0.9347240503815328 1106 52-53 0.9420994507619511 1106 54-55 0.8936425921111564 1106 56-57 1.0505681511275498 1106 58-59 1.1275326685344496 1106 60-61 1.08236714733075 1106 62-63 1.1694034363076966 1106 64-65 1.107535664198302 1106 66-67 1.1583848612310277 1106 68-69 1.0741463958253874 1106 70-71 1.0552256881386626 1106 72-73 1.070662623072078 1106 74-75 1.0217376330835926 1106 76-77 1.1134465861756055 1106 78-79 1.1521242381384162 1106 80-81 1.2275318456860553 1106 82-83 1.2816715604587294 1106 84-85 1.3467507993977001 1106 86-87 1.3346659020209835 1106 88-89 1.4307595765874446 1106 90-91 1.273523692559447 1106 92-93 1.3696674114658443 1106 94-95 1.375923663310509 1106 96-97 1.2409981549734788 1106 98-99 1.470215905129546 1106 100-101 1.6757725553291145 1107 1 0.2542191692018463 1107 2 0.279004528418767 1107 3 0.3489896261180867 1107 4 0.40539476686768694 1107 5 0.2660703415598036 1107 6 0.33495975433255154 1107 7 0.4378517320451536 1107 8 0.44037427891245784 1107 9 0.47693775733947064 1107 10-11 0.5014505422517743 1107 12-13 0.5143787744444381 1107 14-15 0.458567388801292 1107 16-17 0.504976254938363 1107 18-19 0.468265747547548 1107 20-21 0.5395380456537211 1107 22-23 0.6843240291599031 1107 24-25 0.5965524892741172 1107 26-27 0.6494878786666334 1107 28-29 0.5042722237322579 1107 30-31 0.7494932616401542 1107 32-33 0.6893156456613241 1107 34-35 0.7633651932909942 1107 36-37 0.7859067840056113 1107 38-39 0.8700103954883716 1107 40-41 0.931070007006177 1107 42-43 0.8520812123186303 1107 44-45 0.9331554544535905 1107 46-47 1.011032660226384 1107 48-49 1.0007617835426146 1107 50-51 0.7800412691310328 1107 52-53 0.7901537615043921 1107 54-55 0.9731347981710812 1107 56-57 0.893879665948262 1107 58-59 0.8438310916681573 1107 60-61 0.8843182977350708 1107 62-63 0.8166111099576767 1107 64-65 0.8472817454995649 1107 66-67 0.8680938386030519 1107 68-69 0.9081376017856613 1107 70-71 0.856226497605185 1107 72-73 0.8986855343934828 1107 74-75 1.0217287967453252 1107 76-77 0.9492575708160196 1107 78-79 1.028442083833351 1107 80-81 0.9406566472305897 1107 82-83 1.0240244339779885 1107 84-85 1.106909546387552 1107 86-87 1.0757212099659235 1107 88-89 1.0628820273762827 1107 90-91 0.9169407219505565 1107 92-93 1.0194734031405623 1107 94-95 1.0310786816553268 1107 96-97 1.2316543066873251 1107 98-99 1.1398814946320925 1107 100-101 1.345130901004353 1108 1 -0.05059988174886598 1108 2 0.1635143256483218 1108 3 0.12452070933801451 1108 4 0.19347101802487998 1108 5 0.15320649169440514 1108 6 0.07794672548906334 1108 7 0.08039496954638281 1108 8 -0.043710289117811385 1108 9 -0.11200841655762162 1108 10-11 -0.08058592379174456 1108 12-13 -0.08959013806948235 1108 14-15 0.09795806959462539 1108 16-17 0.14069897393087416 1108 18-19 0.12316310914275164 1108 20-21 0.23466740086674776 1108 22-23 0.09606310673143525 1108 24-25 0.2949005266285809 1108 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3026.0 5 3134.0 6 3483.0 7 4027.0 8 4993.0 9 6038.0 10 7534.0 11 9433.0 12 11785.0 13 14329.0 14 15903.0 15 18609.0 16 22896.0 17 29266.0 18 25759.0 19 18217.0 20 17500.0 21 18356.0 22 20324.0 23 22286.0 24 25462.0 25 29209.0 26 34210.0 27 39953.0 28 47184.0 29 56631.0 30 67918.0 31 83804.0 32 104846.0 33 135151.0 34 180575.0 35 250805.0 36 364031.0 37 508727.0 38 433847.0 39 77458.0 40 50.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.91286422421224 18.857715695487688 14.404788366833268 22.824631713466808 2 15.211576339496697 20.385550196818613 40.532232166707274 23.870641296977418 3 18.865338712061824 25.223482940682974 29.979637181603984 25.93154116565122 4 12.509795788774348 16.756038518784973 37.60276172420565 33.131403968235034 5 13.577094208416282 37.53632468831133 33.95345082245443 14.933130280817958 6 31.12118355450183 36.38225754427192 17.784875458094554 14.711683443131692 7 27.822337961263955 33.296108780346025 21.040631156180183 17.840922102209838 8 26.234376502171198 34.46522888777501 20.035542058591922 19.26485255146186 9 26.724290749231745 16.49039462012111 20.61760851126613 36.16770611938102 10-11 24.6627973694259 26.8323182406765 27.407997155183946 21.096887234713655 12-13 25.831725162264906 24.28100122385503 28.015712896929614 21.871560716950455 14-15 22.795272260738603 25.229940929861616 26.6971633968054 25.277623412594373 16-17 21.80164886188676 28.7676692279788 27.045687283587107 22.384994626547332 18-19 21.77198252005532 26.912572942409046 29.926452661296075 21.388991876239555 20-21 22.627692641809073 26.03113612710073 29.957140776572587 21.384030454517607 22-23 22.283232954526692 26.06648737031984 29.89062825014372 21.75965142500975 24-25 22.07603679874403 26.25358560329839 29.871853656031515 21.798523941926064 26-27 21.807597958306374 26.522742377760274 29.795636239328278 21.87402342460508 28-29 21.660185983382547 26.41690357843767 30.032244456264557 21.890665981915227 30-31 21.830996376143837 26.22844065988255 29.953910036454957 21.98665292751866 32-33 21.646146894379413 26.514537446872815 29.797896625534204 22.041419033213568 34-35 22.10434298810594 26.419182623426458 29.621671718415538 21.854802670052063 36-37 22.098935347898525 26.260128682521678 29.516674903790697 22.1242610657891 38-39 21.78190476819239 26.602399757632845 29.627844641477797 21.987850832696974 40-41 22.108574221669542 26.31403933997467 29.69668290777235 21.880703530583443 42-43 21.902990556263603 26.48090371996023 29.333557332674054 22.282548391102118 44-45 22.003174344111002 26.669441849078368 29.138773485516023 22.188610321294604 46-47 22.143030680864914 26.87343054517784 28.68278103498068 22.30075773897657 48-49 22.26588165266633 27.04586801327118 28.351457763076848 22.336792570985644 50-51 22.214034791791953 27.144674890358917 28.261418806929022 22.379871510920104 52-53 22.40954717937415 27.190013176844403 28.060957483131034 22.339482160650416 54-55 22.15102826278548 27.135736262414323 28.147146489624948 22.566088985175252 56-57 22.168770591088606 27.506347534020858 27.977215814322825 22.347666060567718 58-59 22.32556697941523 27.114234745369338 28.085547084403235 22.4746511908122 60-61 22.394705655592524 27.052950413020042 28.380314416803813 22.17202951458362 62-63 22.29320320045308 27.07027887136821 28.387221369806536 22.249296558372176 64-65 22.14085697138019 27.092904854366793 28.295700656885597 22.47053751736741 66-67 22.07623755856989 27.46374109610221 27.929383976918142 22.53063736840976 68-69 22.017085910221855 27.287555359203726 28.25190448131482 22.443454249259602 70-71 22.07428093899344 27.268637063172203 28.11111522613675 22.545966771697604 72-73 22.18897176143048 27.265108961106126 27.966448628954744 22.57947064850865 74-75 22.051253843488208 27.521209087592112 27.826139570189383 22.601397498730297 76-77 22.162927277058227 27.359998210271463 27.875040273476387 22.60203423919392 78-79 22.170093785334565 27.44107351974242 27.791143076969632 22.597689617953375 80-81 22.058301740617306 27.677584681467494 27.660494817572122 22.603618760343082 82-83 22.175238177682587 27.57377336613576 27.75077662449229 22.50021183168936 84-85 22.139328849803714 27.493721860251508 27.51468567483675 22.852263615108033 86-87 22.137884257515125 27.385825523858713 27.699188831977473 22.777101386648688 88-89 22.18131398307782 27.37803829899102 27.603154897868315 22.83749282006284 90-91 22.06186841430023 27.42116143264917 27.817988503459766 22.698981649590838 92-93 22.177964834789915 27.23503424164411 27.79487847357565 22.792122449990323 94-95 22.04993332794013 27.105636462728494 27.761919201424835 23.082511007906543 96-97 22.004221363843588 27.155275925108857 27.838234255422783 23.00226845562477 98-99 21.82374623662702 27.148641442081583 27.70545364724657 23.32215867404483 100-101 21.846531582945833 27.131862606570188 27.760695555155923 23.260910255328053 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 672.0 1 520.5 2 460.5 3 590.5 4 588.5 5 573.5 6 902.0 7 1953.0 8 3304.0 9 3972.5 10 3644.0 11 3232.5 12 3392.5 13 3648.5 14 3916.5 15 4192.0 16 4400.5 17 4695.0 18 4850.5 19 5065.5 20 5759.5 21 6684.5 22 7817.0 23 9486.0 24 11212.5 25 13520.5 26 17173.0 27 21215.5 28 24591.0 29 28177.0 30 33083.0 31 38270.5 32 43303.5 33 48609.0 34 55353.0 35 60136.5 36 65738.0 37 72927.5 38 75990.0 39 76917.0 40 78479.0 41 82217.0 42 85634.5 43 88952.0 44 92638.0 45 95111.5 46 95715.0 47 97560.0 48 103971.0 49 105392.5 50 100930.0 51 96722.0 52 93042.5 53 92047.5 54 89780.5 55 82998.5 56 75636.0 57 68331.0 58 61781.0 59 55890.0 60 48851.5 61 40210.0 62 31982.5 63 25078.5 64 18962.0 65 14379.0 66 10419.5 67 8005.5 68 5744.0 69 3423.5 70 2211.0 71 1501.5 72 1119.5 73 847.5 74 609.5 75 495.0 76 353.0 77 199.5 78 121.0 79 69.5 80 41.0 81 30.0 82 21.0 83 19.5 84 17.0 85 15.0 86 13.5 87 13.0 88 11.0 89 9.0 90 6.5 91 3.0 92 4.5 93 3.0 94 1.5 95 1.5 96 2.0 97 2.0 98 1.0 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.057114954293372905 2 0.09091468976095302 3 0.06543658113842789 4 0.12636408693790527 5 0.10106927406086591 6 0.0984298153258705 7 0.10733798855648002 8 0.09568037914358363 9 0.11587957029611795 10-11 0.10541338322887919 12-13 0.0889900844333522 14-15 0.09210611210661067 16-17 0.10490015514151897 18-19 0.08462764569079034 20-21 0.07078881690661301 22-23 0.07298836585244253 24-25 0.060560914308505805 26-27 0.05381563087462864 28-29 0.06532660369113641 30-31 0.048261769786409135 32-33 0.04970980617574689 34-35 0.044669173174887604 36-37 0.04965481745210115 38-39 0.050956217245050284 40-41 0.0632187026180498 42-43 0.05988272005020837 44-45 0.04708867701530006 46-47 0.035541045049695144 48-49 0.04294619316732115 50-51 0.027567680121063174 52-53 0.039555221875834 54-55 0.04556732232776799 56-57 0.050571296179530115 58-59 0.05574023620222947 60-61 0.05319242533997695 62-63 0.05839802451177345 64-65 0.04038005273052006 66-67 0.06263215623249525 68-69 0.05255089023077668 70-71 0.025624745218913773 72-73 0.0202175207270829 74-75 0.029950524812378473 76-77 0.04257960167634957 78-79 0.060835857926734495 80-81 0.039206959959410995 82-83 0.0590578891955223 84-85 0.06254050835975236 86-87 0.06463007985829039 88-89 0.08635062569835679 90-91 0.0625954970833981 92-93 0.07275008138331099 94-95 0.07982529715906259 96-97 0.08833021974960335 98-99 0.11785916434736449 100-101 0.11371668049938559 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2727832.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.049952017621614 #Duplication Level Percentage of deduplicated Percentage of total 1 73.37564748778136 37.45823283513156 2 14.379433659059737 14.681387966711453 3 5.106241915700678 7.820202143606636 4 2.3232334495941087 4.744038245100511 5 1.318825233639449 3.3662982448461243 6 0.7974194235943541 2.4424933987446757 7 0.4953564275250726 1.770154529974279 8 0.35288569426239147 1.441183820784014 9 0.2709392968131632 1.2448294291800108 >10 1.403612244650557 13.006383254886284 >50 0.11149910618963141 3.9402626136454932 >100 0.06115664772718308 5.639157035385659 >500 0.003172580621963457 1.0587404604945085 >1k 3.605205252159479E-4 0.27470639559661875 >5k 1.4420821008637917E-4 0.6084546052810006 >10k+ 7.210410504318959E-5 0.5034750206311873 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13678 0.5014238413509337 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9988 0.36615158118241886 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6542 0.23982415339361074 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 3.665914909715848E-5 0.0 7 0.0 0.0 0.0 3.665914909715848E-5 0.0 8 0.0 0.0 0.0 3.665914909715848E-5 0.0 9 0.0 0.0 0.0 3.665914909715848E-5 0.0 10-11 0.0 0.0 0.0 3.665914909715848E-5 3.665914909715848E-5 12-13 0.0 0.0 0.0 3.665914909715848E-5 9.164787274289619E-5 14-15 0.0 0.0 0.0 1.0997744729147543E-4 1.0997744729147543E-4 16-17 0.0 0.0 0.0 1.466365963886339E-4 1.0997744729147543E-4 18-19 0.0 0.0 0.0 1.466365963886339E-4 1.0997744729147543E-4 20-21 0.0 0.0 0.0 1.6496617093721315E-4 1.8329574548579239E-4 22-23 0.0 0.0 0.0 2.932731927772678E-4 1.8329574548579239E-4 24-25 0.0 0.0 0.0 6.048759601031148E-4 1.8329574548579239E-4 26-27 0.0 0.0 0.0 9.16478727428962E-4 1.8329574548579239E-4 28-29 0.0 0.0 0.0 0.0016496617093721315 1.8329574548579239E-4 30-31 0.0 0.0 0.0 0.0032626642696471043 1.8329574548579239E-4 32-33 0.0 0.0 0.0 0.005590520237316668 1.8329574548579239E-4 34-35 0.0 0.0 0.0 0.008486593015992187 1.8329574548579239E-4 36-37 0.0 0.0 0.0 0.010686141961821695 1.8329574548579239E-4 38-39 0.0 0.0 0.0 0.013600544315045794 1.8329574548579239E-4 40-41 0.0 0.0 0.0 0.017596391566636067 1.8329574548579239E-4 42-43 0.0 0.0 0.0 0.022600365418398202 1.8329574548579239E-4 44-45 0.0 0.0 0.0 0.02773264629200039 1.8329574548579239E-4 46-47 0.0 0.0 0.0 0.03411133823490596 1.8329574548579239E-4 48-49 0.0 0.0 0.0 0.039646869748576896 2.0162532003437162E-4 50-51 0.0 0.0 0.0 0.0486466908519293 2.1995489458295086E-4 52-53 0.0 0.0 0.0 0.07417978819810017 2.1995489458295086E-4 54-55 0.0 0.0 0.0 0.10847442217849193 2.1995489458295086E-4 56-57 0.0 0.0 0.0 0.13466738420841168 2.382844691315301E-4 58-59 0.0 0.0 0.0 0.17174811352018748 2.932731927772678E-4 60-61 0.0 0.0 0.0 0.21768202733892705 2.932731927772678E-4 62-63 0.0 0.0 0.0 0.24469982022353282 2.932731927772678E-4 64-65 0.0 0.0 0.0 0.26761178840925687 2.932731927772678E-4 66-67 0.0 0.0 0.0 0.337044216799275 2.932731927772678E-4 68-69 0.0 0.0 0.0 0.41030752626994627 2.932731927772678E-4 70-71 0.0 0.0 0.0 0.44042301725326194 2.932731927772678E-4 72-73 0.0 0.0 0.0 0.5040999592350262 3.116027673258471E-4 74-75 0.0 0.0 0.0 0.5973425049636487 3.299323418744263E-4 76-77 0.0 0.0 0.0 0.6854344402441206 3.299323418744263E-4 78-79 0.0 0.0 0.0 0.7705019957240768 3.6659149097158477E-4 80-81 7.331829819431695E-5 0.0 0.0 0.8884894670932815 3.6659149097158477E-4 82-83 7.331829819431695E-5 0.0 0.0 1.0255946847166542 4.0325064006874325E-4 84-85 7.331829819431695E-5 0.0 0.0 1.1527469433601483 4.0325064006874325E-4 86-87 7.331829819431695E-5 0.0 0.0 1.3727384970921963 4.2158021461732246E-4 88-89 7.331829819431695E-5 0.0 0.0 1.6492767883066113 4.3990978916590173E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7490 0.0 42.64083 1 GTATCAA 13220 0.0 40.44448 1 ATCAACG 16510 0.0 32.209896 3 TCAACGC 16630 0.0 31.948895 4 TATCAAC 16840 0.0 31.637468 2 CAACGCA 16990 0.0 31.297033 5 AACGCAG 17405 0.0 30.604277 6 ACGCAGA 19500 0.0 26.950266 7 CGCAGAG 19620 0.0 26.785923 8 GTACATG 13210 0.0 25.832106 1 GCAGAGT 20750 0.0 24.732279 9 TACATGG 13355 0.0 24.555195 2 ACATGGG 13505 0.0 23.224997 3 GAGTACT 10805 0.0 23.106527 12-13 AGTACTT 11515 0.0 21.475512 12-13 AGAGTAC 18210 0.0 21.238756 10-11 GTACTTT 11935 0.0 20.919388 14-15 CATGGGG 6110 0.0 20.767124 4 CAGAGTA 20210 0.0 19.948036 10-11 CATGGGA 7730 0.0 18.812576 4 >>END_MODULE