FastQCFastQC Report
Thu 26 May 2016
SRR936955_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936955_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2727832
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140150.513777974596676No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113660.41666788863830323No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76290.279672648462222No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28330.10385536939224996No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84650.044.447241
GTATCAA143050.037.1611181
ATCAACG181650.028.9459063
TATCAAC184850.028.6503752
TCAACGC183850.028.6248424
CAACGCA186450.028.20025
AACGCAG192050.027.4526066
ACGCAGA215200.024.3007667
GTACATG124800.024.1716181
CGCAGAG217450.024.0488828
TACATGG127750.022.8659422
GAGTACT128500.022.60249112-13
GCAGAGT230750.022.1069859
ACATGGG127600.021.4783083
AGTACTT133600.021.33083212-13
GTACTTT139150.020.89022614-15
AGAGTAC198400.020.76800710-11
GTATAGG8150.019.2354741
CAGAGTA224250.019.05178610-11
TACTTTT153450.018.40178114-15
Produced by FastQC (version 0.11.5)