FastQCFastQC Report
Thu 26 May 2016
SRR936954_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936954_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2637544
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134270.5090720761435639No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96060.36420245501117704No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65040.24659304261843593No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72000.041.775821
GTATCAA129450.041.0019871
ATCAACG162750.032.32193
TCAACGC164950.031.8620034
TATCAAC167500.031.518192
CAACGCA167450.031.3331185
AACGCAG172200.030.439496
ACGCAGA192550.026.9766547
CGCAGAG191800.026.8100978
GTACATG121950.025.8725991
TACATGG121950.025.6003322
GCAGAGT202800.024.699749
ACATGGG125000.023.721653
GAGTACT109050.023.22387912-13
AGTACTT114000.022.52807212-13
AGAGTAC176600.021.99865710-11
TAGCGCG1100.002871556721.5996954
GTACTTT117950.021.32929414-15
CATGGGG53700.020.8837264
GTGTAGC10850.019.707731
Produced by FastQC (version 0.11.5)