FastQCFastQC Report
Thu 26 May 2016
SRR936954_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936954_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2637544
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136130.5161240911999951No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109020.41333907604953696No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71880.27252625927757035No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27720.10509777277649206No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA81850.044.464621
GTATCAA137400.037.5878371
ATCAACG176000.029.0113893
TATCAAC178950.028.588932
TCAACGC178950.028.4231934
CAACGCA182450.027.851915
AACGCAG186250.027.257646
GTACATG126100.024.2270411
ACGCAGA212550.023.7284987
CGCAGAG213700.023.5785838
TACATGG129000.023.0882872
ACATGGG128700.022.1434573
GCAGAGT226450.021.7267279
GAGTACT126600.021.70207212-13
AGTACTT132850.020.69896312-13
AGAGTAC196900.020.6459510-11
GTACTTT137350.020.03961214-15
CATGGGA76550.019.3563964
TGGTATC45450.018.812762
CAGAGTA221300.018.73439610-11
Produced by FastQC (version 0.11.5)