##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936954_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2637544 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.12194033540293 31.0 31.0 34.0 31.0 34.0 2 32.18496601383711 33.0 31.0 34.0 30.0 34.0 3 32.3251104057411 34.0 31.0 34.0 30.0 34.0 4 36.02804275492655 37.0 35.0 37.0 35.0 37.0 5 35.93484582626868 37.0 35.0 37.0 35.0 37.0 6 35.96896279265862 37.0 35.0 37.0 35.0 37.0 7 35.91788497177677 37.0 35.0 37.0 35.0 37.0 8 35.89616173227821 37.0 35.0 37.0 35.0 37.0 9 37.57514301183222 39.0 37.0 39.0 35.0 39.0 10-11 37.47313637232213 39.0 37.0 39.0 34.5 39.0 12-13 37.39180825040265 39.0 37.0 39.0 34.0 39.0 14-15 38.59021688358564 40.0 38.0 41.0 34.0 41.0 16-17 38.508961177519694 40.0 38.0 41.0 34.0 41.0 18-19 38.54819066525525 40.0 38.0 41.0 34.0 41.0 20-21 38.64322585708523 40.0 38.0 41.0 34.0 41.0 22-23 38.57885934793884 40.0 38.0 41.0 34.0 41.0 24-25 38.49503249993175 40.0 38.0 41.0 34.0 41.0 26-27 38.31999712611429 40.0 38.0 41.0 34.0 41.0 28-29 38.15726998298417 40.0 38.0 41.0 33.5 41.0 30-31 38.098615226892896 40.0 37.5 41.0 34.0 41.0 32-33 38.01124246647639 40.0 37.0 41.0 33.0 41.0 34-35 37.88763997870747 40.0 37.0 41.0 33.0 41.0 36-37 37.704166262250034 40.0 37.0 41.0 33.0 41.0 38-39 37.56216237530066 40.0 37.0 41.0 33.0 41.0 40-41 37.329993167886485 40.0 36.5 41.0 32.0 41.0 42-43 37.08651514439191 40.0 36.0 41.0 31.5 41.0 44-45 36.823142666056 39.0 36.0 41.0 31.0 41.0 46-47 36.442037744204455 39.0 36.0 41.0 30.0 41.0 48-49 36.058779872487435 39.0 35.0 41.0 29.5 41.0 50-51 35.803850096908334 39.0 35.0 40.0 28.5 41.0 52-53 35.57120279320459 39.0 35.0 40.0 28.0 41.0 54-55 35.29124992796329 38.0 35.0 40.0 27.0 41.0 56-57 35.041282913195005 38.0 34.0 40.0 27.0 41.0 58-59 34.69850114348803 38.0 34.0 40.0 26.0 41.0 60-61 34.36300645600605 37.5 34.0 40.0 26.0 41.0 62-63 34.02440262607941 37.0 33.5 40.0 25.5 41.0 64-65 33.61482841613258 36.5 33.0 39.0 24.5 40.5 66-67 33.186758590567585 36.0 32.5 39.0 23.0 40.0 68-69 32.752100818033746 35.5 32.0 38.5 22.0 40.0 70-71 32.356138892848804 35.0 32.0 38.0 22.0 40.0 72-73 31.96433367557091 35.0 31.0 37.5 20.5 39.5 74-75 31.485653699047297 35.0 31.0 37.0 19.0 39.0 76-77 30.03291546984619 33.5 29.5 35.5 18.0 37.5 78-79 30.745641968437305 34.0 30.5 36.0 18.0 38.0 80-81 30.47288917265456 34.0 30.0 35.5 13.0 37.0 82-83 30.271953188269087 34.0 30.0 35.0 14.0 37.0 84-85 29.93861941260506 34.0 30.0 35.0 9.5 36.5 86-87 29.56972566144868 34.0 30.0 35.0 7.0 36.0 88-89 29.197963901265723 34.0 29.0 35.0 2.0 36.0 90-91 28.74803301859609 34.0 29.0 35.0 2.0 35.0 92-93 28.30562352703879 33.5 29.0 35.0 2.0 35.0 94-95 27.807405866973212 33.0 27.5 35.0 2.0 35.0 96-97 27.171242451310764 33.0 26.5 35.0 2.0 35.0 98-99 26.381201602703122 33.0 25.0 34.0 2.0 35.0 100-101 23.17237229028217 29.0 11.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2732090263076259 1101 2 0.15958644147195145 1101 3 0.18209628130297517 1101 4 0.14005963665061216 1101 5 0.20973303153306233 1101 6 0.19269989772649865 1101 7 0.15056908778129952 1101 8 0.18246610029711974 1101 9 0.19132097641851686 1101 10-11 0.22621580787372864 1101 12-13 0.21076115420196118 1101 14-15 0.28503563736128257 1101 16-17 0.29895517168251473 1101 18-19 0.22965595139002914 1101 20-21 0.2614072701666217 1101 22-23 0.20867865392045104 1101 24-25 0.33734518151167947 1101 26-27 0.25028064502665615 1101 28-29 0.15875852882336972 1101 30-31 0.22512809811370005 1101 32-33 0.33682604830844554 1101 34-35 0.2539724300261028 1101 36-37 0.3150366868886678 1101 38-39 0.32949076873553196 1101 40-41 0.4015243103176047 1101 42-43 0.41141629202298446 1101 44-45 0.3267632305998873 1101 46-47 0.29590008684727565 1101 48-49 0.41147984026195417 1101 50-51 0.23502369921458666 1101 52-53 0.1796846005551913 1101 54-55 0.12416806549569515 1101 56-57 0.1915529358734389 1101 58-59 0.2873040940302971 1101 60-61 0.09609884989422568 1101 62-63 0.038798907177458375 1101 64-65 0.09353507040953701 1101 66-67 0.05453709170807031 1101 68-69 -0.019943443669617977 1101 70-71 0.13239439619796656 1101 72-73 0.0899718031436123 1101 74-75 0.007996905530763598 1101 76-77 -0.06766496115454501 1101 78-79 0.2173769443869844 1101 80-81 0.5167519995357885 1101 82-83 0.42802319016374923 1101 84-85 0.3536471885017498 1101 86-87 0.28830931538164606 1101 88-89 0.20640332062969335 1101 90-91 0.19433170485743645 1101 92-93 0.38928640274503223 1101 94-95 0.4324435284968793 1101 96-97 0.35790157081803997 1101 98-99 0.3523347506831307 1101 100-101 0.610864920202868 1102 1 0.31049673755828877 1102 2 0.26755100973775825 1102 3 0.17600478799680275 1102 4 0.15880748414711832 1102 5 0.2763906607382296 1102 6 0.31295663490978853 1102 7 0.3152113630721445 1102 8 0.2445248828777764 1102 9 0.27261172132517686 1102 10-11 0.36203193949825163 1102 12-13 0.2842412730079431 1102 14-15 0.31250359711771836 1102 16-17 0.38552019133288695 1102 18-19 0.4470804824801178 1102 20-21 0.31242715990958914 1102 22-23 0.4161056153549154 1102 24-25 0.24607927741135427 1102 26-27 0.43056328552864187 1102 28-29 0.46088062248566786 1102 30-31 0.47040187213320195 1102 32-33 0.45598790924389476 1102 34-35 0.4704677513078579 1102 36-37 0.47782823661791696 1102 38-39 0.46116017106621854 1102 40-41 0.6193406886403992 1102 42-43 0.5860273758588761 1102 44-45 0.48776841614873234 1102 46-47 0.48005265385273077 1102 48-49 0.565473360157803 1102 50-51 0.5186303546943449 1102 52-53 0.42903384226331553 1102 54-55 0.5432279496696282 1102 56-57 0.5248482263789711 1102 58-59 0.4747585098072591 1102 60-61 0.5453481910144333 1102 62-63 0.5314764261256002 1102 64-65 0.47192216442601165 1102 66-67 0.5251579531002761 1102 68-69 0.4308005592419093 1102 70-71 0.4236777645819956 1102 72-73 0.45979773430747173 1102 74-75 0.4934581335996526 1102 76-77 0.4885649030148542 1102 78-79 0.6395801553726272 1102 80-81 0.6626672501299424 1102 82-83 0.6252799542151664 1102 84-85 0.5375679568325289 1102 86-87 0.5082041266574642 1102 88-89 0.5294294291231978 1102 90-91 0.523323881346851 1102 92-93 0.6991858426666759 1102 94-95 0.880560561180733 1102 96-97 0.999961244932063 1102 98-99 1.1761792990176758 1102 100-101 1.3538498960337648 1103 1 0.20723790162264777 1103 2 0.13468314340219223 1103 3 0.2114732598363318 1103 4 0.1293387741880494 1103 5 0.18140506130064438 1103 6 0.20398151273178655 1103 7 0.16817963647873313 1103 8 0.11858516113746731 1103 9 0.15691822652411958 1103 10-11 0.09732026256976667 1103 12-13 0.22180647240470108 1103 14-15 0.29799885740504806 1103 16-17 0.33118995922303895 1103 18-19 0.30551416618934013 1103 20-21 0.24019685863168405 1103 22-23 0.23989219788601446 1103 24-25 0.2632543576349633 1103 26-27 0.3278267534562147 1103 28-29 0.3166347527257116 1103 30-31 0.34597661898945375 1103 32-33 0.28646522552333664 1103 34-35 0.38223312419170696 1103 36-37 0.4329738864007737 1103 38-39 0.3876365121894665 1103 40-41 0.5023332842567285 1103 42-43 0.31576028511265264 1103 44-45 0.3631922122277018 1103 46-47 0.42758414799612154 1103 48-49 0.5137411822711755 1103 50-51 0.460001214532646 1103 52-53 0.45987173955442273 1103 54-55 0.4390988435250165 1103 56-57 0.38008005275581525 1103 58-59 0.5066877883050651 1103 60-61 0.4873816751568043 1103 62-63 0.4516588993938413 1103 64-65 0.48274384691061556 1103 66-67 0.37513697590306094 1103 68-69 0.3915944611139821 1103 70-71 0.4828313346741808 1103 72-73 0.43227154115851363 1103 74-75 0.3829153911429408 1103 76-77 0.29064292117997326 1103 78-79 0.46180691320506284 1103 80-81 0.6407758439773872 1103 82-83 0.5178200093228327 1103 84-85 0.5278582075383831 1103 86-87 0.5280126955590561 1103 88-89 0.2779603166287252 1103 90-91 0.3319778256268755 1103 92-93 0.45975452314427656 1103 94-95 0.6567338668468459 1103 96-97 0.8106307009990097 1103 98-99 1.0509291317670488 1103 100-101 1.2798103114956625 1104 1 0.15220054908343172 1104 2 0.09767483072710803 1104 3 0.10066724802922522 1104 4 0.15818684498226787 1104 5 0.07065273299702568 1104 6 0.10134948391923615 1104 7 0.0964790874863013 1104 8 0.09473221807039067 1104 9 0.17861928789705672 1104 10-11 0.08190546958546463 1104 12-13 0.1008506102592861 1104 14-15 0.19510301085462345 1104 16-17 0.24189082822037733 1104 18-19 0.24091998552769667 1104 20-21 0.2433612158596432 1104 22-23 0.08803524940075391 1104 24-25 0.1049091580348005 1104 26-27 0.191001302398341 1104 28-29 0.17674654716611116 1104 30-31 0.28448076833998215 1104 32-33 0.17573013183689312 1104 34-35 0.21044870782571934 1104 36-37 0.2256682253414155 1104 38-39 0.15993484620752696 1104 40-41 0.16462445426365235 1104 42-43 0.25523964595988957 1104 44-45 0.16460587005627048 1104 46-47 0.2168900214604932 1104 48-49 0.3314572158684257 1104 50-51 0.21835738373373914 1104 52-53 0.20051427403064537 1104 54-55 0.20810233796009925 1104 56-57 0.10687166108097301 1104 58-59 0.19434167713340145 1104 60-61 0.2595344465298197 1104 62-63 0.29246664164967484 1104 64-65 0.2910273628843143 1104 66-67 0.22814933079456523 1104 68-69 0.27552222570960794 1104 70-71 0.3430043818756019 1104 72-73 0.303650350125352 1104 74-75 0.3226266041711945 1104 76-77 0.37413189009338055 1104 78-79 0.5976280402372289 1104 80-81 0.5456328385768963 1104 82-83 0.5732898575929894 1104 84-85 0.39675418358133285 1104 86-87 0.43869271998563164 1104 88-89 0.21584879421580538 1104 90-91 0.10112432080444478 1104 92-93 0.29039558802003285 1104 94-95 0.33064489523179574 1104 96-97 0.3110460785628888 1104 98-99 0.652344082191906 1104 100-101 0.7511343539729367 1105 1 0.22330857367286683 1105 2 0.20545558278202947 1105 3 0.20191995799349627 1105 4 0.09462729937035874 1105 5 0.11110682863026256 1105 6 0.1606197582174005 1105 7 0.1651411831058951 1105 8 0.18269853268592584 1105 9 0.0522861792288154 1105 10-11 0.13085402599141105 1105 12-13 0.14688055144220158 1105 14-15 0.3108596579822134 1105 16-17 0.34761145297537155 1105 18-19 0.30532467563791954 1105 20-21 0.2857016696262633 1105 22-23 0.02591155378144805 1105 24-25 0.18655361389055258 1105 26-27 0.23857664370059695 1105 28-29 0.17736814360269904 1105 30-31 0.18688098846384804 1105 32-33 0.23175661347797671 1105 34-35 0.20298418884521396 1105 36-37 0.09589373165736248 1105 38-39 0.31858774034761694 1105 40-41 0.3851577550040943 1105 42-43 0.24702625011190094 1105 44-45 0.2523368773361767 1105 46-47 0.35969129253442844 1105 48-49 0.3805656496562193 1105 50-51 0.2636673283384212 1105 52-53 0.16461784142877178 1105 54-55 0.1959274681742471 1105 56-57 0.06369891880977008 1105 58-59 0.2530539647459875 1105 60-61 0.2834518956959542 1105 62-63 0.35138663581512475 1105 64-65 0.26492049533431583 1105 66-67 0.34174617097409055 1105 68-69 0.23111457689295634 1105 70-71 0.3121008882257996 1105 72-73 0.2996092421409742 1105 74-75 0.3217891976406655 1105 76-77 0.27937273647351546 1105 78-79 0.33673234182836254 1105 80-81 0.4301627399732517 1105 82-83 0.3831563514491023 1105 84-85 0.09334718682555021 1105 86-87 0.06493291211145191 1105 88-89 0.09358402874142158 1105 90-91 0.26850929835032744 1105 92-93 0.2890651449903032 1105 94-95 0.2562024767403166 1105 96-97 0.4581109089630857 1105 98-99 0.5406005157637672 1105 100-101 0.5419736115228453 1106 1 0.05498278930492262 1106 2 0.017921844729961833 1106 3 0.04164408398622754 1106 4 0.05676125622256478 1106 5 0.050965302235191245 1106 6 0.08473849579533521 1106 7 -0.035305327006454945 1106 8 0.06306487972280195 1106 9 0.11810098063173058 1106 10-11 0.052373891427414776 1106 12-13 0.06920974827767878 1106 14-15 0.10026923491133033 1106 16-17 0.10868329362675411 1106 18-19 0.14453928624304524 1106 20-21 -0.039359283204426276 1106 22-23 -0.0023153186685007654 1106 24-25 0.013606551686521584 1106 26-27 -0.06314410037261098 1106 28-29 -0.016604860864312343 1106 30-31 -0.15697832012008206 1106 32-33 -0.041503884060041685 1106 34-35 -0.03630793734098603 1106 36-37 0.009511717308704704 1106 38-39 0.017725840910394197 1106 40-41 0.12346006195179626 1106 42-43 0.10889119353125665 1106 44-45 0.07446655746667119 1106 46-47 0.1175692112219835 1106 48-49 0.1062667742685619 1106 50-51 -0.029024696148361784 1106 52-53 0.02490338897325728 1106 54-55 0.08311124056100994 1106 56-57 0.07756695266969871 1106 58-59 0.25809960538553156 1106 60-61 0.15710429877442067 1106 62-63 0.12199352935594021 1106 64-65 0.13152725395474363 1106 66-67 0.13796910018480446 1106 68-69 0.16583570217989774 1106 70-71 0.2677058438770814 1106 72-73 0.12581619092277663 1106 74-75 0.2395479027104237 1106 76-77 0.12744047923634483 1106 78-79 0.15391839895187331 1106 80-81 0.4215594650990546 1106 82-83 0.3920124747663394 1106 84-85 0.3035316395420047 1106 86-87 0.37551875298049353 1106 88-89 0.0962604801061211 1106 90-91 0.1806327861441659 1106 92-93 0.3939152783514679 1106 94-95 0.4281630290738363 1106 96-97 0.5284194502509543 1106 98-99 0.4837936231139359 1106 100-101 0.5073802151121107 1107 1 0.0490527606934279 1107 2 0.06790089056525517 1107 3 0.028753392291832824 1107 4 0.13125512260225491 1107 5 0.11574743066093163 1107 6 0.012867004324455422 1107 7 0.0975783540970312 1107 8 0.10219613557573837 1107 9 0.1480598742518353 1107 10-11 0.06611736588661188 1107 12-13 0.14579392193337526 1107 14-15 0.18838382573599688 1107 16-17 0.2655171969478616 1107 18-19 0.19879220559797517 1107 20-21 0.2093351559224388 1107 22-23 0.29706976714009414 1107 24-25 0.24290377228472693 1107 26-27 0.24047133379250596 1107 28-29 0.3300091660087574 1107 30-31 0.36728977160224474 1107 32-33 0.35476161814144547 1107 34-35 0.32139711597572074 1107 36-37 0.417051012834861 1107 38-39 0.30107135439090627 1107 40-41 0.4319750842770347 1107 42-43 0.4394316298952674 1107 44-45 0.4525040059220089 1107 46-47 0.6177019209317791 1107 48-49 0.5042497418956771 1107 50-51 0.3383038160024725 1107 52-53 0.3902100829234527 1107 54-55 0.39711929368628773 1107 56-57 0.409800537280546 1107 58-59 0.513434057136628 1107 60-61 0.5028872084542115 1107 62-63 0.5271071862974637 1107 64-65 0.4292305740257305 1107 66-67 0.4726813555255234 1107 68-69 0.5914794606844396 1107 70-71 0.46725209566853465 1107 72-73 0.44013803913184546 1107 74-75 0.49670724231793884 1107 76-77 0.6259004128038796 1107 78-79 0.6949200080247699 1107 80-81 0.7067927289301004 1107 82-83 0.719190349755344 1107 84-85 0.560369746630716 1107 86-87 0.5742906061047286 1107 88-89 0.4582967075286568 1107 90-91 0.4303266225154232 1107 92-93 0.6126006373402859 1107 94-95 0.7577380217010763 1107 96-97 0.7597181169452121 1107 98-99 0.9605810651019446 1107 100-101 0.9148830995434274 1108 1 0.12036346621162863 1108 2 0.06845911449523356 1108 3 0.05511372957436578 1108 4 0.059542026220569255 1108 5 0.005944356693596831 1108 6 0.04384149466494591 1108 7 0.04116518300086369 1108 8 0.07361677812030365 1108 9 0.07078001514200594 1108 10-11 0.08753480638186772 1108 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-1.030511080997492 2316 52-53 -1.1281131773453623 2316 54-55 -0.8362253954107004 2316 56-57 -1.0087807516629397 2316 58-59 -1.0185084550366739 2316 60-61 -0.8739665384720681 2316 62-63 -0.9544255140731863 2316 64-65 -0.8497216017350624 2316 66-67 -0.7276047145732747 2316 68-69 -1.0062056550016294 2316 70-71 -0.697934357337104 2316 72-73 -0.8279055997728442 2316 74-75 -0.8759006471274908 2316 76-77 -0.8180734296482477 2316 78-79 -0.7350150183304685 2316 80-81 -0.8058129616025234 2316 82-83 -0.8951603842375206 2316 84-85 -1.0981569342161173 2316 86-87 -1.0328268412112216 2316 88-89 -0.9749166018139732 2316 90-91 -1.058755193955701 2316 92-93 -0.9808844755971435 2316 94-95 -0.8673407374355158 2316 96-97 -0.9499770270518617 2316 98-99 -1.131917047850287 2316 100-101 -1.419050353743419 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 3.0 4 16.0 5 44.0 6 120.0 7 319.0 8 720.0 9 1447.0 10 2472.0 11 4233.0 12 6352.0 13 8845.0 14 11213.0 15 15718.0 16 20681.0 17 28240.0 18 25296.0 19 15561.0 20 13610.0 21 13673.0 22 14517.0 23 15604.0 24 18349.0 25 21753.0 26 26869.0 27 33359.0 28 41544.0 29 52356.0 30 65722.0 31 82214.0 32 104739.0 33 136420.0 34 185313.0 35 261022.0 36 392755.0 37 575983.0 38 409856.0 39 30601.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.121622590232704 19.616917492692906 14.955888169883355 22.30557174719103 2 15.657862108346801 21.121460741214843 39.682241162138 23.53843598830036 3 19.22791514946067 25.40192450542213 30.1734496818465 25.196710663270704 4 13.03596302921432 17.389182807325913 37.115844979909326 32.45900918355044 5 13.573138849972416 37.88611196948527 33.85568824018973 14.685060940352578 6 31.28228382161587 36.80609688407094 17.365511248343154 14.546108045970039 7 27.85640732438966 33.529260554515865 20.808107845783805 17.80622427531067 8 26.478592568383746 34.83224153397368 19.71350598398054 18.97565991366204 9 26.74600063240699 16.499187501090027 20.492109699106216 36.26270216739677 10-11 24.817462760810816 27.155888205087763 27.10735820900049 20.919290825100926 12-13 26.003947620849445 24.48324915129185 27.953379249891373 21.55942397796733 14-15 22.850670927195907 25.436713093696255 26.659403596679336 25.0532123824285 16-17 21.858961161960202 28.87414536938355 27.140239988504455 22.12665348015179 18-19 21.803957789593678 26.91198721167935 30.141277046219457 21.14277795250751 20-21 22.60599596740254 26.042898101464036 30.3401774251888 21.01092850594463 22-23 22.275419860294274 26.10178256741878 30.21178035323771 21.411017219049235 24-25 22.13940501664233 26.285739960335956 30.21775410380255 21.357100919219164 26-27 21.95981640039441 26.53130325500466 30.057392413160155 21.45148793144078 28-29 21.684268639998727 26.493374471381514 30.303276192801803 21.519080695817955 30-31 21.867286689756344 26.315880297768857 30.23653645726665 21.580296555208147 32-33 21.74322106080112 26.485942979508636 30.126779816915516 21.644056142774733 34-35 22.12954667044817 26.46702411506497 29.919310592982402 21.48411862150446 36-37 22.207169995842047 26.289359070302027 29.77515765811458 21.728313275741346 38-39 21.97576903133949 26.551006653515728 29.932384203327047 21.540840111817737 40-41 22.149746167390173 26.392714131035905 29.89702038415111 21.56051931742281 42-43 22.044703661314 26.502708879060403 29.57718281506917 21.87540464455643 44-45 22.174194274890056 26.73620050131395 29.285348946281452 21.80425627751454 46-47 22.312491315114837 27.00836725296395 28.732718618820318 21.94642281310089 48-49 22.505622282342365 27.134424633359703 28.364947593295387 21.995005491002548 50-51 22.462216331007728 27.29468892099904 28.19394801567727 22.04914673231596 52-53 22.718496186379706 27.308850256942822 28.038436302882193 21.93421725379528 54-55 22.401399208300766 27.264125780120995 28.161114452880703 22.173360558697535 56-57 22.391880853157996 27.660678019888767 27.983064384192947 21.96437674276029 58-59 22.596078199424987 27.250292226047794 28.02498035080775 22.128649223719464 60-61 22.6278120857889 27.208020036822134 28.353612299927512 21.810555577461457 62-63 22.458936680641166 27.250499281054243 28.387182386634528 21.903381651670067 64-65 22.35814074382608 27.27215679077671 28.232657085867547 22.137045379529663 66-67 22.32002032212078 27.5862363129 27.913494494828477 22.18024887015075 68-69 22.365448504983387 27.404116568167925 28.152226766697787 22.0782081601509 70-71 22.417637291087228 27.375921563170575 28.065341527912825 22.141099617829372 72-73 22.496591112472814 27.37791708250797 27.912721862079216 22.21276994294 74-75 22.414998905168645 27.63295565601252 27.6855644353277 22.266481003491137 76-77 22.481078669850824 27.519840784611247 27.799260151962123 22.199820393575802 78-79 22.44160486931647 27.545351235321352 27.685819579417796 22.32722431594438 80-81 22.451291195401467 27.825832931601134 27.477818790375036 22.245057082622363 82-83 22.521916890703046 27.716053630716388 27.55223739685179 22.209792081728775 84-85 22.437801419515303 27.709173769298435 27.41764991355516 22.435374897631107 86-87 22.39822349883073 27.53131322169412 27.610894832465355 22.459568447009794 88-89 22.535559174295404 27.595189393005892 27.473502419900626 22.39574901279808 90-91 22.386182939895857 27.711655106573517 27.57139087370646 22.330771079824167 92-93 22.362504694249612 27.49060628757414 27.580197498763525 22.566691519412725 94-95 22.336255961321292 27.445044565479698 27.542806727598823 22.675892745600184 96-97 22.380404700533322 27.398640508612125 27.597026840068768 22.623927950785784 98-99 22.113048336221446 27.41847455702492 27.42203849009805 23.04643861665559 100-101 21.95108542448929 27.687580343099278 27.45321214104253 22.908122091368906 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 743.0 1 549.0 2 464.5 3 570.0 4 499.5 5 508.5 6 896.0 7 1829.0 8 3078.0 9 3770.0 10 3493.5 11 3143.0 12 3349.0 13 3685.5 14 3992.5 15 4333.5 16 4641.0 17 4919.5 18 5159.5 19 5426.5 20 5971.0 21 6763.5 22 7900.0 23 9408.5 24 11136.0 25 13558.5 26 16910.5 27 20946.5 28 24351.0 29 28015.0 30 32807.0 31 37352.5 32 42028.0 33 47497.5 34 54136.5 35 58416.5 36 63502.5 37 70458.0 38 74000.5 39 74864.5 40 75574.0 41 79144.0 42 82791.5 43 85908.0 44 89758.0 45 92110.5 46 92375.0 47 94994.0 48 101568.0 49 101999.0 50 97572.5 51 93580.5 52 89411.5 53 88101.5 54 86051.0 55 79502.0 56 71881.5 57 64858.0 58 59003.0 59 53574.5 60 46568.0 61 37996.5 62 30349.5 63 23902.5 64 17848.0 65 13300.0 66 9571.5 67 7543.0 68 5513.0 69 3264.5 70 2145.0 71 1464.5 72 1039.0 73 821.0 74 629.0 75 460.5 76 298.0 77 144.0 78 86.0 79 50.5 80 28.0 81 21.5 82 11.5 83 6.5 84 5.0 85 4.5 86 3.0 87 2.5 88 2.0 89 1.5 90 0.5 91 1.0 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.027411864977418383 2 0.0075069837697494335 3 0.011184647535737793 4 7.582811888635792E-5 5 0.0048909136681700854 6 0.0 7 0.0 8 7.582811888635792E-5 9 7.582811888635792E-5 10-11 0.0 12-13 2.2748435665907374E-4 14-15 0.0 16-17 3.791405944317896E-5 18-19 0.005213183173437106 20-21 0.0014407342588408003 22-23 0.0 24-25 0.0054027534706530015 26-27 0.006616003372834728 28-29 0.009288944563578844 30-31 0.032909403596679335 32-33 7.014100996988107E-4 34-35 0.005573366738147307 36-37 0.016284088530845363 38-39 0.008208393869448246 40-41 0.010407409317152623 42-43 0.007525940799471023 44-45 0.002843554458238422 46-47 0.002255886536869148 48-49 0.0018009178235510006 50-51 0.003601835647102001 52-53 0.0033933083201645167 54-55 0.001990488120766895 56-57 9.668085158010634E-4 58-59 0.0011753358427385476 60-61 0.0 62-63 5.6871089164768435E-5 64-65 0.00836005010722096 66-67 9.09937426636295E-4 68-69 0.0011942928724601371 70-71 0.0041515895090280955 72-73 0.012132499021817266 74-75 0.005743980005641612 76-77 0.004189503568471275 78-79 0.011184647535737793 80-81 2.8435544582384217E-4 82-83 0.005213183173437106 84-85 9.09937426636295E-4 86-87 0.0 88-89 0.0014786483182839794 90-91 8.341093077499371E-4 92-93 3.222695052670211E-4 94-95 7.772382185851686E-4 96-97 5.118398024829159E-4 98-99 3.222695052670211E-4 100-101 0.0010426366346874213 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2637544.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.03185981606049 #Duplication Level Percentage of deduplicated Percentage of total 1 71.95649258520632 35.281606572932326 2 14.873900911180455 14.585900487899488 3 5.402507240227266 7.946849329742253 4 2.5364850453679537 4.9747431668006135 5 1.4073807287238467 3.450324729930635 6 0.8423047056262933 2.477985974920591 7 0.571730646332215 1.9623111832454778 8 0.39088159238707926 1.5332521154081413 9 0.3030640772418576 1.3373815815545842 >10 1.524394948046748 13.582114669140891 >50 0.11936962186886793 4.056820767877537 >100 0.06731358629010441 6.080127425042827 >500 0.0033239887878624293 1.0718866610181048 >1k 6.184165186687864E-4 0.45706793653334504 >5k 7.73020648335983E-5 0.2724441767809673 >10k+ 1.546041296671966E-4 0.9291832211721498 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13613 0.5161240911999951 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10902 0.41333907604953696 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7188 0.27252625927757035 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2772 0.10509777277649206 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 1.895702972158948E-5 14-15 0.0 0.0 0.0 0.0 3.791405944317896E-5 16-17 0.0 0.0 0.0 3.791405944317896E-5 3.791405944317896E-5 18-19 0.0 0.0 0.0 5.687108916476844E-5 3.791405944317896E-5 20-21 0.0 0.0 0.0 1.1374217832953687E-4 3.791405944317896E-5 22-23 0.0 0.0 0.0 2.653984161022527E-4 3.791405944317896E-5 24-25 0.0 0.0 0.0 4.1705465387496853E-4 3.791405944317896E-5 26-27 0.0 0.0 0.0 6.066249510908633E-4 5.687108916476844E-5 28-29 0.0 0.0 0.0 0.0013649061399544425 7.582811888635792E-5 30-31 0.0 0.0 0.0 0.0027487693096304743 7.582811888635792E-5 32-33 0.0 0.0 0.0 0.0048909136681700854 1.1374217832953687E-4 34-35 0.0 0.0 0.0 0.007829253275016455 1.1374217832953687E-4 36-37 0.0 0.0 0.0 0.010350538227987855 1.1374217832953687E-4 38-39 0.0 0.0 0.0 0.01273912397290813 1.1374217832953687E-4 40-41 0.0 0.0 0.0 0.01677697130360669 1.1374217832953687E-4 42-43 0.0 0.0 0.0 0.021990154477043795 1.1374217832953687E-4 44-45 0.0 0.0 0.0 0.027146466561316134 1.5165623777271584E-4 46-47 0.0 0.0 0.0 0.0333264582505543 2.0852732693748426E-4 48-49 0.0 0.0 0.0 0.03933583667229817 2.2748435665907374E-4 50-51 0.0 0.0 0.0 0.04769588677951912 2.653984161022527E-4 52-53 0.0 0.0 0.0 0.07161965828816505 2.653984161022527E-4 54-55 0.0 0.0 0.0 0.1065005929758897 2.653984161022527E-4 56-57 0.0 0.0 0.0 0.13086037616813218 2.653984161022527E-4 58-59 0.0 0.0 0.0 0.1709734510590155 2.653984161022527E-4 60-61 0.0 0.0 0.0 0.21512437328059741 2.653984161022527E-4 62-63 0.0 0.0 0.0 0.24204335548525446 2.653984161022527E-4 64-65 0.0 0.0 0.0 0.262289463227912 2.653984161022527E-4 66-67 0.0 0.0 0.0 0.3292077781451229 2.653984161022527E-4 68-69 0.0 0.0 0.0 0.4055098227745205 2.653984161022527E-4 70-71 0.0 0.0 0.0 0.432732117454723 2.653984161022527E-4 72-73 0.0 0.0 0.0 0.4932808703854798 3.0331247554543167E-4 74-75 0.0 0.0 0.0 0.5848054098813138 3.412265349886106E-4 76-77 0.0 0.0 0.0 0.6703963990742903 3.412265349886106E-4 78-79 0.0 0.0 0.0 0.754546654008426 3.412265349886106E-4 80-81 0.0 0.0 0.0 0.8771796792773884 3.412265349886106E-4 82-83 0.0 0.0 0.0 1.0171773437713267 3.791405944317896E-4 84-85 0.0 0.0 0.0 1.1451752084514988 3.791405944317896E-4 86-87 0.0 0.0 0.0 1.3727543502591806 3.791405944317896E-4 88-89 0.0 0.0 0.0 1.6497544685510461 3.791405944317896E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8185 0.0 44.46462 1 GTATCAA 13740 0.0 37.587837 1 ATCAACG 17600 0.0 29.011389 3 TATCAAC 17895 0.0 28.58893 2 TCAACGC 17895 0.0 28.423193 4 CAACGCA 18245 0.0 27.85191 5 AACGCAG 18625 0.0 27.25764 6 GTACATG 12610 0.0 24.227041 1 ACGCAGA 21255 0.0 23.728498 7 CGCAGAG 21370 0.0 23.578583 8 TACATGG 12900 0.0 23.088287 2 ACATGGG 12870 0.0 22.143457 3 GCAGAGT 22645 0.0 21.726727 9 GAGTACT 12660 0.0 21.702072 12-13 AGTACTT 13285 0.0 20.698963 12-13 AGAGTAC 19690 0.0 20.64595 10-11 GTACTTT 13735 0.0 20.039612 14-15 CATGGGA 7655 0.0 19.356396 4 TGGTATC 4545 0.0 18.81276 2 CAGAGTA 22130 0.0 18.734396 10-11 >>END_MODULE